Click here to switch to the map view.

The map label for this gene is murF [H]

Identifier: 83592290

GI number: 83592290

Start: 1138485

End: 1139951

Strand: Reverse

Name: murF [H]

Synonym: Rru_A0954

Alternate gene names: 83592290

Gene position: 1139951-1138485 (Counterclockwise)

Preceding gene: 83592291

Following gene: 83592289

Centisome position: 26.19

GC content: 70.21

Gene sequence:

>1467_bases
ATGAAAACCTCGCCCCTGCCAGCCGAGCGCGCCACTCCCCTTGTGTTGTGGACCGCCGCCACCGCCGCCGCCGCGCTTGG
CATTGCCGCCAGCGGCGCGTGGACGGCGACCGGCGTCTGCATCGACAGCCGCGCCGTCGTCGCCGGCGATCTATTCATCG
CCCTGGCCGGCGAACGAACCGATGGCCATGCCCATGTCGAGGCCGCCCTGACTGCCGGAGCCAGCGCCGCCCTGGTCTCG
CGCATCCCCGCCGGCATCGCCACCGACCGCCTGCTGGTGGTCGAAGATACCCTCGAGGGTCTGTGGGCCCTGGCCCGGGC
CAGCCGCGCCCGCTTCGCCGGCAAGCTGGTCGGCGTGACCGGCAGCCTGGGCAAGACCGGCACCAAGGAGATGCTGACCC
AGGCGCTTGGCCGCTTCGGCCGCGTCCATGCGACCGAGGGCAATCTCAACAACCATTTCGGCCTGCCGCTGACCCTGGCC
CGCATGCCCGCCGACACCGATCTGGCGGTGATCGAACTGGGGATGAACCATCCGGGCGAATTGGGGCCGCTGGCCCGCCT
CGCCCGCCCCCATGTGGCGATCATCACCAATGTCGCCGCCGTCCACCTGGAGTTCTTCGATTCGGTCGAGGCCATCGCCG
ATGCCAAGGCCGAGATCCTGGAAGGCGTCGAGCCCGGCGGCACCGTCGTCCTCAATCGCGACAACCGCTTCTTCGAACGC
CTGCGCCGGGCCGCCGCCGCCCGGGGCATCGATAAGACCCTCAGCTTTGGCAATCACATCGGCGCCGACGCCCGGGTGCT
CGACTGCGCCGTCGACGCCAATGGCACGGCGGTCTTCGCCCTGATCGGCGACGACCCCCTGGCTTATCGCATGGCCGTTG
AAGGTCGCCATTGGGCGCTCAACAGCCTTGGCGTGCTGGCGGTTGTCCGCGCCCTGGGTCTTGACATCCACAGCGCCGCC
AACGGCTTGGCCAATGTGGTCCCGCCGCGCGGCCGGGGCCGGCGCCAAAGCGTCAGCCTGGGTGGGGATGGCGCCATCCA
GGTGATCGACGACGCCTATAACGCCAGTCCCGAGAGCGTCATCGCCGCCCTCACCACTTTGTCGATGACCCGCCCGGGGC
CCCGCGGCCGGCGCATCGCCGTGCTTGGCGACATGCTCGAGCTGGGTCCCCAGGCCGCGAAGCTTCACGCCGATCTGGCG
AAGATCAGCGTGGAACGGGGCATTGATCTGGTTTATACCGCCGGCCCCCTGATGGCGCATCTTCACGACGCGCTGCCCGC
CAGTCGGCGCGGCGGCCATGCGCCCGACGCCGATGCCCTGACACCCCTGGTTCTCGCGGCCCTGCGCCCGGGCGACGTGG
TGATGGTCAAAGGGTCGAAGGGAAGCCTCGTCAGCAGGACCGTCACGGCCCTGCTGGCTCTCGATCGCCGCGCGGAGGCG
GGCCTTTCGGCCGCCGACACGCCCTGA

Upstream 100 bases:

>100_bases
TCGCCGGCAAGGGCCATGAAACCGGCCAGACCATCGGCACCGTGGTTCATCCCTTCGTCGATGCCGATCACGCCCGCGCC
GCCGTTGGGGAGGCCGACGC

Downstream 100 bases:

>100_bases
TTAACCCCCTGCTGGGAAGCGGAACACCAGGGAATGCTGTACTTCCTGTCCGAACTCACCGCGCAAGTGAGCGCGCTCAA
CGTCTTCCGGTACCTGACCT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKTSPLPAERATPLVLWTAATAAAALGIAASGAWTATGVCIDSRAVVAGDLFIALAGERTDGHAHVEAALTAGASAALVS
RIPAGIATDRLLVVEDTLEGLWALARASRARFAGKLVGVTGSLGKTGTKEMLTQALGRFGRVHATEGNLNNHFGLPLTLA
RMPADTDLAVIELGMNHPGELGPLARLARPHVAIITNVAAVHLEFFDSVEAIADAKAEILEGVEPGGTVVLNRDNRFFER
LRRAAAARGIDKTLSFGNHIGADARVLDCAVDANGTAVFALIGDDPLAYRMAVEGRHWALNSLGVLAVVRALGLDIHSAA
NGLANVVPPRGRGRRQSVSLGGDGAIQVIDDAYNASPESVIAALTTLSMTRPGPRGRRIAVLGDMLELGPQAAKLHADLA
KISVERGIDLVYTAGPLMAHLHDALPASRRGGHAPDADALTPLVLAALRPGDVVMVKGSKGSLVSRTVTALLALDRRAEA
GLSAADTP

Sequences:

>Translated_488_residues
MKTSPLPAERATPLVLWTAATAAAALGIAASGAWTATGVCIDSRAVVAGDLFIALAGERTDGHAHVEAALTAGASAALVS
RIPAGIATDRLLVVEDTLEGLWALARASRARFAGKLVGVTGSLGKTGTKEMLTQALGRFGRVHATEGNLNNHFGLPLTLA
RMPADTDLAVIELGMNHPGELGPLARLARPHVAIITNVAAVHLEFFDSVEAIADAKAEILEGVEPGGTVVLNRDNRFFER
LRRAAAARGIDKTLSFGNHIGADARVLDCAVDANGTAVFALIGDDPLAYRMAVEGRHWALNSLGVLAVVRALGLDIHSAA
NGLANVVPPRGRGRRQSVSLGGDGAIQVIDDAYNASPESVIAALTTLSMTRPGPRGRRIAVLGDMLELGPQAAKLHADLA
KISVERGIDLVYTAGPLMAHLHDALPASRRGGHAPDADALTPLVLAALRPGDVVMVKGSKGSLVSRTVTALLALDRRAEA
GLSAADTP
>Mature_488_residues
MKTSPLPAERATPLVLWTAATAAAALGIAASGAWTATGVCIDSRAVVAGDLFIALAGERTDGHAHVEAALTAGASAALVS
RIPAGIATDRLLVVEDTLEGLWALARASRARFAGKLVGVTGSLGKTGTKEMLTQALGRFGRVHATEGNLNNHFGLPLTLA
RMPADTDLAVIELGMNHPGELGPLARLARPHVAIITNVAAVHLEFFDSVEAIADAKAEILEGVEPGGTVVLNRDNRFFER
LRRAAAARGIDKTLSFGNHIGADARVLDCAVDANGTAVFALIGDDPLAYRMAVEGRHWALNSLGVLAVVRALGLDIHSAA
NGLANVVPPRGRGRRQSVSLGGDGAIQVIDDAYNASPESVIAALTTLSMTRPGPRGRRIAVLGDMLELGPQAAKLHADLA
KISVERGIDLVYTAGPLMAHLHDALPASRRGGHAPDADALTPLVLAALRPGDVVMVKGSKGSLVSRTVTALLALDRRAEA
GLSAADTP

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=442, Percent_Identity=34.6153846153846, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50413; Mature: 50413

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSPLPAERATPLVLWTAATAAAALGIAASGAWTATGVCIDSRAVVAGDLFIALAGERT
CCCCCCCHHHCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEECCCEEEECEEEEEEECCCC
DGHAHVEAALTAGASAALVSRIPAGIATDRLLVVEDTLEGLWALARASRARFAGKLVGVT
CCCCEEEHHHHCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEEC
GSLGKTGTKEMLTQALGRFGRVHATEGNLNNHFGLPLTLARMPADTDLAVIELGMNHPGE
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCC
LGPLARLARPHVAIITNVAAVHLEFFDSVEAIADAKAEILEGVEPGGTVVLNRDNRFFER
CCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHH
LRRAAAARGIDKTLSFGNHIGADARVLDCAVDANGTAVFALIGDDPLAYRMAVEGRHWAL
HHHHHHHCCCHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCEEH
NSLGVLAVVRALGLDIHSAANGLANVVPPRGRGRRQSVSLGGDGAIQVIDDAYNASPESV
HHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCCCCCEEECCCCCEEEEEECCCCCCHHHH
IAALTTLSMTRPGPRGRRIAVLGDMLELGPQAAKLHADLAKISVERGIDLVYTAGPLMAH
HHHHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
LHDALPASRRGGHAPDADALTPLVLAALRPGDVVMVKGSKGSLVSRTVTALLALDRRAEA
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GLSAADTP
CCCCCCCC
>Mature Secondary Structure
MKTSPLPAERATPLVLWTAATAAAALGIAASGAWTATGVCIDSRAVVAGDLFIALAGERT
CCCCCCCHHHCCCEEEEEHHHHHHHHHHHCCCCCEEEEEEECCCEEEECEEEEEEECCCC
DGHAHVEAALTAGASAALVSRIPAGIATDRLLVVEDTLEGLWALARASRARFAGKLVGVT
CCCCEEEHHHHCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHEEEEEC
GSLGKTGTKEMLTQALGRFGRVHATEGNLNNHFGLPLTLARMPADTDLAVIELGMNHPGE
CCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCCC
LGPLARLARPHVAIITNVAAVHLEFFDSVEAIADAKAEILEGVEPGGTVVLNRDNRFFER
CCHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHH
LRRAAAARGIDKTLSFGNHIGADARVLDCAVDANGTAVFALIGDDPLAYRMAVEGRHWAL
HHHHHHHCCCHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEECCCCEEEEEEECCCCEEH
NSLGVLAVVRALGLDIHSAANGLANVVPPRGRGRRQSVSLGGDGAIQVIDDAYNASPESV
HHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCCCCCCEEECCCCCEEEEEECCCCCCHHHH
IAALTTLSMTRPGPRGRRIAVLGDMLELGPQAAKLHADLAKISVERGIDLVYTAGPLMAH
HHHHHHHHCCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
LHDALPASRRGGHAPDADALTPLVLAALRPGDVVMVKGSKGSLVSRTVTALLALDRRAEA
HHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
GLSAADTP
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]