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The map label for this gene is nifA [H]

Identifier: 83592329

GI number: 83592329

Start: 1180876

End: 1182678

Strand: Direct

Name: nifA [H]

Synonym: Rru_A0993

Alternate gene names: 83592329

Gene position: 1180876-1182678 (Clockwise)

Preceding gene: 83592328

Following gene: 83592330

Centisome position: 27.13

GC content: 66.44

Gene sequence:

>1803_bases
ATGCCCGATACCGCCGTCGCCGGTCCCCTGTTCCGCCTGCCTCCGTCGCCGGCCGAGGACAGCTCGCTGCCTTTGCTGAC
CCTGTATGAGGTCAGCAAGATCCTGGGGTCGACCCTGGATCTCGAGCATTCGCTCCACGACGTTCTCAATGTCCTCGCCT
CGTATATGCAGATGCGCCGGGGGGTGGTGACGCTGAAGGACGCGCAAAGGCAGCTCGAGGTGGTGGCGGTGTCGGGGATG
ACGCTGCGCAACGCCGTGGAGGGCGAGGCGCGTTATCCCTTGGCCGTCGCCCAGGAGGTCGTAAGCACGGCGATGCCGAT
GATCGTGCCGTCGATGGCCGCCGATCCCCGCTTCGCCGCTTTCGCCGCCGAGGCCGACGGTCTGGACGACGAGGTGCAGT
CGATGATCTGCGTGCCGATCAAGGGCGGGGTGAAGCCGTTTGGCAGCCTGTCGATCGAGCGCCATCGCGACCGGGGAACC
CGCTTCAAATTCGAGCATGATGTGCGCTTCCTGACCATGGTCGCCACCTTGATCGCCCAGACAGTCAGTCTGGAGCGCCG
GGTGGTTGGCGATCGCGACAGGCTGATCGAGGAAAAGGCCCGGCTGGAAAAGGCCCTGCCGAAGCGCGAAAAGCCGTTGA
AGGGGACGATCTTGGAAAACGTCGTCGGCCGGTCGTCGGCGATGATGAAGATTTCCGCCCAGGTTCGCCAGATCGCCCCG
TCGCGGGCGACGGTTCTTTTGCGCGGGGAAAGCGGCACCGGCAAGGAACTGATCGCCCAGGCCATCCACTATCTCAGCCC
GCGCTCCGACAAGCCCTTCATCAAGGTCAATTGCGCCGCCCTGCCCGAGACCTTGCTGGAATCCGAGCTGTTCGGCCACG
AGAAGGGCGCCTTCACCGGGGCGACGACCGAACGCAAGGGCCGCTTCGAAATGGCCAGCGGCGGCACGCTGTTTCTTGAC
GAGATCGGCGAGATCACCGTCCACTTCCAGGCCAAGCTGCTGCGCGTGCTTCAGGAAGGCGAATTCGAGCGGGTTGGCGG
CGGCAAGACGATCAAGGTCGACGTGCGGCTGGTGGCGGCGACCAACAAGGATCTGGAAGACGCGGTGACCCGGGGCGAAT
TCCGCGCCGATCTCTATTACCGCATCAATGTGGTGCCGATCTTCCTGCCGGCCCTGCGCGAGCGCCGCGAGGACGTGCCG
CTGCTCGCCCAGTTCTTTCTGACCAAGTTCAACGAGGAAAACGGCCGCTCGCTGCGCTTCTCGGAAGGGGCCTTGACGGC
GATGGGCGGTTGTAACTTCCCCGGCAATGTCCGCGAGCTGGAGAATTGCGTCTGTCGCGCCGCCACCCTGGCCCAGGACG
AGGTCATCCAGGAACTGGGGCTGTCGTGCCATAACGACAAATGCCTGTCGGCCAGCCTGTGGCAGCGCCGGGGAAGCGGA
CGGGCGATCGGCGGTCTGGCCCCGGTTACCGAGCAGAACGATCCCGATACCACCTCGGTCGCCTGGGAGGGCGATCTTCG
CCCGGCCGCCCCGGCGCGCGCCGGAACGCCACCGGGGCGCGGCTATGCCGGTCCGGGGGAAAGCGCCGATAGCCCGTCTT
CCCCATCGGCCCCGCCGCCCGCCGCCGCGCCGGCCGCCGAGGATGGCGAGGACGAGAGCGACGCCGGCCAGCGCGAGCGC
ATCGTCCACGCCATGGAAAAGGCCGGCTGGGTCCAGGCCAAGGCCGCCCGCTTGTTGGGCATGACGCCGCGACAGATCGG
CTATGCCCTGAAGAAGCACGATATCCCGCTCAAGCGCATGTAG

Upstream 100 bases:

>100_bases
CGGTGGGGTTTGGTTCGCCCGCAAGGGACGTGAAGGTCCGCCACCGCATTGTCGGGACTCTCTTGCCCGGAGTCCCCGGG
TCCTTGAAGGACGTCGTGCC

Downstream 100 bases:

>100_bases
GGCGGAAACGACGGATCTGTCGATATCCCGACCGCCGCCTGTTGCCATTCTTACATTGTCGTAGACTCTACAGGGGGTTT
CAGAGGCGGTAGGTCTTTCA

Product: transcriptional regulator NifA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MPDTAVAGPLFRLPPSPAEDSSLPLLTLYEVSKILGSTLDLEHSLHDVLNVLASYMQMRRGVVTLKDAQRQLEVVAVSGM
TLRNAVEGEARYPLAVAQEVVSTAMPMIVPSMAADPRFAAFAAEADGLDDEVQSMICVPIKGGVKPFGSLSIERHRDRGT
RFKFEHDVRFLTMVATLIAQTVSLERRVVGDRDRLIEEKARLEKALPKREKPLKGTILENVVGRSSAMMKISAQVRQIAP
SRATVLLRGESGTGKELIAQAIHYLSPRSDKPFIKVNCAALPETLLESELFGHEKGAFTGATTERKGRFEMASGGTLFLD
EIGEITVHFQAKLLRVLQEGEFERVGGGKTIKVDVRLVAATNKDLEDAVTRGEFRADLYYRINVVPIFLPALRERREDVP
LLAQFFLTKFNEENGRSLRFSEGALTAMGGCNFPGNVRELENCVCRAATLAQDEVIQELGLSCHNDKCLSASLWQRRGSG
RAIGGLAPVTEQNDPDTTSVAWEGDLRPAAPARAGTPPGRGYAGPGESADSPSSPSAPPPAAAPAAEDGEDESDAGQRER
IVHAMEKAGWVQAKAARLLGMTPRQIGYALKKHDIPLKRM

Sequences:

>Translated_600_residues
MPDTAVAGPLFRLPPSPAEDSSLPLLTLYEVSKILGSTLDLEHSLHDVLNVLASYMQMRRGVVTLKDAQRQLEVVAVSGM
TLRNAVEGEARYPLAVAQEVVSTAMPMIVPSMAADPRFAAFAAEADGLDDEVQSMICVPIKGGVKPFGSLSIERHRDRGT
RFKFEHDVRFLTMVATLIAQTVSLERRVVGDRDRLIEEKARLEKALPKREKPLKGTILENVVGRSSAMMKISAQVRQIAP
SRATVLLRGESGTGKELIAQAIHYLSPRSDKPFIKVNCAALPETLLESELFGHEKGAFTGATTERKGRFEMASGGTLFLD
EIGEITVHFQAKLLRVLQEGEFERVGGGKTIKVDVRLVAATNKDLEDAVTRGEFRADLYYRINVVPIFLPALRERREDVP
LLAQFFLTKFNEENGRSLRFSEGALTAMGGCNFPGNVRELENCVCRAATLAQDEVIQELGLSCHNDKCLSASLWQRRGSG
RAIGGLAPVTEQNDPDTTSVAWEGDLRPAAPARAGTPPGRGYAGPGESADSPSSPSAPPPAAAPAAEDGEDESDAGQRER
IVHAMEKAGWVQAKAARLLGMTPRQIGYALKKHDIPLKRM
>Mature_599_residues
PDTAVAGPLFRLPPSPAEDSSLPLLTLYEVSKILGSTLDLEHSLHDVLNVLASYMQMRRGVVTLKDAQRQLEVVAVSGMT
LRNAVEGEARYPLAVAQEVVSTAMPMIVPSMAADPRFAAFAAEADGLDDEVQSMICVPIKGGVKPFGSLSIERHRDRGTR
FKFEHDVRFLTMVATLIAQTVSLERRVVGDRDRLIEEKARLEKALPKREKPLKGTILENVVGRSSAMMKISAQVRQIAPS
RATVLLRGESGTGKELIAQAIHYLSPRSDKPFIKVNCAALPETLLESELFGHEKGAFTGATTERKGRFEMASGGTLFLDE
IGEITVHFQAKLLRVLQEGEFERVGGGKTIKVDVRLVAATNKDLEDAVTRGEFRADLYYRINVVPIFLPALRERREDVPL
LAQFFLTKFNEENGRSLRFSEGALTAMGGCNFPGNVRELENCVCRAATLAQDEVIQELGLSCHNDKCLSASLWQRRGSGR
AIGGLAPVTEQNDPDTTSVAWEGDLRPAAPARAGTPPGRGYAGPGESADSPSSPSAPPPAAAPAAEDGEDESDAGQRERI
VHAMEKAGWVQAKAARLLGMTPRQIGYALKKHDIPLKRM

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=238, Percent_Identity=57.9831932773109, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1790437, Length=266, Percent_Identity=53.7593984962406, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI1789087, Length=336, Percent_Identity=43.452380952381, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87082117, Length=257, Percent_Identity=52.1400778210117, Blast_Score=265, Evalue=8e-72,
Organism=Escherichia coli, GI87082152, Length=267, Percent_Identity=49.063670411985, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI1788905, Length=241, Percent_Identity=51.0373443983402, Blast_Score=247, Evalue=2e-66,
Organism=Escherichia coli, GI1790299, Length=247, Percent_Identity=51.417004048583, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=49.5689655172414, Blast_Score=217, Evalue=2e-57,
Organism=Escherichia coli, GI1789233, Length=229, Percent_Identity=47.1615720524017, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1786524, Length=249, Percent_Identity=44.578313253012, Blast_Score=192, Evalue=7e-50,
Organism=Escherichia coli, GI1787583, Length=233, Percent_Identity=42.4892703862661, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1789828, Length=241, Percent_Identity=38.1742738589212, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081858, Length=232, Percent_Identity=35.3448275862069, Blast_Score=132, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 65234; Mature: 65103

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTAVAGPLFRLPPSPAEDSSLPLLTLYEVSKILGSTLDLEHSLHDVLNVLASYMQMRR
CCCCCCCCCEEECCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GVVTLKDAQRQLEVVAVSGMTLRNAVEGEARYPLAVAQEVVSTAMPMIVPSMAADPRFAA
CCEEEHHHHHCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
FAAEADGLDDEVQSMICVPIKGGVKPFGSLSIERHRDRGTRFKFEHDVRFLTMVATLIAQ
EECCCCCCCHHHHCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHH
TVSLERRVVGDRDRLIEEKARLEKALPKREKPLKGTILENVVGRSSAMMKISAQVRQIAP
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
SRATVLLRGESGTGKELIAQAIHYLSPRSDKPFIKVNCAALPETLLESELFGHEKGAFTG
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCC
ATTERKGRFEMASGGTLFLDEIGEITVHFQAKLLRVLQEGEFERVGGGKTIKVDVRLVAA
CCCCCCCCEEECCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEECCCCCEEEEEEEEEEE
TNKDLEDAVTRGEFRADLYYRINVVPIFLPALRERREDVPLLAQFFLTKFNEENGRSLRF
CCCCHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEE
SEGALTAMGGCNFPGNVRELENCVCRAATLAQDEVIQELGLSCHNDKCLSASLWQRRGSG
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
RAIGGLAPVTEQNDPDTTSVAWEGDLRPAAPARAGTPPGRGYAGPGESADSPSSPSAPPP
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAAPAAEDGEDESDAGQRERIVHAMEKAGWVQAKAARLLGMTPRQIGYALKKHDIPLKRM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PDTAVAGPLFRLPPSPAEDSSLPLLTLYEVSKILGSTLDLEHSLHDVLNVLASYMQMRR
CCCCCCCCEEECCCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GVVTLKDAQRQLEVVAVSGMTLRNAVEGEARYPLAVAQEVVSTAMPMIVPSMAADPRFAA
CCEEEHHHHHCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEE
FAAEADGLDDEVQSMICVPIKGGVKPFGSLSIERHRDRGTRFKFEHDVRFLTMVATLIAQ
EECCCCCCCHHHHCEEEEECCCCCCCCCCCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHH
TVSLERRVVGDRDRLIEEKARLEKALPKREKPLKGTILENVVGRSSAMMKISAQVRQIAP
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
SRATVLLRGESGTGKELIAQAIHYLSPRSDKPFIKVNCAALPETLLESELFGHEKGAFTG
CCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCC
ATTERKGRFEMASGGTLFLDEIGEITVHFQAKLLRVLQEGEFERVGGGKTIKVDVRLVAA
CCCCCCCCEEECCCCEEEEECCCCEEEHHHHHHHHHHHCCCCEECCCCCEEEEEEEEEEE
TNKDLEDAVTRGEFRADLYYRINVVPIFLPALRERREDVPLLAQFFLTKFNEENGRSLRF
CCCCHHHHHHHCCCEEEEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEE
SEGALTAMGGCNFPGNVRELENCVCRAATLAQDEVIQELGLSCHNDKCLSASLWQRRGSG
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCC
RAIGGLAPVTEQNDPDTTSVAWEGDLRPAAPARAGTPPGRGYAGPGESADSPSSPSAPPP
CCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAAPAAEDGEDESDAGQRERIVHAMEKAGWVQAKAARLLGMTPRQIGYALKKHDIPLKRM
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1779763; 1362170 [H]