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The map label for this gene is puuB [H]

Identifier: 83592355

GI number: 83592355

Start: 1207986

End: 1209260

Strand: Direct

Name: puuB [H]

Synonym: Rru_A1019

Alternate gene names: 83592355

Gene position: 1207986-1209260 (Clockwise)

Preceding gene: 83592350

Following gene: 83592357

Centisome position: 27.75

GC content: 69.33

Gene sequence:

>1275_bases
ATGACCTCACGTCCCGCCAACTGGTATTCAGATACCGCAATTTCCGCGCCCGAAAGGCCCCCTCTCGCCGGTGAGGCGCG
GGCCGATGTCTGCGTCGTCGGCGCCGGCATGACCGGCAGCGCCGCCGCCTTGTTCCTGGCCGAGCGCGGCCTGTCGGTGC
GGGTGATCGAGGCCGAGAGCGTCGGCTTCGGCGCCTCGGGGCGCAGCGGCGGCCAGCTCATCGCCGGGTATGCCGCCGAG
ATGGCGACGGTTGAACGCCTGCTGCCCGAGGCACGGAGCCGCCTGTATTGGGATCTGGGGGTGGAGGCCGTCGACCTGAC
CCGCGCCCTTGTCGCCCGCCACGCCATCGCCTGCGATTTCACCGACGGCCATATCCATGCCGGTTTCAAGCCGCGCCATA
TCGGCGCGCTTGAAGAGATGATCCGCGGCTGGGAGGGGTATGGCTACCCGGGGCTGGAATGGCTTGACGGCGCGGCCATC
GGGCGGGCCGTCGCCGCCCCGGGCTATCTGGCCGGCGTGCGCGACCGCCGCGGCGGCCATCTGCACCCGCTCAATTATAC
CCTGGGATTGGCCAAGGCCGCCGAAGCCGCCGGCGCGGTCTTCCACGAATCGAGTCCCTATCTTGGCCATGAGGAAACCG
CTCAAGGGGTGGTGGTGCGCACGCCCGCCGGTTTGGTTCGGGCGCGCTGGCTGATCCTGGCCGGCAACGCCTATTTCAAG
GACCGCCCGCGCGGTCTCGCCGCGACCGTCATGCCCGTCGGCACCTATATCATCGCCACCGAGCCTTTGGGTGAGGCGCG
GGCGCGGGCGCTGATCCCCGGGGGCGAGGCGGTGGCCGATATCGCCTTCGTGCTCAATTACTACCGGCTGACCCGCGATC
ACCGCCTGCTGTTTGGCGGTGGCGTCAGTTATTCGCGCATCGATCCGCTCAGCATCCGCCGCACGCTGGGCGCCACCATG
GTCCGCACCTTCCCCCAATTGGCCGGGGTCGGGCTGTCCCATGCCTGGGGCGGCCATGTGGCGATCACCGTCAACCGGCT
GCCCCATTTCGGGCGGATCGGCAAACGCTGCCTGTTCGCCCACGGCTTTTCCGGCCATGGCGTCGGTCTGACCGGGCTGG
CGGGCAAGCTGATGGCCGAGGTGGTGGCGGGAAGCGAGGAACGCTTCGACCTGATGGCGCGCATTCCCCATCTGTCGTTT
CCCGGTGGCCGCTCCTTGCGCCTGCCGCTGCTGGTGCTGGGCTCCACCTGGCTGCGCCTGCGCGATCTGCTGTAG

Upstream 100 bases:

>100_bases
ACGCGGGCCCTTGGCAATAGTTTTTTCGCAGATGCCGCATATGGTCTTATCTCGGTAATCCCGGCATGACGGCGGCGGGC
GGGGGTGATATAGCTCGCCC

Downstream 100 bases:

>100_bases
GGCGTCCTTCGTCGGCTTCGGTCGAGAAAGCCCGGGCGGGTCAAACGAAAGACGATCCAAACGAAAACCGCCGCCCGGGG
TCCGGGCGGCGGTTTCGCGG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTSRPANWYSDTAISAPERPPLAGEARADVCVVGAGMTGSAAALFLAERGLSVRVIEAESVGFGASGRSGGQLIAGYAAE
MATVERLLPEARSRLYWDLGVEAVDLTRALVARHAIACDFTDGHIHAGFKPRHIGALEEMIRGWEGYGYPGLEWLDGAAI
GRAVAAPGYLAGVRDRRGGHLHPLNYTLGLAKAAEAAGAVFHESSPYLGHEETAQGVVVRTPAGLVRARWLILAGNAYFK
DRPRGLAATVMPVGTYIIATEPLGEARARALIPGGEAVADIAFVLNYYRLTRDHRLLFGGGVSYSRIDPLSIRRTLGATM
VRTFPQLAGVGLSHAWGGHVAITVNRLPHFGRIGKRCLFAHGFSGHGVGLTGLAGKLMAEVVAGSEERFDLMARIPHLSF
PGGRSLRLPLLVLGSTWLRLRDLL

Sequences:

>Translated_424_residues
MTSRPANWYSDTAISAPERPPLAGEARADVCVVGAGMTGSAAALFLAERGLSVRVIEAESVGFGASGRSGGQLIAGYAAE
MATVERLLPEARSRLYWDLGVEAVDLTRALVARHAIACDFTDGHIHAGFKPRHIGALEEMIRGWEGYGYPGLEWLDGAAI
GRAVAAPGYLAGVRDRRGGHLHPLNYTLGLAKAAEAAGAVFHESSPYLGHEETAQGVVVRTPAGLVRARWLILAGNAYFK
DRPRGLAATVMPVGTYIIATEPLGEARARALIPGGEAVADIAFVLNYYRLTRDHRLLFGGGVSYSRIDPLSIRRTLGATM
VRTFPQLAGVGLSHAWGGHVAITVNRLPHFGRIGKRCLFAHGFSGHGVGLTGLAGKLMAEVVAGSEERFDLMARIPHLSF
PGGRSLRLPLLVLGSTWLRLRDLL
>Mature_423_residues
TSRPANWYSDTAISAPERPPLAGEARADVCVVGAGMTGSAAALFLAERGLSVRVIEAESVGFGASGRSGGQLIAGYAAEM
ATVERLLPEARSRLYWDLGVEAVDLTRALVARHAIACDFTDGHIHAGFKPRHIGALEEMIRGWEGYGYPGLEWLDGAAIG
RAVAAPGYLAGVRDRRGGHLHPLNYTLGLAKAAEAAGAVFHESSPYLGHEETAQGVVVRTPAGLVRARWLILAGNAYFKD
RPRGLAATVMPVGTYIIATEPLGEARARALIPGGEAVADIAFVLNYYRLTRDHRLLFGGGVSYSRIDPLSIRRTLGATMV
RTFPQLAGVGLSHAWGGHVAITVNRLPHFGRIGKRCLFAHGFSGHGVGLTGLAGKLMAEVVAGSEERFDLMARIPHLSFP
GGRSLRLPLLVLGSTWLRLRDLL

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=425, Percent_Identity=41.4117647058824, Blast_Score=336, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45175; Mature: 45043

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRPANWYSDTAISAPERPPLAGEARADVCVVGAGMTGSAAALFLAERGLSVRVIEAES
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECC
VGFGASGRSGGQLIAGYAAEMATVERLLPEARSRLYWDLGVEAVDLTRALVARHAIACDF
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHEEEEC
TDGHIHAGFKPRHIGALEEMIRGWEGYGYPGLEWLDGAAIGRAVAAPGYLAGVRDRRGGH
CCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHCCCCHHCCCCCCCCCC
LHPLNYTLGLAKAAEAAGAVFHESSPYLGHEETAQGVVVRTPAGLVRARWLILAGNAYFK
CCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHCCCEEEECCCHHHHEEEEEEECCCEEC
DRPRGLAATVMPVGTYIIATEPLGEARARALIPGGEAVADIAFVLNYYRLTRDHRLLFGG
CCCCCCEEEEEECCEEEEEECCCCHHHHEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEC
GVSYSRIDPLSIRRTLGATMVRTFPQLAGVGLSHAWGGHVAITVNRLPHFGRIGKRCLFA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCEEEE
HGFSGHGVGLTGLAGKLMAEVVAGSEERFDLMARIPHLSFPGGRSLRLPLLVLGSTWLRL
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEECCCEEECHHHHHH
RDLL
HHCC
>Mature Secondary Structure 
TSRPANWYSDTAISAPERPPLAGEARADVCVVGAGMTGSAAALFLAERGLSVRVIEAES
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCEEEEEEECC
VGFGASGRSGGQLIAGYAAEMATVERLLPEARSRLYWDLGVEAVDLTRALVARHAIACDF
CCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHHHHHHHEEEEC
TDGHIHAGFKPRHIGALEEMIRGWEGYGYPGLEWLDGAAIGRAVAAPGYLAGVRDRRGGH
CCCCEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHCCCCHHCCCCCCCCCC
LHPLNYTLGLAKAAEAAGAVFHESSPYLGHEETAQGVVVRTPAGLVRARWLILAGNAYFK
CCCCCHHHHHHHHHHHHHHEEECCCCCCCCCHHCCCEEEECCCHHHHEEEEEEECCCEEC
DRPRGLAATVMPVGTYIIATEPLGEARARALIPGGEAVADIAFVLNYYRLTRDHRLLFGG
CCCCCCEEEEEECCEEEEEECCCCHHHHEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEC
GVSYSRIDPLSIRRTLGATMVRTFPQLAGVGLSHAWGGHVAITVNRLPHFGRIGKRCLFA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHCCEEEE
HGFSGHGVGLTGLAGKLMAEVVAGSEERFDLMARIPHLSFPGGRSLRLPLLVLGSTWLRL
CCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEECCCEEECHHHHHH
RDLL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]