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The map label for this gene is pspF [H]

Identifier: 83592555

GI number: 83592555

Start: 1438898

End: 1439908

Strand: Direct

Name: pspF [H]

Synonym: Rru_A1219

Alternate gene names: 83592555

Gene position: 1438898-1439908 (Clockwise)

Preceding gene: 83592551

Following gene: 83592558

Centisome position: 33.06

GC content: 70.13

Gene sequence:

>1011_bases
ATGGACACGCTCCCTCCGATTATCGGCGAAGATCCGGCCTTTCTTGATGTCATCGAGCAGGCCTCGCGGCTGGCGCCCAT
CGACCGCCCCTGTCTGGTGGTTGGTGAACGCGGCACCGGCAAGGAACTGGTGGCGGCGCGGCTGCACTATCTGTCGCGGC
GCTGGCAGGGGCCGTTGGTCAAGGTCAATTGCGCGGCGCTGTCGGACACCCTGCTTGAAAGCGAGTTGTTCGGCCATGAG
GCGGGCGCCTTCACCGGGGCCCAGCGCCGCCATTTGGGCCGCTTCGAGCGCGCCGAGGGCGGCACCCTGATTTTGGATGA
AATCGCCAATGCCTCGCAGGCCGTTCAGGAAAAGGTGCTGCGGGTGATCGAATACGGCGAGTTGGAGCGGGTGGGCGGCG
CGGCGACGCTGCGCGTCGATGTGCGGGTGATCGGCGTCGCCAATGTCGATTTGCCAAAGCGCGCCGCCGAAGGGGGCTTC
CGGGCCGATCTTCTGGACCGGCTGGCCTTCGATGTGCTGACCCTGCCGCCCTTGCGCGCCCGCCCGGGCGATATCCTCGC
CCTGGCCGAGGCCTTCGCCCGCGCCATGGCCGGCGACTTGGAGCGTCCGGCCCCGGCCTTCTCGCCCGCCGCCCGCGCCC
AGCTTCTTGGGCATGGCTGGCCGGGTAATGTCCGCGAATTGCGCAATGCTAGCGAGCGCACCGTCGCCCGTTTGCCGCGC
GAGGCCACGGTGATCGAGACGGTGGTGATCGATCCCTTCGCTAGCCCCTGGTGTTTGGCGGCCGCCCCCGCTGCGCCGCC
GGCGATCCTTTCGCCGGCGGACGGCGCTTTGCCGCCCGAGGGCCAACCCTTCGCCGCCGCCGTCGCCGCCTATGAGCAGG
GGTTGCTACGCCGGGCCCTGGCGGCGGCCGAGGGCAATCAAGGCAAGGCCGCCCAAGCGCTGGGCCTGGGCTATCACCAG
TTCCGCCGCCTGCTCGCCCGCCATGGCCTGGGTCCGGGGGCGGAGCGGTAA

Upstream 100 bases:

>100_bases
CTTGAGAATCAAGACCAAAGCCAGTCCCTGACGCTAGGGCAAAGCGGTTCTCGCCAAAATTTAGCGGAAAGCGCTAGCGG
ATATCGCTATAATCTGGTCC

Downstream 100 bases:

>100_bases
AGACGGCGCCTTGGGGTGCGGCGGAGCGGCTTTCTCAGCGGGCGCGGGGGTGGGTGGCGCCATGCAGGCCGACCAGGGTG
CCGGCATCGACCTTGGTGTA

Product: sigma54 specific transcriptional regulator

Products: NA

Alternate protein names: Phage shock protein F [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDTLPPIIGEDPAFLDVIEQASRLAPIDRPCLVVGERGTGKELVAARLHYLSRRWQGPLVKVNCAALSDTLLESELFGHE
AGAFTGAQRRHLGRFERAEGGTLILDEIANASQAVQEKVLRVIEYGELERVGGAATLRVDVRVIGVANVDLPKRAAEGGF
RADLLDRLAFDVLTLPPLRARPGDILALAEAFARAMAGDLERPAPAFSPAARAQLLGHGWPGNVRELRNASERTVARLPR
EATVIETVVIDPFASPWCLAAAPAAPPAILSPADGALPPEGQPFAAAVAAYEQGLLRRALAAAEGNQGKAAQALGLGYHQ
FRRLLARHGLGPGAER

Sequences:

>Translated_336_residues
MDTLPPIIGEDPAFLDVIEQASRLAPIDRPCLVVGERGTGKELVAARLHYLSRRWQGPLVKVNCAALSDTLLESELFGHE
AGAFTGAQRRHLGRFERAEGGTLILDEIANASQAVQEKVLRVIEYGELERVGGAATLRVDVRVIGVANVDLPKRAAEGGF
RADLLDRLAFDVLTLPPLRARPGDILALAEAFARAMAGDLERPAPAFSPAARAQLLGHGWPGNVRELRNASERTVARLPR
EATVIETVVIDPFASPWCLAAAPAAPPAILSPADGALPPEGQPFAAAVAAYEQGLLRRALAAAEGNQGKAAQALGLGYHQ
FRRLLARHGLGPGAER
>Mature_336_residues
MDTLPPIIGEDPAFLDVIEQASRLAPIDRPCLVVGERGTGKELVAARLHYLSRRWQGPLVKVNCAALSDTLLESELFGHE
AGAFTGAQRRHLGRFERAEGGTLILDEIANASQAVQEKVLRVIEYGELERVGGAATLRVDVRVIGVANVDLPKRAAEGGF
RADLLDRLAFDVLTLPPLRARPGDILALAEAFARAMAGDLERPAPAFSPAARAQLLGHGWPGNVRELRNASERTVARLPR
EATVIETVVIDPFASPWCLAAAPAAPPAILSPADGALPPEGQPFAAAVAAYEQGLLRRALAAAEGNQGKAAQALGLGYHQ
FRRLLARHGLGPGAER

Specific function: Transcriptional activator for the phage shock protein (psp) operon (pspABCDE) and pspG gene [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87081872, Length=327, Percent_Identity=53.822629969419, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1788550, Length=332, Percent_Identity=38.2530120481928, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082117, Length=229, Percent_Identity=49.7816593886463, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1790299, Length=329, Percent_Identity=40.1215805471125, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1790437, Length=321, Percent_Identity=38.6292834890966, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=40, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1789087, Length=335, Percent_Identity=39.1044776119403, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1788905, Length=331, Percent_Identity=37.7643504531722, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789233, Length=328, Percent_Identity=35.6707317073171, Blast_Score=174, Evalue=6e-45,
Organism=Escherichia coli, GI1786524, Length=256, Percent_Identity=42.96875, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1787583, Length=232, Percent_Identity=39.6551724137931, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1789828, Length=258, Percent_Identity=34.4961240310078, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87081858, Length=225, Percent_Identity=32.8888888888889, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR014317 [H]

Pfam domain/function: PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 35733; Mature: 35733

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLPPIIGEDPAFLDVIEQASRLAPIDRPCLVVGERGTGKELVAARLHYLSRRWQGPLV
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
KVNCAALSDTLLESELFGHEAGAFTGAQRRHLGRFERAEGGTLILDEIANASQAVQEKVL
EEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHH
RVIEYGELERVGGAATLRVDVRVIGVANVDLPKRAAEGGFRADLLDRLAFDVLTLPPLRA
HHHHHCCHHHCCCEEEEEEEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCC
RPGDILALAEAFARAMAGDLERPAPAFSPAARAQLLGHGWPGNVRELRNASERTVARLPR
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCHHHHHHHHCCC
EATVIETVVIDPFASPWCLAAAPAAPPAILSPADGALPPEGQPFAAAVAAYEQGLLRRAL
HHHHEEHEEECCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAAEGNQGKAAQALGLGYHQFRRLLARHGLGPGAER
HHHCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDTLPPIIGEDPAFLDVIEQASRLAPIDRPCLVVGERGTGKELVAARLHYLSRRWQGPLV
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCEE
KVNCAALSDTLLESELFGHEAGAFTGAQRRHLGRFERAEGGTLILDEIANASQAVQEKVL
EEEHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHCCHHHHHHHHHH
RVIEYGELERVGGAATLRVDVRVIGVANVDLPKRAAEGGFRADLLDRLAFDVLTLPPLRA
HHHHHCCHHHCCCEEEEEEEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCC
RPGDILALAEAFARAMAGDLERPAPAFSPAARAQLLGHGWPGNVRELRNASERTVARLPR
CCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCHHHHHHHHCCC
EATVIETVVIDPFASPWCLAAAPAAPPAILSPADGALPPEGQPFAAAVAAYEQGLLRRAL
HHHHEEHEEECCCCCCHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AAAEGNQGKAAQALGLGYHQFRRLLARHGLGPGAER
HHHCCCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606168; 9097039; 9278503; 1712397; 7984428; 7920643 [H]