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The map label for this gene is murE [H]

Identifier: 83592291

GI number: 83592291

Start: 1139948

End: 1141429

Strand: Reverse

Name: murE [H]

Synonym: Rru_A0955

Alternate gene names: 83592291

Gene position: 1141429-1139948 (Counterclockwise)

Preceding gene: 83592292

Following gene: 83592290

Centisome position: 26.22

GC content: 71.66

Gene sequence:

>1482_bases
GTGTATCTCGATGATCTGATCGGCGCCGTTCCGGCGCGGGCCGCCCTGGGCGGCCACCCTGGCCTGGGCGGCCATCCTGG
TTTGGCGATCGCCGGGCTGACGGCCGATTCCCGGGCGGTGGCGCCGGGCTGGCTGTTCGCCGCCCTGCCCGGGGTCAAGG
CCGACGGCCGCGCCTTCATCCCCGCCGCCCTCGCGGCCGGGGCGAGCGCCGTTCTGGCGCCCGAGGGAACGGCCCTGCCC
CCTGACGCCCCGGCGGCGTTGGTCACCGCGGCCAATCCCCGCCAGGCCTTCGCCCGCATGGCCGCCGCCTTCGCCGGCGC
CCAACCGGCCACGGTGGTGGCGGTGACCGGTACCAACGGCAAGACCTCGAGCGTCAGCTTCACCCGTCAGATCTGGGACC
GCCTGGGGCGGACGGCGGCCAGCCTGGGGACGCTCGGCGCCGAAAGCGGCGTTTATCGGCGGGCCGGCAGCATGACCACC
CCCGATCCGGTGTCTTTGCACAAAATGCTGGCCGAGATGACCGAAGCCGGGGTCGACGCCCTGGCGATGGAGGCCTCGTC
CCATGGCCTGGATCAATACCGCCTTGACGGCGTGCGCCTGAGTGCTGCCGCCTTCACCAATCTGACCCGCGACCATCTCG
ACTATCACCACACCATGGACGCCTATCTGGCGGCCAAGGCCCGGCTGTTTGGCGAGGTTCTGGGGCGCGAGGGTGTGGCC
GTGCTCAATGCCGACGATCCGGCGTTCCCCACCCTGGCCGCGCTCTGCCGCGACCGAGGCCAAAGCGTGCTTGATTACGG
CCGGAGCGCCAAGACCCTGCGCCTTGCCGGCGTCAGCGCCAAGGCCCATGGCCAGAGCCTGGACCTCGAGCTTTTTGGCC
GGCACCATAGCGTGCTGTTGCCCTTGGTCGGCGATTTCCAGGCGATGAACGTGCTCTGCGCCCTGGGACTGGCGATCGCC
ACCGGCGCGCCGGTCGACGCCGCCGTGGCGACCCTGGCCAGCCTGGAAGGCGTGCCGGGCCGCTTGGAGATGGTGGCGCG
GCGGGCCTCGGGCGCCCCGGTCTACGTCGATTACGCCCATACTCCCGACGGTCTGGTCACCGCCTTGCGGGCCCTGCGCC
CCCATTGCCGGGGCAAGCTGGTCTGCGTTTTCGGCTGCGGCGGCGACCGCGACCGCGCCAAACGGCCGCTGATGGGCCGC
GCCGTGGCCGAGGGCGCCGATGTGGCGATCCTGACCGATGACAATCCGCGCAGCGAGGATCCGGCCGCCATCCGCGCCGA
AGCCCGCGTTGGCTGCCCCGACGCGATCGAGATCGACGGCCGCGACGCCGCCATCCGCGCAGGCGTCGCCCTGCTTGGAC
CAAACGACCTGCTGGTGATCGCCGGCAAGGGCCATGAAACCGGCCAGACCATCGGCACCGTGGTTCATCCCTTCGTCGAT
GCCGATCACGCCCGCGCCGCCGTTGGGGAGGCCGACGCATGA

Upstream 100 bases:

>100_bases
CTTGGAGTGCAACCCGTGCGCGGCGATCCCGCCGTTCAGTTCGGTCCCCAGCTTGTCCAGCAGGTGGGCCTGCGTTTTGC
CGAGCCCGAAGGGGGACGCG

Downstream 100 bases:

>100_bases
AAACCTCGCCCCTGCCAGCCGAGCGCGCCACTCCCCTTGTGTTGTGGACCGCCGCCACCGCCGCCGCCGCGCTTGGCATT
GCCGCCAGCGGCGCGTGGAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MYLDDLIGAVPARAALGGHPGLGGHPGLAIAGLTADSRAVAPGWLFAALPGVKADGRAFIPAALAAGASAVLAPEGTALP
PDAPAALVTAANPRQAFARMAAAFAGAQPATVVAVTGTNGKTSSVSFTRQIWDRLGRTAASLGTLGAESGVYRRAGSMTT
PDPVSLHKMLAEMTEAGVDALAMEASSHGLDQYRLDGVRLSAAAFTNLTRDHLDYHHTMDAYLAAKARLFGEVLGREGVA
VLNADDPAFPTLAALCRDRGQSVLDYGRSAKTLRLAGVSAKAHGQSLDLELFGRHHSVLLPLVGDFQAMNVLCALGLAIA
TGAPVDAAVATLASLEGVPGRLEMVARRASGAPVYVDYAHTPDGLVTALRALRPHCRGKLVCVFGCGGDRDRAKRPLMGR
AVAEGADVAILTDDNPRSEDPAAIRAEARVGCPDAIEIDGRDAAIRAGVALLGPNDLLVIAGKGHETGQTIGTVVHPFVD
ADHARAAVGEADA

Sequences:

>Translated_493_residues
MYLDDLIGAVPARAALGGHPGLGGHPGLAIAGLTADSRAVAPGWLFAALPGVKADGRAFIPAALAAGASAVLAPEGTALP
PDAPAALVTAANPRQAFARMAAAFAGAQPATVVAVTGTNGKTSSVSFTRQIWDRLGRTAASLGTLGAESGVYRRAGSMTT
PDPVSLHKMLAEMTEAGVDALAMEASSHGLDQYRLDGVRLSAAAFTNLTRDHLDYHHTMDAYLAAKARLFGEVLGREGVA
VLNADDPAFPTLAALCRDRGQSVLDYGRSAKTLRLAGVSAKAHGQSLDLELFGRHHSVLLPLVGDFQAMNVLCALGLAIA
TGAPVDAAVATLASLEGVPGRLEMVARRASGAPVYVDYAHTPDGLVTALRALRPHCRGKLVCVFGCGGDRDRAKRPLMGR
AVAEGADVAILTDDNPRSEDPAAIRAEARVGCPDAIEIDGRDAAIRAGVALLGPNDLLVIAGKGHETGQTIGTVVHPFVD
ADHARAAVGEADA
>Mature_493_residues
MYLDDLIGAVPARAALGGHPGLGGHPGLAIAGLTADSRAVAPGWLFAALPGVKADGRAFIPAALAAGASAVLAPEGTALP
PDAPAALVTAANPRQAFARMAAAFAGAQPATVVAVTGTNGKTSSVSFTRQIWDRLGRTAASLGTLGAESGVYRRAGSMTT
PDPVSLHKMLAEMTEAGVDALAMEASSHGLDQYRLDGVRLSAAAFTNLTRDHLDYHHTMDAYLAAKARLFGEVLGREGVA
VLNADDPAFPTLAALCRDRGQSVLDYGRSAKTLRLAGVSAKAHGQSLDLELFGRHHSVLLPLVGDFQAMNVLCALGLAIA
TGAPVDAAVATLASLEGVPGRLEMVARRASGAPVYVDYAHTPDGLVTALRALRPHCRGKLVCVFGCGGDRDRAKRPLMGR
AVAEGADVAILTDDNPRSEDPAAIRAEARVGCPDAIEIDGRDAAIRAGVALLGPNDLLVIAGKGHETGQTIGTVVHPFVD
ADHARAAVGEADA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=478, Percent_Identity=38.4937238493724, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1786274, Length=315, Percent_Identity=30.1587301587302, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 50473; Mature: 50473

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLDDLIGAVPARAALGGHPGLGGHPGLAIAGLTADSRAVAPGWLFAALPGVKADGRAFI
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCEEH
PAALAAGASAVLAPEGTALPPDAPAALVTAANPRQAFARMAAAFAGAQPATVVAVTGTNG
HHHHHCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCC
KTSSVSFTRQIWDRLGRTAASLGTLGAESGVYRRAGSMTTPDPVSLHKMLAEMTEAGVDA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCHHH
LAMEASSHGLDQYRLDGVRLSAAAFTNLTRDHLDYHHTMDAYLAAKARLFGEVLGREGVA
EEEEHHHCCCCCEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VLNADDPAFPTLAALCRDRGQSVLDYGRSAKTLRLAGVSAKAHGQSLDLELFGRHHSVLL
EEECCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCEEECCCCEEEE
PLVGDFQAMNVLCALGLAIATGAPVDAAVATLASLEGVPGRLEMVARRASGAPVYVDYAH
EHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECC
TPDGLVTALRALRPHCRGKLVCVFGCGGDRDRAKRPLMGRAVAEGADVAILTDDNPRSED
CCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHCCCCEEEEECCCCCCCC
PAAIRAEARVGCPDAIEIDGRDAAIRAGVALLGPNDLLVIAGKGHETGQTIGTVVHPFVD
CCEEEECCCCCCCCCEEECCCCHHHHCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCC
ADHARAAVGEADA
CCHHHHCCCCCCC
>Mature Secondary Structure
MYLDDLIGAVPARAALGGHPGLGGHPGLAIAGLTADSRAVAPGWLFAALPGVKADGRAFI
CCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCCCCEEH
PAALAAGASAVLAPEGTALPPDAPAALVTAANPRQAFARMAAAFAGAQPATVVAVTGTNG
HHHHHCCCCEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEECCCC
KTSSVSFTRQIWDRLGRTAASLGTLGAESGVYRRAGSMTTPDPVSLHKMLAEMTEAGVDA
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCHHH
LAMEASSHGLDQYRLDGVRLSAAAFTNLTRDHLDYHHTMDAYLAAKARLFGEVLGREGVA
EEEEHHHCCCCCEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VLNADDPAFPTLAALCRDRGQSVLDYGRSAKTLRLAGVSAKAHGQSLDLELFGRHHSVLL
EEECCCCCHHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCCCCCCCEEECCCCEEEE
PLVGDFQAMNVLCALGLAIATGAPVDAAVATLASLEGVPGRLEMVARRASGAPVYVDYAH
EHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECC
TPDGLVTALRALRPHCRGKLVCVFGCGGDRDRAKRPLMGRAVAEGADVAILTDDNPRSED
CCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHCCCCEEEEECCCCCCCC
PAAIRAEARVGCPDAIEIDGRDAAIRAGVALLGPNDLLVIAGKGHETGQTIGTVVHPFVD
CCEEEECCCCCCCCCEEECCCCHHHHCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCC
ADHARAAVGEADA
CCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]