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The map label for this gene is nifH

Identifier: 83592346

GI number: 83592346

Start: 1196999

End: 1197886

Strand: Direct

Name: nifH

Synonym: Rru_A1010

Alternate gene names: 83592346

Gene position: 1196999-1197886 (Clockwise)

Preceding gene: 83592342

Following gene: 83592347

Centisome position: 27.5

GC content: 61.94

Gene sequence:

>888_bases
ATGAGCGCACTGCGTCAGATCGCGTTCTACGGCAAGGGCGGTATCGGCAAGTCGACGACGTCCCAGAACACGCTGGCCGC
TCTGGTCGAAATGGGCCAGCGGATCCTGATTGTCGGCTGCGATCCCAAGGCCGATTCCACCCGCCTGATCCTGAACACCA
AGCTGCAGGACACCGTTCTTCATCTGGCCGCCGAGGCCGGCTCGGTCGAAGACCTCGACGTCGCCGATGTGGTGAAGATC
GGCTACAAGGGCATCAAGTGCACCGAATCCGGTGGTCCGGAGCCCGGCGTCGGCTGTGCCGGCCGTGGCGTGATCACCGC
CATCAACTTCCTTGAGGAAAACGGCGCCTACGACGACCTCGATTACGTCTCTTACGACGTGCTGGGCGACGTGGTTTGCG
GTGGCTTCGCCATGCCGATCCGCGAGAACAAGGCTCAGGAAATCTACATCGTCATGTCGGGCGAGATGATGGCGCTGTAT
GCCGCCAACAACATCGCCAAGGGCATTTTGAAATACGCCCACACCGGTGGGGTTCGTCTGGGCGGTCTGATCTGCAACGA
GCGTCAGACTGATAAGGAAGTCGAACTGGCCGAGGCCCTGGCCGGCCGTTTGGGCTGCCGCCTCATCCACTTCGTGCCGC
GCGACAACGGCGTTCAGCACGCCGAGCTGCGCCGCCAAACGGTGATCCAATACGCCCCCGACAGCAAGCAGGCCGGTGAG
TATCGCACGCTGGCGACCAAGATCCACAACAACTCCGGCCAGGGCGTGGTGCCGACCCCGATCACCATGGAAGACCTGGA
AGAGATGCTGATGGAATTCGGCATCATGAAGTCCGACGAAGAGGCGCTGGCCGAGCTCGAAGCCAAGGAATCCGCCGCCG
CCAATTAA

Upstream 100 bases:

>100_bases
GGGCAAATCAGGTTTGGCACGCTGGGTGCATTAACGGGGTGAGCGTGGCGGTGGAGCTTCCCCGCCTTAGGACCGGATCA
CGAAGAAAAGGATTGATTCC

Downstream 100 bases:

>100_bases
GGCCGTCGCCGTGCGACCGTTCCCCCCGGCCGGCCGGTCAGGCCGCCAGGGGCGACGGAAGCGGGCGGACCTCCAAGAAG
GCCTCTTCGCCGAGGCGATA

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MSALRQIAFYGKGGIGKSTTSQNTLAALVEMGQRILIVGCDPKADSTRLILNTKLQDTVLHLAAEAGSVEDLDVADVVKI
GYKGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDLDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHTGGVRLGGLICNERQTDKEVELAEALAGRLGCRLIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGE
YRTLATKIHNNSGQGVVPTPITMEDLEEMLMEFGIMKSDEEALAELEAKESAAAN

Sequences:

>Translated_295_residues
MSALRQIAFYGKGGIGKSTTSQNTLAALVEMGQRILIVGCDPKADSTRLILNTKLQDTVLHLAAEAGSVEDLDVADVVKI
GYKGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDLDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALY
AANNIAKGILKYAHTGGVRLGGLICNERQTDKEVELAEALAGRLGCRLIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGE
YRTLATKIHNNSGQGVVPTPITMEDLEEMLMEFGIMKSDEEALAELEAKESAAAN
>Mature_294_residues
SALRQIAFYGKGGIGKSTTSQNTLAALVEMGQRILIVGCDPKADSTRLILNTKLQDTVLHLAAEAGSVEDLDVADVVKIG
YKGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDLDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYA
ANNIAKGILKYAHTGGVRLGGLICNERQTDKEVELAEALAGRLGCRLIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGEY
RTLATKIHNNSGQGVVPTPITMEDLEEMLMEFGIMKSDEEALAELEAKESAAAN

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFH_RHORU (P22921)

Other databases:

- EMBL:   M33774
- PIR:   JW0039
- ProteinModelPortal:   P22921
- SMR:   P22921
- BRENDA:   1.18.6.1
- HAMAP:   MF_00533
- InterPro:   IPR000392
- InterPro:   IPR005977
- PIRSF:   PIRSF000363
- PRINTS:   PR00091
- TIGRFAMs:   TIGR01287

Pfam domain/function: PF00142 Fer4_NifH

EC number: =1.18.6.1

Molecular weight: Translated: 31643; Mature: 31511

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00746 NIFH_FRXC_1; PS00692 NIFH_FRXC_2; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALRQIAFYGKGGIGKSTTSQNTLAALVEMGQRILIVGCDPKADSTRLILNTKLQDTVL
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCHHHHHH
HLAAEAGSVEDLDVADVVKIGYKGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDL
HHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHTGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHCCCCEE
GGLICNERQTDKEVELAEALAGRLGCRLIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGE
CCEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHEEECCCCCCCCC
YRTLATKIHNNSGQGVVPTPITMEDLEEMLMEFGIMKSDEEALAELEAKESAAAN
HHHHHHEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SALRQIAFYGKGGIGKSTTSQNTLAALVEMGQRILIVGCDPKADSTRLILNTKLQDTVL
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCHHHHHH
HLAAEAGSVEDLDVADVVKIGYKGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDL
HHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCH
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALYAANNIAKGILKYAHTGGVRL
HHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEEHHHHHHHHHHHHHCCCCEE
GGLICNERQTDKEVELAEALAGRLGCRLIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGE
CCEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHEEECCCCCCCCC
YRTLATKIHNNSGQGVVPTPITMEDLEEMLMEFGIMKSDEEALAELEAKESAAAN
HHHHHHEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1979299