Click here to switch to the map view.

The map label for this gene is rpoH [H]

Identifier: 83592357

GI number: 83592357

Start: 1210454

End: 1211374

Strand: Direct

Name: rpoH [H]

Synonym: Rru_A1021

Alternate gene names: 83592357

Gene position: 1210454-1211374 (Clockwise)

Preceding gene: 83592355

Following gene: 83592360

Centisome position: 27.81

GC content: 62.0

Gene sequence:

>921_bases
ATGAGTACCTATCGATACAAATTGCCCGCGGTGTCCGACGAAGGTGGCCTGTCGCGCTATCTGACCGAGATCAAGCGCTT
TCCGATGCTGACTCCGGAAGAAGAGTTCATGCTGGCCAAGCGTCTGCAAGACGAGGGGGATCGCGACGCGGCCCACCGTC
TGGTCACCAGCCATCTGCGTTTGGTCGCCAAAATCGCCATGGGCTATCGCCACTACGGTCTGCCGGTATCGGATCTGGTC
GCCGAAGGCAACATGGGCCTGATGAAGGCCGTCAAGAAATTCGAGGCCGATCGCGGCTTCCGGCTGGCCACCTATGCCAT
GTGGTGGATCAAGGCCAGCATCAACGAATACATCTTGAACTCCTGGTCGCTGGTCAAGGTCGGCACGGTCGCCGCCCAGA
AAAAGCTGTTCTTCAATCTGCGTCGTCTGAAGGCCAAGCTTGGCGTCTATGAGGAAGGCGATCTGTCGCCCGAAGACGTC
AAGACCATCGCCACCCAGTTGAAGGTGACGGAAGCCGACGTGGTGATGATGAACCGCCGTCTGGCCGGTCCCGACGCCTC
GCTCAACGTTCCGGTGGGCGAAGACCAGTCGATGGATCGCATGGATCTGTTGATGGACGAGGGCGATAACCAGGAAGAGG
TTTACGCCGCCCACGAGGAGCAGAGTCTGGGCAATCGCCTGCTCAAGGACGCCTTGTCGACCTTGACCGAGCGCGAGCGT
CACATCATCACCGCGCGCCGCCTGCGCAGCGAGCCGGTGACCCTGGAGGAACTGGGCGGCGAATACGGCGTCAGCCGCGA
ACGCGTGCGTCAGATCGAGAACCGGGCCTTCGAGAAGCTGCAGACCGCGGTGCGCAAGGCGGCCCGGGCGCTCAACGATC
GCCAGCCCCATCACGGCGGGGTCGAACCCGCCATGGCCTGA

Upstream 100 bases:

>100_bases
TTCCGAGAAAAGAATGAATCCAAGGGTGCGAGACCCGGGAACCGGCTTCAACCCGCCGCGTTTCCTGGGTAGGCCCGACG
CGAAAGGAGTGGCAACCGTC

Downstream 100 bases:

>100_bases
CGGCGGGGGCCACCCCGCCGGCCGCTGTCCTTTCGCCAATCGCGTCGCCTTAAGAAAAAGGGTGGGGAGCAAACGCTTCC
CACCCTTTTTTTGACTGATC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSTYRYKLPAVSDEGGLSRYLTEIKRFPMLTPEEEFMLAKRLQDEGDRDAAHRLVTSHLRLVAKIAMGYRHYGLPVSDLV
AEGNMGLMKAVKKFEADRGFRLATYAMWWIKASINEYILNSWSLVKVGTVAAQKKLFFNLRRLKAKLGVYEEGDLSPEDV
KTIATQLKVTEADVVMMNRRLAGPDASLNVPVGEDQSMDRMDLLMDEGDNQEEVYAAHEEQSLGNRLLKDALSTLTERER
HIITARRLRSEPVTLEELGGEYGVSRERVRQIENRAFEKLQTAVRKAARALNDRQPHHGGVEPAMA

Sequences:

>Translated_306_residues
MSTYRYKLPAVSDEGGLSRYLTEIKRFPMLTPEEEFMLAKRLQDEGDRDAAHRLVTSHLRLVAKIAMGYRHYGLPVSDLV
AEGNMGLMKAVKKFEADRGFRLATYAMWWIKASINEYILNSWSLVKVGTVAAQKKLFFNLRRLKAKLGVYEEGDLSPEDV
KTIATQLKVTEADVVMMNRRLAGPDASLNVPVGEDQSMDRMDLLMDEGDNQEEVYAAHEEQSLGNRLLKDALSTLTERER
HIITARRLRSEPVTLEELGGEYGVSRERVRQIENRAFEKLQTAVRKAARALNDRQPHHGGVEPAMA
>Mature_305_residues
STYRYKLPAVSDEGGLSRYLTEIKRFPMLTPEEEFMLAKRLQDEGDRDAAHRLVTSHLRLVAKIAMGYRHYGLPVSDLVA
EGNMGLMKAVKKFEADRGFRLATYAMWWIKASINEYILNSWSLVKVGTVAAQKKLFFNLRRLKAKLGVYEEGDLSPEDVK
TIATQLKVTEADVVMMNRRLAGPDASLNVPVGEDQSMDRMDLLMDEGDNQEEVYAAHEEQSLGNRLLKDALSTLTERERH
IITARRLRSEPVTLEELGGEYGVSRERVRQIENRAFEKLQTAVRKAARALNDRQPHHGGVEPAMA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=42.1428571428571, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1789098, Length=268, Percent_Identity=37.6865671641791, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1789448, Length=252, Percent_Identity=32.1428571428571, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34706; Mature: 34575

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYRYKLPAVSDEGGLSRYLTEIKRFPMLTPEEEFMLAKRLQDEGDRDAAHRLVTSHLR
CCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LVAKIAMGYRHYGLPVSDLVAEGNMGLMKAVKKFEADRGFRLATYAMWWIKASINEYILN
HHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHC
SWSLVKVGTVAAQKKLFFNLRRLKAKLGVYEEGDLSPEDVKTIATQLKVTEADVVMMNRR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LAGPDASLNVPVGEDQSMDRMDLLMDEGDNQEEVYAAHEEQSLGNRLLKDALSTLTERER
CCCCCCCEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIITARRLRSEPVTLEELGGEYGVSRERVRQIENRAFEKLQTAVRKAARALNDRQPHHGG
HHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VEPAMA
CCCCCC
>Mature Secondary Structure 
STYRYKLPAVSDEGGLSRYLTEIKRFPMLTPEEEFMLAKRLQDEGDRDAAHRLVTSHLR
CCCEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHH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CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]