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The map label for this gene is dagK [H]

Identifier: 83591916

GI number: 83591916

Start: 685999

End: 686970

Strand: Reverse

Name: dagK [H]

Synonym: Rru_A0577

Alternate gene names: 83591916

Gene position: 686970-685999 (Counterclockwise)

Preceding gene: 83591927

Following gene: 83591910

Centisome position: 15.78

GC content: 68.52

Gene sequence:

>972_bases
ATGCGTGTCCTGCCGTTTGCGCCTGCCAACCCCGCTCCGGCCCCCGCCCAGCGGCGGGTGATGATTATCGCCAACCCGGC
TGCGGGTTCCCTGAAGCAGCGGCGCTTGAACCGCACCCTGCTTGGCCTGGAGAAACTGGGCTGCACGGTGGGCTTGCGCG
AAACCGCCAAGCCCGGCGACGCCACGCTGATGGCCCGCGAGGCGGCCCTGGCCGGTAATGTCGATGTGGTGGTCGCCGCC
GGCGGTGACGGCACGATCAACGAGGTTGCCAATGGTTTGGCCGGAAGCGGCGTGGCCCTGGGGGTGATTCCGCTGGGCAC
CGCCAATGTGCTGGCGATCGAGGCCGGGATCCCCCGCGATCCGGGCAAGGCCGCCCAGGTCATCGCCACCGGCGTTCTCC
GCAAGCTGTATCTGGGCGAGGTGCGGGCCAGCGCCGAAACGCCGTTGGCCGGTCCCCGCCGCTTCGTGATGATGGCCGGC
GCCGGCTTCGACGCCCATGTCGTCGATACCGTCGATCTCGCCCTCAAGCGCAAGACCGGCAAGCTGGCCTATGTCTGGTG
TACGGTTCAGCGAGCCTTCAGCTATGATTTCCCGCTTTGCGCCGTCGATATCGACACCCCCGACGGCCGGACGATCCATG
CCGATGTCGCCACCGCCGTGGTCTGCAACGGCAAATATTACGGCGGGCCGTTCATCGCCGCGCCCTTGGCCGACATCTCG
CACCCGACCTTTCAGGTGATCTTGCTCAAAAGCCCGGGCCTGCGCCATGTGGCGCGCTATGCCCTGGCCCTGGCCATGGG
CCGCCTGCCCCGCCTGCCCGATGTCGAGATCATCGAGGCGACGCGCGTGCGCATCGCCCTGCAGGGCGCCCAGCCGCTGC
AAGCCGATGGCGATACGGTCGCCCATATGCCCGTCGATATCGTCATGGCCGCCGAGCCGGTATCCCTGATCGTCCCCGCC
CCCGCCGCCTAG

Upstream 100 bases:

>100_bases
TCCGTCTTTTTTCCTTCATGGACACCCTTGACGGCGGAGCTTACCCACCCCTTTTCTCCTATCCGCCCCGTTGATCCCTT
GACAAGGTCCCCCCTCCCCG

Downstream 100 bases:

>100_bases
ACGGGAACGCGACGCCCAAGACCTCAGCCGGCGCGCAGCGAGCCGACGAAGGCGTCGACGGTCTTGCCAAGCTCGCCGAC
CCGCTGGTGCAGATCGGCGA

Product: hypothetical protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MRVLPFAPANPAPAPAQRRVMIIANPAAGSLKQRRLNRTLLGLEKLGCTVGLRETAKPGDATLMAREAALAGNVDVVVAA
GGDGTINEVANGLAGSGVALGVIPLGTANVLAIEAGIPRDPGKAAQVIATGVLRKLYLGEVRASAETPLAGPRRFVMMAG
AGFDAHVVDTVDLALKRKTGKLAYVWCTVQRAFSYDFPLCAVDIDTPDGRTIHADVATAVVCNGKYYGGPFIAAPLADIS
HPTFQVILLKSPGLRHVARYALALAMGRLPRLPDVEIIEATRVRIALQGAQPLQADGDTVAHMPVDIVMAAEPVSLIVPA
PAA

Sequences:

>Translated_323_residues
MRVLPFAPANPAPAPAQRRVMIIANPAAGSLKQRRLNRTLLGLEKLGCTVGLRETAKPGDATLMAREAALAGNVDVVVAA
GGDGTINEVANGLAGSGVALGVIPLGTANVLAIEAGIPRDPGKAAQVIATGVLRKLYLGEVRASAETPLAGPRRFVMMAG
AGFDAHVVDTVDLALKRKTGKLAYVWCTVQRAFSYDFPLCAVDIDTPDGRTIHADVATAVVCNGKYYGGPFIAAPLADIS
HPTFQVILLKSPGLRHVARYALALAMGRLPRLPDVEIIEATRVRIALQGAQPLQADGDTVAHMPVDIVMAAEPVSLIVPA
PAA
>Mature_323_residues
MRVLPFAPANPAPAPAQRRVMIIANPAAGSLKQRRLNRTLLGLEKLGCTVGLRETAKPGDATLMAREAALAGNVDVVVAA
GGDGTINEVANGLAGSGVALGVIPLGTANVLAIEAGIPRDPGKAAQVIATGVLRKLYLGEVRASAETPLAGPRRFVMMAG
AGFDAHVVDTVDLALKRKTGKLAYVWCTVQRAFSYDFPLCAVDIDTPDGRTIHADVATAVVCNGKYYGGPFIAAPLADIS
HPTFQVILLKSPGLRHVARYALALAMGRLPRLPDVEIIEATRVRIALQGAQPLQADGDTVAHMPVDIVMAAEPVSLIVPA
PAA

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=217, Percent_Identity=29.9539170506912, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33572; Mature: 33572

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLPFAPANPAPAPAQRRVMIIANPAAGSLKQRRLNRTLLGLEKLGCTVGLRETAKPGD
CEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCCC
ATLMAREAALAGNVDVVVAAGGDGTINEVANGLAGSGVALGVIPLGTANVLAIEAGIPRD
EEEEEEHHHHCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCC
PGKAAQVIATGVLRKLYLGEVRASAETPLAGPRRFVMMAGAGFDAHVVDTVDLALKRKTG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEHHHHEEECCCC
KLAYVWCTVQRAFSYDFPLCAVDIDTPDGRTIHADVATAVVCNGKYYGGPFIAAPLADIS
CEEEEEEEEEHHHCCCCCEEEEEECCCCCCEEEECEEEEEEECCEEECCCEEECCHHHCC
HPTFQVILLKSPGLRHVARYALALAMGRLPRLPDVEIIEATRVRIALQGAQPLQADGDTV
CCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCEE
AHMPVDIVMAAEPVSLIVPAPAA
EECCEEEEEECCCEEEEECCCCC
>Mature Secondary Structure
MRVLPFAPANPAPAPAQRRVMIIANPAAGSLKQRRLNRTLLGLEKLGCTVGLRETAKPGD
CEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEECCCCCCCCCC
ATLMAREAALAGNVDVVVAAGGDGTINEVANGLAGSGVALGVIPLGTANVLAIEAGIPRD
EEEEEEHHHHCCCEEEEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCEEEEEECCCCCC
PGKAAQVIATGVLRKLYLGEVRASAETPLAGPRRFVMMAGAGFDAHVVDTVDLALKRKTG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCEEEEHHHHEEECCCC
KLAYVWCTVQRAFSYDFPLCAVDIDTPDGRTIHADVATAVVCNGKYYGGPFIAAPLADIS
CEEEEEEEEEHHHCCCCCEEEEEECCCCCCEEEECEEEEEEECCEEECCCEEECCHHHCC
HPTFQVILLKSPGLRHVARYALALAMGRLPRLPDVEIIEATRVRIALQGAQPLQADGDTV
CCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCCCCCEE
AHMPVDIVMAAEPVSLIVPAPAA
EECCEEEEEECCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]