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The map label for this gene is nifD1 [H]

Identifier: 83592347

GI number: 83592347

Start: 1198007

End: 1199470

Strand: Direct

Name: nifD1 [H]

Synonym: Rru_A1011

Alternate gene names: 83592347

Gene position: 1198007-1199470 (Clockwise)

Preceding gene: 83592346

Following gene: 83592348

Centisome position: 27.52

GC content: 60.52

Gene sequence:

>1464_bases
ATGAGCCTCGAATACACCAACGATGCGGAATTGAGCCAGAAGGCGATCGAGGAGGTCCTGGAGGCCTATCCCGAAAAGGC
CGCGAAGAAGCGCAAAAAGCACCTTGGCACCATCGTTGCCGAGGGCGAGGGCAGCTCTTGCGGGGTGAAGTCCAACGTCA
AGGCCATTCCGGGCGTCATGACCATCCGCGGCTGCGCCTATGCCGGCTCGAAGGGCGTGGTCTGGGGTCCGGTCAAGGAC
ATGGTCCACATCAGTCACGGCCCGGTCGGCTGCGGTCAGTACTCCTGGTCCCAGCGCCGCAATTACTTCACCGGTCAGGT
GGGCGTCGATTCTTTCGTCACCATGCAGTTCACCTCGGATTTCCAGGAAAAAGACATCGTCTTTGGCGGTGACAAGAAGC
TGGAAAAGGTGATCGACGAGATCAAGGGGCTGTTTCCGCTGGTTCGCGGCATCAGCATCCAGTCCGAATGCCCGATCGGC
CTGATCGGCGACGATATCGAAGCCGTCGCCCGCAAGAAGGCCAAGGATGTCGGCTTGCCGATCATCCCGGTGCGCTGCGA
AGGCTTCCGCGGCGTGTCGCAGTCGCTTGGTCACCATATCGCCAATGACGCCATCCGCGACTGGGTCTTCTCGCGCGACA
GCGAAAGCGCCTTCGAGACCACGCCCTATGACGTCAACATCATCGGCGATTACAACATCGGTGGCGACGCCTGGGCCTCG
CGCATTCTGTTGGAGGAAATGGGCCTGCGGGTGATCGCCCAATGGTCGGGCGATGCCACCATCGCCGAAATGGAACGCGC
CCCCAAGGCCAAGCTGAACCTCATCCATTGCTACCGGTCGATGAATTACATCTGCCGCCACATGGAAGAGAAGCACGGCG
TGCCCTGGATGGAATACAACTTCTTCGGTCCCTCGCAGATCGAGAAGTCGTTGCGCGCCATCGCCGCCAATTTCGACGAG
ACCATCCAGAAGAAGGCCGAGGAGGTGATCGCCGCCCATCGCCCGACGGTCGACGCGGTGATCAACAAGTACAAGGCCCG
CCTCGAAGGCAAGCGCGTCATGCTGTATGTCGGCGGCCTGCGCCCCCGTCACGTGATGACCGCTTATGAAGACCTCGGCA
TGCAGATCTGCGGCGCCGGTTATGAATTCGCCCATAGCGACGATTACCAGCGCACCACCGAATACGCCAAGGAAGGCACG
CTGATCTATGACGACCTGACCGGCTACGAGCTGGAGCGGTTCATCGAGAAGCTGCGCCCCGATCTGGTGGGCTCGGGCAT
CAAGGAAAAATACGCCGTTCAGAAGATGGGCGTGCCTTTCCGCCAGATGCACTCCTGGGATTACTCGGGTCCTTACCACG
GCTATGACGGCTTCGCCATCTTCGCCCGTGACATGGACATGGCCATCAACAATCCGGTCTGGGCCTTGCTGAAAGCCCCG
TGGACCAAGGCCGCCGCCGAGTAA

Upstream 100 bases:

>100_bases
TCCCCCCGGCCGGCCGGTCAGGCCGCCAGGGGCGACGGAAGCGGGCGGACCTCCAAGAAGGCCTCTTCGCCGAGGCGATA
GTCGATCAGAGGAGAGGGAT

Downstream 100 bases:

>100_bases
GTCCCTTAAGACCAGAACGCCAGACTGGAGAGAGCTGATGCCCCAGAACGCGGACCTCATCAAAGACCACATTCCCTTGT
TCCGGGAACCCGAATACAAA

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSLEYTNDAELSQKAIEEVLEAYPEKAAKKRKKHLGTIVAEGEGSSCGVKSNVKAIPGVMTIRGCAYAGSKGVVWGPVKD
MVHISHGPVGCGQYSWSQRRNYFTGQVGVDSFVTMQFTSDFQEKDIVFGGDKKLEKVIDEIKGLFPLVRGISIQSECPIG
LIGDDIEAVARKKAKDVGLPIIPVRCEGFRGVSQSLGHHIANDAIRDWVFSRDSESAFETTPYDVNIIGDYNIGGDAWAS
RILLEEMGLRVIAQWSGDATIAEMERAPKAKLNLIHCYRSMNYICRHMEEKHGVPWMEYNFFGPSQIEKSLRAIAANFDE
TIQKKAEEVIAAHRPTVDAVINKYKARLEGKRVMLYVGGLRPRHVMTAYEDLGMQICGAGYEFAHSDDYQRTTEYAKEGT
LIYDDLTGYELERFIEKLRPDLVGSGIKEKYAVQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINNPVWALLKAP
WTKAAAE

Sequences:

>Translated_487_residues
MSLEYTNDAELSQKAIEEVLEAYPEKAAKKRKKHLGTIVAEGEGSSCGVKSNVKAIPGVMTIRGCAYAGSKGVVWGPVKD
MVHISHGPVGCGQYSWSQRRNYFTGQVGVDSFVTMQFTSDFQEKDIVFGGDKKLEKVIDEIKGLFPLVRGISIQSECPIG
LIGDDIEAVARKKAKDVGLPIIPVRCEGFRGVSQSLGHHIANDAIRDWVFSRDSESAFETTPYDVNIIGDYNIGGDAWAS
RILLEEMGLRVIAQWSGDATIAEMERAPKAKLNLIHCYRSMNYICRHMEEKHGVPWMEYNFFGPSQIEKSLRAIAANFDE
TIQKKAEEVIAAHRPTVDAVINKYKARLEGKRVMLYVGGLRPRHVMTAYEDLGMQICGAGYEFAHSDDYQRTTEYAKEGT
LIYDDLTGYELERFIEKLRPDLVGSGIKEKYAVQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINNPVWALLKAP
WTKAAAE
>Mature_486_residues
SLEYTNDAELSQKAIEEVLEAYPEKAAKKRKKHLGTIVAEGEGSSCGVKSNVKAIPGVMTIRGCAYAGSKGVVWGPVKDM
VHISHGPVGCGQYSWSQRRNYFTGQVGVDSFVTMQFTSDFQEKDIVFGGDKKLEKVIDEIKGLFPLVRGISIQSECPIGL
IGDDIEAVARKKAKDVGLPIIPVRCEGFRGVSQSLGHHIANDAIRDWVFSRDSESAFETTPYDVNIIGDYNIGGDAWASR
ILLEEMGLRVIAQWSGDATIAEMERAPKAKLNLIHCYRSMNYICRHMEEKHGVPWMEYNFFGPSQIEKSLRAIAANFDET
IQKKAEEVIAAHRPTVDAVINKYKARLEGKRVMLYVGGLRPRHVMTAYEDLGMQICGAGYEFAHSDDYQRTTEYAKEGTL
IYDDLTGYELERFIEKLRPDLVGSGIKEKYAVQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINNPVWALLKAPW
TKAAAE

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54471; Mature: 54340

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEYTNDAELSQKAIEEVLEAYPEKAAKKRKKHLGTIVAEGEGSSCGVKSNVKAIPGVM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCE
TIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYFTGQVGVDSFVTMQFTSD
EEEHHEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHHCCCEECCCCCCCEEEEEECCC
FQEKDIVFGGDKKLEKVIDEIKGLFPLVRGISIQSECPIGLIGDDIEAVARKKAKDVGLP
CCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCC
IIPVRCEGFRGVSQSLGHHIANDAIRDWVFSRDSESAFETTPYDVNIIGDYNIGGDAWAS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
RILLEEMGLRVIAQWSGDATIAEMERAPKAKLNLIHCYRSMNYICRHMEEKHGVPWMEYN
HHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
FFGPSQIEKSLRAIAANFDETIQKKAEEVIAAHRPTVDAVINKYKARLEGKRVMLYVGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCC
RPRHVMTAYEDLGMQICGAGYEFAHSDDYQRTTEYAKEGTLIYDDLTGYELERFIEKLRP
CCHHHHHHHHHCCHHEECCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCH
DLVGSGIKEKYAVQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINNPVWALLKAP
HHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEEEECCCHHHCCCHHEEHCCC
WTKAAAE
CHHCCCC
>Mature Secondary Structure 
SLEYTNDAELSQKAIEEVLEAYPEKAAKKRKKHLGTIVAEGEGSSCGVKSNVKAIPGVM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCE
TIRGCAYAGSKGVVWGPVKDMVHISHGPVGCGQYSWSQRRNYFTGQVGVDSFVTMQFTSD
EEEHHEECCCCCCEECCHHHHEEECCCCCCCCCCCHHHHCCCEECCCCCCCEEEEEECCC
FQEKDIVFGGDKKLEKVIDEIKGLFPLVRGISIQSECPIGLIGDDIEAVARKKAKDVGLP
CCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCC
IIPVRCEGFRGVSQSLGHHIANDAIRDWVFSRDSESAFETTPYDVNIIGDYNIGGDAWAS
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHH
RILLEEMGLRVIAQWSGDATIAEMERAPKAKLNLIHCYRSMNYICRHMEEKHGVPWMEYN
HHHHHHCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
FFGPSQIEKSLRAIAANFDETIQKKAEEVIAAHRPTVDAVINKYKARLEGKRVMLYVGGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCC
RPRHVMTAYEDLGMQICGAGYEFAHSDDYQRTTEYAKEGTLIYDDLTGYELERFIEKLRP
CCHHHHHHHHHCCHHEECCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCH
DLVGSGIKEKYAVQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINNPVWALLKAP
HHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCEEEEEECCCHHHCCCHHEEHCCC
WTKAAAE
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]