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The map label for this gene is lpd3 [H]

Identifier: 83592551

GI number: 83592551

Start: 1435699

End: 1437099

Strand: Direct

Name: lpd3 [H]

Synonym: Rru_A1215

Alternate gene names: 83592551

Gene position: 1435699-1437099 (Clockwise)

Preceding gene: 83592550

Following gene: 83592555

Centisome position: 32.98

GC content: 65.1

Gene sequence:

>1401_bases
ATGTCCGATTTTGATGTCGTGGTGATCGGTGGCGGCCCGGGCGGCTACGTCTGTGCCATCCGCGCCGCTCAGCTTGGCCT
GCGGGTCGCCTGTGTCGAAAAGCGCCCGACGCTTGGCGGCACCTGCCTGAATGTCGGCTGCATTCCGTCCAAGGCGCTGT
TGCAGTCGTCGCACCTGTTCGACGAGGCCAACCACGGCTTCGCCGCCCATGGCATCACCGTCGGCACCCTGGGCCTCGAC
ATGAAGACGATGATGGCGCGCAAGGACGAGGTGGTCGAGGCCAACACCAAGGGCATCGTTTTCCTGTTCAAGAAGAACAA
GGTGACCCATGTCGTCGGCTCCGCCCGCATCGCCGGCGCCGGTCAGGTGGTGGTGAGCGGCGAGGGCGGCGAGCAGACGC
TGACGACGCGCGCCATCGTCATCGCCACCGGTTCGGAAGTCACGCCTTTGCCCGGAGTGACCATCGACGAGGAGCGGATC
GTCTCCTCGACCGGGGCGCTGGCCCTGGCCAAGGTGCCCAAATCCCTGGTGGTGATCGGCGCCGGGGTGATCGGCCTGGA
ATTGGGCTCGGTCTGGCGCCGCCTGGGCGCCGAGGTGACGGTTGTCGAATATCTCGACCACATCCTGCCGCCGATGGATG
GCGAGGTGCGCAAGCAAAGCCAGCGCATCCTGGAAAAGCAGGGACTGCGCTTCAAGCTGGGCCGCAAGGTGACGGCCGCC
GAACGCAAGGGCGAGGGGGTTAGTCTGACCGTCGAGCCGGCCAAGGGCGGCGAGGCCGAAACCCTGGAAGCCGAAACCGT
GCTGGTCGCCGTTGGCCGCCGGCCCTTTACCGACGGGCTGGGGCTTGAAGAGGTCGGCGTGGAAAAGACCGATCGCGGCT
TCGTCAAGGTCGATGGCGATTTCCAGACCGCTATCGAAGGCATCTTCGCCATCGGCGATGTGATCGGCGGCATGATGCTG
GCCCATAAGGCCGAGGACGAGGGCATGGCCCTGGCCGAGATGCTGGCCGGGGAAAGCGCCCATGTGAACTACGGCGCCAT
TCCGTCGGTTGTCTACACCTGGCCGGAAATCGCCGGGGTCGGCAAGACCGAGGAGATGCTGAAGAAGGACGGCGTCGCCT
ATAAGGTCGGCAAATTCCCCTTCACCGCCAATGCCCGGGCCAAGGCCAATGGCGACACCGACGGCTTCGTCAAGATCCTG
TCGAACGCCGAAACCGGCCGGGTGCTTGGCTGCCATATCATCGGCGCCCAGGCCGGCGATCTGATCATGGAAGTGGTTTT
GGGGATGGAATTCGGCGCCTCGTCCGAAGACATCGCCCGCACCTGCCACGCCCACCCGCAATTGGGCGAAGCGGTCAAGG
AAGCCGCCCTCGCCGTCAACAAGCGCACCATCCATATGTGA

Upstream 100 bases:

>100_bases
GGGCTTTGGTCGTTCCCCTCCCCATTCCCGAAAATCCACAAGTGCCTGTCCGCCGGGAAGGCCATCCCCCGGCGCCTGCA
AGGAAGAAGAGGCCAGCCTC

Downstream 100 bases:

>100_bases
GGGGTGGGCCAGCAGGCATAAAAAAACGGGCCGCGCTGGGGGGATCCCTGGCGCGGCCCGCTGTGTTTCTTGGCGGGTCG
AGAAAAACGGGCGGTTAGGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDFDVVVIGGGPGGYVCAIRAAQLGLRVACVEKRPTLGGTCLNVGCIPSKALLQSSHLFDEANHGFAAHGITVGTLGLD
MKTMMARKDEVVEANTKGIVFLFKKNKVTHVVGSARIAGAGQVVVSGEGGEQTLTTRAIVIATGSEVTPLPGVTIDEERI
VSSTGALALAKVPKSLVVIGAGVIGLELGSVWRRLGAEVTVVEYLDHILPPMDGEVRKQSQRILEKQGLRFKLGRKVTAA
ERKGEGVSLTVEPAKGGEAETLEAETVLVAVGRRPFTDGLGLEEVGVEKTDRGFVKVDGDFQTAIEGIFAIGDVIGGMML
AHKAEDEGMALAEMLAGESAHVNYGAIPSVVYTWPEIAGVGKTEEMLKKDGVAYKVGKFPFTANARAKANGDTDGFVKIL
SNAETGRVLGCHIIGAQAGDLIMEVVLGMEFGASSEDIARTCHAHPQLGEAVKEAALAVNKRTIHM

Sequences:

>Translated_466_residues
MSDFDVVVIGGGPGGYVCAIRAAQLGLRVACVEKRPTLGGTCLNVGCIPSKALLQSSHLFDEANHGFAAHGITVGTLGLD
MKTMMARKDEVVEANTKGIVFLFKKNKVTHVVGSARIAGAGQVVVSGEGGEQTLTTRAIVIATGSEVTPLPGVTIDEERI
VSSTGALALAKVPKSLVVIGAGVIGLELGSVWRRLGAEVTVVEYLDHILPPMDGEVRKQSQRILEKQGLRFKLGRKVTAA
ERKGEGVSLTVEPAKGGEAETLEAETVLVAVGRRPFTDGLGLEEVGVEKTDRGFVKVDGDFQTAIEGIFAIGDVIGGMML
AHKAEDEGMALAEMLAGESAHVNYGAIPSVVYTWPEIAGVGKTEEMLKKDGVAYKVGKFPFTANARAKANGDTDGFVKIL
SNAETGRVLGCHIIGAQAGDLIMEVVLGMEFGASSEDIARTCHAHPQLGEAVKEAALAVNKRTIHM
>Mature_465_residues
SDFDVVVIGGGPGGYVCAIRAAQLGLRVACVEKRPTLGGTCLNVGCIPSKALLQSSHLFDEANHGFAAHGITVGTLGLDM
KTMMARKDEVVEANTKGIVFLFKKNKVTHVVGSARIAGAGQVVVSGEGGEQTLTTRAIVIATGSEVTPLPGVTIDEERIV
SSTGALALAKVPKSLVVIGAGVIGLELGSVWRRLGAEVTVVEYLDHILPPMDGEVRKQSQRILEKQGLRFKLGRKVTAAE
RKGEGVSLTVEPAKGGEAETLEAETVLVAVGRRPFTDGLGLEEVGVEKTDRGFVKVDGDFQTAIEGIFAIGDVIGGMMLA
HKAEDEGMALAEMLAGESAHVNYGAIPSVVYTWPEIAGVGKTEEMLKKDGVAYKVGKFPFTANARAKANGDTDGFVKILS
NAETGRVLGCHIIGAQAGDLIMEVVLGMEFGASSEDIARTCHAHPQLGEAVKEAALAVNKRTIHM

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=463, Percent_Identity=57.8833693304536, Blast_Score=531, Evalue=1e-151,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=28.0777537796976, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI22035672, Length=489, Percent_Identity=30.2658486707566, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI148277065, Length=469, Percent_Identity=26.865671641791, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI33519430, Length=469, Percent_Identity=26.865671641791, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI33519428, Length=469, Percent_Identity=26.865671641791, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI33519426, Length=469, Percent_Identity=26.865671641791, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI291045266, Length=474, Percent_Identity=28.2700421940928, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI148277071, Length=469, Percent_Identity=26.865671641791, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI291045268, Length=468, Percent_Identity=26.9230769230769, Blast_Score=105, Evalue=7e-23,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=42.1286031042129, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=31.8181818181818, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=28.4796573875803, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=30.2961275626424, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI32565766, Length=459, Percent_Identity=59.041394335512, Blast_Score=530, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17557007, Length=488, Percent_Identity=27.4590163934426, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=388, Percent_Identity=30.9278350515464, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983429, Length=388, Percent_Identity=30.9278350515464, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=478, Percent_Identity=26.3598326359833, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=241, Percent_Identity=27.3858921161826, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=471, Percent_Identity=53.927813163482, Blast_Score=480, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=32.4894514767932, Blast_Score=240, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=28.0172413793103, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=59.5185995623632, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24640549, Length=492, Percent_Identity=30.4878048780488, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24640553, Length=492, Percent_Identity=30.4878048780488, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24640551, Length=492, Percent_Identity=30.4878048780488, Blast_Score=145, Evalue=9e-35,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=28.1947261663286, Blast_Score=130, Evalue=3e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48831; Mature: 48700

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFDVVVIGGGPGGYVCAIRAAQLGLRVACVEKRPTLGGTCLNVGCIPSKALLQSSHLF
CCCCEEEEEECCCCCEEEEEEHHHCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHH
DEANHGFAAHGITVGTLGLDMKTMMARKDEVVEANTKGIVFLFKKNKVTHVVGSARIAGA
HCCCCCEEECCEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEECCCEEEEECCCEECCC
GQVVVSGEGGEQTLTTRAIVIATGSEVTPLPGVTIDEERIVSSTGALALAKVPKSLVVIG
CEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCEECHHHHHHCCCCEEEECCCCEEEEEE
AGVIGLELGSVWRRLGAEVTVVEYLDHILPPMDGEVRKQSQRILEKQGLRFKLGRKVTAA
CCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCEEEHH
ERKGEGVSLTVEPAKGGEAETLEAETVLVAVGRRPFTDGLGLEEVGVEKTDRGFVKVDGD
HCCCCCEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCEEEECCC
FQTAIEGIFAIGDVIGGMMLAHKAEDEGMALAEMLAGESAHVNYGAIPSVVYTWPEIAGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEECHHHCCC
GKTEEMLKKDGVAYKVGKFPFTANARAKANGDTDGFVKILSNAETGRVLGCHIIGAQAGD
CCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEEEEEECCCHHH
LIMEVVLGMEFGASSEDIARTCHAHPQLGEAVKEAALAVNKRTIHM
HHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure 
SDFDVVVIGGGPGGYVCAIRAAQLGLRVACVEKRPTLGGTCLNVGCIPSKALLQSSHLF
CCCEEEEEECCCCCEEEEEEHHHCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHHHHH
DEANHGFAAHGITVGTLGLDMKTMMARKDEVVEANTKGIVFLFKKNKVTHVVGSARIAGA
HCCCCCEEECCEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEECCCEEEEECCCEECCC
GQVVVSGEGGEQTLTTRAIVIATGSEVTPLPGVTIDEERIVSSTGALALAKVPKSLVVIG
CEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCEECHHHHHHCCCCEEEECCCCEEEEEE
AGVIGLELGSVWRRLGAEVTVVEYLDHILPPMDGEVRKQSQRILEKQGLRFKLGRKVTAA
CCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEECCEEEHH
ERKGEGVSLTVEPAKGGEAETLEAETVLVAVGRRPFTDGLGLEEVGVEKTDRGFVKVDGD
HCCCCCEEEEEECCCCCCCCEEEEEEEEEEECCCCCCCCCCHHHCCCCCCCCCEEEECCC
FQTAIEGIFAIGDVIGGMMLAHKAEDEGMALAEMLAGESAHVNYGAIPSVVYTWPEIAGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCHHEEECHHHCCC
GKTEEMLKKDGVAYKVGKFPFTANARAKANGDTDGFVKILSNAETGRVLGCHIIGAQAGD
CCHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCHHHHEECCCCCCCEEEEEEECCCHHH
LIMEVVLGMEFGASSEDIARTCHAHPQLGEAVKEAALAVNKRTIHM
HHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1722146; 2914869 [H]