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The map label for this gene is sseA [H]

Identifier: 83592950

GI number: 83592950

Start: 1908496

End: 1909353

Strand: Reverse

Name: sseA [H]

Synonym: Rru_A1615

Alternate gene names: 83592950

Gene position: 1909353-1908496 (Counterclockwise)

Preceding gene: 83592951

Following gene: 83592948

Centisome position: 43.86

GC content: 67.72

Gene sequence:

>858_bases
ATGACCCGTTCGCGCACCGAAGCCCTGGTCACCCCCCAGTGGCTCCACGATCATCTCGACGCCCCCGATCTGCGCATCAT
CGATGCCAGCTATTTCATGCCGGCCAGCGGCCGCGACGCCCACCAGGAGTACGAGGCCGCCCATATTCCCGGGGCGATCT
TCTTTGATATCAACGCCGTAGCCGACACCAGCCAACCCAAGCCGCATAGCCTGCCGGCCCCCGAGGTTTTCTCGTCGGCC
TGCCGGGGATTGGGGATCGGCGATGGTCAGCGACTCGTCGTCTATGACCGCAACGGCGGGGCCATGGCCGCGGCCCGGGT
GTGGTGGATGTTCCGGCTGTTTGGTCAGACCGAGGTCTCGGTTCTTGATGGCGGCCTGGGAGCCTGGCAGGCCGAGGGCC
GCCCGACCGACGATCTGCCCCGCCTGCATGGGCGACGCCATTTCACCGCCCGCCTGGACAATACCCTGATGCGCACGGCG
TCCCAGGTGCGCGAGGCGGTGGAAAGCGGCCGGGCCCAGGTGGTCGACGCCCGCTCAAGCGGCCGTTTCACCGCCACCGC
CCCCGAACCCTGGCCGGTCGCCCGGGTCGGGCATATTCCGGGATCGATCAACCTGCCTTACACCGATCTGGTGGTGGCCA
GCGAGCATGGCCGGCTGGCCGACCGCGAGATCCTGACCCAGGCCTTCGCCAAAGCCGGAGTCGATCTTGGCAAACCGCTG
ATCGCCACCTGCGGCAGCGGGGTCAGCGCCTGCGTCATCGCCCTGGCCGCCGCCCAGCTTGGGCACGAGACCGTCGCGGT
CTATGACGGATCGTGGTTCGAATGGAGCGGTCTAGCCGATTATCCGACCGCTCCCTAA

Upstream 100 bases:

>100_bases
CGCTCGAAAGCCCTGCCGGTTCCCGACTGGCTGGCCTGAGCCGGCGATCCCGGTCCACCGGCCAACAGACCATGATAGTT
CACGAAAGGAGCCGACGTTC

Downstream 100 bases:

>100_bases
CGGCCCGCCCAGCGCCGCGACGGCGGTTTATTGGGCGGCTTCGCCACGGGCCAGCCCGTGATCGGCCTCGTCATCGTCCA
TATCCAGGCCGAACTGGCCG

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTRSRTEALVTPQWLHDHLDAPDLRIIDASYFMPASGRDAHQEYEAAHIPGAIFFDINAVADTSQPKPHSLPAPEVFSSA
CRGLGIGDGQRLVVYDRNGGAMAAARVWWMFRLFGQTEVSVLDGGLGAWQAEGRPTDDLPRLHGRRHFTARLDNTLMRTA
SQVREAVESGRAQVVDARSSGRFTATAPEPWPVARVGHIPGSINLPYTDLVVASEHGRLADREILTQAFAKAGVDLGKPL
IATCGSGVSACVIALAAAQLGHETVAVYDGSWFEWSGLADYPTAP

Sequences:

>Translated_285_residues
MTRSRTEALVTPQWLHDHLDAPDLRIIDASYFMPASGRDAHQEYEAAHIPGAIFFDINAVADTSQPKPHSLPAPEVFSSA
CRGLGIGDGQRLVVYDRNGGAMAAARVWWMFRLFGQTEVSVLDGGLGAWQAEGRPTDDLPRLHGRRHFTARLDNTLMRTA
SQVREAVESGRAQVVDARSSGRFTATAPEPWPVARVGHIPGSINLPYTDLVVASEHGRLADREILTQAFAKAGVDLGKPL
IATCGSGVSACVIALAAAQLGHETVAVYDGSWFEWSGLADYPTAP
>Mature_284_residues
TRSRTEALVTPQWLHDHLDAPDLRIIDASYFMPASGRDAHQEYEAAHIPGAIFFDINAVADTSQPKPHSLPAPEVFSSAC
RGLGIGDGQRLVVYDRNGGAMAAARVWWMFRLFGQTEVSVLDGGLGAWQAEGRPTDDLPRLHGRRHFTARLDNTLMRTAS
QVREAVESGRAQVVDARSSGRFTATAPEPWPVARVGHIPGSINLPYTDLVVASEHGRLADREILTQAFAKAGVDLGKPLI
ATCGSGVSACVIALAAAQLGHETVAVYDGSWFEWSGLADYPTAP

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=273, Percent_Identity=44.6886446886447, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI61835204, Length=273, Percent_Identity=44.6886446886447, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI194473681, Length=273, Percent_Identity=44.6886446886447, Blast_Score=233, Evalue=1e-61,
Organism=Homo sapiens, GI17402865, Length=274, Percent_Identity=45.2554744525547, Blast_Score=217, Evalue=8e-57,
Organism=Escherichia coli, GI87082121, Length=286, Percent_Identity=42.3076923076923, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI87081967, Length=298, Percent_Identity=27.5167785234899, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17561888, Length=300, Percent_Identity=30.6666666666667, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71997283, Length=304, Percent_Identity=29.6052631578947, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17543836, Length=280, Percent_Identity=29.2857142857143, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17543838, Length=280, Percent_Identity=29.2857142857143, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI115534702, Length=209, Percent_Identity=29.1866028708134, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17533113, Length=154, Percent_Identity=33.7662337662338, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17559150, Length=248, Percent_Identity=22.5806451612903, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324825, Length=268, Percent_Identity=31.3432835820896, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30722; Mature: 30591

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSRTEALVTPQWLHDHLDAPDLRIIDASYFMPASGRDAHQEYEAAHIPGAIFFDINAV
CCCCCCHHCCCHHHHHHCCCCCCEEEEECCEECCCCCCCHHHHHHHHCCCEEEEEEEEEC
ADTSQPKPHSLPAPEVFSSACRGLGIGDGQRLVVYDRNGGAMAAARVWWMFRLFGQTEVS
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEE
VLDGGLGAWQAEGRPTDDLPRLHGRRHFTARLDNTLMRTASQVREAVESGRAQVVDARSS
EEECCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GRFTATAPEPWPVARVGHIPGSINLPYTDLVVASEHGRLADREILTQAFAKAGVDLGKPL
CCEEEECCCCCCCCEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHH
IATCGSGVSACVIALAAAQLGHETVAVYDGSWFEWSGLADYPTAP
HHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCC
>Mature Secondary Structure 
TRSRTEALVTPQWLHDHLDAPDLRIIDASYFMPASGRDAHQEYEAAHIPGAIFFDINAV
CCCCCHHCCCHHHHHHCCCCCCEEEEECCEECCCCCCCHHHHHHHHCCCEEEEEEEEEC
ADTSQPKPHSLPAPEVFSSACRGLGIGDGQRLVVYDRNGGAMAAARVWWMFRLFGQTEVS
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEE
VLDGGLGAWQAEGRPTDDLPRLHGRRHFTARLDNTLMRTASQVREAVESGRAQVVDARSS
EEECCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GRFTATAPEPWPVARVGHIPGSINLPYTDLVVASEHGRLADREILTQAFAKAGVDLGKPL
CCEEEECCCCCCCCEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHH
IATCGSGVSACVIALAAAQLGHETVAVYDGSWFEWSGLADYPTAP
HHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]