Click here to switch to the map view.

The map label for this gene is ddl

Identifier: 83592283

GI number: 83592283

Start: 1130284

End: 1131192

Strand: Reverse

Name: ddl

Synonym: Rru_A0947

Alternate gene names: 83592283

Gene position: 1131192-1130284 (Counterclockwise)

Preceding gene: 83592284

Following gene: 83592282

Centisome position: 25.99

GC content: 66.89

Gene sequence:

>909_bases
ATGAGTAAGCGCGTGACCGTTCTGATGGGCGGCTTTTCGACCGAGCGTGCGGTGTCGCTAAACAGCGGCGCGGCGGCCGG
CGAGGCCTTGCGTCAGGCCGGCTACGCCGTGACCTTGCTCGATGTCGGCCGCGATGTCGCCGCCTTCATCGCCGCCCTCA
CCCAAACCCGCCCGGATGTGGTGTTCAACGCCCTCCACGGCCGTTTCGGCGAGGACGGCGCCATCCAGGGCATCCTTGAC
ATCATGGCCCTGCCCTATACCCATTCGGGCCGGCTGGCCAGCGCCATCGCCATGGACAAGCCCAGCGCCAAGCTGGTGTT
CGAGCGCTTCGCCATCCCCGTCGCCCCCCATGTCGTCGCCGACCGCGCCAGCGTTCTGGCCGAAACCGCCATGGCCCGCC
CCTATGTGGTCAAGCCCCTTGATCAGGGGTCGAGCGTCGGGGTGACCATCGTCACCAGCGAAACCAATGATCTGCCGTTT
TCGCGCGACGACTGGCCCTATGGCCGTCAGGTGATGGTCGAACGCTTCATCCCCGGCCGCGAACTGACCGTCGGCGTCAT
GGGCGACAAGGCCCTGGGCGTCACCGAGATCGTCACCGACCACCGCTTCTACGACTACGACGCCAAATACGCCCAGGGCG
GTTCGCGCCATATCGTGCCCGCCCCGGTCGCCCCCGAGGTCTTCGCCCAGGCGCAAGAGCTTTCGGTGCTGGCCCATCAG
GCCCTGGGATGCCGGGGCGTCAGCCGCGCCGACCTGCGCTTCGACGGCGAAACCTTGTACATGCTCGAGGTCAATACCCA
GCCGGGGATGACGGACACCTCGTTGGTCCCCGAGCAGGCCCTTCATGCCGGCATCTCCTTTCCCGATCTGGTCACCTGGC
TCGTGGAGACCGCCCAATGCGACGCGTGA

Upstream 100 bases:

>100_bases
CCGTGCGGCGCCGGGTCATGGACACCTCGGGGGTCGCCCTGCGGTGGGAAATCAAGCGAATCGGGATCGGTCTTGACGGC
CTTTCCGCGGGAGAAAACGG

Downstream 100 bases:

>100_bases
GCCAACCACCGCCCCGTCCGCGCCACCGCCGCCGCAGAAGACGCGATGTCCTGCCCTCGCTGCCGCGCGCCAGCATGGTC
TTCCTGATCGCCGGCGGCGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSKRVTVLMGGFSTERAVSLNSGAAAGEALRQAGYAVTLLDVGRDVAAFIAALTQTRPDVVFNALHGRFGEDGAIQGILD
IMALPYTHSGRLASAIAMDKPSAKLVFERFAIPVAPHVVADRASVLAETAMARPYVVKPLDQGSSVGVTIVTSETNDLPF
SRDDWPYGRQVMVERFIPGRELTVGVMGDKALGVTEIVTDHRFYDYDAKYAQGGSRHIVPAPVAPEVFAQAQELSVLAHQ
ALGCRGVSRADLRFDGETLYMLEVNTQPGMTDTSLVPEQALHAGISFPDLVTWLVETAQCDA

Sequences:

>Translated_302_residues
MSKRVTVLMGGFSTERAVSLNSGAAAGEALRQAGYAVTLLDVGRDVAAFIAALTQTRPDVVFNALHGRFGEDGAIQGILD
IMALPYTHSGRLASAIAMDKPSAKLVFERFAIPVAPHVVADRASVLAETAMARPYVVKPLDQGSSVGVTIVTSETNDLPF
SRDDWPYGRQVMVERFIPGRELTVGVMGDKALGVTEIVTDHRFYDYDAKYAQGGSRHIVPAPVAPEVFAQAQELSVLAHQ
ALGCRGVSRADLRFDGETLYMLEVNTQPGMTDTSLVPEQALHAGISFPDLVTWLVETAQCDA
>Mature_301_residues
SKRVTVLMGGFSTERAVSLNSGAAAGEALRQAGYAVTLLDVGRDVAAFIAALTQTRPDVVFNALHGRFGEDGAIQGILDI
MALPYTHSGRLASAIAMDKPSAKLVFERFAIPVAPHVVADRASVLAETAMARPYVVKPLDQGSSVGVTIVTSETNDLPFS
RDDWPYGRQVMVERFIPGRELTVGVMGDKALGVTEIVTDHRFYDYDAKYAQGGSRHIVPAPVAPEVFAQAQELSVLAHQA
LGCRGVSRADLRFDGETLYMLEVNTQPGMTDTSLVPEQALHAGISFPDLVTWLVETAQCDA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=311, Percent_Identity=41.1575562700965, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1786579, Length=351, Percent_Identity=33.048433048433, Blast_Score=164, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_RHORT (Q2RVU7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426035.1
- ProteinModelPortal:   Q2RVU7
- SMR:   Q2RVU7
- STRING:   Q2RVU7
- GeneID:   3834404
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0947
- NMPDR:   fig|1085.1.peg.3425
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   PLSIVPE
- PhylomeDB:   Q2RVU7
- ProtClustDB:   PRK01372
- BioCyc:   RRUB269796:RRU_A0947-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32390; Mature: 32259

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRVTVLMGGFSTERAVSLNSGAAAGEALRQAGYAVTLLDVGRDVAAFIAALTQTRPDV
CCCEEEEEEECCCCCCEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCHHH
VFNALHGRFGEDGAIQGILDIMALPYTHSGRLASAIAMDKPSAKLVFERFAIPVAPHVVA
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
DRASVLAETAMARPYVVKPLDQGSSVGVTIVTSETNDLPFSRDDWPYGRQVMVERFIPGR
HHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCC
ELTVGVMGDKALGVTEIVTDHRFYDYDAKYAQGGSRHIVPAPVAPEVFAQAQELSVLAHQ
EEEEEECCCCCCCHHHHHHCCEEECCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHH
ALGCRGVSRADLRFDGETLYMLEVNTQPGMTDTSLVPEQALHAGISFPDLVTWLVETAQC
HHCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCC
DA
CC
>Mature Secondary Structure 
SKRVTVLMGGFSTERAVSLNSGAAAGEALRQAGYAVTLLDVGRDVAAFIAALTQTRPDV
CCEEEEEEECCCCCCEEECCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCHHH
VFNALHGRFGEDGAIQGILDIMALPYTHSGRLASAIAMDKPSAKLVFERFAIPVAPHVVA
HHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCHHHH
DRASVLAETAMARPYVVKPLDQGSSVGVTIVTSETNDLPFSRDDWPYGRQVMVERFIPGR
HHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCC
ELTVGVMGDKALGVTEIVTDHRFYDYDAKYAQGGSRHIVPAPVAPEVFAQAQELSVLAHQ
EEEEEECCCCCCCHHHHHHCCEEECCCCHHHCCCCCEEECCCCCHHHHHHHHHHHHHHHH
ALGCRGVSRADLRFDGETLYMLEVNTQPGMTDTSLVPEQALHAGISFPDLVTWLVETAQC
HHCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCC
DA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA