Click here to switch to the map view.

The map label for this gene is dctA

Identifier: 83592773

GI number: 83592773

Start: 1699627

End: 1700970

Strand: Direct

Name: dctA

Synonym: Rru_A1437

Alternate gene names: 83592773

Gene position: 1699627-1700970 (Clockwise)

Preceding gene: 83592767

Following gene: 83592774

Centisome position: 39.05

GC content: 62.43

Gene sequence:

>1344_bases
ATGGCCTTCGTTGTGCCCGCGGCGCCGCCGCGCACACCCCATAAGTTCTATCAGATCCTTTATGTTCAGGTTTTGGTCGC
CATCGTCGCCGGCGTGTTGCTTGGGCATTTCTACCCCGATTTGGGGTCGGCGCTGAAGCCGCTGGGCGATGCGTTCATCA
AGCTTGTGAAGATGATCATCGCGCCGGTGATCTTCCTGACCGTTGTCACCGGCATCGCCGGCCTGACCGATATGCAAAAG
GTCGGCCGGGTCGCCGGCAAGGCGATGATCTACTTCCTGTCGTTTTCGACCCTGGCCCTGATCATCGGCATGATCGTCGC
CAATGTCGTTCATCCGGGATCGGGCCTCAACATCGACCCGGCCTCGCTCGACGCCAAGGCGGTGCAGACCTATACCAGCC
ACGCCCATGACCAGTCGCTGGTCGGCTTCCTGATGAACATCATCCCGTCAACGCCGATCAGCGCCTTCGCCTCGGGCGAT
ATCTTGCAGGTGCTGTTCTTCGCCGTGCTGTTTGGCATCGCCCTGGCCACCGTCGGCGAACGCGGCAAGCCGGTGCTTGA
CCTGCTCAATCCGCTTTTGCAGGTGGTCTTCCGGCTGGTCGCCATCGTCATGAAGGCCGCCCCGCTGGGCGCTTTCGGCG
CCATGGCCTTCACCATCGGCAAATACGGCATCGGCTCGATCGCCAATCTGGCGATGCTGGTCGGCACCTTCTATCTGACG
TCCGGGCTGTTCGTTTTTGTCGTTCTGGGCCTCGTCGCCCGCTACAACGGCTTCTCGTTGTTGGCCCTGCTGCGCTACAT
CAAGGAAGAGCTGCTGCTGGTCCTCGGTACCAGCTCCTCCGAGGCGGCCCTGCCGACCCTGATGGAAAAGCTCGAGCGGG
CGGGCTGCAAGAAGTCGGTGGTCGGGCTGGTGGTGCCCACGGGATATTCCTTCAATCTTGATGGCACCAATATCTATATG
ACCCTGGCGGCGCTGTTCATCGCCCAGGCGACCAACATCGATCTCAGCTTGCAAGACCAGATCCTGCTGCTGCTGGTGGC
GATGCTCAGCTCCAAGGGCGCGGCCGGCATCACCGGCGCCGGCTTCATCACCCTGGCCGCCACCTTGTCGGTGGTGCCTT
CGGTGCCGGTGGCCGGCATGGCGCTGATCCTCGGCGTCGACCGTTTCATGTCGGAATGCCGGGCCCTGACCAATTTCATT
GGCAATGCGGTGGCGACCATCGTCGTTGCCAAGTGGGAAGGCGAACTCGACCGCGACCGGTTGGCCCTGGCGCTGAAGGG
CGGCGGCAAGGCGCTTTTGCCGCTCGAACACGAGGCGGCGATCCACATCCCCTCCAAAGTGTGA

Upstream 100 bases:

>100_bases
TCAAGGAGGGGTCGCAGCCGCGATCCCGATCACCCCAGCAGCCTCGGATCCAACGGCGTAACCCCCCAACAAGCGCCGAC
GTCCGTGGGAGGAGATTACC

Downstream 100 bases:

>100_bases
CTCTGGGTTAAGGAGTCGCGTCGCGCCGCCGTGGGGATGCCCTCTCCACGGCGGCGTTTTCATAAGGGGCCCCGCCCAAA
GGTTTGCGAATAATTCTCAA

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MAFVVPAAPPRTPHKFYQILYVQVLVAIVAGVLLGHFYPDLGSALKPLGDAFIKLVKMIIAPVIFLTVVTGIAGLTDMQK
VGRVAGKAMIYFLSFSTLALIIGMIVANVVHPGSGLNIDPASLDAKAVQTYTSHAHDQSLVGFLMNIIPSTPISAFASGD
ILQVLFFAVLFGIALATVGERGKPVLDLLNPLLQVVFRLVAIVMKAAPLGAFGAMAFTIGKYGIGSIANLAMLVGTFYLT
SGLFVFVVLGLVARYNGFSLLALLRYIKEELLLVLGTSSSEAALPTLMEKLERAGCKKSVVGLVVPTGYSFNLDGTNIYM
TLAALFIAQATNIDLSLQDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNFI
GNAVATIVVAKWEGELDRDRLALALKGGGKALLPLEHEAAIHIPSKV

Sequences:

>Translated_447_residues
MAFVVPAAPPRTPHKFYQILYVQVLVAIVAGVLLGHFYPDLGSALKPLGDAFIKLVKMIIAPVIFLTVVTGIAGLTDMQK
VGRVAGKAMIYFLSFSTLALIIGMIVANVVHPGSGLNIDPASLDAKAVQTYTSHAHDQSLVGFLMNIIPSTPISAFASGD
ILQVLFFAVLFGIALATVGERGKPVLDLLNPLLQVVFRLVAIVMKAAPLGAFGAMAFTIGKYGIGSIANLAMLVGTFYLT
SGLFVFVVLGLVARYNGFSLLALLRYIKEELLLVLGTSSSEAALPTLMEKLERAGCKKSVVGLVVPTGYSFNLDGTNIYM
TLAALFIAQATNIDLSLQDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNFI
GNAVATIVVAKWEGELDRDRLALALKGGGKALLPLEHEAAIHIPSKV
>Mature_446_residues
AFVVPAAPPRTPHKFYQILYVQVLVAIVAGVLLGHFYPDLGSALKPLGDAFIKLVKMIIAPVIFLTVVTGIAGLTDMQKV
GRVAGKAMIYFLSFSTLALIIGMIVANVVHPGSGLNIDPASLDAKAVQTYTSHAHDQSLVGFLMNIIPSTPISAFASGDI
LQVLFFAVLFGIALATVGERGKPVLDLLNPLLQVVFRLVAIVMKAAPLGAFGAMAFTIGKYGIGSIANLAMLVGTFYLTS
GLFVFVVLGLVARYNGFSLLALLRYIKEELLLVLGTSSSEAALPTLMEKLERAGCKKSVVGLVVPTGYSFNLDGTNIYMT
LAALFIAQATNIDLSLQDQILLLLVAMLSSKGAAGITGAGFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNFIG
NAVATIVVAKWEGELDRDRLALALKGGGKALLPLEHEAAIHIPSKV

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI40254478, Length=440, Percent_Identity=29.0909090909091, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI169790839, Length=417, Percent_Identity=28.0575539568345, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI194239697, Length=487, Percent_Identity=25.4620123203285, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4827012, Length=294, Percent_Identity=30.952380952381, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI66773030, Length=470, Percent_Identity=26.1702127659574, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI21314632, Length=404, Percent_Identity=26.980198019802, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI5032093, Length=304, Percent_Identity=30.2631578947368, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI223468566, Length=290, Percent_Identity=30, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI262359914, Length=372, Percent_Identity=26.6129032258064, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI223468564, Length=260, Percent_Identity=31.9230769230769, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI301601644, Length=167, Percent_Identity=30.5389221556886, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1789947, Length=405, Percent_Identity=61.7283950617284, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1790514, Length=410, Percent_Identity=40, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1788024, Length=387, Percent_Identity=24.8062015503876, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789473, Length=320, Percent_Identity=24.375, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17537407, Length=437, Percent_Identity=27.0022883295195, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71983099, Length=455, Percent_Identity=26.1538461538462, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=455, Percent_Identity=26.1538461538462, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=459, Percent_Identity=26.797385620915, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71996953, Length=441, Percent_Identity=27.2108843537415, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI193206654, Length=433, Percent_Identity=23.7875288683603, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=453, Percent_Identity=24.2825607064018, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24583025, Length=415, Percent_Identity=25.7831325301205, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17137668, Length=415, Percent_Identity=25.7831325301205, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583023, Length=415, Percent_Identity=25.7831325301205, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI281360481, Length=467, Percent_Identity=25.2676659528908, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI281360483, Length=467, Percent_Identity=25.2676659528908, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17137666, Length=467, Percent_Identity=25.2676659528908, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_RHORT (Q2RUF7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426525.1
- ProteinModelPortal:   Q2RUF7
- SMR:   Q2RUF7
- STRING:   Q2RUF7
- GeneID:   3834852
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1437
- NMPDR:   fig|1085.1.peg.2930
- eggNOG:   COG1301
- HOGENOM:   HBG739804
- OMA:   AGCKRSV
- PhylomeDB:   Q2RUF7
- ProtClustDB:   PRK01663
- BioCyc:   RRUB269796:RRU_A1437-MONOMER
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 47010; Mature: 46879

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xc30afc4)-; HASH(0xf5427f8)-; HASH(0xf293fdc)-; HASH(0xf3629a0)-; HASH(0xf55d9f0)-; HASH(0xdf4c3bc)-; HASH(0xeb529b4)-; HASH(0xefc7d80)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEEEECCCCEECEECCCCEECCCCCH
>Mature Secondary Structure 
AFVVPAAPPRTPHKFYQILYVQVLVAIVAGVLLGHFYPDLGSALKPLGDAFIKLVKMII
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
APVIFLTVVTGIAGLTDMQKVGRVAGKAMIYFLSFSTLALIIGMIVANVVHPGSGLNIDP
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ASLDAKAVQTYTSHAHDQSLVGFLMNIIPSTPISAFASGDILQVLFFAVLFGIALATVGE
CCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCC
RGKPVLDLLNPLLQVVFRLVAIVMKAAPLGAFGAMAFTIGKYGIGSIANLAMLVGTFYLT
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SGLFVFVVLGLVARYNGFSLLALLRYIKEELLLVLGTSSSEAALPTLMEKLERAGCKKSV
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHCCCHHCC
VGLVVPTGYSFNLDGTNIYMTLAALFIAQATNIDLSLQDQILLLLVAMLSSKGAAGITGA
EEEEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCH
GFITLAATLSVVPSVPVAGMALILGVDRFMSECRALTNFIGNAVATIVVAKWEGELDRDR
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
LALALKGGGKALLPLEHEAAIHIPSKV
EEEEEECCCCEECEECCCCEECCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA