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The map label for this gene is aam1 [H]

Identifier: 83592939

GI number: 83592939

Start: 1891159

End: 1893225

Strand: Direct

Name: aam1 [H]

Synonym: Rru_A1604

Alternate gene names: 83592939

Gene position: 1891159-1893225 (Clockwise)

Preceding gene: 83592937

Following gene: 83592940

Centisome position: 43.45

GC content: 65.07

Gene sequence:

>2067_bases
ATGTCTTCCGCCCTCCCGCCTTTTCGCCCCTCTCTCGTCTCCGACCCGTTGTCGGCAAGGCAAGACCCCTCCCTTCCCGG
CATTCTCCCGGTCAGCGCGCCGATCCTGATCGAGGATATCGGGCCGCGGGTGAGCGACGGCCGCTATCCGATCAAGCGGG
TCTTGGGCGAGGGCGTGGTGGTCAGCGCGGCGGTTTTTCGCGATGGCCATGTGCCGCTGGCCGCCGCCCTGCTGTGGCGC
CGCTCCCCCCGGCCCGGGGAGCCCGCCGGGGGGCGGGGGGCGTGGCACGAGGTGCCCCTGACCCAGATCGAACCGGGGCT
GGCGCGCTGGCGGGCCCGCTTCGATCCTCCCGCCATCGGTCGCTTTGATTACCTCATCGAGGCCTGGAGCGATGACTGGG
CGGGCTGGCGCCACGATACCCTGATCAAACGCGACGCCGGGCTTGATCTTTCGCTGGAGGTCCTGGAGGGCATGGCCTTG
ATCGAGGCGACGATCGCCCGCATCGACGGCACGGACCAAGCCGCCGATCAAGGCTTGCTGAGCTCTTGGCTGGCCGATGC
CCAAGGGCTGGAAACGGCCGGCGGCCGGGCGACCCTGATGCTGTCGGAGACCCTTGACGCGGTGATGGCCCGCCATCCCG
ATCGCTCCGGGGCGGTGCGCTCGGGCCCGCCCTTGTCGGTGGTCGTCGATCCGCCGCGCGCCGGCTTCGCCGCTTGGTAT
GAGATGGCGCCGCGCTCCCAGGCCAGCGAGCCCGGGCGCTGGGGGACCTTCGCCGATTGCGCCGCCCGCCTGCCCGATAT
TCGCGCCATGGGCTTTGACACCTTGTATCTGATGCCCATCCATCCGATCGGTCGCATCCACCGCAAGGGCCCCAACAACA
GCCTGAAGGCGGGGCCCGACGATCCGGGCAGTCCCTATGCCATCGGCGCGGCCGAGGGCGGCCATACCGCCATCCACCCC
GAATTGGGAACGCTCGATGAGTTTCGCGCCCTGGTCGCCCAGGCCGGGGAAATGGGCATGGATGTGGCCCTTGATATCGC
CATCCAATGCGCCCCCGACCACCCCTGGGTGAGCGAGCATCCCGAGTGGTTCGAATTTCGGCCCGACGGCACCATCAAAT
ATGCCGAAAACCCGCCCAAGAAATATCAAGACATCGTCAATCTCGCCTTCTGGGGACCCCACCGCCAAGAGCTGTGGCTG
GCCCTGCTTGAGGTTTTCACCTTCTGGGCCGAGCAGGGCGTGCGCGTCTTCCGCGTCGACAATCCCCACACCAAACCGGT
GCCGTTCTGGGAATGGCTGATCGCCACGGTGAAGGCCCGCTATCCCGACTGCATTTTTCTGGCCGAGGCCTTTACCCGGC
CGCCGATGATGAAAATGCTGGCCAAGATCGGCTTCACCCAATCCTACAGCTATTTCACCTGGAGGACGACCAAGGCCGAG
CTGACCAGCTATATGGAAGAGCTGGTCGAGGATGATCCCGCCGATTACATGCGGGTCAATTTCTTCGCCAATACCCCCGA
TATCTTGCCCTTTCACCTGCAGACCGGCGGTCGGCCGGCCTTCATGCAGCGCCTTGTTCTCGCCGCCACGCTGTCAAGCG
TCTATGGCATCCATAACGGCTTCGAGCTGTGCGAGAACGCGGCGATCCCGGGCAAGGAGGAATACGCCGACAGCGAGAAG
TATGACTTCAAGCCCCGCGACTGGAACGCCGAGGGCAACATCAAGGATCTGATCGCCAAGGTGAACTGGATCCGCAACGA
AAACCCGGCCCTGCAAGCCTTTCGGACCCTGCGGTTTTTCCGCGCCGACAACGACCAGATCCTGTTCTACGGCAAGATGA
CCGGGGATCGGGCCAATATCATCCTCGTGGCCGTCAATCTTGATCCCCATGCCGGCCAGGGCGGGCTGCTGTGGTTGCCG
CTGGCCGAAATGGGCCTCAGCGATGGACAGCCCTTCATTGTCGAAGAGTTGCTGTCGGGAACCCGGCTGGAATGGACGGG
CAGCCCCCATCACTACTGGTTCGATCCGGCGACCAACCCCGCCGCCATCTTTCGCATCACCCCCTGA

Upstream 100 bases:

>100_bases
TGATCTGCAACTCTTTCCCCCCTGCGGACGTTCCATTGAGGATTCCCGGCTTCAAAAGGTCCCGGCTCTCCGGGGCTCCT
CGAGAAGGAACGAATGATCG

Downstream 100 bases:

>100_bases
GTCCCGTTTCGCCGGCAACCGGAGCCTTCCATGAGCGACTTCCTATGGTACAAGGACGCCATCGTCTATCAGCTTCATGT
AAAAGCCTTTTTTGATTCGA

Product: alpha amylase, catalytic region

Products: D-glucose; cholesterol

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSSALPPFRPSLVSDPLSARQDPSLPGILPVSAPILIEDIGPRVSDGRYPIKRVLGEGVVVSAAVFRDGHVPLAAALLWR
RSPRPGEPAGGRGAWHEVPLTQIEPGLARWRARFDPPAIGRFDYLIEAWSDDWAGWRHDTLIKRDAGLDLSLEVLEGMAL
IEATIARIDGTDQAADQGLLSSWLADAQGLETAGGRATLMLSETLDAVMARHPDRSGAVRSGPPLSVVVDPPRAGFAAWY
EMAPRSQASEPGRWGTFADCAARLPDIRAMGFDTLYLMPIHPIGRIHRKGPNNSLKAGPDDPGSPYAIGAAEGGHTAIHP
ELGTLDEFRALVAQAGEMGMDVALDIAIQCAPDHPWVSEHPEWFEFRPDGTIKYAENPPKKYQDIVNLAFWGPHRQELWL
ALLEVFTFWAEQGVRVFRVDNPHTKPVPFWEWLIATVKARYPDCIFLAEAFTRPPMMKMLAKIGFTQSYSYFTWRTTKAE
LTSYMEELVEDDPADYMRVNFFANTPDILPFHLQTGGRPAFMQRLVLAATLSSVYGIHNGFELCENAAIPGKEEYADSEK
YDFKPRDWNAEGNIKDLIAKVNWIRNENPALQAFRTLRFFRADNDQILFYGKMTGDRANIILVAVNLDPHAGQGGLLWLP
LAEMGLSDGQPFIVEELLSGTRLEWTGSPHHYWFDPATNPAAIFRITP

Sequences:

>Translated_688_residues
MSSALPPFRPSLVSDPLSARQDPSLPGILPVSAPILIEDIGPRVSDGRYPIKRVLGEGVVVSAAVFRDGHVPLAAALLWR
RSPRPGEPAGGRGAWHEVPLTQIEPGLARWRARFDPPAIGRFDYLIEAWSDDWAGWRHDTLIKRDAGLDLSLEVLEGMAL
IEATIARIDGTDQAADQGLLSSWLADAQGLETAGGRATLMLSETLDAVMARHPDRSGAVRSGPPLSVVVDPPRAGFAAWY
EMAPRSQASEPGRWGTFADCAARLPDIRAMGFDTLYLMPIHPIGRIHRKGPNNSLKAGPDDPGSPYAIGAAEGGHTAIHP
ELGTLDEFRALVAQAGEMGMDVALDIAIQCAPDHPWVSEHPEWFEFRPDGTIKYAENPPKKYQDIVNLAFWGPHRQELWL
ALLEVFTFWAEQGVRVFRVDNPHTKPVPFWEWLIATVKARYPDCIFLAEAFTRPPMMKMLAKIGFTQSYSYFTWRTTKAE
LTSYMEELVEDDPADYMRVNFFANTPDILPFHLQTGGRPAFMQRLVLAATLSSVYGIHNGFELCENAAIPGKEEYADSEK
YDFKPRDWNAEGNIKDLIAKVNWIRNENPALQAFRTLRFFRADNDQILFYGKMTGDRANIILVAVNLDPHAGQGGLLWLP
LAEMGLSDGQPFIVEELLSGTRLEWTGSPHHYWFDPATNPAAIFRITP
>Mature_687_residues
SSALPPFRPSLVSDPLSARQDPSLPGILPVSAPILIEDIGPRVSDGRYPIKRVLGEGVVVSAAVFRDGHVPLAAALLWRR
SPRPGEPAGGRGAWHEVPLTQIEPGLARWRARFDPPAIGRFDYLIEAWSDDWAGWRHDTLIKRDAGLDLSLEVLEGMALI
EATIARIDGTDQAADQGLLSSWLADAQGLETAGGRATLMLSETLDAVMARHPDRSGAVRSGPPLSVVVDPPRAGFAAWYE
MAPRSQASEPGRWGTFADCAARLPDIRAMGFDTLYLMPIHPIGRIHRKGPNNSLKAGPDDPGSPYAIGAAEGGHTAIHPE
LGTLDEFRALVAQAGEMGMDVALDIAIQCAPDHPWVSEHPEWFEFRPDGTIKYAENPPKKYQDIVNLAFWGPHRQELWLA
LLEVFTFWAEQGVRVFRVDNPHTKPVPFWEWLIATVKARYPDCIFLAEAFTRPPMMKMLAKIGFTQSYSYFTWRTTKAEL
TSYMEELVEDDPADYMRVNFFANTPDILPFHLQTGGRPAFMQRLVLAATLSSVYGIHNGFELCENAAIPGKEEYADSEKY
DFKPRDWNAEGNIKDLIAKVNWIRNENPALQAFRTLRFFRADNDQILFYGKMTGDRANIILVAVNLDPHAGQGGLLWLPL
AEMGLSDGQPFIVEELLSGTRLEWTGSPHHYWFDPATNPAAIFRITP

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family. GlgE subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021828
- InterPro:   IPR006047
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF11896 DUF3416 [H]

EC number: 3.2.1.1

Molecular weight: Translated: 76411; Mature: 76279

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSALPPFRPSLVSDPLSARQDPSLPGILPVSAPILIEDIGPRVSDGRYPIKRVLGEGVV
CCCCCCCCCCHHHCCCHHCCCCCCCCCEECCCCCEEEECCCCCCCCCCCHHHHHHCCCEE
VSAAVFRDGHVPLAAALLWRRSPRPGEPAGGRGAWHEVPLTQIEPGLARWRARFDPPAIG
EEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHCCCCCCCC
RFDYLIEAWSDDWAGWRHDTLIKRDAGLDLSLEVLEGMALIEATIARIDGTDQAADQGLL
HHHHHHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSWLADAQGLETAGGRATLMLSETLDAVMARHPDRSGAVRSGPPLSVVVDPPRAGFAAWY
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
EMAPRSQASEPGRWGTFADCAARLPDIRAMGFDTLYLMPIHPIGRIHRKGPNNSLKAGPD
HHCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCEEEEEECCCHHHHHCCCCCCCCCCCCC
DPGSPYAIGAAEGGHTAIHPELGTLDEFRALVAQAGEMGMDVALDIAIQCAPDHPWVSEH
CCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCC
PEWFEFRPDGTIKYAENPPKKYQDIVNLAFWGPHRQELWLALLEVFTFWAEQGVRVFRVD
CCCEEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEC
NPHTKPVPFWEWLIATVKARYPDCIFLAEAFTRPPMMKMLAKIGFTQSYSYFTWRTTKAE
CCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHH
LTSYMEELVEDDPADYMRVNFFANTPDILPFHLQTGGRPAFMQRLVLAATLSSVYGIHNG
HHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
FELCENAAIPGKEEYADSEKYDFKPRDWNAEGNIKDLIAKVNWIRNENPALQAFRTLRFF
HHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
RADNDQILFYGKMTGDRANIILVAVNLDPHAGQGGLLWLPLAEMGLSDGQPFIVEELLSG
CCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHCC
TRLEWTGSPHHYWFDPATNPAAIFRITP
CEEEECCCCCCEEECCCCCCEEEEEECC
>Mature Secondary Structure 
SSALPPFRPSLVSDPLSARQDPSLPGILPVSAPILIEDIGPRVSDGRYPIKRVLGEGVV
CCCCCCCCCHHHCCCHHCCCCCCCCCEECCCCCEEEECCCCCCCCCCCHHHHHHCCCEE
VSAAVFRDGHVPLAAALLWRRSPRPGEPAGGRGAWHEVPLTQIEPGLARWRARFDPPAIG
EEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHCCCCCCCC
RFDYLIEAWSDDWAGWRHDTLIKRDAGLDLSLEVLEGMALIEATIARIDGTDQAADQGLL
HHHHHHHHHCCCCCCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
SSWLADAQGLETAGGRATLMLSETLDAVMARHPDRSGAVRSGPPLSVVVDPPRAGFAAWY
HHHHHHHCCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHH
EMAPRSQASEPGRWGTFADCAARLPDIRAMGFDTLYLMPIHPIGRIHRKGPNNSLKAGPD
HHCCCCCCCCCCCCCCHHHHHHCCCCCEECCCCEEEEEECCCHHHHHCCCCCCCCCCCCC
DPGSPYAIGAAEGGHTAIHPELGTLDEFRALVAQAGEMGMDVALDIAIQCAPDHPWVSEH
CCCCCEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCC
PEWFEFRPDGTIKYAENPPKKYQDIVNLAFWGPHRQELWLALLEVFTFWAEQGVRVFRVD
CCCEEECCCCCEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEC
NPHTKPVPFWEWLIATVKARYPDCIFLAEAFTRPPMMKMLAKIGFTQSYSYFTWRTTKAE
CCCCCCCCHHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHCCCCCCCEEEEECCHHH
LTSYMEELVEDDPADYMRVNFFANTPDILPFHLQTGGRPAFMQRLVLAATLSSVYGIHNG
HHHHHHHHHCCCCCCEEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCH
FELCENAAIPGKEEYADSEKYDFKPRDWNAEGNIKDLIAKVNWIRNENPALQAFRTLRFF
HHHHHCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHH
RADNDQILFYGKMTGDRANIILVAVNLDPHAGQGGLLWLPLAEMGLSDGQPFIVEELLSG
CCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCEEEEEHHHCCCCCCCCHHHHHHHCC
TRLEWTGSPHHYWFDPATNPAAIFRITP
CEEEECCCCCCEEECCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; cholesteryl-beta-D-glucoside

Specific reaction: H2O + cholesteryl-beta-D-glucoside = D-glucose + cholesterol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10970853 [H]