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The map label for this gene is puuA [H]

Identifier: 83592588

GI number: 83592588

Start: 1472361

End: 1473713

Strand: Reverse

Name: puuA [H]

Synonym: Rru_A1252

Alternate gene names: 83592588

Gene position: 1473713-1472361 (Counterclockwise)

Preceding gene: 83592589

Following gene: 83592585

Centisome position: 33.86

GC content: 61.57

Gene sequence:

>1353_bases
TTGATCAACGAGTTCGAAGCCTGGATCAACGAGCATGGCATCGCGGAAGTCGAATGCCTGCTCTCGGACATGAATGGCAT
CGTCCGCGGAAAGGTGCTGCCCGCCCGAAAATTCCTGCGCTCGCTGGAAGACAACTCGCTGCGCATTCCAAGCAGCGTGT
TTCTGGTGACAGTGACGGGCGAATACCCCTTCGCCGATGACAACAACACCGTCGTCATCGACCCCGACGCCGACCTCAGG
CCCGATTTCCGCACGTTATGCGTCGCGCCGGGCTACCGCACGCCCACCGCCTTCGTCGTTGTCGACGCCTATAAAAGCAA
CGGCATCCCGCTTGATCTCTCGCCCAGACAGGTGCTGAAGCGGGTTTTGGCGCTCTATTCTGAACAGGGCTGGAAGCCGG
TCGTCGCCCCCGAGATCGAGTTTTATCTGACCCAGGTCAACGCCGATCCCGATCTGCCGCTATCGCCGCCCGCCGGCCGT
TCGGGACGGCCCGAAACCTCGCCCCAGCCCTATGGTCTGGAAGCGATTACCGAATTCGAAGACCTGATCGAGAATATCTA
CGAACACGCCGAAATCGCCGGCCTGCAGATCGATACCCTGATCCATGAGGCCGGCGCCGCCCAGTTGGAGATCAATTTCC
TTCACGGCGACCCGATCACGCTCTCGGATCAGGTGGTGGTGTTCAAGCGTCTGGTGCGTCAGGTGGCGCTCAAGCACGGG
GTCTATGCCACCTTCATGGCCAAGCCGATGGCCGATCAGCCGGGCAGCGCCATGCATATTCACCAGTCCCTGGTGGAAAT
CGGCACCGGCAAGAACCTGTTCGTGCGCGACGATGGCAGCGACAGCCCGATGTTTCGCCATTATATCGGCGGCCTGCAGG
CCCTGCTCGCCCAGGTGACGCCGCTGTTCGCCCCCAACATCAATTCCTTCCGCCGCATGCGCCCCCATCACAGCGCGCCG
ATCAATCTGGAATGCGGCTATGACAACCGCTCTTGCGGGCTGCGGGTGCCCTTGTCCGATCCGGCCAACCGCCGCCTGGA
AAACCGCCTGCCCGGCGCCGACGCCAATCCCTATCTGGCGATGGCCGCCTCGCTGGCCTGCGGCTATATCGGCATCATGG
AGCGGATCGAGCCCTCGATCATGGTCGAAGGCGGCAATGCCTATCGCCGCTCGCGCACCCTGCCGCGCACCCTTGATGAA
GCCCTTGACCGCTTGGAGAAATGCACGGTCATCAAGAACGTGCTGGGCGAGCACTTCTTCGAAGCCTTCACCATGATCAA
GCGCGTCGAACTCGACGCCTTCCAAGACGTCATCAGTTCGTGGGAGCGCAATCACCTGCTGCTCAAGGTGTAA

Upstream 100 bases:

>100_bases
CCCATTGTAGGGCCCCTTCTTTTTTGACGGCTCCAACCGTCCTGCCCGGCGTCGGCGTCCTTCCGGTCCCCGGCGGCAAC
CATAAGACGATAGGTGCACT

Downstream 100 bases:

>100_bases
GGTAAGCGGCGTTAAACCGGATACTCTTCCTGGCGCGCCACCCGGCGGCGACGGTCGGGGCGGCCGGGGGGATGTTTGTA
GTGATCGCCATCCCGGCATT

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MINEFEAWINEHGIAEVECLLSDMNGIVRGKVLPARKFLRSLEDNSLRIPSSVFLVTVTGEYPFADDNNTVVIDPDADLR
PDFRTLCVAPGYRTPTAFVVVDAYKSNGIPLDLSPRQVLKRVLALYSEQGWKPVVAPEIEFYLTQVNADPDLPLSPPAGR
SGRPETSPQPYGLEAITEFEDLIENIYEHAEIAGLQIDTLIHEAGAAQLEINFLHGDPITLSDQVVVFKRLVRQVALKHG
VYATFMAKPMADQPGSAMHIHQSLVEIGTGKNLFVRDDGSDSPMFRHYIGGLQALLAQVTPLFAPNINSFRRMRPHHSAP
INLECGYDNRSCGLRVPLSDPANRRLENRLPGADANPYLAMAASLACGYIGIMERIEPSIMVEGGNAYRRSRTLPRTLDE
ALDRLEKCTVIKNVLGEHFFEAFTMIKRVELDAFQDVISSWERNHLLLKV

Sequences:

>Translated_450_residues
MINEFEAWINEHGIAEVECLLSDMNGIVRGKVLPARKFLRSLEDNSLRIPSSVFLVTVTGEYPFADDNNTVVIDPDADLR
PDFRTLCVAPGYRTPTAFVVVDAYKSNGIPLDLSPRQVLKRVLALYSEQGWKPVVAPEIEFYLTQVNADPDLPLSPPAGR
SGRPETSPQPYGLEAITEFEDLIENIYEHAEIAGLQIDTLIHEAGAAQLEINFLHGDPITLSDQVVVFKRLVRQVALKHG
VYATFMAKPMADQPGSAMHIHQSLVEIGTGKNLFVRDDGSDSPMFRHYIGGLQALLAQVTPLFAPNINSFRRMRPHHSAP
INLECGYDNRSCGLRVPLSDPANRRLENRLPGADANPYLAMAASLACGYIGIMERIEPSIMVEGGNAYRRSRTLPRTLDE
ALDRLEKCTVIKNVLGEHFFEAFTMIKRVELDAFQDVISSWERNHLLLKV
>Mature_450_residues
MINEFEAWINEHGIAEVECLLSDMNGIVRGKVLPARKFLRSLEDNSLRIPSSVFLVTVTGEYPFADDNNTVVIDPDADLR
PDFRTLCVAPGYRTPTAFVVVDAYKSNGIPLDLSPRQVLKRVLALYSEQGWKPVVAPEIEFYLTQVNADPDLPLSPPAGR
SGRPETSPQPYGLEAITEFEDLIENIYEHAEIAGLQIDTLIHEAGAAQLEINFLHGDPITLSDQVVVFKRLVRQVALKHG
VYATFMAKPMADQPGSAMHIHQSLVEIGTGKNLFVRDDGSDSPMFRHYIGGLQALLAQVTPLFAPNINSFRRMRPHHSAP
INLECGYDNRSCGLRVPLSDPANRRLENRLPGADANPYLAMAASLACGYIGIMERIEPSIMVEGGNAYRRSRTLPRTLDE
ALDRLEKCTVIKNVLGEHFFEAFTMIKRVELDAFQDVISSWERNHLLLKV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=439, Percent_Identity=25.2847380410023, Blast_Score=114, Evalue=3e-25,
Organism=Escherichia coli, GI87081870, Length=441, Percent_Identity=33.7868480725624, Blast_Score=224, Evalue=9e-60,
Organism=Escherichia coli, GI1790301, Length=459, Percent_Identity=27.2331154684096, Blast_Score=125, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50157; Mature: 50157

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINEFEAWINEHGIAEVECLLSDMNGIVRGKVLPARKFLRSLEDNSLRIPSSVFLVTVTG
CCCHHHHHHHHCCCHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCEECCCEEEEEEEEC
EYPFADDNNTVVIDPDADLRPDFRTLCVAPGYRTPTAFVVVDAYKSNGIPLDLSPRQVLK
CCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHH
RVLALYSEQGWKPVVAPEIEFYLTQVNADPDLPLSPPAGRSGRPETSPQPYGLEAITEFE
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DLIENIYEHAEIAGLQIDTLIHEAGAAQLEINFLHGDPITLSDQVVVFKRLVRQVALKHG
HHHHHHHHHHHHCCEEHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
VYATFMAKPMADQPGSAMHIHQSLVEIGTGKNLFVRDDGSDSPMFRHYIGGLQALLAQVT
CCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
PLFAPNINSFRRMRPHHSAPINLECGYDNRSCGLRVPLSDPANRRLENRLPGADANPYLA
HHCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCCCHHHH
MAASLACGYIGIMERIEPSIMVEGGNAYRRSRTLPRTLDEALDRLEKCTVIKNVLGEHFF
HHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFTMIKRVELDAFQDVISSWERNHLLLKV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MINEFEAWINEHGIAEVECLLSDMNGIVRGKVLPARKFLRSLEDNSLRIPSSVFLVTVTG
CCCHHHHHHHHCCCHHHHHHHHHHCCHHCCCCCHHHHHHHHHCCCCEECCCEEEEEEEEC
EYPFADDNNTVVIDPDADLRPDFRTLCVAPGYRTPTAFVVVDAYKSNGIPLDLSPRQVLK
CCCCCCCCCEEEECCCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCCCCCCHHHHHH
RVLALYSEQGWKPVVAPEIEFYLTQVNADPDLPLSPPAGRSGRPETSPQPYGLEAITEFE
HHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
DLIENIYEHAEIAGLQIDTLIHEAGAAQLEINFLHGDPITLSDQVVVFKRLVRQVALKHG
HHHHHHHHHHHHCCEEHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
VYATFMAKPMADQPGSAMHIHQSLVEIGTGKNLFVRDDGSDSPMFRHYIGGLQALLAQVT
CCEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
PLFAPNINSFRRMRPHHSAPINLECGYDNRSCGLRVPLSDPANRRLENRLPGADANPYLA
HHCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCCCHHHH
MAASLACGYIGIMERIEPSIMVEGGNAYRRSRTLPRTLDEALDRLEKCTVIKNVLGEHFF
HHHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EAFTMIKRVELDAFQDVISSWERNHLLLKV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]