Click here to switch to the map view.

The map label for this gene is nifB [H]

Identifier: 83592330

GI number: 83592330

Start: 1182865

End: 1184376

Strand: Direct

Name: nifB [H]

Synonym: Rru_A0994

Alternate gene names: 83592330

Gene position: 1182865-1184376 (Clockwise)

Preceding gene: 83592329

Following gene: 83592331

Centisome position: 27.17

GC content: 65.74

Gene sequence:

>1512_bases
GTGTCGGAACAGGTTATATCCCTGGACAGCATATCGCGCCTTGGCGTTCTATCGGCCGCCTCCCGCGACCAAGTGCCGCC
CCCGGCCGCCGCCGAGGGCTGCGCTTCGCAGGGGCGCTGCGGCGCCTCGGCCGGGCCCGATGACATGCCCGCCGAAGTCT
GGGAAAAGGTGAAGAACCATCCCTGCTATTCGGAAGAGGCCCACCATTTCTTCGCCCGCATGCATGTGGCGGTGGCGCCG
GCCTGTAACATCCAGTGCAATTATTGCAACCGCAAGTACGATTGCGCCAATGAAAGCCGCCCCGGAGTCGTCTCCGAACG
GCTGACGCCCGAGCAGGCGGCGCGCAAGGTGGCCGCCGTCGCCAACGAACTGCCCCAGCTTTCGGTGCTTGGCGTCGCCG
GTCCGGGCGACAGCCTGTTTGACGCCCGCAAGACCTTCGAGACCTTCGCCCGGGTCGGCGCGATGCTGCCCGATCTGAAG
TTCTGCATTTCGACCAATGGCTTGGCCCTTCCCGATCATCTTGACGAACTGACCCGCCACGCCATTGATCACGTCACCAT
CACCCTGAACTGCGTCGATCCCGAAATCGGCGCGCGGATCTATCCCTGGATCTATTTCAAGGGCCGGCGCTGGACCGGCG
TCGATGGCGCGAAGATCCTGCTCGAGCGCCAGATGGAAGGGCTTGAAGGGCTGATCGCCCGCAAGATCCTGGTCAAGATC
AACTCGGTGATGATCCCGGGCATCAACGACCACCATCTGCCCGAGGTCAACCGGGTGATCCGCGAGAAGGGGGCGTTCTT
GCACAACGTCATGCCGCTGATCTCGGCGCCCGAGCACGGCACCCACTTCGGGCTGACCGGCCAGCGCGGGCCGACGCCAG
CGGAACTCAAGGACCTGCAGGATCGGCTTGGCGGCGGCGCCAATCTGATGAAGCATTGTCGCCAGTGCCGGGCCGATGCC
ATCGGCCTGCTGGGCGAGGATCGCGGGCAGGAGTTCACCATGGACAAAATTCCCGAAACGGTCGTAGTCGACCCCTCGCG
GCGCGAGGCTTACCGGGCTTTCGTTGAAGAGGAGCGCGCCGATCGTCGGGCCGCCGCCGTCCAGACCGAATCGGCTCTGG
CGCGTGACGCCGCCGTGGGCACGCCCGCCCGACTGGTGGCGATCACCACCAAGGGTGGCGGGCGGATCAACGCCCATTTC
GGTAAGGTCACCGAGTTCCAGATCTACGAGGTGGATGGGGCCGGGGTGCGCTTCGTCGGCGCCCGGCGTTTGCCCGATAA
ATACTGCCTGGGCGGCTTCGATAATGCCCCGGTGATGCCGGCGATCCTTGACGCCCTGGAGGGGGTCGACGCGGTGTTGA
CGGCCAAGATCGGTCCGGGACCGCGCGGGCGCCTGGAAGCGGCCGGCATCGAGGTGTACGAGGACCGCACGCTGGAGTAT
ATCGAGCCCGCCATCGGAAGTTGGTACGCGGGCCGGATGGGAGCGGCGCTCTCTGAACGGTCTTCGGCCTGA

Upstream 100 bases:

>100_bases
CGGTAGGTCTTTCATATCAATGCCTTAGAGCTTTGGCATCATCCTTGCTTTGTTTCCTGGGCAATGCCCATTCCGGGCGA
ACGGACCAAGGAGACGGACG

Downstream 100 bases:

>100_bases
CCGAAGGCGAAGAGACCCGGGTTGCCCCTAAGCGGGCTCCGGGGGGACAAAGACACGGCTTAAGGAGTGACTATCATGGC
CCTGAAGATCGATACCAATT

Product: nitrogenase cofactor biosynthesis protein NifB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSEQVISLDSISRLGVLSAASRDQVPPPAAAEGCASQGRCGASAGPDDMPAEVWEKVKNHPCYSEEAHHFFARMHVAVAP
ACNIQCNYCNRKYDCANESRPGVVSERLTPEQAARKVAAVANELPQLSVLGVAGPGDSLFDARKTFETFARVGAMLPDLK
FCISTNGLALPDHLDELTRHAIDHVTITLNCVDPEIGARIYPWIYFKGRRWTGVDGAKILLERQMEGLEGLIARKILVKI
NSVMIPGINDHHLPEVNRVIREKGAFLHNVMPLISAPEHGTHFGLTGQRGPTPAELKDLQDRLGGGANLMKHCRQCRADA
IGLLGEDRGQEFTMDKIPETVVVDPSRREAYRAFVEEERADRRAAAVQTESALARDAAVGTPARLVAITTKGGGRINAHF
GKVTEFQIYEVDGAGVRFVGARRLPDKYCLGGFDNAPVMPAILDALEGVDAVLTAKIGPGPRGRLEAAGIEVYEDRTLEY
IEPAIGSWYAGRMGAALSERSSA

Sequences:

>Translated_503_residues
MSEQVISLDSISRLGVLSAASRDQVPPPAAAEGCASQGRCGASAGPDDMPAEVWEKVKNHPCYSEEAHHFFARMHVAVAP
ACNIQCNYCNRKYDCANESRPGVVSERLTPEQAARKVAAVANELPQLSVLGVAGPGDSLFDARKTFETFARVGAMLPDLK
FCISTNGLALPDHLDELTRHAIDHVTITLNCVDPEIGARIYPWIYFKGRRWTGVDGAKILLERQMEGLEGLIARKILVKI
NSVMIPGINDHHLPEVNRVIREKGAFLHNVMPLISAPEHGTHFGLTGQRGPTPAELKDLQDRLGGGANLMKHCRQCRADA
IGLLGEDRGQEFTMDKIPETVVVDPSRREAYRAFVEEERADRRAAAVQTESALARDAAVGTPARLVAITTKGGGRINAHF
GKVTEFQIYEVDGAGVRFVGARRLPDKYCLGGFDNAPVMPAILDALEGVDAVLTAKIGPGPRGRLEAAGIEVYEDRTLEY
IEPAIGSWYAGRMGAALSERSSA
>Mature_502_residues
SEQVISLDSISRLGVLSAASRDQVPPPAAAEGCASQGRCGASAGPDDMPAEVWEKVKNHPCYSEEAHHFFARMHVAVAPA
CNIQCNYCNRKYDCANESRPGVVSERLTPEQAARKVAAVANELPQLSVLGVAGPGDSLFDARKTFETFARVGAMLPDLKF
CISTNGLALPDHLDELTRHAIDHVTITLNCVDPEIGARIYPWIYFKGRRWTGVDGAKILLERQMEGLEGLIARKILVKIN
SVMIPGINDHHLPEVNRVIREKGAFLHNVMPLISAPEHGTHFGLTGQRGPTPAELKDLQDRLGGGANLMKHCRQCRADAI
GLLGEDRGQEFTMDKIPETVVVDPSRREAYRAFVEEERADRRAAAVQTESALARDAAVGTPARLVAITTKGGGRINAHFG
KVTEFQIYEVDGAGVRFVGARRLPDKYCLGGFDNAPVMPAILDALEGVDAVLTAKIGPGPRGRLEAAGIEVYEDRTLEYI
EPAIGSWYAGRMGAALSERSSA

Specific function: Probably involved in the synthesis of the Fe-Mo cofactor [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaA/nifB/pqqE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003731
- InterPro:   IPR000385
- InterPro:   IPR005980
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02579 Nitro_FeMo-Co; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 54488; Mature: 54356

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQVISLDSISRLGVLSAASRDQVPPPAAAEGCASQGRCGASAGPDDMPAEVWEKVKNH
CCCCEECHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC
PCYSEEAHHFFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAARKVAAV
CCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHH
ANELPQLSVLGVAGPGDSLFDARKTFETFARVGAMLPDLKFCISTNGLALPDHLDELTRH
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
AIDHVTITLNCVDPEIGARIYPWIYFKGRRWTGVDGAKILLERQMEGLEGLIARKILVKI
HHCEEEEEEEECCCCCCCEEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NSVMIPGINDHHLPEVNRVIREKGAFLHNVMPLISAPEHGTHFGLTGQRGPTPAELKDLQ
HCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHH
DRLGGGANLMKHCRQCRADAIGLLGEDRGQEFTMDKIPETVVVDPSRREAYRAFVEEERA
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHH
DRRAAAVQTESALARDAAVGTPARLVAITTKGGGRINAHFGKVTEFQIYEVDGAGVRFVG
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCEEEEEEEEECCCCEEEEE
ARRLPDKYCLGGFDNAPVMPAILDALEGVDAVLTAKIGPGPRGRLEAAGIEVYEDRTLEY
CCCCCHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCEEEECCCCHHH
IEPAIGSWYAGRMGAALSERSSA
HHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEQVISLDSISRLGVLSAASRDQVPPPAAAEGCASQGRCGASAGPDDMPAEVWEKVKNH
CCCEECHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC
PCYSEEAHHFFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAARKVAAV
CCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHH
ANELPQLSVLGVAGPGDSLFDARKTFETFARVGAMLPDLKFCISTNGLALPDHLDELTRH
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHH
AIDHVTITLNCVDPEIGARIYPWIYFKGRRWTGVDGAKILLERQMEGLEGLIARKILVKI
HHCEEEEEEEECCCCCCCEEEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
NSVMIPGINDHHLPEVNRVIREKGAFLHNVMPLISAPEHGTHFGLTGQRGPTPAELKDLQ
HCEEECCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHH
DRLGGGANLMKHCRQCRADAIGLLGEDRGQEFTMDKIPETVVVDPSRREAYRAFVEEERA
HHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCEEEECCCHHHHHHHHHHHHHH
DRRAAAVQTESALARDAAVGTPARLVAITTKGGGRINAHFGKVTEFQIYEVDGAGVRFVG
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCEEEEEEEEECCCCEEEEE
ARRLPDKYCLGGFDNAPVMPAILDALEGVDAVLTAKIGPGPRGRLEAAGIEVYEDRTLEY
CCCCCHHHHCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCEEEECCCCHHH
IEPAIGSWYAGRMGAALSERSSA
HHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1840608 [H]