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The map label for this gene is proS

Identifier: 83592911

GI number: 83592911

Start: 1856768

End: 1858075

Strand: Direct

Name: proS

Synonym: Rru_A1576

Alternate gene names: 83592911

Gene position: 1856768-1858075 (Clockwise)

Preceding gene: 83592910

Following gene: 83592912

Centisome position: 42.66

GC content: 63.69

Gene sequence:

>1308_bases
ATGCGCCTGTCCCGCTTTTTGCTGCCCACCCTCAAGGAAACCCCTTCCGAGGCGGAAATCGTCTCCCATCGGCTGATGCT
GCGCGCCGGCATGATCCGCCAGCACGCCTCGGGCATCTATAACTGGCTGCCGCTGGGTCTGCGCGTGCTGCGCAAGATCG
AACAGGTGGTGCGCGAGGAACAGGATGCGACCGGCGCCCAGGAAATCCTGATGCCGACCATCCAGTCGGCCGATCTGTGG
CGCGAAAGCGGGCGCTATGACGATTACGGCAAGGAAATGCTGCGCATCGTCGACCGCCACGAGCGCGACATGCTCTATGG
CCCCACCCACGAAGAGGTGGCGACCGATGTCTTCCGCAAGAATGTCAAAAGCTACCGCGCCCTGCCGCAGAACCTCTATC
AGATCCAGTGGAAGTTCCGCGACGAGGTGCGCCCGCGCTTTGGCGTGATGCGCGGCCGCGAATTCCTGATGAAGGACAAT
TACTCCTTCGATCTGACCTATGAGGGCGCCCGGCACTCCTACAACAAGATGTTCGTGGCCTATCTGCGCACCTTCGCCCG
CCTGGGGCTGAAGGCGATCCCGATGGCGGCCGATACCGGCCCGATCGGCGGCAAGCTCAGCCATGAATTCATCATCCTGG
CCGATACGGGCGAAAGCGCCGTGTTCTGCCATCGCGACCTGCTCGACAAGCCCGCCCCCGAGAATGTCGATTACGACTCC
GACCTTCAGCCGCTGGTCGATTCCTGGACCAGCCTCTATGCCGCCACCGACGAGATGCACCGTCCCGACCATGGCGTGCC
CGAAGGCGATCTGGTCAGCGCCCGGGGTATCGAGGTCGGCCATATCTTCCATTTCGGCACCAAATATTCGGCGCCGATGG
GCGCCACCGTCACCGCGCCCGATGGCTCGAGCCAAGCGGTGTTCATGGGGTCTTACGGCATCGGCGTGTCGCGTCTGGTC
GCCGGCATCATCGAAGCCAGCCACGATGACAACGGCATCATCTGGCCCGATGGTGTCGCCCCCTTCGATATCGGCGTGAT
CAACCTCAAGGTCGGCGACGCCGCCACCGATGGCGTCTGCGCCGATCTTTATGGCCGTCTGCGCGCGGCCGGCAAGGACG
TGCTGTTTGACGATACCGACGACCGCGCCGGCGCCAAATTCGCCACCATGGATCTGATCGGCCTGCCCTGGCAGGTGATC
GCCGGACCCAAGGGCGTGGCCAAGGGCATGGTCGAGTTGAAGGAACGGGCCACCGGCGAACGCCACGAACTGTCGATCGA
CAGCGCCCTGGCCAAGCTTCTCGGCTGA

Upstream 100 bases:

>100_bases
ATGCTTTGAGCGCCTTGGAAACCGCACGATCAAGGACTAGAGTGCGCCTTCCTTTCGCCCCGCCGTTTTTGTGACGACCC
CTCTCGGATAGAAATCAACC

Downstream 100 bases:

>100_bases
TCGTTCAAGGGGCGCGGCGCGCGACACCGGCGAGCCCGGTTTTTCGAAAGTGTGCCGCCCCCTGTTTGCGACTTGTGGAT
CAAACCAACGGGACGAAGGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MRLSRFLLPTLKETPSEAEIVSHRLMLRAGMIRQHASGIYNWLPLGLRVLRKIEQVVREEQDATGAQEILMPTIQSADLW
RESGRYDDYGKEMLRIVDRHERDMLYGPTHEEVATDVFRKNVKSYRALPQNLYQIQWKFRDEVRPRFGVMRGREFLMKDN
YSFDLTYEGARHSYNKMFVAYLRTFARLGLKAIPMAADTGPIGGKLSHEFIILADTGESAVFCHRDLLDKPAPENVDYDS
DLQPLVDSWTSLYAATDEMHRPDHGVPEGDLVSARGIEVGHIFHFGTKYSAPMGATVTAPDGSSQAVFMGSYGIGVSRLV
AGIIEASHDDNGIIWPDGVAPFDIGVINLKVGDAATDGVCADLYGRLRAAGKDVLFDDTDDRAGAKFATMDLIGLPWQVI
AGPKGVAKGMVELKERATGERHELSIDSALAKLLG

Sequences:

>Translated_435_residues
MRLSRFLLPTLKETPSEAEIVSHRLMLRAGMIRQHASGIYNWLPLGLRVLRKIEQVVREEQDATGAQEILMPTIQSADLW
RESGRYDDYGKEMLRIVDRHERDMLYGPTHEEVATDVFRKNVKSYRALPQNLYQIQWKFRDEVRPRFGVMRGREFLMKDN
YSFDLTYEGARHSYNKMFVAYLRTFARLGLKAIPMAADTGPIGGKLSHEFIILADTGESAVFCHRDLLDKPAPENVDYDS
DLQPLVDSWTSLYAATDEMHRPDHGVPEGDLVSARGIEVGHIFHFGTKYSAPMGATVTAPDGSSQAVFMGSYGIGVSRLV
AGIIEASHDDNGIIWPDGVAPFDIGVINLKVGDAATDGVCADLYGRLRAAGKDVLFDDTDDRAGAKFATMDLIGLPWQVI
AGPKGVAKGMVELKERATGERHELSIDSALAKLLG
>Mature_435_residues
MRLSRFLLPTLKETPSEAEIVSHRLMLRAGMIRQHASGIYNWLPLGLRVLRKIEQVVREEQDATGAQEILMPTIQSADLW
RESGRYDDYGKEMLRIVDRHERDMLYGPTHEEVATDVFRKNVKSYRALPQNLYQIQWKFRDEVRPRFGVMRGREFLMKDN
YSFDLTYEGARHSYNKMFVAYLRTFARLGLKAIPMAADTGPIGGKLSHEFIILADTGESAVFCHRDLLDKPAPENVDYDS
DLQPLVDSWTSLYAATDEMHRPDHGVPEGDLVSARGIEVGHIFHFGTKYSAPMGATVTAPDGSSQAVFMGSYGIGVSRLV
AGIIEASHDDNGIIWPDGVAPFDIGVINLKVGDAATDGVCADLYGRLRAAGKDVLFDDTDDRAGAKFATMDLIGLPWQVI
AGPKGVAKGMVELKERATGERHELSIDSALAKLLG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 2 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=431, Percent_Identity=35.0348027842227, Blast_Score=255, Evalue=5e-68,
Organism=Escherichia coli, GI1786392, Length=221, Percent_Identity=60.1809954751131, Blast_Score=288, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI115532348, Length=400, Percent_Identity=29.75, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI193203271, Length=91, Percent_Identity=32.967032967033, Blast_Score=68, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6320931, Length=203, Percent_Identity=35.9605911330049, Blast_Score=146, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24656200, Length=447, Percent_Identity=30.6487695749441, Blast_Score=216, Evalue=4e-56,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_RHORT (Q2RU19)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426663.1
- ProteinModelPortal:   Q2RU19
- SMR:   Q2RU19
- STRING:   Q2RU19
- GeneID:   3834993
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1576
- NMPDR:   fig|1085.1.peg.2791
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q2RU19
- ProtClustDB:   PRK12325
- BioCyc:   RRUB269796:RRU_A1576-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01570
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.15

Molecular weight: Translated: 48373; Mature: 48373

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSRFLLPTLKETPSEAEIVSHRLMLRAGMIRQHASGIYNWLPLGLRVLRKIEQVVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDATGAQEILMPTIQSADLWRESGRYDDYGKEMLRIVDRHERDMLYGPTHEEVATDVFRK
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
NVKSYRALPQNLYQIQWKFRDEVRPRFGVMRGREFLMKDNYSFDLTYEGARHSYNKMFVA
HHHHHHHHHHHHHHEEEHHHHHCCHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHH
YLRTFARLGLKAIPMAADTGPIGGKLSHEFIILADTGESAVFCHRDLLDKPAPENVDYDS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHCCCCCCCCCCCC
DLQPLVDSWTSLYAATDEMHRPDHGVPEGDLVSARGIEVGHIFHFGTKYSAPMGATVTAP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCEEECC
DGSSQAVFMGSYGIGVSRLVAGIIEASHDDNGIIWPDGVAPFDIGVINLKVGDAATDGVC
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEEECCCCCCCHH
ADLYGRLRAAGKDVLFDDTDDRAGAKFATMDLIGLPWQVIAGPKGVAKGMVELKERATGE
HHHHHHHHHCCCCCEECCCCCCCCCEEEEEHHHCCCHHEECCCHHHHHHHHHHHHHCCCC
RHELSIDSALAKLLG
HHHHHHHHHHHHHHC
>Mature Secondary Structure
MRLSRFLLPTLKETPSEAEIVSHRLMLRAGMIRQHASGIYNWLPLGLRVLRKIEQVVREE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDATGAQEILMPTIQSADLWRESGRYDDYGKEMLRIVDRHERDMLYGPTHEEVATDVFRK
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
NVKSYRALPQNLYQIQWKFRDEVRPRFGVMRGREFLMKDNYSFDLTYEGARHSYNKMFVA
HHHHHHHHHHHHHHEEEHHHHHCCHHHHHHHCCHHHCCCCCEEEEEECCCHHHHHHHHHH
YLRTFARLGLKAIPMAADTGPIGGKLSHEFIILADTGESAVFCHRDLLDKPAPENVDYDS
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEHHHHCCCCCCCCCCCC
DLQPLVDSWTSLYAATDEMHRPDHGVPEGDLVSARGIEVGHIFHFGTKYSAPMGATVTAP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCCCCCEEECC
DGSSQAVFMGSYGIGVSRLVAGIIEASHDDNGIIWPDGVAPFDIGVINLKVGDAATDGVC
CCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCCCCCCEEEEEEEEECCCCCCCHH
ADLYGRLRAAGKDVLFDDTDDRAGAKFATMDLIGLPWQVIAGPKGVAKGMVELKERATGE
HHHHHHHHHCCCCCEECCCCCCCCCEEEEEHHHCCCHHEECCCHHHHHHHHHHHHHCCCC
RHELSIDSALAKLLG
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA