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The map label for this gene is lutB [H]

Identifier: 83592748

GI number: 83592748

Start: 1669441

End: 1670895

Strand: Reverse

Name: lutB [H]

Synonym: Rru_A1412

Alternate gene names: 83592748

Gene position: 1670895-1669441 (Counterclockwise)

Preceding gene: 83592749

Following gene: 83592747

Centisome position: 38.39

GC content: 67.97

Gene sequence:

>1455_bases
ATGAACGCCCCCCTTCCCCAGGACACCGCCGCCACCGCCCCCTTGGCCTTCACCGGCAAGGCCAGGGTGGCTTTGAACGA
TCCGCGCCTGCGCGCCAATTTCCGCCGGGCGATGGACGGCCTGATGAGCAAGCGCGCCGCCCAGTTCGCCGATGCCGACG
AATGGCGGACCTTGCGCGCCCGTGGCGCCGAAGCCCGCGCCCGCGCCCTGGCCAAACTGCCCGACTTGCTCGAACGCCTG
GAGCGACGCTGCGCCGAAAACGCCATCACCGTCCATTGGGCCGAAACCACCGGGCAAGCCAACGCCATCGTTCTCGATCT
CTTGACCGCCGCCGGGGCGAAGACGGTGATCAAAGGCAAATCGATGGTCAGCGAGGAGATGCACCTCAATGCCCATCTCG
CCGCCCACGGCATCACCGCCATCGAATCCGATCTGGGCGAATACATCATCCAACTGGCCGGCGAGGCGCCCTCGCATATC
GTCATGCCCTGCATCCACAAGAACAAGGCCGAGATCGCCGCGCTGTTCGCCGAGCATATCCCCGGCCAGCCCTATACCGA
AAACGTCGATGATCTGACGGGCGCCGCCCGCAACGCCCTGCGCGGTGCCTTCGCCGCCGCCGATGCCGGGATCTCGGGGG
TGAATTTCGCCGTCGCCGAAACCGGCACCCTGGTGCTGATCGAGAACGAGGGCAACGGCCGGCTGTCCACCACCCTGCCG
CCCCTGCATATCGCCGTGACCGGCATCGAGAAGGTGCTGGAATTCCTCGATGACGTGCCGCCGCTGCTGTCGTTGCTGCC
GCGCTCGGCCACCGGCCAGCCGATCACCACCTATGTCAATATGATCAGCGGCCCGCGCCGCCCCGGCGAAAAGGACGGGC
CGCAAGCGGTCCATCTGGTGTTGCTCGACAACGGCCGCTCGCGGGTTTACGACGACCCGCAATTGCGCGACACCCTGCGT
TGCATCCGCTGCGCCGCCTGTCTCAACCATTGCCCGGTCTATACCCGGGTCGGCGGCCATAGCTATTCCTTCACCTATCC
CGGGCCGATCGGCAAAATCCTCAGCCCCCAGCTTGAGGGCCTCGATTGCGCCGGCGATCAGCCCCACGCCTCAAGCCTGT
GCGGCGCCTGCGCCGAGGTTTGCCCGGTCGAGATCCCCATCCCCGATTTGCTCGTGCGCCTGCGCGGCGAGGCGATCAGC
CCGACCTCGCGGGCGGTCAAGGGCGGGGGCAGCGCGCGAAGCCTGGGCGATCGCCTGGGGCGCCGGCTGTCCACCGCCAT
TCTTGGTCAGCCCCGGCTGTATGGCCTGATCAGCCGCTTGGCCGGGCGGATCGGCGATCGCCTGCCGTCCTGGCTGCCCG
GACTGAACCGCTGGACGCGGGTGCGTAGCAAGCCCGCCTTCGCCCGCCGCAGCCTGCACGCCCTGGCCCGCGAAAGGGGC
TATGCCGATGAATGA

Upstream 100 bases:

>100_bases
GCGATTGCGGCTGCCTGATGAACATCAAAGGGGCGCTGGAAAAGGCCGGCAGACCGCAAAAAGCCCGCCATATCGCCGAA
TTCCTGGTCGAGCGCGGCCG

Downstream 100 bases:

>100_bases
CACCGCCCGCGCCGCCATCCTGGCCCGCCTGCGCGCCGCCCCGCGATCCCCCGCCCCGGCCTATCCGCGCCCCGGAACGC
CGGCCTGGGCGCCCCCCGTC

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MNAPLPQDTAATAPLAFTGKARVALNDPRLRANFRRAMDGLMSKRAAQFADADEWRTLRARGAEARARALAKLPDLLERL
ERRCAENAITVHWAETTGQANAIVLDLLTAAGAKTVIKGKSMVSEEMHLNAHLAAHGITAIESDLGEYIIQLAGEAPSHI
VMPCIHKNKAEIAALFAEHIPGQPYTENVDDLTGAARNALRGAFAAADAGISGVNFAVAETGTLVLIENEGNGRLSTTLP
PLHIAVTGIEKVLEFLDDVPPLLSLLPRSATGQPITTYVNMISGPRRPGEKDGPQAVHLVLLDNGRSRVYDDPQLRDTLR
CIRCAACLNHCPVYTRVGGHSYSFTYPGPIGKILSPQLEGLDCAGDQPHASSLCGACAEVCPVEIPIPDLLVRLRGEAIS
PTSRAVKGGGSARSLGDRLGRRLSTAILGQPRLYGLISRLAGRIGDRLPSWLPGLNRWTRVRSKPAFARRSLHALARERG
YADE

Sequences:

>Translated_484_residues
MNAPLPQDTAATAPLAFTGKARVALNDPRLRANFRRAMDGLMSKRAAQFADADEWRTLRARGAEARARALAKLPDLLERL
ERRCAENAITVHWAETTGQANAIVLDLLTAAGAKTVIKGKSMVSEEMHLNAHLAAHGITAIESDLGEYIIQLAGEAPSHI
VMPCIHKNKAEIAALFAEHIPGQPYTENVDDLTGAARNALRGAFAAADAGISGVNFAVAETGTLVLIENEGNGRLSTTLP
PLHIAVTGIEKVLEFLDDVPPLLSLLPRSATGQPITTYVNMISGPRRPGEKDGPQAVHLVLLDNGRSRVYDDPQLRDTLR
CIRCAACLNHCPVYTRVGGHSYSFTYPGPIGKILSPQLEGLDCAGDQPHASSLCGACAEVCPVEIPIPDLLVRLRGEAIS
PTSRAVKGGGSARSLGDRLGRRLSTAILGQPRLYGLISRLAGRIGDRLPSWLPGLNRWTRVRSKPAFARRSLHALARERG
YADE
>Mature_484_residues
MNAPLPQDTAATAPLAFTGKARVALNDPRLRANFRRAMDGLMSKRAAQFADADEWRTLRARGAEARARALAKLPDLLERL
ERRCAENAITVHWAETTGQANAIVLDLLTAAGAKTVIKGKSMVSEEMHLNAHLAAHGITAIESDLGEYIIQLAGEAPSHI
VMPCIHKNKAEIAALFAEHIPGQPYTENVDDLTGAARNALRGAFAAADAGISGVNFAVAETGTLVLIENEGNGRLSTTLP
PLHIAVTGIEKVLEFLDDVPPLLSLLPRSATGQPITTYVNMISGPRRPGEKDGPQAVHLVLLDNGRSRVYDDPQLRDTLR
CIRCAACLNHCPVYTRVGGHSYSFTYPGPIGKILSPQLEGLDCAGDQPHASSLCGACAEVCPVEIPIPDLLVRLRGEAIS
PTSRAVKGGGSARSLGDRLGRRLSTAILGQPRLYGLISRLAGRIGDRLPSWLPGLNRWTRVRSKPAFARRSLHALARERG
YADE

Specific function: Is essential for L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate. May also allow cells to utilize an alternative carbon source during biofi

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=384, Percent_Identity=41.6666666666667, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 51954; Mature: 51954

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPLPQDTAATAPLAFTGKARVALNDPRLRANFRRAMDGLMSKRAAQFADADEWRTLRA
CCCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RGAEARARALAKLPDLLERLERRCAENAITVHWAETTGQANAIVLDLLTAAGAKTVIKGK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHH
SMVSEEMHLNAHLAAHGITAIESDLGEYIIQLAGEAPSHIVMPCIHKNKAEIAALFAEHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHC
PGQPYTENVDDLTGAARNALRGAFAAADAGISGVNFAVAETGTLVLIENEGNGRLSTTLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCEEECCCC
PLHIAVTGIEKVLEFLDDVPPLLSLLPRSATGQPITTYVNMISGPRRPGEKDGPQAVHLV
CHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEE
LLDNGRSRVYDDPQLRDTLRCIRCAACLNHCPVYTRVGGHSYSFTYPGPIGKILSPQLEG
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHCCCCCCC
LDCAGDQPHASSLCGACAEVCPVEIPIPDLLVRLRGEAISPTSRAVKGGGSARSLGDRLG
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHH
RRLSTAILGQPRLYGLISRLAGRIGDRLPSWLPGLNRWTRVRSKPAFARRSLHALARERG
HHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
YADE
CCCC
>Mature Secondary Structure
MNAPLPQDTAATAPLAFTGKARVALNDPRLRANFRRAMDGLMSKRAAQFADADEWRTLRA
CCCCCCCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
RGAEARARALAKLPDLLERLERRCAENAITVHWAETTGQANAIVLDLLTAAGAKTVIKGK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHH
SMVSEEMHLNAHLAAHGITAIESDLGEYIIQLAGEAPSHIVMPCIHKNKAEIAALFAEHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHC
PGQPYTENVDDLTGAARNALRGAFAAADAGISGVNFAVAETGTLVLIENEGNGRLSTTLP
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEECCCCEEECCCC
PLHIAVTGIEKVLEFLDDVPPLLSLLPRSATGQPITTYVNMISGPRRPGEKDGPQAVHLV
CHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEE
LLDNGRSRVYDDPQLRDTLRCIRCAACLNHCPVYTRVGGHSYSFTYPGPIGKILSPQLEG
EEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCHHHCCCCCCC
LDCAGDQPHASSLCGACAEVCPVEIPIPDLLVRLRGEAISPTSRAVKGGGSARSLGDRLG
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHCCCCCHHHHHHHHH
RRLSTAILGQPRLYGLISRLAGRIGDRLPSWLPGLNRWTRVRSKPAFARRSLHALARERG
HHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHCC
YADE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]