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The map label for this gene is ptsP [H]

Identifier: 83592084

GI number: 83592084

Start: 870988

End: 873255

Strand: Direct

Name: ptsP [H]

Synonym: Rru_A0745

Alternate gene names: 83592084

Gene position: 870988-873255 (Clockwise)

Preceding gene: 83592083

Following gene: 83592085

Centisome position: 20.01

GC content: 66.62

Gene sequence:

>2268_bases
ATGCCTGAGCGGGAACGCGACGCACGCAGCCTGCTTGGTCGTCTGCGCGATATCATGGCCGGTGGCGGGTCGCCGCAGGT
CCGCCTTGACCTTGTCACGGCCTTGATCGCGCGCGGCCTGGGGACCGATGTCTGTTCGTGCTACGTGCTGCGCGCCGGCG
AGGTTCTGGAGCTTTTCGCCACCGAGGGCCTGTCGCAGGCCGCCGTTCACAAGACGCGACTGCGGGTTGGCGAAGGGCTG
ATCGGCGAGGTGGCGGCCACCGGCCGCGCCCTGACCGTGGCCGACGCCTGGTCCCACCCCAATTTCGCCTATCGCCCGGA
AACCGGGGAAGATCTGTTCCAGTCGCTGATGGGCGTGCCGATGATACGCGGCGGCCGGGTGATCGGCGTTCTGGCCGTGC
AAAATCGCGAGTCGCGTGACTACGAGGAGTTCGAGCAGGAAATCCTCGAAACCGTCGCCATGGTTCTGGCCGAGATGCTG
GCCGGGGCCGATCTGGTGGGCAAGGACGAACTGGGGCCGGTGGCGGGCAATGCCGTGCTGCCGATGCGCCTGGAGGGCGA
TCGCCTGAACGGCGGCATCGGCCTGGGAACCGCCGTCTATCATCGGCCCGAGGTGATGGTCCACCGGCTGGTTGGCGACG
ATCCCGAAACCGAAATGGAGCGCCTGCGCACGGCGCTGTCGGAAATGCGCCAGAGCATCGACAGCATGCTGGCCCTGCAC
CGCGACGTTCCGGGCGAATATCACGACGTTCTGGAAACCTTCCGCATGTTCGCCGAGGATGCGGGCTGGATCGGTCGTAT
CTCCGAGCATATCCAGACCGGGCTGTCGGCCGAGGGCGCGGTGCGCAAGGTCGATGATGACATGCGCCTGCGCATGTCCC
AGGTCAGCGATCCCTATATCCGCGAACGCCTTCACGACCTCGAGGATCTGGCCAACCGCCTGCTGCGCCATCTGACCGGC
GACGGCTCGACCGCCGCGCTCAGCGACCTGCCCGAGCATGTCGTGCTGATCTGCCGCAGCCTGGGGCCGGCCGAACTCCT
TGACTATGACCCCTCGCGCCTGCGCGGGGTGATCATGGAGGAGGGCAGTTCGGGCATGCATGCGGTGATCGTGGCCAAGG
CGCTCGATATTCCGGTGATCGGCCGGGTACCCGATGTCTTCCAGCGCGTCGCTCCGCTTGATCAGGTGGTGGTCGATGGC
GATCACGGTCATGTTTTCGTCCGCCCCGGCGAAGACGTGCGCGAAGCGTTCCTTAATGGCGTGCTGGCGCGCAAGCAGCG
CCGCGCCTTCTATCAAAGCTTCCGCGACCAGCCCGCCGTCAGCCGCGACGGCTATCGCATCAAATTGATGATCAACGCCG
GCCTGCTGTTCGATATGGAGGGGCTGGTCGAAAGCGGGGCCGACGGGGTGGGGCTTTATCGCACCGAGATCCCCTTCATG
GCCCGGCCGTCGCTGCCCAGCGTCGAGACCCAGACCGAGCTTTACGACAAGGTTCTGGAACTGGCCGGCGACAAGCCGGT
CACCTTCCGCACCCTTGATGTCGGCGGCGACAAGCTGCTGCCCTATTGGAACCGCGCGCCCGAAGACAATCCGGCGATGG
GCTGGCGCTCGATCCGCATCACCCTTGACCGGCCGGCGATCCTGCGCGAGCAGTTGCGCGCCCTGATCCGCGCCTCGGGA
CAGCGGGAATTGCGCGTCATGTTCCCGATGGTCGCCAACGTCGCCGAGTTCCGCGCCGCCCGCAAGGTGCTGATGGCCGA
ACTGGCGCGCGAGGCGGCGCGCGGCACGCCGCCGCCCCGGCGGGTGCGCGTTGGAACCATGCTCGAGGTTCCCTCGTTGA
TCTGGCAACTGCCGGCCCTGCTCAAGGATGGCGTCGATTTCATCTCGGTGGGCAGCAATGATCTGATGCAGTTCACCTTT
GCCGCCGATCGCGCCAATTCGCGGCTGGCCGGGCGCTATGATCCGCTGTCGCCGGCCGCGCTGTCGATCCTGCATCGGGT
GGTCACGCTCTGCGACGCCGCCGCCGTGCCGCTGTCGCTGTGTGGCGAGATGGCCTCGCGGCCGCTTGACGCCATGGCCC
TGGTGGCCCTGGGCTTCCGCTTCCTGTCGGTGGCGGCGCCGGGGGTCGGGCCGGTCAAGCAGATGCTGCTGTCGACCAAT
ATCGATCAGGTCCGTCCCTATGTGGAAACCCTGCTGGATTTGCCCGACGCCTCGGTCCGCGAAAACCTGCGCTCCTTCGC
CCTTGACCACGGTATTTCCCTGTCGTAA

Upstream 100 bases:

>100_bases
GAAATACTGGCCGAATTGCGGGGACAGGCCGTCGCGGCCCTGACCCGCAGGGCCGCCCGGCAAGCGGGTGGTTGAGCGTA
CGAAAGGGGGGCATCGGGAG

Downstream 100 bases:

>100_bases
GGCAGCCGGGCGCCGCGATCGTTGCGGAAACGAGCGTTGTTCCCAGAGGGGTTTTTCGCTAGGTTCGTGGCGTCCGCGCC
GGATTGCCGCCGGGGGAGAA

Product: phosphoenolpyruvate-protein phosphotransferase PtsP

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MPERERDARSLLGRLRDIMAGGGSPQVRLDLVTALIARGLGTDVCSCYVLRAGEVLELFATEGLSQAAVHKTRLRVGEGL
IGEVAATGRALTVADAWSHPNFAYRPETGEDLFQSLMGVPMIRGGRVIGVLAVQNRESRDYEEFEQEILETVAMVLAEML
AGADLVGKDELGPVAGNAVLPMRLEGDRLNGGIGLGTAVYHRPEVMVHRLVGDDPETEMERLRTALSEMRQSIDSMLALH
RDVPGEYHDVLETFRMFAEDAGWIGRISEHIQTGLSAEGAVRKVDDDMRLRMSQVSDPYIRERLHDLEDLANRLLRHLTG
DGSTAALSDLPEHVVLICRSLGPAELLDYDPSRLRGVIMEEGSSGMHAVIVAKALDIPVIGRVPDVFQRVAPLDQVVVDG
DHGHVFVRPGEDVREAFLNGVLARKQRRAFYQSFRDQPAVSRDGYRIKLMINAGLLFDMEGLVESGADGVGLYRTEIPFM
ARPSLPSVETQTELYDKVLELAGDKPVTFRTLDVGGDKLLPYWNRAPEDNPAMGWRSIRITLDRPAILREQLRALIRASG
QRELRVMFPMVANVAEFRAARKVLMAELAREAARGTPPPRRVRVGTMLEVPSLIWQLPALLKDGVDFISVGSNDLMQFTF
AADRANSRLAGRYDPLSPAALSILHRVVTLCDAAAVPLSLCGEMASRPLDAMALVALGFRFLSVAAPGVGPVKQMLLSTN
IDQVRPYVETLLDLPDASVRENLRSFALDHGISLS

Sequences:

>Translated_755_residues
MPERERDARSLLGRLRDIMAGGGSPQVRLDLVTALIARGLGTDVCSCYVLRAGEVLELFATEGLSQAAVHKTRLRVGEGL
IGEVAATGRALTVADAWSHPNFAYRPETGEDLFQSLMGVPMIRGGRVIGVLAVQNRESRDYEEFEQEILETVAMVLAEML
AGADLVGKDELGPVAGNAVLPMRLEGDRLNGGIGLGTAVYHRPEVMVHRLVGDDPETEMERLRTALSEMRQSIDSMLALH
RDVPGEYHDVLETFRMFAEDAGWIGRISEHIQTGLSAEGAVRKVDDDMRLRMSQVSDPYIRERLHDLEDLANRLLRHLTG
DGSTAALSDLPEHVVLICRSLGPAELLDYDPSRLRGVIMEEGSSGMHAVIVAKALDIPVIGRVPDVFQRVAPLDQVVVDG
DHGHVFVRPGEDVREAFLNGVLARKQRRAFYQSFRDQPAVSRDGYRIKLMINAGLLFDMEGLVESGADGVGLYRTEIPFM
ARPSLPSVETQTELYDKVLELAGDKPVTFRTLDVGGDKLLPYWNRAPEDNPAMGWRSIRITLDRPAILREQLRALIRASG
QRELRVMFPMVANVAEFRAARKVLMAELAREAARGTPPPRRVRVGTMLEVPSLIWQLPALLKDGVDFISVGSNDLMQFTF
AADRANSRLAGRYDPLSPAALSILHRVVTLCDAAAVPLSLCGEMASRPLDAMALVALGFRFLSVAAPGVGPVKQMLLSTN
IDQVRPYVETLLDLPDASVRENLRSFALDHGISLS
>Mature_754_residues
PERERDARSLLGRLRDIMAGGGSPQVRLDLVTALIARGLGTDVCSCYVLRAGEVLELFATEGLSQAAVHKTRLRVGEGLI
GEVAATGRALTVADAWSHPNFAYRPETGEDLFQSLMGVPMIRGGRVIGVLAVQNRESRDYEEFEQEILETVAMVLAEMLA
GADLVGKDELGPVAGNAVLPMRLEGDRLNGGIGLGTAVYHRPEVMVHRLVGDDPETEMERLRTALSEMRQSIDSMLALHR
DVPGEYHDVLETFRMFAEDAGWIGRISEHIQTGLSAEGAVRKVDDDMRLRMSQVSDPYIRERLHDLEDLANRLLRHLTGD
GSTAALSDLPEHVVLICRSLGPAELLDYDPSRLRGVIMEEGSSGMHAVIVAKALDIPVIGRVPDVFQRVAPLDQVVVDGD
HGHVFVRPGEDVREAFLNGVLARKQRRAFYQSFRDQPAVSRDGYRIKLMINAGLLFDMEGLVESGADGVGLYRTEIPFMA
RPSLPSVETQTELYDKVLELAGDKPVTFRTLDVGGDKLLPYWNRAPEDNPAMGWRSIRITLDRPAILREQLRALIRASGQ
RELRVMFPMVANVAEFRAARKVLMAELAREAARGTPPPRRVRVGTMLEVPSLIWQLPALLKDGVDFISVGSNDLMQFTFA
ADRANSRLAGRYDPLSPAALSILHRVVTLCDAAAVPLSLCGEMASRPLDAMALVALGFRFLSVAAPGVGPVKQMLLSTNI
DQVRPYVETLLDLPDASVRENLRSFALDHGISLS

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=724, Percent_Identity=34.2541436464088, Blast_Score=351, Evalue=8e-98,
Organism=Escherichia coli, GI1788756, Length=551, Percent_Identity=31.9419237749546, Blast_Score=290, Evalue=3e-79,
Organism=Escherichia coli, GI1788726, Length=543, Percent_Identity=30.7550644567219, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI48994992, Length=479, Percent_Identity=30.062630480167, Blast_Score=229, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 82925; Mature: 82793

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERERDARSLLGRLRDIMAGGGSPQVRLDLVTALIARGLGTDVCSCYVLRAGEVLELFA
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHH
TEGLSQAAVHKTRLRVGEGLIGEVAATGRALTVADAWSHPNFAYRPETGEDLFQSLMGVP
HCCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCC
MIRGGRVIGVLAVQNRESRDYEEFEQEILETVAMVLAEMLAGADLVGKDELGPVAGNAVL
EECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
PMRLEGDRLNGGIGLGTAVYHRPEVMVHRLVGDDPETEMERLRTALSEMRQSIDSMLALH
EEEECCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDVPGEYHDVLETFRMFAEDAGWIGRISEHIQTGLSAEGAVRKVDDDMRLRMSQVSDPYI
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCHHH
RERLHDLEDLANRLLRHLTGDGSTAALSDLPEHVVLICRSLGPAELLDYDPSRLRGVIME
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHEE
EGSSGMHAVIVAKALDIPVIGRVPDVFQRVAPLDQVVVDGDHGHVFVRPGEDVREAFLNG
CCCCCCEEEEEEHHCCCCCCCCCHHHHHHHCCHHHEEEECCCCEEEEECCHHHHHHHHHH
VLARKQRRAFYQSFRDQPAVSRDGYRIKLMINAGLLFDMEGLVESGADGVGLYRTEIPFM
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEHHHHHHCCCCCCEEEEECCCCC
ARPSLPSVETQTELYDKVLELAGDKPVTFRTLDVGGDKLLPYWNRAPEDNPAMGWRSIRI
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
TLDRPAILREQLRALIRASGQRELRVMFPMVANVAEFRAARKVLMAELAREAARGTPPPR
EECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVRVGTMLEVPSLIWQLPALLKDGVDFISVGSNDLMQFTFAADRANSRLAGRYDPLSPAA
CEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCHHEEEEEHHHCCCCCCCCCCCCCCHHH
LSILHRVVTLCDAAAVPLSLCGEMASRPLDAMALVALGFRFLSVAAPGVGPVKQMLLSTN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
IDQVRPYVETLLDLPDASVRENLRSFALDHGISLS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PERERDARSLLGRLRDIMAGGGSPQVRLDLVTALIARGLGTDVCSCYVLRAGEVLELFA
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHH
TEGLSQAAVHKTRLRVGEGLIGEVAATGRALTVADAWSHPNFAYRPETGEDLFQSLMGVP
HCCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCC
MIRGGRVIGVLAVQNRESRDYEEFEQEILETVAMVLAEMLAGADLVGKDELGPVAGNAVL
EECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
PMRLEGDRLNGGIGLGTAVYHRPEVMVHRLVGDDPETEMERLRTALSEMRQSIDSMLALH
EEEECCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RDVPGEYHDVLETFRMFAEDAGWIGRISEHIQTGLSAEGAVRKVDDDMRLRMSQVSDPYI
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHCCCHHH
RERLHDLEDLANRLLRHLTGDGSTAALSDLPEHVVLICRSLGPAELLDYDPSRLRGVIME
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHEE
EGSSGMHAVIVAKALDIPVIGRVPDVFQRVAPLDQVVVDGDHGHVFVRPGEDVREAFLNG
CCCCCCEEEEEEHHCCCCCCCCCHHHHHHHCCHHHEEEECCCCEEEEECCHHHHHHHHHH
VLARKQRRAFYQSFRDQPAVSRDGYRIKLMINAGLLFDMEGLVESGADGVGLYRTEIPFM
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEHHHHHHCCCCCCEEEEECCCCC
ARPSLPSVETQTELYDKVLELAGDKPVTFRTLDVGGDKLLPYWNRAPEDNPAMGWRSIRI
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
TLDRPAILREQLRALIRASGQRELRVMFPMVANVAEFRAARKVLMAELAREAARGTPPPR
EECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RVRVGTMLEVPSLIWQLPALLKDGVDFISVGSNDLMQFTFAADRANSRLAGRYDPLSPAA
CEEECHHHHHHHHHHHHHHHHHCCCCEEECCCCHHEEEEEHHHCCCCCCCCCCCCCCHHH
LSILHRVVTLCDAAAVPLSLCGEMASRPLDAMALVALGFRFLSVAAPGVGPVKQMLLSTN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
IDQVRPYVETLLDLPDASVRENLRSFALDHGISLS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]