Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 83592028

GI number: 83592028

Start: 814140

End: 815519

Strand: Direct

Name: cysS

Synonym: Rru_A0689

Alternate gene names: 83592028

Gene position: 814140-815519 (Clockwise)

Preceding gene: 83592027

Following gene: 83592029

Centisome position: 18.7

GC content: 66.3

Gene sequence:

>1380_bases
GTGACGCTTCATATCCATAACACCATGACCCGCACCAAGGAGGTTTTCGAACCTCTCGATCCCGGCCATGTGCGGCTCTA
TGTCTGCGGTCCAACGGTTTATGACCGGGCCCATATCGGCAATGCCCGGCCGGTCATCGTTTTCGATCTGCTGGCCCGCT
TGCTGCGCCGGCTTTATCCCCAGGTCACCTACGTTCGCAACATCACCGACGTTGACGACAAGATCAACGCCCGCGCCAGC
GCCAGCGGCCGCACCATCGGCGAGATCACCGAGGAGACCACCCGGCTGTTCCACGAGGATATGGCCGAATTGGGCGCGCT
GCCCCCCGATGTCGAACCGCGCGCCACCGCCCATATCGCCGATATGGTGGCGATGATCGAGCGGCTGATCGCCAAGGGCC
ACGCCTATGAGGCCGAGGGCCATGTGCTGTTCAGCGTGCCGAGCATGGGCGCCTATGGCTCGCTGTCGGGCCGGTCGATG
GACGACATGATCGCCGGAGCCCGGGTCGAGGTGGCGCCCTATAAGCGCGATCCCGCCGATTTCGTGCTGTGGAAGCCCTC
GGACGCCAGCCTACCCGGCTGGGACAGCCCCTGGGGGCGCGGCCGGCCGGGCTGGCATATCGAATGCTCGGCGATGTCGT
CGCGCTATCTGGGGCCGTCGTTCGATATTCACGGCGGCGGCTTGGATCTGATCTTCCCCCATCACGAAAACGAGATCGCC
CAAAGCGTCTGCTGCAATGGTCCGGGAACCTTCGCCCGCTACTGGATGCACAACGGCTATCTGATGGTCGAAGGCGAGAA
GATGTCCAAATCCCTGGGCAACTTCGTCACCGTGCGCGACCTGCTTGATCAGGCGCCGGGCGAGGCCATGCGGCTGGCCA
TGCTCGGCACCCATTACCGCCAGCCCTTCGACTGGACGGCCGAGGGGCTGGAGCAGGCGCGGCGCGGCCTGGATCGCCTC
TATTCGGCGCTGCGCCGGGTCGCCGGGATCGCCGCCTCGCCGGCCGAGGTTCCCGAAGGGGTGATGGCCGCGCTTTGTGA
TGATCTTAATACCCCCAAGGCCCTGGCCGAGGTCTACGACCTGCTCGGCGTCCTCAACCGGGCGACCACCACCGAAGAGC
AGGCGAAGGCCAAGGGTGCCCTGCTGGCGGCGGGCGCGCTTCTCGGCCTGTTCCAGGCCGATCCCGAGGTTTGGTTGTGC
GGGGCGGGGACCGGCGAGGAGGAGGGGCTGGCCGCCCGCGTCGAAGATCTGATCTGTCAGCGCAAGGCCGCCCGCCAGGC
CCGCGACTTCGCCCGCGCCGATGCCATCCGCGACGAACTGACCCAGGCCGGGATCGTGCTGGAAGACGGACCGAACGGCA
CCACTTGGCGAAAAGCCTAA

Upstream 100 bases:

>100_bases
GCGACAAGGCCCTTGCCCGGCTGCGTGGCGAAAAGGCATAACCCGGGGCGATCGACAAGTCCCCGCCCGGTTCGAGCCGG
GAAGCAAGGAACCGAGACCG

Downstream 100 bases:

>100_bases
GCTGCGGACTCCTTCTTTTCGCGGGAAGATGATAGCCTGATCCATCCTTAGGGCTACACCGCTTGGGCGGTGTGGCCGGA
TCGGGAGGATCGGATGGCCC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTLHIHNTMTRTKEVFEPLDPGHVRLYVCGPTVYDRAHIGNARPVIVFDLLARLLRRLYPQVTYVRNITDVDDKINARAS
ASGRTIGEITEETTRLFHEDMAELGALPPDVEPRATAHIADMVAMIERLIAKGHAYEAEGHVLFSVPSMGAYGSLSGRSM
DDMIAGARVEVAPYKRDPADFVLWKPSDASLPGWDSPWGRGRPGWHIECSAMSSRYLGPSFDIHGGGLDLIFPHHENEIA
QSVCCNGPGTFARYWMHNGYLMVEGEKMSKSLGNFVTVRDLLDQAPGEAMRLAMLGTHYRQPFDWTAEGLEQARRGLDRL
YSALRRVAGIAASPAEVPEGVMAALCDDLNTPKALAEVYDLLGVLNRATTTEEQAKAKGALLAAGALLGLFQADPEVWLC
GAGTGEEEGLAARVEDLICQRKAARQARDFARADAIRDELTQAGIVLEDGPNGTTWRKA

Sequences:

>Translated_459_residues
MTLHIHNTMTRTKEVFEPLDPGHVRLYVCGPTVYDRAHIGNARPVIVFDLLARLLRRLYPQVTYVRNITDVDDKINARAS
ASGRTIGEITEETTRLFHEDMAELGALPPDVEPRATAHIADMVAMIERLIAKGHAYEAEGHVLFSVPSMGAYGSLSGRSM
DDMIAGARVEVAPYKRDPADFVLWKPSDASLPGWDSPWGRGRPGWHIECSAMSSRYLGPSFDIHGGGLDLIFPHHENEIA
QSVCCNGPGTFARYWMHNGYLMVEGEKMSKSLGNFVTVRDLLDQAPGEAMRLAMLGTHYRQPFDWTAEGLEQARRGLDRL
YSALRRVAGIAASPAEVPEGVMAALCDDLNTPKALAEVYDLLGVLNRATTTEEQAKAKGALLAAGALLGLFQADPEVWLC
GAGTGEEEGLAARVEDLICQRKAARQARDFARADAIRDELTQAGIVLEDGPNGTTWRKA
>Mature_458_residues
TLHIHNTMTRTKEVFEPLDPGHVRLYVCGPTVYDRAHIGNARPVIVFDLLARLLRRLYPQVTYVRNITDVDDKINARASA
SGRTIGEITEETTRLFHEDMAELGALPPDVEPRATAHIADMVAMIERLIAKGHAYEAEGHVLFSVPSMGAYGSLSGRSMD
DMIAGARVEVAPYKRDPADFVLWKPSDASLPGWDSPWGRGRPGWHIECSAMSSRYLGPSFDIHGGGLDLIFPHHENEIAQ
SVCCNGPGTFARYWMHNGYLMVEGEKMSKSLGNFVTVRDLLDQAPGEAMRLAMLGTHYRQPFDWTAEGLEQARRGLDRLY
SALRRVAGIAASPAEVPEGVMAALCDDLNTPKALAEVYDLLGVLNRATTTEEQAKAKGALLAAGALLGLFQADPEVWLCG
AGTGEEEGLAARVEDLICQRKAARQARDFARADAIRDELTQAGIVLEDGPNGTTWRKA

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=483, Percent_Identity=35.6107660455487, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI10835051, Length=409, Percent_Identity=31.2958435207824, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI21269877, Length=409, Percent_Identity=31.2958435207824, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI303304987, Length=409, Percent_Identity=31.2958435207824, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI62240992, Length=409, Percent_Identity=31.2958435207824, Blast_Score=199, Evalue=5e-51,
Organism=Escherichia coli, GI1786737, Length=462, Percent_Identity=50, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17509797, Length=433, Percent_Identity=30.2540415704388, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17509795, Length=433, Percent_Identity=30.2540415704388, Blast_Score=171, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6324082, Length=381, Percent_Identity=30.4461942257218, Blast_Score=196, Evalue=9e-51,
Organism=Drosophila melanogaster, GI19922218, Length=501, Percent_Identity=32.9341317365269, Blast_Score=231, Evalue=6e-61,
Organism=Drosophila melanogaster, GI19922364, Length=309, Percent_Identity=36.8932038834951, Blast_Score=203, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_RHORT (Q2RWK2)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_425780.1
- ProteinModelPortal:   Q2RWK2
- SMR:   Q2RWK2
- STRING:   Q2RWK2
- GeneID:   3833926
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0689
- NMPDR:   fig|1085.1.peg.1655
- eggNOG:   COG0215
- HOGENOM:   HBG327651
- OMA:   PEEMLAG
- PhylomeDB:   Q2RWK2
- ProtClustDB:   PRK00260
- BioCyc:   RRUB269796:RRU_A0689-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 50262; Mature: 50130

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 270-270

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLHIHNTMTRTKEVFEPLDPGHVRLYVCGPTVYDRAHIGNARPVIVFDLLARLLRRLYP
CEEEECHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHCC
QVTYVRNITDVDDKINARASASGRTIGEITEETTRLFHEDMAELGALPPDVEPRATAHIA
HHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
DMVAMIERLIAKGHAYEAEGHVLFSVPSMGAYGSLSGRSMDDMIAGARVEVAPYKRDPAD
HHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCC
FVLWKPSDASLPGWDSPWGRGRPGWHIECSAMSSRYLGPSFDIHGGGLDLIFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEECCCHHHHH
QSVCCNGPGTFARYWMHNGYLMVEGEKMSKSLGNFVTVRDLLDQAPGEAMRLAMLGTHYR
HHHHCCCCCHHHHHHHCCCEEEEECHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCC
QPFDWTAEGLEQARRGLDRLYSALRRVAGIAASPAEVPEGVMAALCDDLNTPKALAEVYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHCCCCHHHHHHHHH
LLGVLNRATTTEEQAKAKGALLAAGALLGLFQADPEVWLCGAGTGEEEGLAARVEDLICQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHH
RKAARQARDFARADAIRDELTQAGIVLEDGPNGTTWRKA
HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure 
TLHIHNTMTRTKEVFEPLDPGHVRLYVCGPTVYDRAHIGNARPVIVFDLLARLLRRLYP
EEEECHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHCC
QVTYVRNITDVDDKINARASASGRTIGEITEETTRLFHEDMAELGALPPDVEPRATAHIA
HHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
DMVAMIERLIAKGHAYEAEGHVLFSVPSMGAYGSLSGRSMDDMIAGARVEVAPYKRDPAD
HHHHHHHHHHHCCCCEECCCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCC
FVLWKPSDASLPGWDSPWGRGRPGWHIECSAMSSRYLGPSFDIHGGGLDLIFPHHENEIA
EEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCEEEEECCCHHHHH
QSVCCNGPGTFARYWMHNGYLMVEGEKMSKSLGNFVTVRDLLDQAPGEAMRLAMLGTHYR
HHHHCCCCCHHHHHHHCCCEEEEECHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCC
QPFDWTAEGLEQARRGLDRLYSALRRVAGIAASPAEVPEGVMAALCDDLNTPKALAEVYD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCHHHHHHHHHHCCCCHHHHHHHHH
LLGVLNRATTTEEQAKAKGALLAAGALLGLFQADPEVWLCGAGTGEEEGLAARVEDLICQ
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHH
RKAARQARDFARADAIRDELTQAGIVLEDGPNGTTWRKA
HHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA