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The map label for this gene is metK1

Identifier: 83592253

GI number: 83592253

Start: 1090746

End: 1091897

Strand: Direct

Name: metK1

Synonym: Rru_A0917

Alternate gene names: 83592253

Gene position: 1090746-1091897 (Clockwise)

Preceding gene: 83592252

Following gene: 83592254

Centisome position: 25.06

GC content: 65.19

Gene sequence:

>1152_bases
ATGCGTGCATATTGCGTGACTTCGGAATCAGTTACGGAAGGCCACCCCGACAAGGTTTGTGACCAGATTTCCGACGGCAT
CCTTGATGCCTGTCTGGCGCAGGATCCGGCGGCGCGGGTGGCGGTGGAGACCCTGGTTTCGGGCAACACCGTTTTTATCG
CCGGTGAGATAACCACAACGGCGCGCCTGGACGCGGTGCGCACGGCACGGGAGGTAATCGAAGATATCGGCTATGCCGAT
CCGGCCCTTGGCTTCGACAGCGGGAGCTGCTTCATCCTGACCAATTTGCGCACCCAGTCCCCCGATATCGACCTTGGCGT
CTCGCGCGGCGCCGAATTGGGCGCGGGCGATCAGGGCGTGCTCTACGGCTATGCCTGCGATGAGACGGCGAGCCTTATGC
CCGCCCCCATTCACATGGCCCATGGTTTGTCCCTGCGTTTGGCGGCGGCCCGCCATGGGGGGGTGCTGCCCTGGCTGCGC
CCCGACGGCAAGACCCAGGTGACGGTGCGCTACGACGGCGAGGGCCGCCCCGAGGCCCTGACCAGCGTCATCGTCTCGAC
CCAGCATGACGAGGGGGTGGACCGGGAAACCCTGGTGCGCGGCATCATCGAAGCGGTGATCTATCCCGAGGTCGGTGGCT
GGCTGAAGCCCGAGACGCGGGTGCTGATCAACCCGACCGGGCGCTTCGTGATCGGCGGTCCGGCCGGCGATACCGGGGTG
ACCGGCCGCAAGCTAATGGTCGATACCTATGGCGGCTGCGCCCGGCACGGCGGCGGGGCGTTCTCGGGCAAGGATTCGAC
CAAGGTCGATCGCACGGCGGCCTATATGGCGCGCTATGCGGCCAAGAACGTGGTGGCGGCGGGACTGGCCCGGCGCTGCG
AGGTCGCCCTGGCCTATGCCATCGGCGAGCGTTTGCCTGAAATGGTCTCGGTCGAGACCTTCGGAACGGAGACCATCGAT
GTCGACCGCCTGACGGCGGCGGTCCGTGAAACCTTTCCCTTCTCGGTTTCGGGGATGATCGCAGCCCTCGATCTGCGCCG
GCCGATCTTTCGCAAGACGGCGGCATATGGGCATTTTGGTAGGGAAAACAAAGGCTTCCAATGGGAGAAGACCGACCGTG
TTGACGCCTTGCGCGCTGTTTGCGGGCTGTGA

Upstream 100 bases:

>100_bases
GTTGAAGGGTGTCGTTGAAGGCCTAGGGTTGCGTTGCGTGATCGGCGGCTAAGAATACTGGTCTGTTAGCGTTGATGAAT
CATTGGACTTGGAGGGGTGG

Downstream 100 bases:

>100_bases
GTTGATCTGATCGGACGGGGCTGGACCTGGAGGAGCCAAAACAAAAAAGAGGATGCGGGGAATGAACGCGTTGCGCCTTG
AAGATCTGATCAGCGTGGAA

Product: s-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase 1; MAT 1; Methionine adenosyltransferase 1

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRAYCVTSESVTEGHPDKVCDQISDGILDACLAQDPAARVAVETLVSGNTVFIAGEITTTARLDAVRTAREVIEDIGYAD
PALGFDSGSCFILTNLRTQSPDIDLGVSRGAELGAGDQGVLYGYACDETASLMPAPIHMAHGLSLRLAAARHGGVLPWLR
PDGKTQVTVRYDGEGRPEALTSVIVSTQHDEGVDRETLVRGIIEAVIYPEVGGWLKPETRVLINPTGRFVIGGPAGDTGV
TGRKLMVDTYGGCARHGGGAFSGKDSTKVDRTAAYMARYAAKNVVAAGLARRCEVALAYAIGERLPEMVSVETFGTETID
VDRLTAAVRETFPFSVSGMIAALDLRRPIFRKTAAYGHFGRENKGFQWEKTDRVDALRAVCGL

Sequences:

>Translated_383_residues
MRAYCVTSESVTEGHPDKVCDQISDGILDACLAQDPAARVAVETLVSGNTVFIAGEITTTARLDAVRTAREVIEDIGYAD
PALGFDSGSCFILTNLRTQSPDIDLGVSRGAELGAGDQGVLYGYACDETASLMPAPIHMAHGLSLRLAAARHGGVLPWLR
PDGKTQVTVRYDGEGRPEALTSVIVSTQHDEGVDRETLVRGIIEAVIYPEVGGWLKPETRVLINPTGRFVIGGPAGDTGV
TGRKLMVDTYGGCARHGGGAFSGKDSTKVDRTAAYMARYAAKNVVAAGLARRCEVALAYAIGERLPEMVSVETFGTETID
VDRLTAAVRETFPFSVSGMIAALDLRRPIFRKTAAYGHFGRENKGFQWEKTDRVDALRAVCGL
>Mature_383_residues
MRAYCVTSESVTEGHPDKVCDQISDGILDACLAQDPAARVAVETLVSGNTVFIAGEITTTARLDAVRTAREVIEDIGYAD
PALGFDSGSCFILTNLRTQSPDIDLGVSRGAELGAGDQGVLYGYACDETASLMPAPIHMAHGLSLRLAAARHGGVLPWLR
PDGKTQVTVRYDGEGRPEALTSVIVSTQHDEGVDRETLVRGIIEAVIYPEVGGWLKPETRVLINPTGRFVIGGPAGDTGV
TGRKLMVDTYGGCARHGGGAFSGKDSTKVDRTAAYMARYAAKNVVAAGLARRCEVALAYAIGERLPEMVSVETFGTETID
VDRLTAAVRETFPFSVSGMIAALDLRRPIFRKTAAYGHFGRENKGFQWEKTDRVDALRAVCGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=373, Percent_Identity=50.402144772118, Blast_Score=339, Evalue=2e-93,
Organism=Homo sapiens, GI5174529, Length=376, Percent_Identity=49.468085106383, Blast_Score=335, Evalue=3e-92,
Organism=Escherichia coli, GI1789311, Length=386, Percent_Identity=55.440414507772, Blast_Score=429, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551082, Length=374, Percent_Identity=48.9304812834225, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI25145633, Length=378, Percent_Identity=47.8835978835979, Blast_Score=330, Evalue=8e-91,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=48.1283422459893, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17538494, Length=376, Percent_Identity=47.0744680851064, Blast_Score=323, Evalue=7e-89,
Organism=Caenorhabditis elegans, GI32565905, Length=379, Percent_Identity=43.0079155672823, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI32565911, Length=327, Percent_Identity=45.8715596330275, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI25145635, Length=331, Percent_Identity=45.619335347432, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI32565903, Length=304, Percent_Identity=45.3947368421053, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI32565909, Length=91, Percent_Identity=49.4505494505495, Blast_Score=94, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6320710, Length=384, Percent_Identity=48.1770833333333, Blast_Score=345, Evalue=5e-96,
Organism=Saccharomyces cerevisiae, GI6323209, Length=377, Percent_Identity=49.0716180371353, Blast_Score=335, Evalue=7e-93,
Organism=Drosophila melanogaster, GI45552159, Length=374, Percent_Identity=47.5935828877005, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580529, Length=374, Percent_Identity=47.5935828877005, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580537, Length=374, Percent_Identity=47.5935828877005, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580533, Length=374, Percent_Identity=47.5935828877005, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580535, Length=374, Percent_Identity=47.5935828877005, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580531, Length=374, Percent_Identity=47.5935828877005, Blast_Score=322, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24580527, Length=374, Percent_Identity=47.0588235294118, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24580525, Length=374, Percent_Identity=47.0588235294118, Blast_Score=317, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24580541, Length=361, Percent_Identity=46.2603878116344, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=49.1525423728814, Blast_Score=111, Evalue=8e-25,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK1_RHORT (Q2RVX7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426005.1
- ProteinModelPortal:   Q2RVX7
- SMR:   Q2RVX7
- STRING:   Q2RVX7
- GeneID:   3833953
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0917
- NMPDR:   fig|1085.1.peg.3455
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   RTAAYMA
- PhylomeDB:   Q2RVX7
- ProtClustDB:   PRK12459
- BioCyc:   RRUB269796:RRU_A0917-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 40868; Mature: 40868

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAYCVTSESVTEGHPDKVCDQISDGILDACLAQDPAARVAVETLVSGNTVFIAGEITTT
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCH
ARLDAVRTAREVIEDIGYADPALGFDSGSCFILTNLRTQSPDIDLGVSRGAELGAGDQGV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCE
LYGYACDETASLMPAPIHMAHGLSLRLAAARHGGVLPWLRPDGKTQVTVRYDGEGRPEAL
EEEEECCCCCCCCCCCHHHHCCCEEEEEEHHCCCCCCEECCCCCEEEEEEECCCCCHHHH
TSVIVSTQHDEGVDRETLVRGIIEAVIYPEVGGWLKPETRVLINPTGRFVIGGPAGDTGV
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCC
TGRKLMVDTYGGCARHGGGAFSGKDSTKVDRTAAYMARYAAKNVVAAGLARRCEVALAYA
CCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGERLPEMVSVETFGTETIDVDRLTAAVRETFPFSVSGMIAALDLRRPIFRKTAAYGHFG
HHHHCHHHEEEECCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RENKGFQWEKTDRVDALRAVCGL
CCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRAYCVTSESVTEGHPDKVCDQISDGILDACLAQDPAARVAVETLVSGNTVFIAGEITTT
CCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCH
ARLDAVRTAREVIEDIGYADPALGFDSGSCFILTNLRTQSPDIDLGVSRGAELGAGDQGV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCE
LYGYACDETASLMPAPIHMAHGLSLRLAAARHGGVLPWLRPDGKTQVTVRYDGEGRPEAL
EEEEECCCCCCCCCCCHHHHCCCEEEEEEHHCCCCCCEECCCCCEEEEEEECCCCCHHHH
TSVIVSTQHDEGVDRETLVRGIIEAVIYPEVGGWLKPETRVLINPTGRFVIGGPAGDTGV
HHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEEECCCCCCCC
TGRKLMVDTYGGCARHGGGAFSGKDSTKVDRTAAYMARYAAKNVVAAGLARRCEVALAYA
CCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGERLPEMVSVETFGTETIDVDRLTAAVRETFPFSVSGMIAALDLRRPIFRKTAAYGHFG
HHHHCHHHEEEECCCCCEECHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
RENKGFQWEKTDRVDALRAVCGL
CCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA