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The map label for this gene is ilvI [H]

Identifier: 83591807

GI number: 83591807

Start: 547496

End: 549256

Strand: Direct

Name: ilvI [H]

Synonym: Rru_A0467

Alternate gene names: 83591807

Gene position: 547496-549256 (Clockwise)

Preceding gene: 83591806

Following gene: 83591808

Centisome position: 12.58

GC content: 62.63

Gene sequence:

>1761_bases
ATGGCTGACCAGCAGAAGTCCGGTGCGGAGATCGTCCTTAAGGCTCTTGCCGATCAGGGTGTGGAGGTAATCTTCGGCTA
TCCGGGCGGCGCCGTCCTTCCGCTCTATGACGAGATCTTCAAGCAAAATCAGATCCGTCACGTGCTGGTCCGTCACGAAC
AGGCCGCCGTCCACGCCGCCGAAGGCTATGCCCGCTCGACCGGCAAGACCGGCGTGGTCCTGGTGACCAGCGGCCCGGGC
GCCACCAATGCGGTGACCGGCCTGACCGACGCCATGCTCGATTCCATTCCCGTGGTCTGCCTGACCGGTCAGGTGCCGAC
CCATCTGATCGGCAACGATGCCTTTCAGGAGGCCGACACCACCGGCATCACCCGCCAGTGCACCAAGCACAATTATCTGG
TGAAAAAGGGCGAGGATCTGGCCCGGGTGATCCACGAGGCCTTCCAGGTCGCCGGCAGCGGCCGGCCCGGCCCGGTGGTC
ATCGATCTGCCCAAGGATATCCTGATGGGACCGGCGCCCTATGTCGCCCCGTCGCGGGTCAAGCACAAAAGCTACCGTCC
CCAGCTCAAGGGCGATATCACCGCCATTGAAAAGGCGGTCGAGATCATCGCCAAGGCCAAGCGGCCGATTTTCTATTGCG
GCGGCGGCGTCGTCAATTCCGGGCCCCAGGCCAGCCAGTTGCTGACCCAGTTCGTGAAGATGACCGGCTATCCGATTACC
CTGACCCTGATGGGGCTGGGCATCTATCCGGCCAGCGATCCGCAATATCTGGGCATGCTGGGCATGCATGGCACCTTCGA
GGCCAATCACGCCATGCATGACTGCGACGTGATGGTGGCGATCGGGGCGCGGTTCGACGACCGGGTGACCGGCCGGCTTG
ACGCCTTCTCGCCCAATTCGACCAAGATCCAGATCGACATCGATCCCAGTTCGATCAACAAGAACGTGGCGGTCGATCTG
GGGATCGTCGGCGATGTCGGTCATGTCCTTGAAGACATGATCAAGGTGTGGAAGGCCCGGCAGTATCACCCCGATGTCGC
CGCCCTGAAAAACTGGTGGGGGCGGATCGCCGATTGGCAGCGCCGCGACTGCCTGCACTATCAGCCCTCCGACGAGGTTA
CCAAGCCCCAATACGCCATCGAGCGCCTCTACGCCCTGACCCGCGACCGCGAGACCTTCATCACCACCGAGGTCGGCCAG
CATCAGATGTGGGCGGCCCAGTACTTCAAATTCGACAAGCCCAATCAGTGGATGACCTCGGGCGGGCTCGGCACCATGGG
CTATGGTTTCCCGGCGGCGATCGGCGTTCAGATGGCCCATCCCGGCGCCCTGGTCGTCGATATCTCGGGCGAGGCGTCGT
TCATGATGAACATGCAGGAACTGGCCACGGCCGTGCAGTATCGCCTGCCGGTCAAGGTGTTCATCCTCAATAACCGGTAC
ATGGGCATGGTTCGCCAGTGGCAGGAACTGCTCCATGGCGGGCGTTATTCGGAAAGCTATTCCGAATGCCTGCCCGATTT
CGTCAAGCTGGCCGAGGCCTTCGGCGCCGTCGGCTTGCGGTCGGAAAAGCCCGCCGATCTTGATGACGTGATCGCCGAGA
TGATCGCCGTCGATCGGCCGGTGCTGGTCGATGTGGCGGTCGATCCTTCGGAAAACGTCTTTCCGATGATCCCCTCGGGC
GCGCCCCACAACCAGATGCTGCTCGGCCCCGAAGACAGCCTGGGTCAGCCGATTTCCAAGGAGGGGGCGATCCTCGTCTA
G

Upstream 100 bases:

>100_bases
CCGTCCTTGGATGGAAGGCCGTTTCCCGATCCCTGGCCGCTCCCCGGGCAACCGCGGGGGGACGCGGGGGGAAAGAACAT
CGTCTGACACAGGTGTGACC

Downstream 100 bases:

>100_bases
ATCCCGCGCCCCCGCTCCGGGGGTGTCGTCCAGCCTTTTCCCACCCTGTTATCCCAAGGGAGCCCCCCGGCGCCGGTTGA
TCGGCGGCGGGGCGCGGCCT

Product: acetolactate synthase, large subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MADQQKSGAEIVLKALADQGVEVIFGYPGGAVLPLYDEIFKQNQIRHVLVRHEQAAVHAAEGYARSTGKTGVVLVTSGPG
ATNAVTGLTDAMLDSIPVVCLTGQVPTHLIGNDAFQEADTTGITRQCTKHNYLVKKGEDLARVIHEAFQVAGSGRPGPVV
IDLPKDILMGPAPYVAPSRVKHKSYRPQLKGDITAIEKAVEIIAKAKRPIFYCGGGVVNSGPQASQLLTQFVKMTGYPIT
LTLMGLGIYPASDPQYLGMLGMHGTFEANHAMHDCDVMVAIGARFDDRVTGRLDAFSPNSTKIQIDIDPSSINKNVAVDL
GIVGDVGHVLEDMIKVWKARQYHPDVAALKNWWGRIADWQRRDCLHYQPSDEVTKPQYAIERLYALTRDRETFITTEVGQ
HQMWAAQYFKFDKPNQWMTSGGLGTMGYGFPAAIGVQMAHPGALVVDISGEASFMMNMQELATAVQYRLPVKVFILNNRY
MGMVRQWQELLHGGRYSESYSECLPDFVKLAEAFGAVGLRSEKPADLDDVIAEMIAVDRPVLVDVAVDPSENVFPMIPSG
APHNQMLLGPEDSLGQPISKEGAILV

Sequences:

>Translated_586_residues
MADQQKSGAEIVLKALADQGVEVIFGYPGGAVLPLYDEIFKQNQIRHVLVRHEQAAVHAAEGYARSTGKTGVVLVTSGPG
ATNAVTGLTDAMLDSIPVVCLTGQVPTHLIGNDAFQEADTTGITRQCTKHNYLVKKGEDLARVIHEAFQVAGSGRPGPVV
IDLPKDILMGPAPYVAPSRVKHKSYRPQLKGDITAIEKAVEIIAKAKRPIFYCGGGVVNSGPQASQLLTQFVKMTGYPIT
LTLMGLGIYPASDPQYLGMLGMHGTFEANHAMHDCDVMVAIGARFDDRVTGRLDAFSPNSTKIQIDIDPSSINKNVAVDL
GIVGDVGHVLEDMIKVWKARQYHPDVAALKNWWGRIADWQRRDCLHYQPSDEVTKPQYAIERLYALTRDRETFITTEVGQ
HQMWAAQYFKFDKPNQWMTSGGLGTMGYGFPAAIGVQMAHPGALVVDISGEASFMMNMQELATAVQYRLPVKVFILNNRY
MGMVRQWQELLHGGRYSESYSECLPDFVKLAEAFGAVGLRSEKPADLDDVIAEMIAVDRPVLVDVAVDPSENVFPMIPSG
APHNQMLLGPEDSLGQPISKEGAILV
>Mature_585_residues
ADQQKSGAEIVLKALADQGVEVIFGYPGGAVLPLYDEIFKQNQIRHVLVRHEQAAVHAAEGYARSTGKTGVVLVTSGPGA
TNAVTGLTDAMLDSIPVVCLTGQVPTHLIGNDAFQEADTTGITRQCTKHNYLVKKGEDLARVIHEAFQVAGSGRPGPVVI
DLPKDILMGPAPYVAPSRVKHKSYRPQLKGDITAIEKAVEIIAKAKRPIFYCGGGVVNSGPQASQLLTQFVKMTGYPITL
TLMGLGIYPASDPQYLGMLGMHGTFEANHAMHDCDVMVAIGARFDDRVTGRLDAFSPNSTKIQIDIDPSSINKNVAVDLG
IVGDVGHVLEDMIKVWKARQYHPDVAALKNWWGRIADWQRRDCLHYQPSDEVTKPQYAIERLYALTRDRETFITTEVGQH
QMWAAQYFKFDKPNQWMTSGGLGTMGYGFPAAIGVQMAHPGALVVDISGEASFMMNMQELATAVQYRLPVKVFILNNRYM
GMVRQWQELLHGGRYSESYSECLPDFVKLAEAFGAVGLRSEKPADLDDVIAEMIAVDRPVLVDVAVDPSENVFPMIPSGA
PHNQMLLGPEDSLGQPISKEGAILV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=24.7386759581882, Blast_Score=164, Evalue=3e-40,
Organism=Homo sapiens, GI21361361, Length=552, Percent_Identity=26.4492753623188, Blast_Score=152, Evalue=9e-37,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=52.2887323943662, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1790104, Length=563, Percent_Identity=41.2078152753108, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786717, Length=557, Percent_Identity=34.6499102333932, Blast_Score=299, Evalue=4e-82,
Organism=Escherichia coli, GI1787096, Length=554, Percent_Identity=29.7833935018051, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788716, Length=573, Percent_Identity=25.4799301919721, Blast_Score=162, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=534, Percent_Identity=26.9662921348315, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=534, Percent_Identity=26.9662921348315, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=520, Percent_Identity=25.3846153846154, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6323755, Length=579, Percent_Identity=45.4231433506045, Blast_Score=483, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6320816, Length=477, Percent_Identity=24.7379454926625, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6321524, Length=537, Percent_Identity=21.7877094972067, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323163, Length=537, Percent_Identity=21.0428305400372, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323073, Length=538, Percent_Identity=20.817843866171, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19922626, Length=562, Percent_Identity=25.0889679715303, Blast_Score=166, Evalue=6e-41,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63940; Mature: 63809

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQQKSGAEIVLKALADQGVEVIFGYPGGAVLPLYDEIFKQNQIRHVLVRHEQAAVHAA
CCCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHH
EGYARSTGKTGVVLVTSGPGATNAVTGLTDAMLDSIPVVCLTGQVPTHLIGNDAFQEADT
CCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHCCC
TGITRQCTKHNYLVKKGEDLARVIHEAFQVAGSGRPGPVVIDLPKDILMGPAPYVAPSRV
CCCHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCCCHH
KHKSYRPQLKGDITAIEKAVEIIAKAKRPIFYCGGGVVNSGPQASQLLTQFVKMTGYPIT
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEE
LTLMGLGIYPASDPQYLGMLGMHGTFEANHAMHDCDVMVAIGARFDDRVTGRLDAFSPNS
EEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCC
TKIQIDIDPSSINKNVAVDLGIVGDVGHVLEDMIKVWKARQYHPDVAALKNWWGRIADWQ
CEEEEEECHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHH
RRDCLHYQPSDEVTKPQYAIERLYALTRDRETFITTEVGQHQMWAAQYFKFDKPNQWMTS
HCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCEECC
GGLGTMGYGFPAAIGVQMAHPGALVVDISGEASFMMNMQELATAVQYRLPVKVFILNNRY
CCCCCCCCCCHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCH
MGMVRQWQELLHGGRYSESYSECLPDFVKLAEAFGAVGLRSEKPADLDDVIAEMIAVDRP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
VLVDVAVDPSENVFPMIPSGAPHNQMLLGPEDSLGQPISKEGAILV
EEEEEEECCCCCEEEECCCCCCCCCEEECCCHHCCCCCCCCCCEEC
>Mature Secondary Structure 
ADQQKSGAEIVLKALADQGVEVIFGYPGGAVLPLYDEIFKQNQIRHVLVRHEQAAVHAA
CCCCCCCHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHH
EGYARSTGKTGVVLVTSGPGATNAVTGLTDAMLDSIPVVCLTGQVPTHLIGNDAFQEADT
CCHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHCCCHHHHHCCC
TGITRQCTKHNYLVKKGEDLARVIHEAFQVAGSGRPGPVVIDLPKDILMGPAPYVAPSRV
CCCHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHCCCCCCCCCCHH
KHKSYRPQLKGDITAIEKAVEIIAKAKRPIFYCGGGVVNSGPQASQLLTQFVKMTGYPIT
HHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCEE
LTLMGLGIYPASDPQYLGMLGMHGTFEANHAMHDCDVMVAIGARFDDRVTGRLDAFSPNS
EEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCCCC
TKIQIDIDPSSINKNVAVDLGIVGDVGHVLEDMIKVWKARQYHPDVAALKNWWGRIADWQ
CEEEEEECHHHCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHH
RRDCLHYQPSDEVTKPQYAIERLYALTRDRETFITTEVGQHQMWAAQYFKFDKPNQWMTS
HCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCEECC
GGLGTMGYGFPAAIGVQMAHPGALVVDISGEASFMMNMQELATAVQYRLPVKVFILNNRY
CCCCCCCCCCHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEEECCCH
MGMVRQWQELLHGGRYSESYSECLPDFVKLAEAFGAVGLRSEKPADLDDVIAEMIAVDRP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
VLVDVAVDPSENVFPMIPSGAPHNQMLLGPEDSLGQPISKEGAILV
EEEEEEECCCCCEEEECCCCCCCCCEEECCCHHCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]