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The map label for this gene is glgX [H]

Identifier: 83591844

GI number: 83591844

Start: 595288

End: 597432

Strand: Direct

Name: glgX [H]

Synonym: Rru_A0505

Alternate gene names: 83591844

Gene position: 595288-597432 (Clockwise)

Preceding gene: 83591843

Following gene: 83591845

Centisome position: 13.68

GC content: 63.64

Gene sequence:

>2145_bases
ATGAGCGCGCCGTCGACGCCCCTGGTTTGGCCGGGTCAGCCCTTTCCCCTGGGTGCGACCTGGGATGGATCGGGGACCAA
CTTCGCCTTGTTCTCGGCCCATGCCGAAAAGGTCGAGCTCTGTCTGTTCGACCGGGCGGGCCGCAAGGAAACCGCCCGCA
TCGTTCTGCCCGAATATACCGACGAGATTTGGCACGGTTATCTGCCCGACATCCGCCCCGGCCAGATGTACGGCTATCGG
GTTTATGGACCCTATGATCCCAATGCCGGCCACCGCTTCAACGCCAATAAGCTGCTGATCGACCCCTACGCCAAATCGCT
CAAGGGCGAGATCCTGTGGCATGACGCGCTGTTCGCCTACAAGCTCGGCCATCCCGATGGCGATCTGACCTTCGATCGCC
GCGACTCGGCCCGCCATATGCCCAAATGCGAGGTGGTCGATCCGGCCTTTACCTGGGGGCGGTCGATGGAGCCGCATCCC
TGGCATGAGACGGTGATCTACGAAATGCATCCGCGCGGCTTCACCATGCTCCACCCGGGGGTGCCCGAAGAGCTGCGCGG
CACCTTCGACGGCCTCGCCCACCGTCGCGTCACCGATTACCTGACCGATCTGGGGGTGACCGCCATCGAACTATTGCCCG
TCCATGCCTATGTGCAAGATCGCCACCTGATCGATCGCGGGCTGACCAATTACTGGGGCTATAATTCGATCGGTTTCCTC
GCCCCCCATGCCACCTATCTTGGCCATGGCCGCACCGCCTCCGCCTTCAAGGCCTTCGTCCAGAAGATGCACGACGCCGG
GCTCGAGGTGATCCTTGATGTGGTCTACAACCACACCGCCGAGGGCAATCATCTGGGGCCGACCCTGTCGTTCAAGGGCA
TCGACAACCGCTCGTATTATTATCTGATGGGCGAAGACCTCCGCCATTACAACAACTTCACCGGCACCGGCAACGCCTTG
GAACTGCGCCACCCGCGCGTTCTGGGCATGGTGATGGACTCGCTGCGCTATTGGGCGACGGCGATGGGGGTCGATGGTTT
TCGCTTCGATCTGGCGACCACCCTGGCCCGGGTCGAAGGGCCCTTCACCGAACACGCCGGTTTTCTGGACGCCGTGGCCC
AGGACCCCGTGCTCAGCCACGTCAAGCTGATCGCCGAGCCCTGGGATACGGGCCTGGGCGGCTATCAGGTCGGCGCCTTT
CCGCCGGGGTGGGCGGAATGGAACGACCAGTATCGCAATACCATTCGCAAGTTTTGGAAGGGCGATGACGGCCTGTTGCC
GACGATGGCCGGGCGGTTTTCGGCCTCCTCGGATGTCTTCAACCGCCGGGGCCGTCGGCCTTGGGCCAGCGTCAATTTCA
TCACCGCCCACGACGGCTTCACCCTGGCCGATCTGGTTTCCTACAACGGCAAGCACAACGAGGCCAACGGCGAGGACAAT
CGCGACGGCTCGGACGACAACAACTCGTGGAACTGCGGCGCCGAGGGGCCGACCGACGACGAGGAGATCAATACCCTGCG
CCGCCGCCAGATGCGCAACATGCTGGCCACCCTTTTGCTCTCCCAGGGCACGCCGATGCTGCTGGCCGGCGACGAATTCG
CCAATAGCCAGAATGGCAACAACAACGCCTATTGCCAGGACAACGCCCTAAGCTGGCTGGACTGGGACGGCATCGACGAA
AAGGCGCGCTCGCAGATCGCCTTCGTGACCAGCCTGCTGCGCCTGCGCCGCGAACATGTGGTCTTCCACCGCACCCGCTT
CTTTACCGGCTCGGTGATTCCGGGAACCGAGGTCAAGGATGTCGTCTGGTTGCGCCCGGACGGCGAGGAGATGACCGAGG
CCGATTGGGGCAATTCGGTCAGTCAGGCCCTGGCCATCCGTCTTTCGGGCGAAGCCGGCCTGACCCACCTGGATCGCCGG
GGCCGTCAGCAAACCGACGATACCTTCCTGCTGGTGCTCAACGCCAGCCACACCGAGGTCGTCTTCACCCTGCCCGAAGG
CGGACGTTGGGAAACCGTTATCGACACCATGGACGAGGATGGCCAGTCGAGTCTGCCCAAAGCGGATGGCGGGACCATCC
GCGCCCTGCCCGGGCGCAGCCTTCAGGTGCTGCGCTGCCGCGCCGCCCATGGGAGATCGGCATGA

Upstream 100 bases:

>100_bases
CAAACGCGTTGGTGGCGCGCAAACAATCCGAGGGGGCCACCGTGACGAATTTTCCACAAAGCATCGCCTTGTGTGTCCCT
GGCCGTCGCCGAGGTGCCCG

Downstream 100 bases:

>100_bases
CAAGACGGTTTGGCGCCCGGGTCGAGGGCGATGGCGTGCGGTTTTCGCTCTGGGCCCCCGCCGCCGAACGGGTTGAGGTG
GTTATCGAAGGCGCGGCCGA

Product: glycogen debranching protein GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 714; Mature: 713

Protein sequence:

>714_residues
MSAPSTPLVWPGQPFPLGATWDGSGTNFALFSAHAEKVELCLFDRAGRKETARIVLPEYTDEIWHGYLPDIRPGQMYGYR
VYGPYDPNAGHRFNANKLLIDPYAKSLKGEILWHDALFAYKLGHPDGDLTFDRRDSARHMPKCEVVDPAFTWGRSMEPHP
WHETVIYEMHPRGFTMLHPGVPEELRGTFDGLAHRRVTDYLTDLGVTAIELLPVHAYVQDRHLIDRGLTNYWGYNSIGFL
APHATYLGHGRTASAFKAFVQKMHDAGLEVILDVVYNHTAEGNHLGPTLSFKGIDNRSYYYLMGEDLRHYNNFTGTGNAL
ELRHPRVLGMVMDSLRYWATAMGVDGFRFDLATTLARVEGPFTEHAGFLDAVAQDPVLSHVKLIAEPWDTGLGGYQVGAF
PPGWAEWNDQYRNTIRKFWKGDDGLLPTMAGRFSASSDVFNRRGRRPWASVNFITAHDGFTLADLVSYNGKHNEANGEDN
RDGSDDNNSWNCGAEGPTDDEEINTLRRRQMRNMLATLLLSQGTPMLLAGDEFANSQNGNNNAYCQDNALSWLDWDGIDE
KARSQIAFVTSLLRLRREHVVFHRTRFFTGSVIPGTEVKDVVWLRPDGEEMTEADWGNSVSQALAIRLSGEAGLTHLDRR
GRQQTDDTFLLVLNASHTEVVFTLPEGGRWETVIDTMDEDGQSSLPKADGGTIRALPGRSLQVLRCRAAHGRSA

Sequences:

>Translated_714_residues
MSAPSTPLVWPGQPFPLGATWDGSGTNFALFSAHAEKVELCLFDRAGRKETARIVLPEYTDEIWHGYLPDIRPGQMYGYR
VYGPYDPNAGHRFNANKLLIDPYAKSLKGEILWHDALFAYKLGHPDGDLTFDRRDSARHMPKCEVVDPAFTWGRSMEPHP
WHETVIYEMHPRGFTMLHPGVPEELRGTFDGLAHRRVTDYLTDLGVTAIELLPVHAYVQDRHLIDRGLTNYWGYNSIGFL
APHATYLGHGRTASAFKAFVQKMHDAGLEVILDVVYNHTAEGNHLGPTLSFKGIDNRSYYYLMGEDLRHYNNFTGTGNAL
ELRHPRVLGMVMDSLRYWATAMGVDGFRFDLATTLARVEGPFTEHAGFLDAVAQDPVLSHVKLIAEPWDTGLGGYQVGAF
PPGWAEWNDQYRNTIRKFWKGDDGLLPTMAGRFSASSDVFNRRGRRPWASVNFITAHDGFTLADLVSYNGKHNEANGEDN
RDGSDDNNSWNCGAEGPTDDEEINTLRRRQMRNMLATLLLSQGTPMLLAGDEFANSQNGNNNAYCQDNALSWLDWDGIDE
KARSQIAFVTSLLRLRREHVVFHRTRFFTGSVIPGTEVKDVVWLRPDGEEMTEADWGNSVSQALAIRLSGEAGLTHLDRR
GRQQTDDTFLLVLNASHTEVVFTLPEGGRWETVIDTMDEDGQSSLPKADGGTIRALPGRSLQVLRCRAAHGRSA
>Mature_713_residues
SAPSTPLVWPGQPFPLGATWDGSGTNFALFSAHAEKVELCLFDRAGRKETARIVLPEYTDEIWHGYLPDIRPGQMYGYRV
YGPYDPNAGHRFNANKLLIDPYAKSLKGEILWHDALFAYKLGHPDGDLTFDRRDSARHMPKCEVVDPAFTWGRSMEPHPW
HETVIYEMHPRGFTMLHPGVPEELRGTFDGLAHRRVTDYLTDLGVTAIELLPVHAYVQDRHLIDRGLTNYWGYNSIGFLA
PHATYLGHGRTASAFKAFVQKMHDAGLEVILDVVYNHTAEGNHLGPTLSFKGIDNRSYYYLMGEDLRHYNNFTGTGNALE
LRHPRVLGMVMDSLRYWATAMGVDGFRFDLATTLARVEGPFTEHAGFLDAVAQDPVLSHVKLIAEPWDTGLGGYQVGAFP
PGWAEWNDQYRNTIRKFWKGDDGLLPTMAGRFSASSDVFNRRGRRPWASVNFITAHDGFTLADLVSYNGKHNEANGEDNR
DGSDDNNSWNCGAEGPTDDEEINTLRRRQMRNMLATLLLSQGTPMLLAGDEFANSQNGNNNAYCQDNALSWLDWDGIDEK
ARSQIAFVTSLLRLRREHVVFHRTRFFTGSVIPGTEVKDVVWLRPDGEEMTEADWGNSVSQALAIRLSGEAGLTHLDRRG
RQQTDDTFLLVLNASHTEVVFTLPEGGRWETVIDTMDEDGQSSLPKADGGTIRALPGRSLQVLRCRAAHGRSA

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=671, Percent_Identity=46.795827123696, Blast_Score=563, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80025; Mature: 79894

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSTPLVWPGQPFPLGATWDGSGTNFALFSAHAEKVELCLFDRAGRKETARIVLPEYT
CCCCCCCEECCCCCCCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEECCCHH
DEIWHGYLPDIRPGQMYGYRVYGPYDPNAGHRFNANKLLIDPYAKSLKGEILWHDALFAY
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHCCCCCEEEECCEEEE
KLGHPDGDLTFDRRDSARHMPKCEVVDPAFTWGRSMEPHPWHETVIYEMHPRGFTMLHPG
ECCCCCCCEEECCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCEEEEEECCCCEEEECCC
VPEELRGTFDGLAHRRVTDYLTDLGVTAIELLPVHAYVQDRHLIDRGLTNYWGYNSIGFL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCHHHCCCCCCCCC
APHATYLGHGRTASAFKAFVQKMHDAGLEVILDVVYNHTAEGNHLGPTLSFKGIDNRSYY
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEE
YLMGEDLRHYNNFTGTGNALELRHPRVLGMVMDSLRYWATAMGVDGFRFDLATTLARVEG
EEECCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PFTEHAGFLDAVAQDPVLSHVKLIAEPWDTGLGGYQVGAFPPGWAEWNDQYRNTIRKFWK
CCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHC
GDDGLLPTMAGRFSASSDVFNRRGRRPWASVNFITAHDGFTLADLVSYNGKHNEANGEDN
CCCCCCCHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCC
RDGSDDNNSWNCGAEGPTDDEEINTLRRRQMRNMLATLLLSQGTPMLLAGDEFANSQNGN
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCC
NNAYCQDNALSWLDWDGIDEKARSQIAFVTSLLRLRREHVVFHRTRFFTGSVIPGTEVKD
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHEEEECCCCCCCCCCE
VVWLRPDGEEMTEADWGNSVSQALAIRLSGEAGLTHLDRRGRQQTDDTFLLVLNASHTEV
EEEECCCCCHHHHCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCEEEEEEECCCCEE
VFTLPEGGRWETVIDTMDEDGQSSLPKADGGTIRALPGRSLQVLRCRAAHGRSA
EEECCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
SAPSTPLVWPGQPFPLGATWDGSGTNFALFSAHAEKVELCLFDRAGRKETARIVLPEYT
CCCCCCEECCCCCCCCCEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEECCCHH
DEIWHGYLPDIRPGQMYGYRVYGPYDPNAGHRFNANKLLIDPYAKSLKGEILWHDALFAY
HHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHCCCCCEEEECCEEEE
KLGHPDGDLTFDRRDSARHMPKCEVVDPAFTWGRSMEPHPWHETVIYEMHPRGFTMLHPG
ECCCCCCCEEECCCCCCCCCCCCEEECCHHHCCCCCCCCCCCCEEEEEECCCCEEEECCC
VPEELRGTFDGLAHRRVTDYLTDLGVTAIELLPVHAYVQDRHLIDRGLTNYWGYNSIGFL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCCHHHCCCCCCCCC
APHATYLGHGRTASAFKAFVQKMHDAGLEVILDVVYNHTAEGNHLGPTLSFKGIDNRSYY
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEE
YLMGEDLRHYNNFTGTGNALELRHPRVLGMVMDSLRYWATAMGVDGFRFDLATTLARVEG
EEECCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
PFTEHAGFLDAVAQDPVLSHVKLIAEPWDTGLGGYQVGAFPPGWAEWNDQYRNTIRKFWK
CCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHC
GDDGLLPTMAGRFSASSDVFNRRGRRPWASVNFITAHDGFTLADLVSYNGKHNEANGEDN
CCCCCCCHHHCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCCC
RDGSDDNNSWNCGAEGPTDDEEINTLRRRQMRNMLATLLLSQGTPMLLAGDEFANSQNGN
CCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCC
NNAYCQDNALSWLDWDGIDEKARSQIAFVTSLLRLRREHVVFHRTRFFTGSVIPGTEVKD
CCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHEEEECCCCCCCCCCE
VVWLRPDGEEMTEADWGNSVSQALAIRLSGEAGLTHLDRRGRQQTDDTFLLVLNASHTEV
EEEECCCCCHHHHCCCCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCEEEEEEECCCCEE
VFTLPEGGRWETVIDTMDEDGQSSLPKADGGTIRALPGRSLQVLRCRAAHGRSA
EEECCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]