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The map label for this gene is pmbA [H]

Identifier: 83591914

GI number: 83591914

Start: 682439

End: 683797

Strand: Direct

Name: pmbA [H]

Synonym: Rru_A0575

Alternate gene names: 83591914

Gene position: 682439-683797 (Clockwise)

Preceding gene: 83591913

Following gene: 83591915

Centisome position: 15.68

GC content: 68.58

Gene sequence:

>1359_bases
ATGACCGATCTAGCCAAGACCGCCGCCAATGCCCTGGGCCTGCTCGAGGATCTGGTGGCCAAGGCGGTGGCCGCCGGAGC
GACGGCGGCCGACGCGGTGCTGGTCGATGGCGCCTCGCTGGCCGTCACCCATCGCTTGGGCAAGCTTGAGAAGCTGGAGC
GCTCGGAAGGCGGCGATATCGGATTGCGGGTGCTGATCGGCGGCCGTCAGGCCATCGTGTCGTCGGCCGATCGCCGCCCC
GATGCCCTGGCCGCCCTGGTCGAGCGCGCCGTGGCGATGGCGCGCACCGTGCCCGAAGATCCCTTCGCCGGCTTGGCCGA
GCCCGGCCAGCTTGCCCGCGACCTGCCCGCCATCGACAACTTCGATCCGACCGAACCCTCGGCCGAGGCGCTGACCGAAC
TGGTGCGCGCCCTGGAGGACGAGGCACGCAGCGTTCCCGGCGTGACGAATTCCGAAGGGGCCGAGGCCAGTTGGGGGCTG
TCAACCGTTGCCCTGGTCGGCTCCAACGGCTTCTCCCATGCCTATCACGCCAGCCATTCGGGCATGAGCGTTTCCGTGGT
CGCCGGAACCAACGAGACCGGCATGGAAGGCGATTACGATTATTCGACCGCCGTTTATTTCGCCGATCTGCGCGATCCGC
TCGAAGTCGGCCGCGAAGCCGGGCGGCGCGCCACCCGCCGGCTTGGCGCCAAACGGCTGGCGACCGGCCGCCTGCCGGTG
GTTTTCGATCCCCGGGTGTCGCGCGGGCTGGTCGGCCACTTGCTTGGCGCCATCAATGGCGGCGGCATCGCGCGCGGCAC
CTCGTTCCTTAAGGATCGTTTGGGCGAGGCGGTCTTCGCCCCGGGCATTCGCATCATCGAAGATCCCCACCGCCCGCGCG
GCCTGCGCTCCAAGCCCTGCGACGCCGAGGGGTTGGCCAATCGCCGCCGCACCCTGGTCGAAGATGGCCGATTGACCAGT
TGGATCCTCGATCTGCGCTCGGCCCGCCAGTTGGGTCTGGAAAGCACCGGCCACGCCAGTCGCGGCACCGGCTCGGCGCC
CAGCCCGTCGACCACCAATGTCTGGCTCGAAGCCGGACCGCTCAGCCCCCAGGACCTGATGGCCGATATCGAGGATGGTT
TGTATGTCACCGATCTGGTCGGCCAGGGGGTGAACGGCCTGACCGGTGATTACAGCCGGGGAGCGGCCGGCTTCCGCATC
GAAAAGGGCGTGCTGACCACGCCGGTCAACGAGATCACCATTGCCGGCAATCTGATCGAGATGTTCGCCGGGCTGACCCC
GGCCAATGACCTCGATTTGCGCTACGGCACCGACGCGCCGACCGTGCGTCTGGCGGCGATGACCATCGCCGGCGGCTGA

Upstream 100 bases:

>100_bases
CGATTGCCGCCGGCCAGCGCGCGGCCCACCTAACTAAGGGCACGGGCGCGACGTCCCGGCCGCGCCCGTGCGTCTTTCGT
TTCCCTTTCGGAGTGCTCCC

Downstream 100 bases:

>100_bases
GCCCGGCCGCCCCCGGCCGCTGCCGGGGGTGTCATAAAACCGCCAGCGACGGAGGGCGGAAGACCCGTTACAAGACTCCC
CAGGGACCACAAGGCCTGGG

Product: microcin-processing peptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MTDLAKTAANALGLLEDLVAKAVAAGATAADAVLVDGASLAVTHRLGKLEKLERSEGGDIGLRVLIGGRQAIVSSADRRP
DALAALVERAVAMARTVPEDPFAGLAEPGQLARDLPAIDNFDPTEPSAEALTELVRALEDEARSVPGVTNSEGAEASWGL
STVALVGSNGFSHAYHASHSGMSVSVVAGTNETGMEGDYDYSTAVYFADLRDPLEVGREAGRRATRRLGAKRLATGRLPV
VFDPRVSRGLVGHLLGAINGGGIARGTSFLKDRLGEAVFAPGIRIIEDPHRPRGLRSKPCDAEGLANRRRTLVEDGRLTS
WILDLRSARQLGLESTGHASRGTGSAPSPSTTNVWLEAGPLSPQDLMADIEDGLYVTDLVGQGVNGLTGDYSRGAAGFRI
EKGVLTTPVNEITIAGNLIEMFAGLTPANDLDLRYGTDAPTVRLAAMTIAGG

Sequences:

>Translated_452_residues
MTDLAKTAANALGLLEDLVAKAVAAGATAADAVLVDGASLAVTHRLGKLEKLERSEGGDIGLRVLIGGRQAIVSSADRRP
DALAALVERAVAMARTVPEDPFAGLAEPGQLARDLPAIDNFDPTEPSAEALTELVRALEDEARSVPGVTNSEGAEASWGL
STVALVGSNGFSHAYHASHSGMSVSVVAGTNETGMEGDYDYSTAVYFADLRDPLEVGREAGRRATRRLGAKRLATGRLPV
VFDPRVSRGLVGHLLGAINGGGIARGTSFLKDRLGEAVFAPGIRIIEDPHRPRGLRSKPCDAEGLANRRRTLVEDGRLTS
WILDLRSARQLGLESTGHASRGTGSAPSPSTTNVWLEAGPLSPQDLMADIEDGLYVTDLVGQGVNGLTGDYSRGAAGFRI
EKGVLTTPVNEITIAGNLIEMFAGLTPANDLDLRYGTDAPTVRLAAMTIAGG
>Mature_451_residues
TDLAKTAANALGLLEDLVAKAVAAGATAADAVLVDGASLAVTHRLGKLEKLERSEGGDIGLRVLIGGRQAIVSSADRRPD
ALAALVERAVAMARTVPEDPFAGLAEPGQLARDLPAIDNFDPTEPSAEALTELVRALEDEARSVPGVTNSEGAEASWGLS
TVALVGSNGFSHAYHASHSGMSVSVVAGTNETGMEGDYDYSTAVYFADLRDPLEVGREAGRRATRRLGAKRLATGRLPVV
FDPRVSRGLVGHLLGAINGGGIARGTSFLKDRLGEAVFAPGIRIIEDPHRPRGLRSKPCDAEGLANRRRTLVEDGRLTSW
ILDLRSARQLGLESTGHASRGTGSAPSPSTTNVWLEAGPLSPQDLMADIEDGLYVTDLVGQGVNGLTGDYSRGAAGFRIE
KGVLTTPVNEITIAGNLIEMFAGLTPANDLDLRYGTDAPTVRLAAMTIAGG

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=444, Percent_Identity=38.2882882882883, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789640, Length=256, Percent_Identity=27.734375, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 47126; Mature: 46994

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLAKTAANALGLLEDLVAKAVAAGATAADAVLVDGASLAVTHRLGKLEKLERSEGGDI
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEECCCHHHHHHHHHHHHHHHHCCCCCE
GLRVLIGGRQAIVSSADRRPDALAALVERAVAMARTVPEDPFAGLAEPGQLARDLPAIDN
EEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCC
FDPTEPSAEALTELVRALEDEARSVPGVTNSEGAEASWGLSTVALVGSNGFSHAYHASHS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCCC
GMSVSVVAGTNETGMEGDYDYSTAVYFADLRDPLEVGREAGRRATRRLGAKRLATGRLPV
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VFDPRVSRGLVGHLLGAINGGGIARGTSFLKDRLGEAVFAPGIRIIEDPHRPRGLRSKPC
EECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCCEECCCCCCCCCCCCCC
DAEGLANRRRTLVEDGRLTSWILDLRSARQLGLESTGHASRGTGSAPSPSTTNVWLEAGP
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECC
LSPQDLMADIEDGLYVTDLVGQGVNGLTGDYSRGAAGFRIEKGVLTTPVNEITIAGNLIE
CCHHHHHHHHHCCEEEEHHHCCCCCCCCCCCCCCCCCCEEECCCEECCCCCEEEHHHHHH
MFAGLTPANDLDLRYGTDAPTVRLAAMTIAGG
HHHCCCCCCCCCEEECCCCCCEEEEEEEECCC
>Mature Secondary Structure 
TDLAKTAANALGLLEDLVAKAVAAGATAADAVLVDGASLAVTHRLGKLEKLERSEGGDI
CHHHHHHHHHHHHHHHHHHHHHHCCCCHHCEEEECCCHHHHHHHHHHHHHHHHCCCCCE
GLRVLIGGRQAIVSSADRRPDALAALVERAVAMARTVPEDPFAGLAEPGQLARDLPAIDN
EEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCC
FDPTEPSAEALTELVRALEDEARSVPGVTNSEGAEASWGLSTVALVGSNGFSHAYHASHS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHCCCCC
GMSVSVVAGTNETGMEGDYDYSTAVYFADLRDPLEVGREAGRRATRRLGAKRLATGRLPV
CCEEEEEECCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VFDPRVSRGLVGHLLGAINGGGIARGTSFLKDRLGEAVFAPGIRIIEDPHRPRGLRSKPC
EECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHCCCCCEECCCCCCCCCCCCCC
DAEGLANRRRTLVEDGRLTSWILDLRSARQLGLESTGHASRGTGSAPSPSTTNVWLEAGP
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECC
LSPQDLMADIEDGLYVTDLVGQGVNGLTGDYSRGAAGFRIEKGVLTTPVNEITIAGNLIE
CCHHHHHHHHHCCEEEEHHHCCCCCCCCCCCCCCCCCCEEECCCEECCCCCEEEHHHHHH
MFAGLTPANDLDLRYGTDAPTVRLAAMTIAGG
HHHCCCCCCCCCEEECCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]