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The map label for this gene is yjeA [H]

Identifier: 83592414

GI number: 83592414

Start: 1269339

End: 1270415

Strand: Direct

Name: yjeA [H]

Synonym: Rru_A1078

Alternate gene names: 83592414

Gene position: 1269339-1270415 (Clockwise)

Preceding gene: 83592405

Following gene: 83592418

Centisome position: 29.16

GC content: 68.62

Gene sequence:

>1077_bases
ATGCCCCCGATCTCCCCCCCGTGGTTCCACCCCGACTCCCATGCGCGGCGACGGCCTTTTCTGCTGGCCCGGGCCCGGGC
GGCGGCGGCGATCCGCGCCTTTCTTGGCGAAAGCGGCTGCATCGAGGTCGATACCCCGGCGCTGCAGATCAGCCCCGGCC
TGGAACCCCATCTGCGCGCCTTCGCCACCACCTTGGAAAAGCCCTTCGATCAGGGGCGGGCGACGCTGCGGCTTCATACC
TCGCCCGAATTCGCCATGAAGAAGATCCTGGCCGCCGGCGAGACGGCGATCTTCCAACTGGCCCATGTCTTTCGCAACGG
CGAGCGCGGCCCGACCCATCATCCCGAGTTCACCATGCTTGAATGGTATCGCAGCGCGATGAGCTATGAGGCGCTGATGG
CCGAGACCGAGGATCTGGTGCGCGCCGTCGCCCGGGCGACCGGCGCCGAGCGTTTGGTGTGGGGCGACGCGGCGGCCGAT
CCCTTCGGTCCCTGGGAACGCCTGAGCGTGGCGCAAGCCCTGGAGCGGTATGCCGGCATCGATCTGCTTGGCGCCCTTGA
TGCCGGGGTGGCGATCAGCGGCAACGCGCTGGAGCCCGACGCCGGCGCCCTGGCCGAGGCCGCCCGGGCGATCGGCATCG
CCTGTTCGCCAAACGACCGCTTCGAAGACGTGTTCTTCCGCATCGTCCTTGACCGGGTCGAGCACCGCCTGGGCCACGAG
CGTCCTTGTATCCTTTACGATTATCCGGCCTGCATGGCCGCCCTGTCGCGGCGCAAGGACGACGATCCCCGGCTGGCCGA
GCGCTTCGAGGTTTATGTCGGCGGCCTGGAACTGGCCAACGCCTTTGGCGAGTTGACCGACGCCGCCGAGCAGCGCGCCC
GCTTCACCCATGACATGGACGTCAAGGAGGCGCTCTATGGCGAGCGCTATCCGATCGACGGCGACTTCCTCGAGGCCCTC
GACCAGGGCTTGGCGCCGTGCAGCGGCATCGCCCTGGGCTTCGACCGGCTGGTCATGCTGTGCACCGGGGCCGACGCCAT
CGACGATGTCCTCTGGGCGCCGGTCGCCGATCCCTGA

Upstream 100 bases:

>100_bases
GATAGTCCCGAACAAATCTCTTGCGTTTCGGAAGGGGCGGACCCTATCAGGCTTCCCCTCAGGGCGCAACTGGCCTTCCA
GCCCTTTCAGGAGTCCCCCG

Downstream 100 bases:

>100_bases
ACCCCGGTCAGCTGTTTTTGGCGGCGAAGCGGCGGCGGGCGGCGCGCCAGTCGCGGACGCTGCCGATGATGTCGCGGGCG
TTGTGGAACATCGGCACCCG

Product: tRNA synthetase, class II (D, K and N)

Products: AMP; diphosphate; L-lysyl-tRNALys [C]

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MPPISPPWFHPDSHARRRPFLLARARAAAAIRAFLGESGCIEVDTPALQISPGLEPHLRAFATTLEKPFDQGRATLRLHT
SPEFAMKKILAAGETAIFQLAHVFRNGERGPTHHPEFTMLEWYRSAMSYEALMAETEDLVRAVARATGAERLVWGDAAAD
PFGPWERLSVAQALERYAGIDLLGALDAGVAISGNALEPDAGALAEAARAIGIACSPNDRFEDVFFRIVLDRVEHRLGHE
RPCILYDYPACMAALSRRKDDDPRLAERFEVYVGGLELANAFGELTDAAEQRARFTHDMDVKEALYGERYPIDGDFLEAL
DQGLAPCSGIALGFDRLVMLCTGADAIDDVLWAPVADP

Sequences:

>Translated_358_residues
MPPISPPWFHPDSHARRRPFLLARARAAAAIRAFLGESGCIEVDTPALQISPGLEPHLRAFATTLEKPFDQGRATLRLHT
SPEFAMKKILAAGETAIFQLAHVFRNGERGPTHHPEFTMLEWYRSAMSYEALMAETEDLVRAVARATGAERLVWGDAAAD
PFGPWERLSVAQALERYAGIDLLGALDAGVAISGNALEPDAGALAEAARAIGIACSPNDRFEDVFFRIVLDRVEHRLGHE
RPCILYDYPACMAALSRRKDDDPRLAERFEVYVGGLELANAFGELTDAAEQRARFTHDMDVKEALYGERYPIDGDFLEAL
DQGLAPCSGIALGFDRLVMLCTGADAIDDVLWAPVADP
>Mature_357_residues
PPISPPWFHPDSHARRRPFLLARARAAAAIRAFLGESGCIEVDTPALQISPGLEPHLRAFATTLEKPFDQGRATLRLHTS
PEFAMKKILAAGETAIFQLAHVFRNGERGPTHHPEFTMLEWYRSAMSYEALMAETEDLVRAVARATGAERLVWGDAAADP
FGPWERLSVAQALERYAGIDLLGALDAGVAISGNALEPDAGALAEAARAIGIACSPNDRFEDVFFRIVLDRVEHRLGHER
PCILYDYPACMAALSRRKDDDPRLAERFEVYVGGLELANAFGELTDAAEQRARFTHDMDVKEALYGERYPIDGDFLEALD
QGLAPCSGIALGFDRLVMLCTGADAIDDVLWAPVADP

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=356, Percent_Identity=28.6516853932584, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI5031815, Length=356, Percent_Identity=28.6516853932584, Blast_Score=135, Evalue=7e-32,
Organism=Escherichia coli, GI87082379, Length=337, Percent_Identity=39.4658753709199, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1790571, Length=347, Percent_Identity=31.9884726224784, Blast_Score=171, Evalue=7e-44,
Organism=Escherichia coli, GI1789256, Length=347, Percent_Identity=31.700288184438, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71994340, Length=353, Percent_Identity=27.7620396600567, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17535927, Length=357, Percent_Identity=28.2913165266106, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17535925, Length=357, Percent_Identity=28.2913165266106, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6320242, Length=363, Percent_Identity=28.3746556473829, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324256, Length=357, Percent_Identity=26.890756302521, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24640851, Length=342, Percent_Identity=26.9005847953216, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640849, Length=342, Percent_Identity=26.9005847953216, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6 [C]

Molecular weight: Translated: 39254; Mature: 39123

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPISPPWFHPDSHARRRPFLLARARAAAAIRAFLGESGCIEVDTPALQISPGLEPHLRA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCHHHHH
FATTLEKPFDQGRATLRLHTSPEFAMKKILAAGETAIFQLAHVFRNGERGPTHHPEFTML
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
EWYRSAMSYEALMAETEDLVRAVARATGAERLVWGDAAADPFGPWERLSVAQALERYAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
DLLGALDAGVAISGNALEPDAGALAEAARAIGIACSPNDRFEDVFFRIVLDRVEHRLGHE
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCC
RPCILYDYPACMAALSRRKDDDPRLAERFEVYVGGLELANAFGELTDAAEQRARFTHDMD
CCEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
VKEALYGERYPIDGDFLEALDQGLAPCSGIALGFDRLVMLCTGADAIDDVLWAPVADP
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
PPISPPWFHPDSHARRRPFLLARARAAAAIRAFLGESGCIEVDTPALQISPGLEPHLRA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCEEECCCCCHHHHH
FATTLEKPFDQGRATLRLHTSPEFAMKKILAAGETAIFQLAHVFRNGERGPTHHPEFTML
HHHHHHHHHHCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
EWYRSAMSYEALMAETEDLVRAVARATGAERLVWGDAAADPFGPWERLSVAQALERYAGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCC
DLLGALDAGVAISGNALEPDAGALAEAARAIGIACSPNDRFEDVFFRIVLDRVEHRLGHE
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCC
RPCILYDYPACMAALSRRKDDDPRLAERFEVYVGGLELANAFGELTDAAEQRARFTHDMD
CCEEEEECHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCC
VKEALYGERYPIDGDFLEALDQGLAPCSGIALGFDRLVMLCTGADAIDDVLWAPVADP
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNALys [C]

Specific reaction: ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA