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The map label for this gene is vapI [H]

Identifier: 83592870

GI number: 83592870

Start: 1808530

End: 1808901

Strand: Reverse

Name: vapI [H]

Synonym: Rru_A1535

Alternate gene names: 83592870

Gene position: 1808901-1808530 (Counterclockwise)

Preceding gene: 83592872

Following gene: 83592867

Centisome position: 41.56

GC content: 63.98

Gene sequence:

>372_bases
ATGTCCGCGCCGGACGACAAACCACATCATCCGGGGGAAACCCTGGCGCGGCGGTTCATGGAGCCCAACCGCCTTGGCAT
CAATCAGCTTGCCCGGGCCCTGGCGGTGCCGCCCAATCGCATCAGCGCCATTTTATCGGGGCAGCGCTCGATCACCGCCG
ATACGGCCTTGCGGCTGGCCCGGTTTTTCGACACCACAGCGCTTTATTGGATGAACCTGCAGGCGCGCTATGATCTGGCG
CTGACCCAGCAGGTCCTGGGCGAGGAATTGCGTACCACGCTGTCCACCCTGAAGGACCACCAACGCACGCCTTTGGATAA
TTCCCCGGTGGCCGAAACCGCCCCCCCGCCCCTTTCCGAACAGGCGCCTTAG

Upstream 100 bases:

>100_bases
GGCCCGTCGCGCCACCGATTGCGCCGGGAAGACCGCAAGCGAGCTTGCGCGAAAGGCCGGCTCGGCTATTCTACCCCTAC
CTTAGGAACCGGAGGAGCCG

Downstream 100 bases:

>100_bases
CAAATCCCGGGCACTCTGGACCAAAGCGCGACGACGATTTGCGCCCCTCTTCTGCCGATGCCGGTTTATCGGTCGCCCTC
CGCCTCGTCGAGGGCCCGCA

Product: plasmid maintenance system antidote protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MSAPDDKPHHPGETLARRFMEPNRLGINQLARALAVPPNRISAILSGQRSITADTALRLARFFDTTALYWMNLQARYDLA
LTQQVLGEELRTTLSTLKDHQRTPLDNSPVAETAPPPLSEQAP

Sequences:

>Translated_123_residues
MSAPDDKPHHPGETLARRFMEPNRLGINQLARALAVPPNRISAILSGQRSITADTALRLARFFDTTALYWMNLQARYDLA
LTQQVLGEELRTTLSTLKDHQRTPLDNSPVAETAPPPLSEQAP
>Mature_122_residues
SAPDDKPHHPGETLARRFMEPNRLGINQLARALAVPPNRISAILSGQRSITADTALRLARFFDTTALYWMNLQARYDLAL
TQQVLGEELRTTLSTLKDHQRTPLDNSPVAETAPPPLSEQAP

Specific function: Unknown

COG id: COG3093

COG function: function code R; Plasmid maintenance system antidote protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081745, Length=79, Percent_Identity=44.3037974683544, Blast_Score=73, Evalue=6e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013430
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 13655; Mature: 13523

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPDDKPHHPGETLARRFMEPNRLGINQLARALAVPPNRISAILSGQRSITADTALRLA
CCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHH
RFFDTTALYWMNLQARYDLALTQQVLGEELRTTLSTLKDHQRTPLDNSPVAETAPPPLSE
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
QAP
CCC
>Mature Secondary Structure 
SAPDDKPHHPGETLARRFMEPNRLGINQLARALAVPPNRISAILSGQRSITADTALRLA
CCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHH
RFFDTTALYWMNLQARYDLALTQQVLGEELRTTLSTLKDHQRTPLDNSPVAETAPPPLSE
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
QAP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8169216 [H]