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The map label for this gene is dctD [H]

Identifier: 83592771

GI number: 83592771

Start: 1696161

End: 1697498

Strand: Reverse

Name: dctD [H]

Synonym: Rru_A1435

Alternate gene names: 83592771

Gene position: 1697498-1696161 (Counterclockwise)

Preceding gene: 83592772

Following gene: 83592770

Centisome position: 39.0

GC content: 68.68

Gene sequence:

>1338_bases
ATGATCGACGTGGCGTTTATCGACGACGACGACACCATGCGCGCCGCCAATGTCCAGGCGCTGACCCTGGCCGGACTGAC
CGTCGCCCCCTTCGCCCTGCCCCACGAGGCCCTGGCGGCGATCGACGGCGCCTTTCCCGGCGTCGTCGTGACCGATGTAC
GCATGCCGCGCATCGACGGGCTGGAACTGTTCCGCCGGCTGCGCGCCATCGATCCCGATCTGCCGGTGATCCTGATCACC
GGCCACGGCGATATCGCCATGGCGGTCGATGCCATGCGCGAGGGCGCCTATGATTTCCTGGCCAAGCCCTATGCCGCCGA
CCGGCTGCTGACCAGCGTCCGCCACGCGCTGGACAAACGCCACCTGCAACTGGAAAACCGCGCCTTGCGCCGCGCCGCCG
AGGCGGCCAGCGAAAACCTGCCGCTGCTGGGCGACAGCCCGAGTATGGAGCGCCTGCGCAAGACCCTGCGCCAGATCGCC
GATGCCGATGTCGATGTGCTGGTCGAAGGCGAGACCGGCGCCGGCAAGGACGTGGTGGCCAATGCCCTCCACCGTTGGAG
CCGGCGCGCCGCCCGGCCCTTCGTCGCCGTGAACTGCGGCGCCCTGCCCGAAACGGTGATCGAAAGCGAACTTTTTGGCC
ATGAGGCCGGGGCCTTCACCGGCGCGGTGAAAAAACGCGTCGGCCGCATCGAGCACGCCCATGGCGGCACGCTGTTCCTT
GATGAGATCGAAGCCATGTCGCCGGGCCTGCAGGTCAAGCTGCTGCGCGTTCTGGAAACCCGCGAGATCGCGCCGCTGGG
CACCAACGACATCCGCCCCGTCGATATCCGCGTCGTCGCCGCCACCAAAACCGACCTGCCGACCCTGGTCCGCCAGGGGA
TGTTCCGCGAGGATCTCTATTATCGCCTGCATGTGGTGTCGCTGCGCGTGCCGCCTTTACGCGATCGCCGGGCCGATATC
GTGCCGCTGTTCGCCCATTTCCTCGACCGCGCCGCCAAGCAGTTCGGCCGCGCGGTGCCGGCGATCGGCGAGGCCGAACG
CCGCCATCTGCAAACCCACGACTGGCCGGGCAATGTCCGCGAACTGGCCCATTTCGCCAAACGCGTCGCGCTGGGCTTCA
GCGATGAGCCCGACCAAGCCGACCCGATCGGCCAGGACGAGCCGCTGCCCCGCCGGGTCGACGCCTTCGAAGCCGCCCAG
ATCCGCAAGGCCCTGGAAGCCACCCAGGGCGACGTGCAAGCCGCCTGCGAGGCCCTGGGGCTGCCCCGCAAAACCCTGTA
CGACAAGCTCCGCCGCCACGCCATCGACCAATCGGCCTATCGCCCGACCCCGGGGTGA

Upstream 100 bases:

>100_bases
TGATCGCCAAGGACATCATCGCCGAGTTCGGCGGCAGCTTCACCGGCGAGAACGCGGCAAGCGGCGGGGCGCGGTTCACC
ATTACCCTGGCGAGGCCCCA

Downstream 100 bases:

>100_bases
CAAAGTTCCCAACCGGCGCGAACCCCAAGCCTGCGCCAAGTCGCCGCCAGGATCGCGCTAACGCCATCCCCTTGATCGGC
CAACATTGTTTCCACCCCCT

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MIDVAFIDDDDTMRAANVQALTLAGLTVAPFALPHEALAAIDGAFPGVVVTDVRMPRIDGLELFRRLRAIDPDLPVILIT
GHGDIAMAVDAMREGAYDFLAKPYAADRLLTSVRHALDKRHLQLENRALRRAAEAASENLPLLGDSPSMERLRKTLRQIA
DADVDVLVEGETGAGKDVVANALHRWSRRAARPFVAVNCGALPETVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFL
DEIEAMSPGLQVKLLRVLETREIAPLGTNDIRPVDIRVVAATKTDLPTLVRQGMFREDLYYRLHVVSLRVPPLRDRRADI
VPLFAHFLDRAAKQFGRAVPAIGEAERRHLQTHDWPGNVRELAHFAKRVALGFSDEPDQADPIGQDEPLPRRVDAFEAAQ
IRKALEATQGDVQAACEALGLPRKTLYDKLRRHAIDQSAYRPTPG

Sequences:

>Translated_445_residues
MIDVAFIDDDDTMRAANVQALTLAGLTVAPFALPHEALAAIDGAFPGVVVTDVRMPRIDGLELFRRLRAIDPDLPVILIT
GHGDIAMAVDAMREGAYDFLAKPYAADRLLTSVRHALDKRHLQLENRALRRAAEAASENLPLLGDSPSMERLRKTLRQIA
DADVDVLVEGETGAGKDVVANALHRWSRRAARPFVAVNCGALPETVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFL
DEIEAMSPGLQVKLLRVLETREIAPLGTNDIRPVDIRVVAATKTDLPTLVRQGMFREDLYYRLHVVSLRVPPLRDRRADI
VPLFAHFLDRAAKQFGRAVPAIGEAERRHLQTHDWPGNVRELAHFAKRVALGFSDEPDQADPIGQDEPLPRRVDAFEAAQ
IRKALEATQGDVQAACEALGLPRKTLYDKLRRHAIDQSAYRPTPG
>Mature_445_residues
MIDVAFIDDDDTMRAANVQALTLAGLTVAPFALPHEALAAIDGAFPGVVVTDVRMPRIDGLELFRRLRAIDPDLPVILIT
GHGDIAMAVDAMREGAYDFLAKPYAADRLLTSVRHALDKRHLQLENRALRRAAEAASENLPLLGDSPSMERLRKTLRQIA
DADVDVLVEGETGAGKDVVANALHRWSRRAARPFVAVNCGALPETVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFL
DEIEAMSPGLQVKLLRVLETREIAPLGTNDIRPVDIRVVAATKTDLPTLVRQGMFREDLYYRLHVVSLRVPPLRDRRADI
VPLFAHFLDRAAKQFGRAVPAIGEAERRHLQTHDWPGNVRELAHFAKRVALGFSDEPDQADPIGQDEPLPRRVDAFEAAQ
IRKALEATQGDVQAACEALGLPRKTLYDKLRRHAIDQSAYRPTPG

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=445, Percent_Identity=39.3258426966292, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=38.2743362831858, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1790437, Length=444, Percent_Identity=39.1891891891892, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1790299, Length=463, Percent_Identity=37.3650107991361, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI1786524, Length=312, Percent_Identity=43.9102564102564, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87082117, Length=363, Percent_Identity=39.1184573002755, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=40.5970149253731, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=37.6175548589342, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI87082152, Length=233, Percent_Identity=47.2103004291845, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI87081872, Length=318, Percent_Identity=37.4213836477987, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1787583, Length=237, Percent_Identity=41.3502109704641, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87081858, Length=311, Percent_Identity=34.4051446945338, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=35.0393700787402, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48999; Mature: 48999

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVAFIDDDDTMRAANVQALTLAGLTVAPFALPHEALAAIDGAFPGVVVTDVRMPRIDG
CEEEEEECCCCCCHHCCCEEEEECCCCHHCHHCCHHHHHHHHCCCCCEEEECCCCCCCCH
LELFRRLRAIDPDLPVILITGHGDIAMAVDAMREGAYDFLAKPYAADRLLTSVRHALDKR
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHH
HLQLENRALRRAAEAASENLPLLGDSPSMERLRKTLRQIADADVDVLVEGETGAGKDVVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
NALHRWSRRAARPFVAVNCGALPETVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFL
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEE
DEIEAMSPGLQVKLLRVLETREIAPLGTNDIRPVDIRVVAATKTDLPTLVRQGMFREDLY
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHH
YRLHVVSLRVPPLRDRRADIVPLFAHFLDRAAKQFGRAVPAIGEAERRHLQTHDWPGNVR
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHH
ELAHFAKRVALGFSDEPDQADPIGQDEPLPRRVDAFEAAQIRKALEATQGDVQAACEALG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
LPRKTLYDKLRRHAIDQSAYRPTPG
CCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIDVAFIDDDDTMRAANVQALTLAGLTVAPFALPHEALAAIDGAFPGVVVTDVRMPRIDG
CEEEEEECCCCCCHHCCCEEEEECCCCHHCHHCCHHHHHHHHCCCCCEEEECCCCCCCCH
LELFRRLRAIDPDLPVILITGHGDIAMAVDAMREGAYDFLAKPYAADRLLTSVRHALDKR
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHH
HLQLENRALRRAAEAASENLPLLGDSPSMERLRKTLRQIADADVDVLVEGETGAGKDVVA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
NALHRWSRRAARPFVAVNCGALPETVIESELFGHEAGAFTGAVKKRVGRIEHAHGGTLFL
HHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCEEEE
DEIEAMSPGLQVKLLRVLETREIAPLGTNDIRPVDIRVVAATKTDLPTLVRQGMFREDLY
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHH
YRLHVVSLRVPPLRDRRADIVPLFAHFLDRAAKQFGRAVPAIGEAERRHLQTHDWPGNVR
EEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHH
ELAHFAKRVALGFSDEPDQADPIGQDEPLPRRVDAFEAAQIRKALEATQGDVQAACEALG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHC
LPRKTLYDKLRRHAIDQSAYRPTPG
CCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]