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The map label for this gene is gltX1

Identifier: 83592027

GI number: 83592027

Start: 812746

End: 814080

Strand: Direct

Name: gltX1

Synonym: Rru_A0688

Alternate gene names: 83592027

Gene position: 812746-814080 (Clockwise)

Preceding gene: 83592026

Following gene: 83592028

Centisome position: 18.67

GC content: 69.89

Gene sequence:

>1335_bases
ATGTCGTCCGTTCTCGTGCGTTTCGCCCCCAGCCCCACCGGCCGGTTGCACCTGGGCAATGCCCGCGTCGCCGTTCTGAA
CTGGTTGTTCGCCCATGCCCATGGCGGGGCGATGGTGCTGCGTCTGGATGATACGGATATCGCGCGCGGCACCGCCGAAT
TCGCCCAGGCGATCCGCGATGATCTGCTTTGGCTTGGCCTGACCTGGACGCGCGAGGAAAGCCAACTGGCCCGCGCCGCC
CGCCATGCCGAGGCCGTTGACCAACTGAAGGCGGCCGGCCGGCTCTATGCCTGCTATGAGACCCCCGAAGAGCTGGAATA
CCGCCGCCGCCGCCAGCGCGCCCAGGGGCTGCCGCCGATCTATGACCGCGCCGGTCTGGCTTTGAGCGCGGCCGAGCGCG
CCGCCCTGGAGGCCGAGGGCCGCAAGCCCCATTGGCGCTTCCGCCTTGATCACGAGGAGACCTCGTGGCTGGATCTGGCG
CGCGGCCCCTGCCATGCCCATGGCGGCCATTTGTCCGACCCGGTGCTGGTGCGCGAGGATGGCAGCCTGCTCTACACCCT
GCCGTCGGTGGTCGATGACATCGATTTCGCCATCAGCCATGTCATTCGCGGCGAGGATCATGTGGTCAATACGGCGGTGC
AGATCCAGATCTGCCGGGCCTTGGGGGCCGAGCCGCCGCACTACGCCCATCTGCCGCTGGTGCTGACCGCCGAGGGCGGC
GGGCTGTCCAAGCGCGACAACGCCCTGTCGCTGGGCGATCTGCGGGCGCAAGGCATCGAGCCCGCCGCGATCAACGCCCT
GCTCGCCGCCCTGGGCACCGCCGAGGCGCCCGATCCCCTGAAGTCGTTGGATGAGCTGGCCCAGGGCTTTCGCCTGGATG
CCTTTGGCCGCGCCGCGCCGCGCCTGGATCCGGCCGATCTCGTGCGGCTGTCCGCGCGGATCTACCACGCCCTGTCGCCA
AGCGAGGCGCGGGCGCGCGGCATCGCCGTGTCCGATGCCCTGTGGCTGGCCCTGCGCGCCAATCTGACCACGCTGTCCGA
CCTTGACGAGTTGCTGCCCGTCGTCGAAGGCGAAATCACGCCGCTGATCGCCGAGGAAGACCGGGCGATGATCGATGAAG
CCGCCCGCCTGCTGCCCGAGACGCCGTGGGACGCCACCACCTGGGCGACCTGGACGGCGGCGGTCAAGACCGCGACCGGG
CGCAAGGGCAAGGGGCTGTTCATGCCGCTGCGCCGGGCGTTGACCGGCGTTGACCATGGCCCGGAACTGGCCGCGCTGCT
GCCGCTGATCGGGCGCGACAAGGCCCTTGCCCGGCTGCGTGGCGAAAAGGCATAA

Upstream 100 bases:

>100_bases
CAAGATCAGCGCGCTTGCTTTCGGCCGCGACCGCCGCTATCCGATCACCCACGCCTACGGCGGTTAAGCCGGGGCGTTCC
CGGGCCATCAAGGAACCGTC

Downstream 100 bases:

>100_bases
CCCGGGGCGATCGACAAGTCCCCGCCCGGTTCGAGCCGGGAAGCAAGGAACCGAGACCGGTGACGCTTCATATCCATAAC
ACCATGACCCGCACCAAGGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSSVLVRFAPSPTGRLHLGNARVAVLNWLFAHAHGGAMVLRLDDTDIARGTAEFAQAIRDDLLWLGLTWTREESQLARAA
RHAEAVDQLKAAGRLYACYETPEELEYRRRRQRAQGLPPIYDRAGLALSAAERAALEAEGRKPHWRFRLDHEETSWLDLA
RGPCHAHGGHLSDPVLVREDGSLLYTLPSVVDDIDFAISHVIRGEDHVVNTAVQIQICRALGAEPPHYAHLPLVLTAEGG
GLSKRDNALSLGDLRAQGIEPAAINALLAALGTAEAPDPLKSLDELAQGFRLDAFGRAAPRLDPADLVRLSARIYHALSP
SEARARGIAVSDALWLALRANLTTLSDLDELLPVVEGEITPLIAEEDRAMIDEAARLLPETPWDATTWATWTAAVKTATG
RKGKGLFMPLRRALTGVDHGPELAALLPLIGRDKALARLRGEKA

Sequences:

>Translated_444_residues
MSSVLVRFAPSPTGRLHLGNARVAVLNWLFAHAHGGAMVLRLDDTDIARGTAEFAQAIRDDLLWLGLTWTREESQLARAA
RHAEAVDQLKAAGRLYACYETPEELEYRRRRQRAQGLPPIYDRAGLALSAAERAALEAEGRKPHWRFRLDHEETSWLDLA
RGPCHAHGGHLSDPVLVREDGSLLYTLPSVVDDIDFAISHVIRGEDHVVNTAVQIQICRALGAEPPHYAHLPLVLTAEGG
GLSKRDNALSLGDLRAQGIEPAAINALLAALGTAEAPDPLKSLDELAQGFRLDAFGRAAPRLDPADLVRLSARIYHALSP
SEARARGIAVSDALWLALRANLTTLSDLDELLPVVEGEITPLIAEEDRAMIDEAARLLPETPWDATTWATWTAAVKTATG
RKGKGLFMPLRRALTGVDHGPELAALLPLIGRDKALARLRGEKA
>Mature_443_residues
SSVLVRFAPSPTGRLHLGNARVAVLNWLFAHAHGGAMVLRLDDTDIARGTAEFAQAIRDDLLWLGLTWTREESQLARAAR
HAEAVDQLKAAGRLYACYETPEELEYRRRRQRAQGLPPIYDRAGLALSAAERAALEAEGRKPHWRFRLDHEETSWLDLAR
GPCHAHGGHLSDPVLVREDGSLLYTLPSVVDDIDFAISHVIRGEDHVVNTAVQIQICRALGAEPPHYAHLPLVLTAEGGG
LSKRDNALSLGDLRAQGIEPAAINALLAALGTAEAPDPLKSLDELAQGFRLDAFGRAAPRLDPADLVRLSARIYHALSPS
EARARGIAVSDALWLALRANLTTLSDLDELLPVVEGEITPLIAEEDRAMIDEAARLLPETPWDATTWATWTAAVKTATGR
KGKGLFMPLRRALTGVDHGPELAALLPLIGRDKALARLRGEKA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=363, Percent_Identity=33.6088154269972, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1788743, Length=476, Percent_Identity=33.1932773109244, Blast_Score=207, Evalue=9e-55,
Organism=Escherichia coli, GI226510925, Length=294, Percent_Identity=32.6530612244898, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786895, Length=147, Percent_Identity=34.0136054421769, Blast_Score=72, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=356, Percent_Identity=31.7415730337079, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324540, Length=484, Percent_Identity=26.6528925619835, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6321192, Length=182, Percent_Identity=30.2197802197802, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=29.4238683127572, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE1_RHORT (Q2RWK3)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_425779.1
- ProteinModelPortal:   Q2RWK3
- SMR:   Q2RWK3
- STRING:   Q2RWK3
- GeneID:   3834539
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0688
- NMPDR:   fig|1085.1.peg.1656
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   PHWRFKL
- ProtClustDB:   PRK12558
- BioCyc:   RRUB269796:RRU_A0688-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 48293; Mature: 48161

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 244-244

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVLVRFAPSPTGRLHLGNARVAVLNWLFAHAHGGAMVLRLDDTDIARGTAEFAQAIRD
CCCEEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHHHHHHH
DLLWLGLTWTREESQLARAARHAEAVDQLKAAGRLYACYETPEELEYRRRRQRAQGLPPI
HHEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCC
YDRAGLALSAAERAALEAEGRKPHWRFRLDHEETSWLDLARGPCHAHGGHLSDPVLVRED
HHCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCEEEECC
GSLLYTLPSVVDDIDFAISHVIRGEDHVVNTAVQIQICRALGAEPPHYAHLPLVLTAEGG
CCEEEEHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCEECCEEEEECCC
GLSKRDNALSLGDLRAQGIEPAAINALLAALGTAEAPDPLKSLDELAQGFRLDAFGRAAP
CCCCCCCCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEECCCCCCCC
RLDPADLVRLSARIYHALSPSEARARGIAVSDALWLALRANLTTLSDLDELLPVVEGEIT
CCCHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
PLIAEEDRAMIDEAARLLPETPWDATTWATWTAAVKTATGRKGKGLFMPLRRALTGVDHG
EEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
PELAALLPLIGRDKALARLRGEKA
CHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure 
SSVLVRFAPSPTGRLHLGNARVAVLNWLFAHAHGGAMVLRLDDTDIARGTAEFAQAIRD
CCEEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHCCHHHHHHHHHH
DLLWLGLTWTREESQLARAARHAEAVDQLKAAGRLYACYETPEELEYRRRRQRAQGLPPI
HHEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCC
YDRAGLALSAAERAALEAEGRKPHWRFRLDHEETSWLDLARGPCHAHGGHLSDPVLVRED
HHCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCEEEECC
GSLLYTLPSVVDDIDFAISHVIRGEDHVVNTAVQIQICRALGAEPPHYAHLPLVLTAEGG
CCEEEEHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCCEECCEEEEECCC
GLSKRDNALSLGDLRAQGIEPAAINALLAALGTAEAPDPLKSLDELAQGFRLDAFGRAAP
CCCCCCCCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEECCCCCCCC
RLDPADLVRLSARIYHALSPSEARARGIAVSDALWLALRANLTTLSDLDELLPVVEGEIT
CCCHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
PLIAEEDRAMIDEAARLLPETPWDATTWATWTAAVKTATGRKGKGLFMPLRRALTGVDHG
EEEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCC
PELAALLPLIGRDKALARLRGEKA
CHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA