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The map label for this gene is amiC [H]

Identifier: 83591902

GI number: 83591902

Start: 665587

End: 666852

Strand: Reverse

Name: amiC [H]

Synonym: Rru_A0563

Alternate gene names: 83591902

Gene position: 666852-665587 (Counterclockwise)

Preceding gene: 83591904

Following gene: 83591901

Centisome position: 15.32

GC content: 67.77

Gene sequence:

>1266_bases
ATGAACAAGCAGCCAGCCGCGCGGACGGCTTTGAAAAAACCGGCCTCCGGGGTGGGGCGACGGGCCTTTCTCGCCGGATC
GGCCGCCGGCATTCTGGGGCTGGCCGCGCCGGCGATCCGCCGGGCCCAGGCCGCCGAGGCGATTCGCATCGGCGAGATCA
ACAGCTACAGCCAGATTCCGGCCTTCACCCTGCCCTATCGCAACGGCTGGCAACTGGCGGTGGAGCAGATCAACGCCGCC
GGCGGGTTGCTGGGCGGCCGGCCGCTGGAGGTCATCAGCCGCGACGACGGCGGCGATCCGGGCAAGGCGGTGACCGCCGC
CCAGGAGTTGCTGACCCGCCACGGCGTCCACGCCCTGGCCGGCACCTTCCTGTCCCATGTCGGTCTGGCGGTGTCGGATT
TCGCCCGCCAGCGCAAGGTGTTGTTCATGGCCTCCGAGCCCTTGACCGACGCCCTGACCTGGGAAAAGGGCAACCGCTAT
ACCTATCGCCTGCGGCCGAGCACCTATATGCAGGCGGCGATGCTGGCGGCCGAAGCGGCGAAGCTACCGATCACCCGGTG
GGCGACCATCGCCCCCAATTACGAATATGGCCAGTCGGCGGTGGCCCGCTTCAAGGAACTGCTGCTGGCCGCCCGCCCCG
AGGTGACCTTCGTCGCCGAGCAATGGCCCGCCCTTTACAAGCTTGACGCCGGCCCCACCGTTCAGGCCCTGCAGCAGGCC
GAACCCGAGGGCCTCTTCAATGTGCTGTTTGGCGCCGATCTGCCGAAATTCGTGCGCGAGGGACGGGTGCGCGGGTTGTT
CGCCGGCCGTCAGGTGGTCAGCATGCTGACCGGCGAGCCGGAATATCTCAACCCGCTCAAGGACGAGGCGCCCGAGGGCT
GGATCGTCACCGGTTATCCCTGGTACGATATCGACACCGCGCCGCACCGCGCCTTCGTCGAGGCCTATCGCGCCCGCTGG
AAGGAAGATCCCTTCGTCGGCTCGCTGGTGGGCTATAACACCCTAACGGCGATGGCCGTCGCCTTTGAAAAGGCCGGCGG
CACCGAAAGTGAAACCCTGGTCGAGACCCTTAAGGACATGGCGTTTTCCACCCCGATGGGGCCGCTTTCCTTCCGCGCCA
GCGATCACCAGTCGACCATGGGCGCCTGGGTCGGCCGCACGGCCCTGCGCGATGGCAAAGGGGTGATGGTCGATTGGCGC
TATGTGGATGGCGGCTCCGTTCTGCCGCCGCCCGAAGTGGTCAGCGCGTGGCGCCCCGCCGGCTGA

Upstream 100 bases:

>100_bases
AATCTTCTTTTGCGCGCGACCACCCCGCCAGCCTTGACAGTCCCCAACCAAGTGGAAATAGTCATTTTGAGCATTTCTTG
ATGGGCGGGGAAAACCGATC

Downstream 100 bases:

>100_bases
TCGCCCCCCTGGTCCGGCCGCTGCTTCTCGCCCTGGCTTTTGGCCTTATCGGCGGAGCGGCGGCGGCCCAATCGGCGCGC
GATCTGCCCGCCGAAAGCGA

Product: twin-arginine translocation pathway signal

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNKQPAARTALKKPASGVGRRAFLAGSAAGILGLAAPAIRRAQAAEAIRIGEINSYSQIPAFTLPYRNGWQLAVEQINAA
GGLLGGRPLEVISRDDGGDPGKAVTAAQELLTRHGVHALAGTFLSHVGLAVSDFARQRKVLFMASEPLTDALTWEKGNRY
TYRLRPSTYMQAAMLAAEAAKLPITRWATIAPNYEYGQSAVARFKELLLAARPEVTFVAEQWPALYKLDAGPTVQALQQA
EPEGLFNVLFGADLPKFVREGRVRGLFAGRQVVSMLTGEPEYLNPLKDEAPEGWIVTGYPWYDIDTAPHRAFVEAYRARW
KEDPFVGSLVGYNTLTAMAVAFEKAGGTESETLVETLKDMAFSTPMGPLSFRASDHQSTMGAWVGRTALRDGKGVMVDWR
YVDGGSVLPPPEVVSAWRPAG

Sequences:

>Translated_421_residues
MNKQPAARTALKKPASGVGRRAFLAGSAAGILGLAAPAIRRAQAAEAIRIGEINSYSQIPAFTLPYRNGWQLAVEQINAA
GGLLGGRPLEVISRDDGGDPGKAVTAAQELLTRHGVHALAGTFLSHVGLAVSDFARQRKVLFMASEPLTDALTWEKGNRY
TYRLRPSTYMQAAMLAAEAAKLPITRWATIAPNYEYGQSAVARFKELLLAARPEVTFVAEQWPALYKLDAGPTVQALQQA
EPEGLFNVLFGADLPKFVREGRVRGLFAGRQVVSMLTGEPEYLNPLKDEAPEGWIVTGYPWYDIDTAPHRAFVEAYRARW
KEDPFVGSLVGYNTLTAMAVAFEKAGGTESETLVETLKDMAFSTPMGPLSFRASDHQSTMGAWVGRTALRDGKGVMVDWR
YVDGGSVLPPPEVVSAWRPAG
>Mature_421_residues
MNKQPAARTALKKPASGVGRRAFLAGSAAGILGLAAPAIRRAQAAEAIRIGEINSYSQIPAFTLPYRNGWQLAVEQINAA
GGLLGGRPLEVISRDDGGDPGKAVTAAQELLTRHGVHALAGTFLSHVGLAVSDFARQRKVLFMASEPLTDALTWEKGNRY
TYRLRPSTYMQAAMLAAEAAKLPITRWATIAPNYEYGQSAVARFKELLLAARPEVTFVAEQWPALYKLDAGPTVQALQQA
EPEGLFNVLFGADLPKFVREGRVRGLFAGRQVVSMLTGEPEYLNPLKDEAPEGWIVTGYPWYDIDTAPHRAFVEAYRARW
KEDPFVGSLVGYNTLTAMAVAFEKAGGTESETLVETLKDMAFSTPMGPLSFRASDHQSTMGAWVGRTALRDGKGVMVDWR
YVDGGSVLPPPEVVSAWRPAG

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45621; Mature: 45621

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQPAARTALKKPASGVGRRAFLAGSAAGILGLAAPAIRRAQAAEAIRIGEINSYSQIP
CCCCCHHHHHHHCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
AFTLPYRNGWQLAVEQINAAGGLLGGRPLEVISRDDGGDPGKAVTAAQELLTRHGVHALA
EEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHH
GTFLSHVGLAVSDFARQRKVLFMASEPLTDALTWEKGNRYTYRLRPSTYMQAAMLAAEAA
HHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
KLPITRWATIAPNYEYGQSAVARFKELLLAARPEVTFVAEQWPALYKLDAGPTVQALQQA
CCCCHHEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHC
EPEGLFNVLFGADLPKFVREGRVRGLFAGRQVVSMLTGEPEYLNPLKDEAPEGWIVTGYP
CCCHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCHHHCCCCCEEEECCC
WYDIDTAPHRAFVEAYRARWKEDPFVGSLVGYNTLTAMAVAFEKAGGTESETLVETLKDM
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AFSTPMGPLSFRASDHQSTMGAWVGRTALRDGKGVMVDWRYVDGGSVLPPPEVVSAWRPA
HHCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHCCCCC
G
C
>Mature Secondary Structure
MNKQPAARTALKKPASGVGRRAFLAGSAAGILGLAAPAIRRAQAAEAIRIGEINSYSQIP
CCCCCHHHHHHHCCCCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCC
AFTLPYRNGWQLAVEQINAAGGLLGGRPLEVISRDDGGDPGKAVTAAQELLTRHGVHALA
EEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHH
GTFLSHVGLAVSDFARQRKVLFMASEPLTDALTWEKGNRYTYRLRPSTYMQAAMLAAEAA
HHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHH
KLPITRWATIAPNYEYGQSAVARFKELLLAARPEVTFVAEQWPALYKLDAGPTVQALQQA
CCCCHHEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEECCCCHHHHHHHC
EPEGLFNVLFGADLPKFVREGRVRGLFAGRQVVSMLTGEPEYLNPLKDEAPEGWIVTGYP
CCCHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCHHHCCCCCEEEECCC
WYDIDTAPHRAFVEAYRARWKEDPFVGSLVGYNTLTAMAVAFEKAGGTESETLVETLKDM
CCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AFSTPMGPLSFRASDHQSTMGAWVGRTALRDGKGVMVDWRYVDGGSVLPPPEVVSAWRPA
HHCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]