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The map label for this gene is nifK1 [H]

Identifier: 83592348

GI number: 83592348

Start: 1199508

End: 1201040

Strand: Direct

Name: nifK1 [H]

Synonym: Rru_A1012

Alternate gene names: 83592348

Gene position: 1199508-1201040 (Clockwise)

Preceding gene: 83592347

Following gene: 83592349

Centisome position: 27.56

GC content: 61.06

Gene sequence:

>1533_bases
ATGCCCCAGAACGCGGACCTCATCAAAGACCACATTCCCTTGTTCCGGGAACCCGAATACAAAGAAATGTTCGCGCGCAA
GCGTGAGTGTTTCGAAAACAGCCATGGCGAGGCCAAGGTCGCCGAAGTCGCCGAATGGACCAAGTCCTGGGACTACCGGG
AAAAGAACCTGGCCCGGGAAGCCCTGGTGATCAATCCCAACAAGGCTTGCCAGCCGCTGGGCGCCCTGTTCGCCGCCGCC
GGTTTCGACGGCACGCTGGGCTATGTCCATGGCTCGCAGGGCTGCGCCGCCTATTTCCGCTCGCATCTGTCGCGTCATTT
CAAGGAAGCCTCCTCGGTGGTTTCCGACAGCATGACCGAAGACGCGGCGGTGTTCGGCGGGCTCAACAACATGGTCGATG
GCCTCGCCAACGCCTATGCCCTGTACAAGCCCAAGATGATCGCCGTCTGCACCTCGTGCATGGCCGAGGTCATCGGCGAC
GACATCAGCTCGTTCATCGGCAATGCCAAGACCAAGGAGTCGATCCCGGCCGACTTCCCGGTGCCCTTCGCCCACACCCC
GTCGTTCGTCGGCAGCCACATCGTCGGCTACGACAATATGATGAAGGGTATCCTGACGACGTTTTGGGACGGCAAGGTCC
GCGACGCCGCCACCGACAAGATCAACATCATTCCCGGCTTCGACGGTTACAGCGTCGGCAATATTCGGGAACTGAAGCGG
ATCCTTGGCATGATCGGCGTCGAGTACACCGTTTTGTCGGATACCTCGGACGTTTACGATACGCCGACCGACGGTGAATT
CCGCATGTATGACGGCGGCACCACCCAGGACGACACCATCGCCGCCCTGCAGGCCAAGGCCACGCTGTCGCTGTCGGAAT
ATTGCACCCCGGCGACGCTGAAGTTCATCGCCGAGACCGGTCAGGAAACCGCCGCCTTCAACTACCCGATGGGCGTGGGC
GCCACCGATGATTTCCTGGTCAAGCTGGTCGCCCTGACCGGCAAGGACATCCCCGAGGCGCTGACCAAGGAACGCGGTCG
CCTTGTCGATGCCCTGGCCGACAGCATGACTTGGCTGCACGGCAAGAAGATGGCCGTTTACGGCGATCCCGACTTCACCT
TGTCGATGGTCCGCTTCCTGCTGGAAATGGGCGTCGAGCCGGTCCACGTGCTGGCCACCAACGGCACCAAGAAATGGGAA
AAGCAGGTCAAGGCCCTGCTCGCCACCAGCCCGTTTGGCAAGGACGCCCAGGTTTGGCAGGGCAAGGACCTTTGGCACCT
GCGCTCGCTGCTGGCCACCGAGCCCGCCGATTTCCTGATCGGCAACTCCTATGGCAAGTATCTGGAACTGGATCTGGGGA
TCCCCTTGATCCGCCTGACCTTCCCGATTTTCGACCGTCACCACCACCACCGCTTCCCGACCATGTTCTATGACGGGTCG
CTGCGCGTGCTGACGACGATCCTCGATAAGGTTTTCGATACCCTGGATCGCGAGAGCGGCGAAAACGGCATCAGCTTCGA
TCTGACCCGCTAA

Upstream 100 bases:

>100_bases
GCCATCAACAATCCGGTCTGGGCCTTGCTGAAAGCCCCGTGGACCAAGGCCGCCGCCGAGTAAGTCCCTTAAGACCAGAA
CGCCAGACTGGAGAGAGCTG

Downstream 100 bases:

>100_bases
CATCGGTCTCATCGCCAAGGCTCTTATCGGTGGACGCGGTCCAAGCCCCATCCTTGGTCGCGTCCCTCCTTCCGCCGTCC
CCGGGGTCCATCCCGTGGAC

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MPQNADLIKDHIPLFREPEYKEMFARKRECFENSHGEAKVAEVAEWTKSWDYREKNLAREALVINPNKACQPLGALFAAA
GFDGTLGYVHGSQGCAAYFRSHLSRHFKEASSVVSDSMTEDAAVFGGLNNMVDGLANAYALYKPKMIAVCTSCMAEVIGD
DISSFIGNAKTKESIPADFPVPFAHTPSFVGSHIVGYDNMMKGILTTFWDGKVRDAATDKINIIPGFDGYSVGNIRELKR
ILGMIGVEYTVLSDTSDVYDTPTDGEFRMYDGGTTQDDTIAALQAKATLSLSEYCTPATLKFIAETGQETAAFNYPMGVG
ATDDFLVKLVALTGKDIPEALTKERGRLVDALADSMTWLHGKKMAVYGDPDFTLSMVRFLLEMGVEPVHVLATNGTKKWE
KQVKALLATSPFGKDAQVWQGKDLWHLRSLLATEPADFLIGNSYGKYLELDLGIPLIRLTFPIFDRHHHHRFPTMFYDGS
LRVLTTILDKVFDTLDRESGENGISFDLTR

Sequences:

>Translated_510_residues
MPQNADLIKDHIPLFREPEYKEMFARKRECFENSHGEAKVAEVAEWTKSWDYREKNLAREALVINPNKACQPLGALFAAA
GFDGTLGYVHGSQGCAAYFRSHLSRHFKEASSVVSDSMTEDAAVFGGLNNMVDGLANAYALYKPKMIAVCTSCMAEVIGD
DISSFIGNAKTKESIPADFPVPFAHTPSFVGSHIVGYDNMMKGILTTFWDGKVRDAATDKINIIPGFDGYSVGNIRELKR
ILGMIGVEYTVLSDTSDVYDTPTDGEFRMYDGGTTQDDTIAALQAKATLSLSEYCTPATLKFIAETGQETAAFNYPMGVG
ATDDFLVKLVALTGKDIPEALTKERGRLVDALADSMTWLHGKKMAVYGDPDFTLSMVRFLLEMGVEPVHVLATNGTKKWE
KQVKALLATSPFGKDAQVWQGKDLWHLRSLLATEPADFLIGNSYGKYLELDLGIPLIRLTFPIFDRHHHHRFPTMFYDGS
LRVLTTILDKVFDTLDRESGENGISFDLTR
>Mature_509_residues
PQNADLIKDHIPLFREPEYKEMFARKRECFENSHGEAKVAEVAEWTKSWDYREKNLAREALVINPNKACQPLGALFAAAG
FDGTLGYVHGSQGCAAYFRSHLSRHFKEASSVVSDSMTEDAAVFGGLNNMVDGLANAYALYKPKMIAVCTSCMAEVIGDD
ISSFIGNAKTKESIPADFPVPFAHTPSFVGSHIVGYDNMMKGILTTFWDGKVRDAATDKINIIPGFDGYSVGNIRELKRI
LGMIGVEYTVLSDTSDVYDTPTDGEFRMYDGGTTQDDTIAALQAKATLSLSEYCTPATLKFIAETGQETAAFNYPMGVGA
TDDFLVKLVALTGKDIPEALTKERGRLVDALADSMTWLHGKKMAVYGDPDFTLSMVRFLLEMGVEPVHVLATNGTKKWEK
QVKALLATSPFGKDAQVWQGKDLWHLRSLLATEPADFLIGNSYGKYLELDLGIPLIRLTFPIFDRHHHHRFPTMFYDGSL
RVLTTILDKVFDTLDRESGENGISFDLTR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56435; Mature: 56304

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNADLIKDHIPLFREPEYKEMFARKRECFENSHGEAKVAEVAEWTKSWDYREKNLARE
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCC
ALVINPNKACQPLGALFAAAGFDGTLGYVHGSQGCAAYFRSHLSRHFKEASSVVSDSMTE
EEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAVFGGLNNMVDGLANAYALYKPKMIAVCTSCMAEVIGDDISSFIGNAKTKESIPADFP
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VPFAHTPSFVGSHIVGYDNMMKGILTTFWDGKVRDAATDKINIIPGFDGYSVGNIRELKR
CCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHH
ILGMIGVEYTVLSDTSDVYDTPTDGEFRMYDGGTTQDDTIAALQAKATLSLSEYCTPATL
HHHHHCCEEEEECCCCHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHCCHHHH
KFIAETGQETAAFNYPMGVGATDDFLVKLVALTGKDIPEALTKERGRLVDALADSMTWLH
HHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GKKMAVYGDPDFTLSMVRFLLEMGVEPVHVLATNGTKKWEKQVKALLATSPFGKDAQVWQ
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
GKDLWHLRSLLATEPADFLIGNSYGKYLELDLGIPLIRLTFPIFDRHHHHRFPTMFYDGS
CCCHHHHHHHHHCCCCHHEEECCCCCEEEEECCCCEEHHHHHHHHCCCCCCCCCEEECCC
LRVLTTILDKVFDTLDRESGENGISFDLTR
HHHHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
PQNADLIKDHIPLFREPEYKEMFARKRECFENSHGEAKVAEVAEWTKSWDYREKNLARE
CCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCC
ALVINPNKACQPLGALFAAAGFDGTLGYVHGSQGCAAYFRSHLSRHFKEASSVVSDSMTE
EEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAAVFGGLNNMVDGLANAYALYKPKMIAVCTSCMAEVIGDDISSFIGNAKTKESIPADFP
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
VPFAHTPSFVGSHIVGYDNMMKGILTTFWDGKVRDAATDKINIIPGFDGYSVGNIRELKR
CCCCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHH
ILGMIGVEYTVLSDTSDVYDTPTDGEFRMYDGGTTQDDTIAALQAKATLSLSEYCTPATL
HHHHHCCEEEEECCCCHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHCCHHHH
KFIAETGQETAAFNYPMGVGATDDFLVKLVALTGKDIPEALTKERGRLVDALADSMTWLH
HHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GKKMAVYGDPDFTLSMVRFLLEMGVEPVHVLATNGTKKWEKQVKALLATSPFGKDAQVWQ
CCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
GKDLWHLRSLLATEPADFLIGNSYGKYLELDLGIPLIRLTFPIFDRHHHHRFPTMFYDGS
CCCHHHHHHHHHCCCCHHEEECCCCCEEEEECCCCEEHHHHHHHHCCCCCCCCCEEECCC
LRVLTTILDKVFDTLDRESGENGISFDLTR
HHHHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]