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The map label for this gene is potH [H]

Identifier: 83592352

GI number: 83592352

Start: 1204389

End: 1205381

Strand: Reverse

Name: potH [H]

Synonym: Rru_A1016

Alternate gene names: 83592352

Gene position: 1205381-1204389 (Counterclockwise)

Preceding gene: 83592353

Following gene: 83592351

Centisome position: 27.69

GC content: 63.24

Gene sequence:

>993_bases
ATGACCGCCGAGCCGGCCCCGGGCGCCGCGCCGGTGTCGCGCCCGCCCGAAATCCTGCCGGGCATGGCGCCTTGGCGCAT
GTGGCTTGATGCCCAGATCCGCCACAACGGCCGGCGGCTGGTGATCCTGGTGCCCTATGTCTGGCTGCTGCTGTTTTTCG
TTCTGCCCTTCGCCATCGTCCTCAAGATCTCCTTTGCCGAATTCCGCGTCGGCGTGCCGCCCTATACGGCCCTGATCGAC
TTCACCCAGGACGCCTATCTGCGGCTGACCCTGGCGCTGTCGAACTACAAGTACATGCTCGACGACGAGATGTACGTGAT
CGCCTATCTGAATTCGCTGCGCATCGCCGGCATTTCCACCGTGCTCGCCCTGCTGATCGGCTATCCGATGGCCTATGGCA
TCGCCAGGGCGCCCAAGGGGCCGCGCACCATCTTGCTGTTCCTGGTGATCTTGCCGTTCTGGACGTCGTTCCTCATCCGC
GTCTATGCCTGGATGGGCATCCTCAAGGGCAACGGCCTGCTCAATACCTTCCTGATCTGGACCGGGGTGATCGACCAGCC
GCTGACCATCATGGGCACGGAATGGGCGGTGTTCATCGGCATCGTCTATTCCTATCTGCCCTTCATGGTGCTGCCGCTGT
ACGCCACCTTGGAGAAGCTCGACCCCAGCTTGCTCGAAGCCGCCCACGACCTGGGCTGCCGGCCGTGGAAGGCCTTTCTG
ACCATCACCCTGCCGCTGTCGGTGCCGGGAATCATCGCCGGATCGATGCTGGTGTTCATCCCCTCGGTCGGCGAGTTCGT
CATCCCCGAACTGCTGGGCGGGGCCGATACCTTCATGATCGGCCGGGTGCTGTGGAACGAGTTCTTCCTCAACCGCGATT
GGCCGGTGGCCTCGGCGGTGGCGGTGGCGATGCTGCTGATGCTGGTGGTGCCGATCATGATCTTCCAGAACATCCAGGCC
AAACAGGCCGAAGCGGCGGAGGAACGGTCATAA

Upstream 100 bases:

>100_bases
TGACCGTCGCCAACCATGAACGCGATGTGGAATGGGAGATCACCTGGGGCGACCGCGTCTATGTCAGTTGGGCGGCGACC
AGCGCCGTGGTGGTGTCGTC

Downstream 100 bases:

>100_bases
TGCGCCGCAACTGGTTCTTGCTGCTGACCATGGGCTTCGGCTTCGCCTTCTTGTATGTGCCGATCCTGTTGCTGATGATC
TATTCGTTCAACGAAAGCCG

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTAEPAPGAAPVSRPPEILPGMAPWRMWLDAQIRHNGRRLVILVPYVWLLLFFVLPFAIVLKISFAEFRVGVPPYTALID
FTQDAYLRLTLALSNYKYMLDDEMYVIAYLNSLRIAGISTVLALLIGYPMAYGIARAPKGPRTILLFLVILPFWTSFLIR
VYAWMGILKGNGLLNTFLIWTGVIDQPLTIMGTEWAVFIGIVYSYLPFMVLPLYATLEKLDPSLLEAAHDLGCRPWKAFL
TITLPLSVPGIIAGSMLVFIPSVGEFVIPELLGGADTFMIGRVLWNEFFLNRDWPVASAVAVAMLLMLVVPIMIFQNIQA
KQAEAAEERS

Sequences:

>Translated_330_residues
MTAEPAPGAAPVSRPPEILPGMAPWRMWLDAQIRHNGRRLVILVPYVWLLLFFVLPFAIVLKISFAEFRVGVPPYTALID
FTQDAYLRLTLALSNYKYMLDDEMYVIAYLNSLRIAGISTVLALLIGYPMAYGIARAPKGPRTILLFLVILPFWTSFLIR
VYAWMGILKGNGLLNTFLIWTGVIDQPLTIMGTEWAVFIGIVYSYLPFMVLPLYATLEKLDPSLLEAAHDLGCRPWKAFL
TITLPLSVPGIIAGSMLVFIPSVGEFVIPELLGGADTFMIGRVLWNEFFLNRDWPVASAVAVAMLLMLVVPIMIFQNIQA
KQAEAAEERS
>Mature_329_residues
TAEPAPGAAPVSRPPEILPGMAPWRMWLDAQIRHNGRRLVILVPYVWLLLFFVLPFAIVLKISFAEFRVGVPPYTALIDF
TQDAYLRLTLALSNYKYMLDDEMYVIAYLNSLRIAGISTVLALLIGYPMAYGIARAPKGPRTILLFLVILPFWTSFLIRV
YAWMGILKGNGLLNTFLIWTGVIDQPLTIMGTEWAVFIGIVYSYLPFMVLPLYATLEKLDPSLLEAAHDLGCRPWKAFLT
ITLPLSVPGIIAGSMLVFIPSVGEFVIPELLGGADTFMIGRVLWNEFFLNRDWPVASAVAVAMLLMLVVPIMIFQNIQAK
QAEAAEERS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=321, Percent_Identity=59.8130841121495, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1787369, Length=282, Percent_Identity=40.4255319148936, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787713, Length=258, Percent_Identity=31.7829457364341, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1788764, Length=241, Percent_Identity=26.1410788381743, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786980, Length=71, Percent_Identity=47.887323943662, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36885; Mature: 36753

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPAPGAAPVSRPPEILPGMAPWRMWLDAQIRHNGRRLVILVPYVWLLLFFVLPFAIV
CCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
LKISFAEFRVGVPPYTALIDFTQDAYLRLTLALSNYKYMLDDEMYVIAYLNSLRIAGIST
HHHHHHHHCCCCCCHHHHHCCCCCCEEEEEEEECCCEEEECCCEEEEEEHHHHHHHHHHH
VLALLIGYPMAYGIARAPKGPRTILLFLVILPFWTSFLIRVYAWMGILKGNGLLNTFLIW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TGVIDQPLTIMGTEWAVFIGIVYSYLPFMVLPLYATLEKLDPSLLEAAHDLGCRPWKAFL
HHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
TITLPLSVPGIIAGSMLVFIPSVGEFVIPELLGGADTFMIGRVLWNEFFLNRDWPVASAV
EEEECCCCCHHHHHHHHHEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHH
AVAMLLMLVVPIMIFQNIQAKQAEAAEERS
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
TAEPAPGAAPVSRPPEILPGMAPWRMWLDAQIRHNGRRLVILVPYVWLLLFFVLPFAIV
CCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHH
LKISFAEFRVGVPPYTALIDFTQDAYLRLTLALSNYKYMLDDEMYVIAYLNSLRIAGIST
HHHHHHHHCCCCCCHHHHHCCCCCCEEEEEEEECCCEEEECCCEEEEEEHHHHHHHHHHH
VLALLIGYPMAYGIARAPKGPRTILLFLVILPFWTSFLIRVYAWMGILKGNGLLNTFLIW
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
TGVIDQPLTIMGTEWAVFIGIVYSYLPFMVLPLYATLEKLDPSLLEAAHDLGCRPWKAFL
HHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEE
TITLPLSVPGIIAGSMLVFIPSVGEFVIPELLGGADTFMIGRVLWNEFFLNRDWPVASAV
EEEECCCCCHHHHHHHHHEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHH
AVAMLLMLVVPIMIFQNIQAKQAEAAEERS
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]