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The map label for this gene is treZ [H]

Identifier: 83591845

GI number: 83591845

Start: 597429

End: 599249

Strand: Direct

Name: treZ [H]

Synonym: Rru_A0506

Alternate gene names: 83591845

Gene position: 597429-599249 (Clockwise)

Preceding gene: 83591844

Following gene: 83591846

Centisome position: 13.73

GC content: 67.38

Gene sequence:

>1821_bases
ATGACAAGACGGTTTGGCGCCCGGGTCGAGGGCGATGGCGTGCGGTTTTCGCTCTGGGCCCCCGCCGCCGAACGGGTTGA
GGTGGTTATCGAAGGCGCGGCCGAATCGCGCCCCGTCGCCATGGTGCCCGGCGGCGAGGGCTGGTTTGGCGCCATGGTCG
GCGGCATCGGCGCCGGGGCGCTTTATCGCTTCCGCATTGATGGCGCCGATCTGGTGCCCGATCCAGCCTCGCGCTTCCAG
CCCCGGGACGTGGGCGGCCCCAGTCAGGTGGTCGATGCGAGCGGCTATGCCTGGAAGACCGACGGCTGGAAAGGCCGTCC
CTGGGCGGAGACGGTGCTTTACGAACTGCATCTGGGCACCTTCAGCCGTAAGGGAACCTATGAGGGGGCGCGGGCCCGCC
TGGGGGATCTGGTCGATCTGGGGGTTAGCGCCATCGAATTGATGCCGCTGGCCGATTTCGTCGGTCAACGCAATTGGGGG
TATGACGGCGTGTTGCCCTTCGCCCCCGACAGCGTCTATGGCACGCCCGAGCAGTTGAAGGCGTTGATCGACGCCGCCCA
CGGCCTGGGGCTGATGGTTTTTCTTGATGTCGTCTATAATCACTTCGGCCCCGAGGGCAATTGGCTGGGGCGCTATGCGC
CAAGCTTCTTCACCCAGGCCCACCAGACCCCCTGGGGGGCGGCCATCGATTTCGAACGGCCCGAGGTGCGCGATTTCTTC
GTGGCCAATGCTTTGCAATGGCTGGAGGAGTATCGCTTCGACGGCCTGCGCTTCGATGCCGTCCACGCCATCCTCGATGC
CGAGGAACGCCATATCCTGGGCGAGATCGCCGTGGCCGTCCGCGAGGCCTTGCCGGCGGACCGCCACGTCCATCTGGTGC
TGGAAAACGATGCCAATGAAGCCCGCCGGCTGATCGGCGGAACCGACGTGGTGGCGGTGCGCGGGCGTTATGACGCCCAG
TGGAACGATGATATCCACCACGCCTTCCATGTGGTGGCCACCGGCGAGACCGGCGGCTATTACGCCGATTACGCCGCCCA
GCCGCTGGCCCATCTTGGTCGTTGTCTGGCCGAAGGCTTCGCCTATCAAGGCGAGTCCTCGGCCCATCGCGGCGGCATGG
CGCGCGGCGAGCCGACGACGGCGCTGCCGCCGACGGCCTTCGTCGCCTTTCTGCAGAACCACGATCAGATCGGCAATCGG
GCTTTGGGGGAAAGATTAAGCACCCTGGCCGCGCCCGACCGGTTGCGCGCCCTGCGCACGATCTTGCTGCTCGGGCCGCA
GATCCCGATGCTGTTCATGGGCGAGGAATGGTCGAGCTCGCGGCCCTTCCTGTTCTTTTGCGATTTCCACGGCGATCTGG
CCGAGGCGGTGCGCGAGGGGCGTCGGCGGGAATTCGCCGCCTTCCCCCAATTCTCCACCCCGGAAACCCGCGATCGCATT
CCCGATCCCAATGCCCCGGAAACCGCCCGGGCCTGCGTGCTCGACTGGTCCGAGCGCGATCAGCCGACGGGCGCGGCGGT
GCTGGCCCATCACAAACACCTGCTGGAGCTGCGCTTTGCCAAGATTGTGCCCTTGCTGGGCCGGCTGCGGCCGGGGGGCG
GACGCTGCGAGCGCATCGGCGAGGGCGGTCTGGCGGTGCGCTGGGGGATCGACGACGGGCGCGATCTGCTGATGCTGGCC
CAGCTTGCCGAGGCCGGATCATTGCCCGGCGCCCCGCCGCAGGCCGATTTGATCTATCGCAGCGACGGCGGCGAGCGGGC
CGAAGCGCCCCTTGGTCCGTGGAGCGTGCTTTTCGCCCTGGTGCCGGAGACGAGCCGATGA

Upstream 100 bases:

>100_bases
GCCAGTCGAGTCTGCCCAAAGCGGATGGCGGGACCATCCGCGCCCTGCCCGGGCGCAGCCTTCAGGTGCTGCGCTGCCGC
GCCGCCCATGGGAGATCGGC

Downstream 100 bases:

>100_bases
CCGCCGATCTCTATGCGCCGACCGCCACCTATCGGCTGCAGTTCCATGCCGGGTTCACCTTCACCGATGCCGCCGCCCAG
GTTGGCTATCTGCGCGATCT

Product: glycoside hydrolase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MTRRFGARVEGDGVRFSLWAPAAERVEVVIEGAAESRPVAMVPGGEGWFGAMVGGIGAGALYRFRIDGADLVPDPASRFQ
PRDVGGPSQVVDASGYAWKTDGWKGRPWAETVLYELHLGTFSRKGTYEGARARLGDLVDLGVSAIELMPLADFVGQRNWG
YDGVLPFAPDSVYGTPEQLKALIDAAHGLGLMVFLDVVYNHFGPEGNWLGRYAPSFFTQAHQTPWGAAIDFERPEVRDFF
VANALQWLEEYRFDGLRFDAVHAILDAEERHILGEIAVAVREALPADRHVHLVLENDANEARRLIGGTDVVAVRGRYDAQ
WNDDIHHAFHVVATGETGGYYADYAAQPLAHLGRCLAEGFAYQGESSAHRGGMARGEPTTALPPTAFVAFLQNHDQIGNR
ALGERLSTLAAPDRLRALRTILLLGPQIPMLFMGEEWSSSRPFLFFCDFHGDLAEAVREGRRREFAAFPQFSTPETRDRI
PDPNAPETARACVLDWSERDQPTGAAVLAHHKHLLELRFAKIVPLLGRLRPGGGRCERIGEGGLAVRWGIDDGRDLLMLA
QLAEAGSLPGAPPQADLIYRSDGGERAEAPLGPWSVLFALVPETSR

Sequences:

>Translated_606_residues
MTRRFGARVEGDGVRFSLWAPAAERVEVVIEGAAESRPVAMVPGGEGWFGAMVGGIGAGALYRFRIDGADLVPDPASRFQ
PRDVGGPSQVVDASGYAWKTDGWKGRPWAETVLYELHLGTFSRKGTYEGARARLGDLVDLGVSAIELMPLADFVGQRNWG
YDGVLPFAPDSVYGTPEQLKALIDAAHGLGLMVFLDVVYNHFGPEGNWLGRYAPSFFTQAHQTPWGAAIDFERPEVRDFF
VANALQWLEEYRFDGLRFDAVHAILDAEERHILGEIAVAVREALPADRHVHLVLENDANEARRLIGGTDVVAVRGRYDAQ
WNDDIHHAFHVVATGETGGYYADYAAQPLAHLGRCLAEGFAYQGESSAHRGGMARGEPTTALPPTAFVAFLQNHDQIGNR
ALGERLSTLAAPDRLRALRTILLLGPQIPMLFMGEEWSSSRPFLFFCDFHGDLAEAVREGRRREFAAFPQFSTPETRDRI
PDPNAPETARACVLDWSERDQPTGAAVLAHHKHLLELRFAKIVPLLGRLRPGGGRCERIGEGGLAVRWGIDDGRDLLMLA
QLAEAGSLPGAPPQADLIYRSDGGERAEAPLGPWSVLFALVPETSR
>Mature_605_residues
TRRFGARVEGDGVRFSLWAPAAERVEVVIEGAAESRPVAMVPGGEGWFGAMVGGIGAGALYRFRIDGADLVPDPASRFQP
RDVGGPSQVVDASGYAWKTDGWKGRPWAETVLYELHLGTFSRKGTYEGARARLGDLVDLGVSAIELMPLADFVGQRNWGY
DGVLPFAPDSVYGTPEQLKALIDAAHGLGLMVFLDVVYNHFGPEGNWLGRYAPSFFTQAHQTPWGAAIDFERPEVRDFFV
ANALQWLEEYRFDGLRFDAVHAILDAEERHILGEIAVAVREALPADRHVHLVLENDANEARRLIGGTDVVAVRGRYDAQW
NDDIHHAFHVVATGETGGYYADYAAQPLAHLGRCLAEGFAYQGESSAHRGGMARGEPTTALPPTAFVAFLQNHDQIGNRA
LGERLSTLAAPDRLRALRTILLLGPQIPMLFMGEEWSSSRPFLFFCDFHGDLAEAVREGRRREFAAFPQFSTPETRDRIP
DPNAPETARACVLDWSERDQPTGAAVLAHHKHLLELRFAKIVPLLGRLRPGGGRCERIGEGGLAVRWGIDDGRDLLMLAQ
LAEAGSLPGAPPQADLIYRSDGGERAEAPLGPWSVLFALVPETSR

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=165, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI2367229, Length=524, Percent_Identity=25.381679389313, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789839, Length=281, Percent_Identity=30.2491103202847, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554896, Length=172, Percent_Identity=34.3023255813954, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI32564391, Length=172, Percent_Identity=34.3023255813954, Blast_Score=89, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320826, Length=167, Percent_Identity=33.5329341317365, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28573410, Length=211, Percent_Identity=28.9099526066351, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR022567
- InterPro:   IPR012768 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48; PF11941 DUF3459 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 66341; Mature: 66209

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRFGARVEGDGVRFSLWAPAAERVEVVIEGAAESRPVAMVPGGEGWFGAMVGGIGAGA
CCCCCCCEECCCCEEEEEECCCHHEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCE
LYRFRIDGADLVPDPASRFQPRDVGGPSQVVDASGYAWKTDGWKGRPWAETVLYELHLGT
EEEEEECCCCCCCCCHHHCCCCCCCCCHHEEECCCCEEECCCCCCCCHHHHHEEHEECCC
FSRKGTYEGARARLGDLVDLGVSAIELMPLADFVGQRNWGYDGVLPFAPDSVYGTPEQLK
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
ALIDAAHGLGLMVFLDVVYNHFGPEGNWLGRYAPSFFTQAHQTPWGAAIDFERPEVRDFF
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCCHHHHHH
VANALQWLEEYRFDGLRFDAVHAILDAEERHILGEIAVAVREALPADRHVHLVLENDANE
HHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHH
ARRLIGGTDVVAVRGRYDAQWNDDIHHAFHVVATGETGGYYADYAAQPLAHLGRCLAEGF
HHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHH
AYQGESSAHRGGMARGEPTTALPPTAFVAFLQNHDQIGNRALGERLSTLAAPDRLRALRT
CCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ILLLGPQIPMLFMGEEWSSSRPFLFFCDFHGDLAEAVREGRRREFAAFPQFSTPETRDRI
HHHHCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
PDPNAPETARACVLDWSERDQPTGAAVLAHHKHLLELRFAKIVPLLGRLRPGGGRCERIG
CCCCCCHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEC
EGGLAVRWGIDDGRDLLMLAQLAEAGSLPGAPPQADLIYRSDGGERAEAPLGPWSVLFAL
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEE
VPETSR
CCCCCC
>Mature Secondary Structure 
TRRFGARVEGDGVRFSLWAPAAERVEVVIEGAAESRPVAMVPGGEGWFGAMVGGIGAGA
CCCCCCEECCCCEEEEEECCCHHEEEEEEECCCCCCCEEEECCCCCHHHHHHHCCCCCE
LYRFRIDGADLVPDPASRFQPRDVGGPSQVVDASGYAWKTDGWKGRPWAETVLYELHLGT
EEEEEECCCCCCCCCHHHCCCCCCCCCHHEEECCCCEEECCCCCCCCHHHHHEEHEECCC
FSRKGTYEGARARLGDLVDLGVSAIELMPLADFVGQRNWGYDGVLPFAPDSVYGTPEQLK
CCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHH
ALIDAAHGLGLMVFLDVVYNHFGPEGNWLGRYAPSFFTQAHQTPWGAAIDFERPEVRDFF
HHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCCHHHHHH
VANALQWLEEYRFDGLRFDAVHAILDAEERHILGEIAVAVREALPADRHVHLVLENDANE
HHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHH
ARRLIGGTDVVAVRGRYDAQWNDDIHHAFHVVATGETGGYYADYAAQPLAHLGRCLAEGF
HHHHHCCCCEEEECCCCCCCCCCCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHH
AYQGESSAHRGGMARGEPTTALPPTAFVAFLQNHDQIGNRALGERLSTLAAPDRLRALRT
CCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ILLLGPQIPMLFMGEEWSSSRPFLFFCDFHGDLAEAVREGRRREFAAFPQFSTPETRDRI
HHHHCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHCC
PDPNAPETARACVLDWSERDQPTGAAVLAHHKHLLELRFAKIVPLLGRLRPGGGRCERIG
CCCCCCHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEC
EGGLAVRWGIDDGRDLLMLAQLAEAGSLPGAPPQADLIYRSDGGERAEAPLGPWSVLFAL
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEE
VPETSR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]