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The map label for this gene is proB

Identifier: 83592575

GI number: 83592575

Start: 1461951

End: 1463078

Strand: Reverse

Name: proB

Synonym: Rru_A1239

Alternate gene names: 83592575

Gene position: 1463078-1461951 (Counterclockwise)

Preceding gene: 83592576

Following gene: 83592574

Centisome position: 33.61

GC content: 69.77

Gene sequence:

>1128_bases
GTGATCAAGATCGGCTCGGCCCTTCTCGTCGATCAGGGCAGCGGGCTGGTGCGCACCGGCTGGCTCAAGGCCCTGGCCGA
AGACATCGCCCAGCTGCGCGCCGAAGGGCGCGAGGTGCTGATCGTCTCCTCGGGGGCGATCGCCGTCGGCCGGCGCTCGC
TGCATCTGGAACCGGGCCGCATGCGGCTTGATGAAAAGCAGGCCGCCGCCGCCGCCGGCCAGATCGAACTGGCCCATGCC
TATCGCACGGTGATGGCCGATCACGCCATCAACGTCGCCCAATTGCTGCTGACCCTTGATGACTCCGAGAACCGCCGGCG
TTATCTCAACGCCCGCAACTGCCTGGAAACCCTGCTCCGGCTAGGCGTCATCCCGGTCATCAACGAAAACGACACCGTCA
CCACCCAGGAAATCCGCTTTGGCGACAACGACCGGCTGGCCGCCCGCGTCGCCCAGATGGCCTGCGCCGACGCGTTGATC
CTGCTTTCGGATATCGATGGCCTTTATACCGCCGATCCCCACCGCGATCCCGAGGCCCGCTTCATCCCCACCGTCACCGC
CCTGACCCCGGAGATCGCCGCCATGGCCGGGGCGGCGCTGACCCCGCATGGCACCGGCGGCATGGTCACCAAGCTTCTGG
CCGCGCGCATCTGCATGGATGCCGGCTGCGCCATGGCGATCGCCCCGGGCAAGGACGACCACGCCTTGCGCCTGCTGCGC
CAGGGCGGCCGCCAGACCTGGTTCCTGCCGGCCGAAGAGCCGCGCACGGCGCGCAAGGCATGGATTTCCGGGGCGCTGAA
GGCCGAGGGGACCCTGTGGGTCGATGACGGCGCCGCCCGGGCGCTGGCGGCGGGCAGCAGCCTGTTGCCGCCGGGCGTGC
GCACGGTCGAGGGCGATTTCCAGCGCGGCGCCGCCGTGCGGGTGGTCGGGCTCGACGGCAGCCCGCTGGCCAAGGGGCTG
ATCGCCTATCCCTCCGACGAGGCGCGTTTGCTCGTCGGCCATCGCAGCGGCGACATCGAGGCGCTGCTGGGATACCGTGG
GCGCGAGGAACTGATCCACCGCGATGATCTGGTGCTCGACAGCCGCTTCCGGCACCGCGACGAGGACGGGGAAGGACACC
GATCGTGA

Upstream 100 bases:

>100_bases
ACGACGATGGGGACGAGAGCGGAGACAAGGGACAGTGGACACGCTGAACGCAACCGCCGCCGTCGATGCCGAGGCCATTC
TCGGCAACGCCCGGCGGCTG

Downstream 100 bases:

>100_bases
ACGACGCCGCACGCCTGACCACCGATGATATTCCGGGGCTGATGATCGACATCGGCCGCCGGGCCCGCGCCGCCGGCCGC
ATCCTGGCCCTGGCGCCCGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIKIGSALLVDQGSGLVRTGWLKALAEDIAQLRAEGREVLIVSSGAIAVGRRSLHLEPGRMRLDEKQAAAAAGQIELAHA
YRTVMADHAINVAQLLLTLDDSENRRRYLNARNCLETLLRLGVIPVINENDTVTTQEIRFGDNDRLAARVAQMACADALI
LLSDIDGLYTADPHRDPEARFIPTVTALTPEIAAMAGAALTPHGTGGMVTKLLAARICMDAGCAMAIAPGKDDHALRLLR
QGGRQTWFLPAEEPRTARKAWISGALKAEGTLWVDDGAARALAAGSSLLPPGVRTVEGDFQRGAAVRVVGLDGSPLAKGL
IAYPSDEARLLVGHRSGDIEALLGYRGREELIHRDDLVLDSRFRHRDEDGEGHRS

Sequences:

>Translated_375_residues
MIKIGSALLVDQGSGLVRTGWLKALAEDIAQLRAEGREVLIVSSGAIAVGRRSLHLEPGRMRLDEKQAAAAAGQIELAHA
YRTVMADHAINVAQLLLTLDDSENRRRYLNARNCLETLLRLGVIPVINENDTVTTQEIRFGDNDRLAARVAQMACADALI
LLSDIDGLYTADPHRDPEARFIPTVTALTPEIAAMAGAALTPHGTGGMVTKLLAARICMDAGCAMAIAPGKDDHALRLLR
QGGRQTWFLPAEEPRTARKAWISGALKAEGTLWVDDGAARALAAGSSLLPPGVRTVEGDFQRGAAVRVVGLDGSPLAKGL
IAYPSDEARLLVGHRSGDIEALLGYRGREELIHRDDLVLDSRFRHRDEDGEGHRS
>Mature_375_residues
MIKIGSALLVDQGSGLVRTGWLKALAEDIAQLRAEGREVLIVSSGAIAVGRRSLHLEPGRMRLDEKQAAAAAGQIELAHA
YRTVMADHAINVAQLLLTLDDSENRRRYLNARNCLETLLRLGVIPVINENDTVTTQEIRFGDNDRLAARVAQMACADALI
LLSDIDGLYTADPHRDPEARFIPTVTALTPEIAAMAGAALTPHGTGGMVTKLLAARICMDAGCAMAIAPGKDDHALRLLR
QGGRQTWFLPAEEPRTARKAWISGALKAEGTLWVDDGAARALAAGSSLLPPGVRTVEGDFQRGAAVRVVGLDGSPLAKGL
IAYPSDEARLLVGHRSGDIEALLGYRGREELIHRDDLVLDSRFRHRDEDGEGHRS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=286, Percent_Identity=28.6713286713287, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI21361368, Length=288, Percent_Identity=28.4722222222222, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=41.6666666666667, Blast_Score=244, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17569969, Length=246, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17569967, Length=246, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=33.1730769230769, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=417, Percent_Identity=31.4148681055156, Blast_Score=170, Evalue=4e-43,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=31.7241379310345, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_RHORT (Q2RV05)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426327.1
- ProteinModelPortal:   Q2RV05
- SMR:   Q2RV05
- STRING:   Q2RV05
- GeneID:   3833737
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1239
- NMPDR:   fig|1085.1.peg.3127
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q2RV05
- ProtClustDB:   PRK05429
- BioCyc:   RRUB269796:RRU_A1239-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40322; Mature: 40322

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIGSALLVDQGSGLVRTGWLKALAEDIAQLRAEGREVLIVSSGAIAVGRRSLHLEPGR
CEEECCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCEEEECCCC
MRLDEKQAAAAAGQIELAHAYRTVMADHAINVAQLLLTLDDSENRRRYLNARNCLETLLR
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHH
LGVIPVINENDTVTTQEIRFGDNDRLAARVAQMACADALILLSDIDGLYTADPHRDPEAR
CCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCC
FIPTVTALTPEIAAMAGAALTPHGTGGMVTKLLAARICMDAGCAMAIAPGKDDHALRLLR
EECCHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHH
QGGRQTWFLPAEEPRTARKAWISGALKAEGTLWVDDGAARALAAGSSLLPPGVRTVEGDF
HCCCEEEEECCCCCHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCCCCCCCCEEECCCC
QRGAAVRVVGLDGSPLAKGLIAYPSDEARLLVGHRSGDIEALLGYRGREELIHRDDLVLD
CCCCEEEEEECCCCHHHCCEEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHCCEEEC
SRFRHRDEDGEGHRS
CHHHCCCCCCCCCCC
>Mature Secondary Structure
MIKIGSALLVDQGSGLVRTGWLKALAEDIAQLRAEGREVLIVSSGAIAVGRRSLHLEPGR
CEEECCEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCEEEECCCC
MRLDEKQAAAAAGQIELAHAYRTVMADHAINVAQLLLTLDDSENRRRYLNARNCLETLLR
CCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHH
LGVIPVINENDTVTTQEIRFGDNDRLAARVAQMACADALILLSDIDGLYTADPHRDPEAR
CCCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCC
FIPTVTALTPEIAAMAGAALTPHGTGGMVTKLLAARICMDAGCAMAIAPGKDDHALRLLR
EECCHHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHH
QGGRQTWFLPAEEPRTARKAWISGALKAEGTLWVDDGAARALAAGSSLLPPGVRTVEGDF
HCCCEEEEECCCCCHHHHHHHHCCCEECCCEEEEECCCHHHHHCCCCCCCCCCEEECCCC
QRGAAVRVVGLDGSPLAKGLIAYPSDEARLLVGHRSGDIEALLGYRGREELIHRDDLVLD
CCCCEEEEEECCCCHHHCCEEECCCCCCEEEEEECCCCHHHHHCCCCHHHHHHHCCEEEC
SRFRHRDEDGEGHRS
CHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA