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The map label for this gene is ctfB [H]

Identifier: 83592807

GI number: 83592807

Start: 1737584

End: 1738243

Strand: Reverse

Name: ctfB [H]

Synonym: Rru_A1471

Alternate gene names: 83592807

Gene position: 1738243-1737584 (Counterclockwise)

Preceding gene: 83592808

Following gene: 83592806

Centisome position: 39.93

GC content: 65.3

Gene sequence:

>660_bases
ATGGATGACAAGACCCTTATCGCCAAGCGCGTCGCCCTGGAACTGCGGCCCGGGGATCTCGCCAATCTCGGCATCGGCCT
GCCAACCCTGGTCGCCTCCTATCTGACCCCGGGCGTCGAGGTGTTCTTCCAGTCGGAGAACGGCATCGTCGGCATGAGCC
CGCTGCCCGATATCGGTTTCGCCGATGACGATCTGACCGATGCCGGCGGCAGTCCGATCGGTGTTCTGCCCGGCGCCTGC
GCTTTTGATTCGGCCTTTTCCTTCGGGTTGATCCGCGGCGGACATCTTGATGTCACCGTGCTCGGCGGCCTGCAGATCGA
CGAGCGCGGCCTGCTCGCCAACTGGATGGTGCCGGGCAAGATGGTGCCGGGCATGGGGGGGGCGATGGATCTGGTCTCGG
GCGCCAAGCGCGTCATCGTCGCCATGACCCATACGGCCAAGGGCGCGCCCAAGATCGTCAAGACCTGCACCCTGCCGCTG
ACCTCGGTTCGTCGGGTCGATCTGGTGGTGACGGAGCTCGCCGTGATCGAGCCCACCGCCAAGGGCCTGCTGCTGCGCGA
AGTCGCCCCCGGCGTTTCGGTCGACCAGGTGATCGAAGCCACCGAGGCCAACTTGCTGATCGCCGAGGATCTTCGCGAGA
TGCCCATTCTTGCCGCTTAA

Upstream 100 bases:

>100_bases
GCCGAGGCCGAGGACTTCGTGCCCATCGGCGCCATCGAACCCGACGCGGTGGCGACCCCGCATGTTCTGGTCGATGTCCT
TATCGCCAAGGAGCGGTCCC

Downstream 100 bases:

>100_bases
CGCGCCCCAAAGGGCGCCTTGCTTACGCGCCCTAACGGCGCCTTGCGCTTAAAGACAGGACCGGAGTTTTTTCATGCCCA
TTCCCGCCAATGACGCCCTG

Product: butyryl-CoA:acetate CoA transferase

Products: NA

Alternate protein names: Coat B; Acetoacetyl-CoA:acetate/butyrate:CoA transferase subunit B [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MDDKTLIAKRVALELRPGDLANLGIGLPTLVASYLTPGVEVFFQSENGIVGMSPLPDIGFADDDLTDAGGSPIGVLPGAC
AFDSAFSFGLIRGGHLDVTVLGGLQIDERGLLANWMVPGKMVPGMGGAMDLVSGAKRVIVAMTHTAKGAPKIVKTCTLPL
TSVRRVDLVVTELAVIEPTAKGLLLREVAPGVSVDQVIEATEANLLIAEDLREMPILAA

Sequences:

>Translated_219_residues
MDDKTLIAKRVALELRPGDLANLGIGLPTLVASYLTPGVEVFFQSENGIVGMSPLPDIGFADDDLTDAGGSPIGVLPGAC
AFDSAFSFGLIRGGHLDVTVLGGLQIDERGLLANWMVPGKMVPGMGGAMDLVSGAKRVIVAMTHTAKGAPKIVKTCTLPL
TSVRRVDLVVTELAVIEPTAKGLLLREVAPGVSVDQVIEATEANLLIAEDLREMPILAA
>Mature_219_residues
MDDKTLIAKRVALELRPGDLANLGIGLPTLVASYLTPGVEVFFQSENGIVGMSPLPDIGFADDDLTDAGGSPIGVLPGAC
AFDSAFSFGLIRGGHLDVTVLGGLQIDERGLLANWMVPGKMVPGMGGAMDLVSGAKRVIVAMTHTAKGAPKIVKTCTLPL
TSVRRVDLVVTELAVIEPTAKGLLLREVAPGVSVDQVIEATEANLLIAEDLREMPILAA

Specific function: Involved in solventogenic switch which allows C.acetobutylicum to uptake acid and produce solvents. Acts mainly to detoxify the medium by removing the acetate and butyrate excreted earlier in the fermentation [H]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=220, Percent_Identity=48.6363636363636, Blast_Score=201, Evalue=3e-52,
Organism=Homo sapiens, GI11545841, Length=220, Percent_Identity=47.2727272727273, Blast_Score=199, Evalue=2e-51,
Organism=Escherichia coli, GI1788552, Length=213, Percent_Identity=56.8075117370892, Blast_Score=234, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17531517, Length=215, Percent_Identity=48.8372093023256, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=47.6635514018692, Blast_Score=198, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=47.6635514018692, Blast_Score=198, Evalue=3e-51,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.9 [H]

Molecular weight: Translated: 22751; Mature: 22751

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDKTLIAKRVALELRPGDLANLGIGLPTLVASYLTPGVEVFFQSENGIVGMSPLPDIGF
CCCHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCC
ADDDLTDAGGSPIGVLPGACAFDSAFSFGLIRGGHLDVTVLGGLQIDERGLLANWMVPGK
CCCCCCCCCCCCCCCCCCCHHHHCHHHHHEEECCCEEEEEECCEEECCCCCEEEECCCCC
MVPGMGGAMDLVSGAKRVIVAMTHTAKGAPKIVKTCTLPLTSVRRVDLVVTELAVIEPTA
CCCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHHEECCCHHHHHHHHHHHHHHHEECCCH
KGLLLREVAPGVSVDQVIEATEANLLIAEDLREMPILAA
HCEEEEHHCCCCCHHHHHHHHCCCEEEEHHHHHCCCCCC
>Mature Secondary Structure
MDDKTLIAKRVALELRPGDLANLGIGLPTLVASYLTPGVEVFFQSENGIVGMSPLPDIGF
CCCHHHEEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCC
ADDDLTDAGGSPIGVLPGACAFDSAFSFGLIRGGHLDVTVLGGLQIDERGLLANWMVPGK
CCCCCCCCCCCCCCCCCCCHHHHCHHHHHEEECCCEEEEEECCEEECCCCCEEEECCCCC
MVPGMGGAMDLVSGAKRVIVAMTHTAKGAPKIVKTCTLPLTSVRRVDLVVTELAVIEPTA
CCCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHHEECCCHHHHHHHHHHHHHHHEECCCH
KGLLLREVAPGVSVDQVIEATEANLLIAEDLREMPILAA
HCEEEEHHCCCCCHHHHHHHHCCCEEEEHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254264; 8226639; 8423010; 11466286; 2383002 [H]