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The map label for this gene is rsmE [H]

Identifier: 83591910

GI number: 83591910

Start: 678246

End: 678989

Strand: Reverse

Name: rsmE [H]

Synonym: Rru_A0571

Alternate gene names: 83591910

Gene position: 678989-678246 (Counterclockwise)

Preceding gene: 83591916

Following gene: 83591909

Centisome position: 15.6

GC content: 71.64

Gene sequence:

>744_bases
ATGATTGCCCAACCCCCGCGCCACCGCCTGTATGTCGACGCCCCCCTGGCCGCCGGCCTTGATGTCCGCCTGGATGGCGC
CCAGGCCCACTACCTGCGCGATGTGATGCGCCTGGGCCCCGGGGCGGCTGTCGCCCTGTTCAACGGCCGTGATGGCGAAT
GGCTGGCGACCCTCGACACCCTGGGCAAGGCCGGCGCCCAGGCGGTGGCGCAAACCCTTTTGCGCCCCCAGGGCGACGAG
GACGGCCCGTGGCTGCTGTTCGCCCCGCTCAAGAAGGAAGCCACCGATTACCTTGTCGAAAAGGCGGTGGAACTGGGCTG
CGGAGCCTTGCTGCCGGTGATGACCCGGCGCACCCAGTCGCAGAAGGTGCGCACCGACCGCCTGCGCGCCCAGGTGATCG
GCGCCGCCGAGCAATGCGAGCGCCTGACTCTGCCCGCCGTCTTCGAGCCGCGCCCTTTGGCGAAGGTATTGGAGGACTGG
CCGGCCGAGCGCCGGTTGTTCGTGCTGGCCGAGCGCCGCGACGCCCTTCCCGCCGCCCGGGCTTTCGCGGGCGCCCGCGC
TCTAAAGGCCGCCCTTCTCGTCGGGCCGGAAGGTGGGCTTGATGACAAGGACCTTGACGCGCTGTTGGCCCTGCCCTCAT
CTACGCCCGTCGGCCTCGGCCCGCGCATCCTGCGCGCCGAGACGGCGGCGGCGGCGGCGCTCGCCGTCTGGCAGGCGGTC
GCAGGGGATTGGGAGACCCGCTAA

Upstream 100 bases:

>100_bases
TTGTCTGGCTAGAGCAGCTCTCAGCCCAGACTTGCCCCTTATAGCCGGTACAGTATGGTGTCCGCCACCCAGGAACAAGA
CCAAGAAAGCCGCTCGCCCC

Downstream 100 bases:

>100_bases
GGGGGCATGACCCCGCCGGCCTCCGCAGCCCGACCGATGCAAGACGGCCCGAGGGCCTCGCGCGCGAATGACCTCTGGAA
CGGAAGCCTGCCATGTCCTC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MIAQPPRHRLYVDAPLAAGLDVRLDGAQAHYLRDVMRLGPGAAVALFNGRDGEWLATLDTLGKAGAQAVAQTLLRPQGDE
DGPWLLFAPLKKEATDYLVEKAVELGCGALLPVMTRRTQSQKVRTDRLRAQVIGAAEQCERLTLPAVFEPRPLAKVLEDW
PAERRLFVLAERRDALPAARAFAGARALKAALLVGPEGGLDDKDLDALLALPSSTPVGLGPRILRAETAAAAALAVWQAV
AGDWETR

Sequences:

>Translated_247_residues
MIAQPPRHRLYVDAPLAAGLDVRLDGAQAHYLRDVMRLGPGAAVALFNGRDGEWLATLDTLGKAGAQAVAQTLLRPQGDE
DGPWLLFAPLKKEATDYLVEKAVELGCGALLPVMTRRTQSQKVRTDRLRAQVIGAAEQCERLTLPAVFEPRPLAKVLEDW
PAERRLFVLAERRDALPAARAFAGARALKAALLVGPEGGLDDKDLDALLALPSSTPVGLGPRILRAETAAAAALAVWQAV
AGDWETR
>Mature_247_residues
MIAQPPRHRLYVDAPLAAGLDVRLDGAQAHYLRDVMRLGPGAAVALFNGRDGEWLATLDTLGKAGAQAVAQTLLRPQGDE
DGPWLLFAPLKKEATDYLVEKAVELGCGALLPVMTRRTQSQKVRTDRLRAQVIGAAEQCERLTLPAVFEPRPLAKVLEDW
PAERRLFVLAERRDALPAARAFAGARALKAALLVGPEGGLDDKDLDALLALPSSTPVGLGPRILRAETAAAAALAVWQAV
AGDWETR

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=249, Percent_Identity=29.718875502008, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26515; Mature: 26515

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAQPPRHRLYVDAPLAAGLDVRLDGAQAHYLRDVMRLGPGAAVALFNGRDGEWLATLDT
CCCCCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHH
LGKAGAQAVAQTLLRPQGDEDGPWLLFAPLKKEATDYLVEKAVELGCGALLPVMTRRTQS
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QKVRTDRLRAQVIGAAEQCERLTLPAVFEPRPLAKVLEDWPAERRLFVLAERRDALPAAR
HHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHH
AFAGARALKAALLVGPEGGLDDKDLDALLALPSSTPVGLGPRILRAETAAAAALAVWQAV
HHHHHHHHHHHEEECCCCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGDWETR
HCCCCCC
>Mature Secondary Structure
MIAQPPRHRLYVDAPLAAGLDVRLDGAQAHYLRDVMRLGPGAAVALFNGRDGEWLATLDT
CCCCCCCCEEEECCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHH
LGKAGAQAVAQTLLRPQGDEDGPWLLFAPLKKEATDYLVEKAVELGCGALLPVMTRRTQS
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
QKVRTDRLRAQVIGAAEQCERLTLPAVFEPRPLAKVLEDWPAERRLFVLAERRDALPAAR
HHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHH
AFAGARALKAALLVGPEGGLDDKDLDALLALPSSTPVGLGPRILRAETAAAAALAVWQAV
HHHHHHHHHHHEEECCCCCCCCCCCHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGDWETR
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]