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The map label for this gene is puuA [H]

Identifier: 83592589

GI number: 83592589

Start: 1473824

End: 1475188

Strand: Reverse

Name: puuA [H]

Synonym: Rru_A1253

Alternate gene names: 83592589

Gene position: 1475188-1473824 (Counterclockwise)

Preceding gene: 83592590

Following gene: 83592588

Centisome position: 33.89

GC content: 68.5

Gene sequence:

>1365_bases
ATGACGGTTCTTCCCCCCGCCGATGGCGGCGCCTTGTTGCGCGAAGAGGTGCGGCGTTTCCGCGAGGCCCATCCCGATAT
CGCCTATGTCGATCTGCTGTCGCTGGATCTGCCCGGGCATTTCTATGGCAAGCGCCTGCCGATCGCCATGCTCGACGATA
TCGCCGCCGGCGCGCCGCTCAAGCTGCCGCAAAACGCCATTATCCTCGGTGCCACCGGCGGCGTCTATCCGATCGGCCGC
TATGCCTTGGAAGACGGCGATCCCGATGCGCCGCGCCGTCTGGTCGCCGGCACCCTGGCCCCCGTGCCCTGGGAAAGCCG
GCCGACCGCCCAGATGCTGGTGACCTCCAGCGGCACCGATCACCCGGTGTGCTTCGAGCCGCGCGAGGTTCTGAACGGCG
TCGTCGAACGGCTGCGCGCCAAGGGTCTGTTCCCCACCGTCGCCTTCGAGCTGGAATTCTATCTGCTGTCCCAAGACCGC
CCCGACGGCCTGATCGCCCCGCCGCGCGACCCGCTGTCGGGCCGGGCCGATCTGCATCCGGCCATGCATATCGAGCGGCT
AAGCCGCTTTTCGGACGTGCTGCACGCCATCATCGACGCCGCCCAGGCCCAGGGCATCGAGACGACGGCGATGAGCGCGG
AAGTCGGCCTGGGGCAATACGAGATCAATTTCGCCCATTGCGCCGATCCGCTGCGCGCCGCCGACTGGTCGGCGCTGTTT
TGCCGAGCAACGCGCGGCGTGGCGATGCGCCATGGCTATCAGGCGACCTTCCTGTCCAAGCCCTATCTGCAGAGCGCGGG
CAGCGGCATGCATCTGCATGTCAGCCTGTGCGACGAGCGCGGCGCCAATCTGCTCGCCCCGGAGAACGCGCTCGGCCTGA
AGCGCGCCGTCGCCGGCTGTCTGGCCCTGATGCCGGCCAGCGTCGCCCTGTTCTCGGCCAATCACAACGCCTTCCGCCGC
TCGGCCAGCGGCGCGGTCAATACGGCGGCCGGTGGCTGCTGGGGCTGGGACGATCGCTCGGCGGGCATCCGCATCCCGCC
CTCGGGTCCGGCGGCTTTGCGCCTGGAACACCGGGTGGCCGGGGCCGACGCCAATCCCTATCTGGCGCTGGCCGCCATCC
TTGCCGGGATGGACTACGGCCTTGAGTGCGCCCTTGATCCCCGCCCCACCCGCGAGGAGGCCCCAAGCGCCAGCGTCGCC
CTGCCCGGCGATATGCTCGAGGCCCTGCGCACGATGGACGCCAGCGCCGAACTCCGCGCCCGCCTGGGCGATGAGTTCGT
CACCGTCTACACCACCATGAAGCGCCAGGATCATCTGGCTTTCCTGGGGATGATCAGTCCTCGGGAATACGACTGGTATT
TGTGA

Upstream 100 bases:

>100_bases
TGATCATCAGCCGTGACGAGGTCGATGAGCTGGTTCGTCTGGCCCGCCGCTGCTTCGATCTGACCCTGCGCGATGTCGGC
CCCCTGATCGAGACGGCCAA

Downstream 100 bases:

>100_bases
CATCAAGGGGCCCATTGTAGGGCCCCTTCTTTTTTGACGGCTCCAACCGTCCTGCCCGGCGTCGGCGTCCTTCCGGTCCC
CGGCGGCAACCATAAGACGA

Product: glutamine synthetase, catalytic region

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTVLPPADGGALLREEVRRFREAHPDIAYVDLLSLDLPGHFYGKRLPIAMLDDIAAGAPLKLPQNAIILGATGGVYPIGR
YALEDGDPDAPRRLVAGTLAPVPWESRPTAQMLVTSSGTDHPVCFEPREVLNGVVERLRAKGLFPTVAFELEFYLLSQDR
PDGLIAPPRDPLSGRADLHPAMHIERLSRFSDVLHAIIDAAQAQGIETTAMSAEVGLGQYEINFAHCADPLRAADWSALF
CRATRGVAMRHGYQATFLSKPYLQSAGSGMHLHVSLCDERGANLLAPENALGLKRAVAGCLALMPASVALFSANHNAFRR
SASGAVNTAAGGCWGWDDRSAGIRIPPSGPAALRLEHRVAGADANPYLALAAILAGMDYGLECALDPRPTREEAPSASVA
LPGDMLEALRTMDASAELRARLGDEFVTVYTTMKRQDHLAFLGMISPREYDWYL

Sequences:

>Translated_454_residues
MTVLPPADGGALLREEVRRFREAHPDIAYVDLLSLDLPGHFYGKRLPIAMLDDIAAGAPLKLPQNAIILGATGGVYPIGR
YALEDGDPDAPRRLVAGTLAPVPWESRPTAQMLVTSSGTDHPVCFEPREVLNGVVERLRAKGLFPTVAFELEFYLLSQDR
PDGLIAPPRDPLSGRADLHPAMHIERLSRFSDVLHAIIDAAQAQGIETTAMSAEVGLGQYEINFAHCADPLRAADWSALF
CRATRGVAMRHGYQATFLSKPYLQSAGSGMHLHVSLCDERGANLLAPENALGLKRAVAGCLALMPASVALFSANHNAFRR
SASGAVNTAAGGCWGWDDRSAGIRIPPSGPAALRLEHRVAGADANPYLALAAILAGMDYGLECALDPRPTREEAPSASVA
LPGDMLEALRTMDASAELRARLGDEFVTVYTTMKRQDHLAFLGMISPREYDWYL
>Mature_453_residues
TVLPPADGGALLREEVRRFREAHPDIAYVDLLSLDLPGHFYGKRLPIAMLDDIAAGAPLKLPQNAIILGATGGVYPIGRY
ALEDGDPDAPRRLVAGTLAPVPWESRPTAQMLVTSSGTDHPVCFEPREVLNGVVERLRAKGLFPTVAFELEFYLLSQDRP
DGLIAPPRDPLSGRADLHPAMHIERLSRFSDVLHAIIDAAQAQGIETTAMSAEVGLGQYEINFAHCADPLRAADWSALFC
RATRGVAMRHGYQATFLSKPYLQSAGSGMHLHVSLCDERGANLLAPENALGLKRAVAGCLALMPASVALFSANHNAFRRS
ASGAVNTAAGGCWGWDDRSAGIRIPPSGPAALRLEHRVAGADANPYLALAAILAGMDYGLECALDPRPTREEAPSASVAL
PGDMLEALRTMDASAELRARLGDEFVTVYTTMKRQDHLAFLGMISPREYDWYL

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=362, Percent_Identity=23.7569060773481, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI87081870, Length=456, Percent_Identity=37.0614035087719, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI1790301, Length=367, Percent_Identity=26.9754768392371, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 48889; Mature: 48758

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLPPADGGALLREEVRRFREAHPDIAYVDLLSLDLPGHFYGKRLPIAMLDDIAAGAPL
CCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
KLPQNAIILGATGGVYPIGRYALEDGDPDAPRRLVAGTLAPVPWESRPTAQMLVTSSGTD
CCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCC
HPVCFEPREVLNGVVERLRAKGLFPTVAFELEFYLLSQDRPDGLIAPPRDPLSGRADLHP
CCCEECHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCH
AMHIERLSRFSDVLHAIIDAAQAQGIETTAMSAEVGLGQYEINFAHCADPLRAADWSALF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEHHHHCCCHHHCCHHHHH
CRATRGVAMRHGYQATFLSKPYLQSAGSGMHLHVSLCDERGANLLAPENALGLKRAVAGC
HHHHCCHHHHCCCCEEECCCHHHHHCCCCEEEEEEEECCCCCCEECCCHHHHHHHHHHHH
LALMPASVALFSANHNAFRRSASGAVNTAAGGCWGWDDRSAGIRIPPSGPAALRLEHRVA
HHHHHHHEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEEEEC
GADANPYLALAAILAGMDYGLECALDPRPTREEAPSASVALPGDMLEALRTMDASAELRA
CCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCCCEECCHHHHHHHHHCCCCHHHHH
RLGDEFVTVYTTMKRQDHLAFLGMISPREYDWYL
HHCCCEEEEEEHHHCCCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TVLPPADGGALLREEVRRFREAHPDIAYVDLLSLDLPGHFYGKRLPIAMLDDIAAGAPL
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHCCCCC
KLPQNAIILGATGGVYPIGRYALEDGDPDAPRRLVAGTLAPVPWESRPTAQMLVTSSGTD
CCCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCCCEEEEEECCCCC
HPVCFEPREVLNGVVERLRAKGLFPTVAFELEFYLLSQDRPDGLIAPPRDPLSGRADLHP
CCCEECHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCH
AMHIERLSRFSDVLHAIIDAAQAQGIETTAMSAEVGLGQYEINFAHCADPLRAADWSALF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEHHHHCCCHHHCCHHHHH
CRATRGVAMRHGYQATFLSKPYLQSAGSGMHLHVSLCDERGANLLAPENALGLKRAVAGC
HHHHCCHHHHCCCCEEECCCHHHHHCCCCEEEEEEEECCCCCCEECCCHHHHHHHHHHHH
LALMPASVALFSANHNAFRRSASGAVNTAAGGCWGWDDRSAGIRIPPSGPAALRLEHRVA
HHHHHHHEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCEEEEEEEEC
GADANPYLALAAILAGMDYGLECALDPRPTREEAPSASVALPGDMLEALRTMDASAELRA
CCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHCCCCCEECCHHHHHHHHHCCCCHHHHH
RLGDEFVTVYTTMKRQDHLAFLGMISPREYDWYL
HHCCCEEEEEEHHHCCCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]