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The map label for this gene is ahpF [H]

Identifier: 83592753

GI number: 83592753

Start: 1673738

End: 1675318

Strand: Reverse

Name: ahpF [H]

Synonym: Rru_A1417

Alternate gene names: 83592753

Gene position: 1675318-1673738 (Counterclockwise)

Preceding gene: 83592754

Following gene: 83592749

Centisome position: 38.49

GC content: 64.39

Gene sequence:

>1581_bases
ATGTTGGACAGCAATCTCAAAGCCCAGGTGAAGACCTATCTCGAAAATGTCACCCAGCCGATCGAACTGGTCGCCTCGCT
CGACGAGAGCGCGAAGTCCGAAGAGATGCTTGACCTGCTCGAAGACATCGCCGGGCTTTCCGATAAGATCACCCTGATCG
GTCGCGACGACGAGGGCCGCAAGCCGTCGTTCTCGATCAACCGCGCCGGCACCGATATCGGCGTGCGCTTCGCCGGCCTG
CCGATGGGCCATGAATTCAATTCGTTGATCCTCGCCCTGCTCCACGTTGGCGGCCATCCGTCGAAGACGGCGCCCGAGGT
GGTCGAGCAGATTGAAAGCCTGGACGGCGACTATAATTTCGAAACCTATTTCTCGCTCTCGTGCCAGAACTGCCCCGATG
TGGTGCAGGCCCTCAATCTGATGAGCGCGCTCAACCCCCGCATCAAGCACACGGCCATCGATGGCGCCTTGTTCCAAGAC
GAGGTCGAAAGCCGTCAGGTCATGGCCGTGCCGGCCATTTACCTCAACGGCGAGCCCTTCGCCCAGGGCCGTATGACGCT
TGAACAGATCCTGGCCAAGCTTGACACCGGCGCGGCCGAGCGCACGGCGGCCAAGCTCAAGGACAAGGAGGCCTTCGACG
TTCTGGTCATCGGTGGCGGTCCGGCCGGCGCCGCGGCGGCGATCTATGCCGCGCGCAAGGGCATCCGCACCGGGGTGGCG
GCCGAACGCTTCGGCGGTCAGGTGCTCGATACCATGGGCATCGAGAACTTCATTTCCGTGGCCCATACCGAAGGCCCCAA
GCTGGCCGCCGCCCTGGAAAGCCACGTCAAGGACTACGCCGTCGACATCATGAACCTCCAGCGCGCCGAAAAGCTGATCC
CGGCCAAGACGCCCGGCGGTCTGGTCGAGGTGCAGTTGGCCAGCGGCGCGGCGCTGAAGGCCCGCACCGTCATCTTGTCA
ACCGGCGCCCGCTGGCGGCAGATGAATGTGCCGGGCGAGGATGCCTATCGCAACAAGGGCGTCGCCTATTGCCCGCATTG
CGACGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATCGGCGGCGGCAATTCGGGCGTGGAAGCCGCCATCGATCTGG
CCGGCATCGTCGCCCATGTCACCCTGATCGAATTCGACGCCCGCCTGCGCGCCGATCAGGTGTTGCAGACCAAGCTCGCC
AGCCTGAACAACGTGACGATCATCACCTCGGCCCTGACCAGCGAAGTCCACGGCGATGGCGCCAAGGTGGTCGGCCTCAG
CTACAAGGACCGCACCACCGAGGAATTCCACCGCATCGACCTTGACGGCATCTTCGTGCAGATCGGTCTGGTGCCCAATA
CGGAATGGCTGAAGGGCACGGTCGCCCTCAGCCCGCGCGGCGAAATCGAGATCGACGCCCGCGGCCAAACCTCGCTGCCC
GGCGTTTTCGCCGCCGGCGACGCCACCACGGTGCCCTATAAGCAGATCATCATCGCCATGGGCGAAGGCTCCAAGGCCGC
GCTGTCGGCCTTCGATTACCTGATCCGCCTGCCCCAGGCCGAAGAAACCGCCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCCCAGGGGGAGCGAGACGAAACAGGGTCAAACCATCATCCGGGCAGCGCACATCCTCGTTTCACGACATCAGGGGAGC
CCCAAGGGGAGTCAGCATTC

Downstream 100 bases:

>100_bases
CGCTCTCCTCCTGCTTGCCCGCAGCGAAACAGCCCGGTTCTTCCGGGCTGTTTTCGTGTGAAGACGGGCAACGCTCCCAT
CCCGCCTGCCGAAGTCGCCT

Product: thioredoxin-disulfide reductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MLDSNLKAQVKTYLENVTQPIELVASLDESAKSEEMLDLLEDIAGLSDKITLIGRDDEGRKPSFSINRAGTDIGVRFAGL
PMGHEFNSLILALLHVGGHPSKTAPEVVEQIESLDGDYNFETYFSLSCQNCPDVVQALNLMSALNPRIKHTAIDGALFQD
EVESRQVMAVPAIYLNGEPFAQGRMTLEQILAKLDTGAAERTAAKLKDKEAFDVLVIGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMGIENFISVAHTEGPKLAAALESHVKDYAVDIMNLQRAEKLIPAKTPGGLVEVQLASGAALKARTVILS
TGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDARLRADQVLQTKLA
SLNNVTIITSALTSEVHGDGAKVVGLSYKDRTTEEFHRIDLDGIFVQIGLVPNTEWLKGTVALSPRGEIEIDARGQTSLP
GVFAAGDATTVPYKQIIIAMGEGSKAALSAFDYLIRLPQAEETAAA

Sequences:

>Translated_526_residues
MLDSNLKAQVKTYLENVTQPIELVASLDESAKSEEMLDLLEDIAGLSDKITLIGRDDEGRKPSFSINRAGTDIGVRFAGL
PMGHEFNSLILALLHVGGHPSKTAPEVVEQIESLDGDYNFETYFSLSCQNCPDVVQALNLMSALNPRIKHTAIDGALFQD
EVESRQVMAVPAIYLNGEPFAQGRMTLEQILAKLDTGAAERTAAKLKDKEAFDVLVIGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMGIENFISVAHTEGPKLAAALESHVKDYAVDIMNLQRAEKLIPAKTPGGLVEVQLASGAALKARTVILS
TGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDARLRADQVLQTKLA
SLNNVTIITSALTSEVHGDGAKVVGLSYKDRTTEEFHRIDLDGIFVQIGLVPNTEWLKGTVALSPRGEIEIDARGQTSLP
GVFAAGDATTVPYKQIIIAMGEGSKAALSAFDYLIRLPQAEETAAA
>Mature_526_residues
MLDSNLKAQVKTYLENVTQPIELVASLDESAKSEEMLDLLEDIAGLSDKITLIGRDDEGRKPSFSINRAGTDIGVRFAGL
PMGHEFNSLILALLHVGGHPSKTAPEVVEQIESLDGDYNFETYFSLSCQNCPDVVQALNLMSALNPRIKHTAIDGALFQD
EVESRQVMAVPAIYLNGEPFAQGRMTLEQILAKLDTGAAERTAAKLKDKEAFDVLVIGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMGIENFISVAHTEGPKLAAALESHVKDYAVDIMNLQRAEKLIPAKTPGGLVEVQLASGAALKARTVILS
TGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDARLRADQVLQTKLA
SLNNVTIITSALTSEVHGDGAKVVGLSYKDRTTEEFHRIDLDGIFVQIGLVPNTEWLKGTVALSPRGEIEIDARGQTSLP
GVFAAGDATTVPYKQIIIAMGEGSKAALSAFDYLIRLPQAEETAAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=523, Percent_Identity=65.774378585086, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=319, Percent_Identity=33.2288401253918, Blast_Score=153, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6320560, Length=317, Percent_Identity=32.4921135646688, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321898, Length=314, Percent_Identity=31.2101910828025, Blast_Score=141, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56013; Mature: 56013

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDSNLKAQVKTYLENVTQPIELVASLDESAKSEEMLDLLEDIAGLSDKITLIGRDDEGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
KPSFSINRAGTDIGVRFAGLPMGHEFNSLILALLHVGGHPSKTAPEVVEQIESLDGDYNF
CCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCE
ETYFSLSCQNCPDVVQALNLMSALNPRIKHTAIDGALFQDEVESRQVMAVPAIYLNGEPF
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCEEEEEEEEEECCCCC
AQGRMTLEQILAKLDTGAAERTAAKLKDKEAFDVLVIGGGPAGAAAAIYAARKGIRTGVA
CHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCH
AERFGGQVLDTMGIENFISVAHTEGPKLAAALESHVKDYAVDIMNLQRAEKLIPAKTPGG
HHHHCCHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVEVQLASGAALKARTVILSTGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCEEEEEEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLIEFDARLRADQVLQTKLASLNNVTIITSALTSEVHGDG
ECCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCC
AKVVGLSYKDRTTEEFHRIDLDGIFVQIGLVPNTEWLKGTVALSPRGEIEIDARGQTSLP
CEEEEEECCCCCHHHHHEECCCEEEEEEEECCCCHHHEEEEEECCCCCEEECCCCCCCCC
GVFAAGDATTVPYKQIIIAMGEGSKAALSAFDYLIRLPQAEETAAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MLDSNLKAQVKTYLENVTQPIELVASLDESAKSEEMLDLLEDIAGLSDKITLIGRDDEGR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCC
KPSFSINRAGTDIGVRFAGLPMGHEFNSLILALLHVGGHPSKTAPEVVEQIESLDGDYNF
CCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCE
ETYFSLSCQNCPDVVQALNLMSALNPRIKHTAIDGALFQDEVESRQVMAVPAIYLNGEPF
EEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCEEEEEEEEEECCCCC
AQGRMTLEQILAKLDTGAAERTAAKLKDKEAFDVLVIGGGPAGAAAAIYAARKGIRTGVA
CHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCH
AERFGGQVLDTMGIENFISVAHTEGPKLAAALESHVKDYAVDIMNLQRAEKLIPAKTPGG
HHHHCCHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
LVEVQLASGAALKARTVILSTGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCEEEEEEEEECCCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLIEFDARLRADQVLQTKLASLNNVTIITSALTSEVHGDG
ECCCCCHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHCCCC
AKVVGLSYKDRTTEEFHRIDLDGIFVQIGLVPNTEWLKGTVALSPRGEIEIDARGQTSLP
CEEEEEECCCCCHHHHHEECCCEEEEEEEECCCCHHHEEEEEECCCCCEEECCCCCCCCC
GVFAAGDATTVPYKQIIIAMGEGSKAALSAFDYLIRLPQAEETAAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]