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The map label for this gene is 83591909

Identifier: 83591909

GI number: 83591909

Start: 676783

End: 678153

Strand: Reverse

Name: 83591909

Synonym: Rru_A0570

Alternate gene names: NA

Gene position: 678153-676783 (Counterclockwise)

Preceding gene: 83591910

Following gene: 83591908

Centisome position: 15.58

GC content: 65.5

Gene sequence:

>1371_bases
ATGTCCTCCTTGACCGATGCCTCGATCCTTTCGGCGCCTTTGACCCGGGACGATCTGGTCGCCCATATGGCGGGCGGCAG
CCGCCCAAAGGCCGATTGGCGCATCGGCACCGAACACGAGAAATACGCCTTCACCATCGCCGATGGCCGGCCCCTGACCT
ATGAGGGACCCCATGGCGTGCGCGCCCTGCTCGAGGGACTGACCCGCTTCGGCTGGAGCCCGGTCTATGAGGGCGAGACG
GTGATCGCGCTGACCTGCCCGACCATGGGCTCGGTGACCCTGGAGCCCGGCGGTCAGATCGAGCTCTCGGGAGCGCAGCT
GGAAACCGTGCATGGCACCTGCTGCGAGGTGACGACCCACCACCGCAACCTCAAGATCGTCGGCGGCGAGTTGGGCATCG
GCTTCCTGGGTATCGGCTTCAATCCGAAATGGCGGCGCGAGGATATCTCGCTGATGCCCAAGGGCCGCTACAAGCTGATG
TCGGAGTACATGCCCAAGGTCGGCTCGCTCGGCCTCGACATGATGCTGCGCACCTGCACGGTGCAAACCAATCTGGACTT
CGGCAGCGAGGCCGACATGGTGCGGAAGTTCCGCGTCTCGCTCGCCCTTCAGCCGATCGCCACGGCGCTGTGGGCCAACA
GCCCCTTCACCGAGGGCAAGCCCAACGGCTTTCTGTCCTATCGCAGCCATATCTGGACCGATACGGACCCGGCGCGCACC
GGTATCTTGCCCTTCGTCTTCGAAGACGGCATGGGCTTCGAGCGGTATGTCGATTATCTGCTCGATGTGCCGATGTACTT
CGTCTACCGCGAGGGCCGCTATATCGACGCCACCGGCCAGTCGTTCCGCGATTTCATGGCCGGCCGCCTGGCCGCCCTGC
CCGGCGAGCGGCCGACGATCGCCGATTGGACCGACCATATGACCGTCGCCTTCCCCGAGGTGCGCCTCAAGCGCTATCTG
GAAATGCGCGGCGCCGATGGCGGGCCGTGGCTGCGCCTGTGCGCCCTGCCCGCGTTCTGGGTCGGCCTGCTCTATGAAAC
CGCCTCGCTTGATGCCGCCTGGGATCTGGTCAAGGATTGGACGGCCGAGGAACACGCCCAATTGCGCGCCCAGGTGCCGC
GACTGGCCCTCAAAACGCCGTTCCGCAAGGGCACCTTGCAAGACGTCGCCCTGCGCGCCCTGGAGATTTCCCAGGCCGGA
CTGGCGGCGCGCGGTCGCCTGAACAGCAAGGGCCGCGACGAGACCGGCTTCCTTGATCCCTTGTGGACCATCGCGCGCAG
CGGCCGCACCCAGGCCGAAGACCTGCTCGAGTCCTTCCTGACCCGATGGAATGGCGAGATCGACCCCGTTTTCACCGAAT
GCGCCTATTAA

Upstream 100 bases:

>100_bases
CCCGCTAAGGGGGCATGACCCCGCCGGCCTCCGCAGCCCGACCGATGCAAGACGGCCCGAGGGCCTCGCGCGCGAATGAC
CTCTGGAACGGAAGCCTGCC

Downstream 100 bases:

>100_bases
GTCAGGCGCTGGCCGCGGCCTTACCGTCCGCCCGTCCGTAACCAGCCGGAGTTTTTCCCGTGACCCGACCCAACACCGTT
TCCTCCTATCCCGCGGCCAA

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSSLTDASILSAPLTRDDLVAHMAGGSRPKADWRIGTEHEKYAFTIADGRPLTYEGPHGVRALLEGLTRFGWSPVYEGET
VIALTCPTMGSVTLEPGGQIELSGAQLETVHGTCCEVTTHHRNLKIVGGELGIGFLGIGFNPKWRREDISLMPKGRYKLM
SEYMPKVGSLGLDMMLRTCTVQTNLDFGSEADMVRKFRVSLALQPIATALWANSPFTEGKPNGFLSYRSHIWTDTDPART
GILPFVFEDGMGFERYVDYLLDVPMYFVYREGRYIDATGQSFRDFMAGRLAALPGERPTIADWTDHMTVAFPEVRLKRYL
EMRGADGGPWLRLCALPAFWVGLLYETASLDAAWDLVKDWTAEEHAQLRAQVPRLALKTPFRKGTLQDVALRALEISQAG
LAARGRLNSKGRDETGFLDPLWTIARSGRTQAEDLLESFLTRWNGEIDPVFTECAY

Sequences:

>Translated_456_residues
MSSLTDASILSAPLTRDDLVAHMAGGSRPKADWRIGTEHEKYAFTIADGRPLTYEGPHGVRALLEGLTRFGWSPVYEGET
VIALTCPTMGSVTLEPGGQIELSGAQLETVHGTCCEVTTHHRNLKIVGGELGIGFLGIGFNPKWRREDISLMPKGRYKLM
SEYMPKVGSLGLDMMLRTCTVQTNLDFGSEADMVRKFRVSLALQPIATALWANSPFTEGKPNGFLSYRSHIWTDTDPART
GILPFVFEDGMGFERYVDYLLDVPMYFVYREGRYIDATGQSFRDFMAGRLAALPGERPTIADWTDHMTVAFPEVRLKRYL
EMRGADGGPWLRLCALPAFWVGLLYETASLDAAWDLVKDWTAEEHAQLRAQVPRLALKTPFRKGTLQDVALRALEISQAG
LAARGRLNSKGRDETGFLDPLWTIARSGRTQAEDLLESFLTRWNGEIDPVFTECAY
>Mature_455_residues
SSLTDASILSAPLTRDDLVAHMAGGSRPKADWRIGTEHEKYAFTIADGRPLTYEGPHGVRALLEGLTRFGWSPVYEGETV
IALTCPTMGSVTLEPGGQIELSGAQLETVHGTCCEVTTHHRNLKIVGGELGIGFLGIGFNPKWRREDISLMPKGRYKLMS
EYMPKVGSLGLDMMLRTCTVQTNLDFGSEADMVRKFRVSLALQPIATALWANSPFTEGKPNGFLSYRSHIWTDTDPARTG
ILPFVFEDGMGFERYVDYLLDVPMYFVYREGRYIDATGQSFRDFMAGRLAALPGERPTIADWTDHMTVAFPEVRLKRYLE
MRGADGGPWLRLCALPAFWVGLLYETASLDAAWDLVKDWTAEEHAQLRAQVPRLALKTPFRKGTLQDVALRALEISQAGL
AARGRLNSKGRDETGFLDPLWTIARSGRTQAEDLLESFLTRWNGEIDPVFTECAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 50817; Mature: 50685

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLTDASILSAPLTRDDLVAHMAGGSRPKADWRIGTEHEKYAFTIADGRPLTYEGPHGV
CCCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCEECCCCCEEEEEEECCCEEEECCCHHH
RALLEGLTRFGWSPVYEGETVIALTCPTMGSVTLEPGGQIELSGAQLETVHGTCCEVTTH
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCCEEEECCCEEEECCCCEEEEEEC
HRNLKIVGGELGIGFLGIGFNPKWRREDISLMPKGRYKLMSEYMPKVGSLGLDMMLRTCT
CCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCHHCCCCHHHHHHHHE
VQTNLDFGSEADMVRKFRVSLALQPIATALWANSPFTEGKPNGFLSYRSHIWTDTDPART
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHCCCCCCCCHHH
GILPFVFEDGMGFERYVDYLLDVPMYFVYREGRYIDATGQSFRDFMAGRLAALPGERPTI
CCCEEEEECCCCHHHHHHHHHHCCEEEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCC
ADWTDHMTVAFPEVRLKRYLEMRGADGGPWLRLCALPAFWVGLLYETASLDAAWDLVKDW
CCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
TAEEHAQLRAQVPRLALKTPFRKGTLQDVALRALEISQAGLAARGRLNSKGRDETGFLDP
CHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCH
LWTIARSGRTQAEDLLESFLTRWNGEIDPVFTECAY
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure 
SSLTDASILSAPLTRDDLVAHMAGGSRPKADWRIGTEHEKYAFTIADGRPLTYEGPHGV
CCCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCEECCCCCEEEEEEECCCEEEECCCHHH
RALLEGLTRFGWSPVYEGETVIALTCPTMGSVTLEPGGQIELSGAQLETVHGTCCEVTTH
HHHHHHHHHCCCCCCCCCCEEEEEECCCCCCEEECCCCEEEECCCEEEECCCCEEEEEEC
HRNLKIVGGELGIGFLGIGFNPKWRREDISLMPKGRYKLMSEYMPKVGSLGLDMMLRTCT
CCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCHHCCCCHHHHHHHHE
VQTNLDFGSEADMVRKFRVSLALQPIATALWANSPFTEGKPNGFLSYRSHIWTDTDPART
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHCCCCCCCCHHH
GILPFVFEDGMGFERYVDYLLDVPMYFVYREGRYIDATGQSFRDFMAGRLAALPGERPTI
CCCEEEEECCCCHHHHHHHHHHCCEEEEEECCEEEECCCHHHHHHHHHHHHCCCCCCCCC
ADWTDHMTVAFPEVRLKRYLEMRGADGGPWLRLCALPAFWVGLLYETASLDAAWDLVKDW
CCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
TAEEHAQLRAQVPRLALKTPFRKGTLQDVALRALEISQAGLAARGRLNSKGRDETGFLDP
CHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCH
LWTIARSGRTQAEDLLESFLTRWNGEIDPVFTECAY
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA