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The map label for this gene is miaA

Identifier: 83591806

GI number: 83591806

Start: 546394

End: 547374

Strand: Direct

Name: miaA

Synonym: Rru_A0466

Alternate gene names: 83591806

Gene position: 546394-547374 (Clockwise)

Preceding gene: 83591804

Following gene: 83591807

Centisome position: 12.55

GC content: 70.74

Gene sequence:

>981_bases
ATGATCGACCCCCTCGCTTCCCCGGGCTCCGCCCCCTTGATCGTCATCGCTGGACCGACCGCCTCGGGCAAGTCGGCTTT
GGCCCTGCGGGTGGCGCGGCGCTTCAACGGCGTTGTCATCAATGCCGACAGCATGCAGGTCTACCGCGACCTGCGGATCC
TCAGCGCCCGTCCCAGTGTCGAAGACGAGGCGGCCGCTCCCCATGCCCTTTATGGCGTGCTTGATGGGGCGGAGGTTTGT
TCGGTCGGCCGCTGGCTGGCCCTGGCCGACGGCGCCGTGGCGGCGGCGCGGGCCGCCGGGCGTCTGCCGATCCTCTGCGG
GGGAACCGGGCTTTATCTGAAGGCGGCGCTTGAGGGGCTGGCCAGCGTGCCCGAGGTGGCGCCCGAGGTTCGCGCCGAGG
CCCGCGAGCTGCTGGCGGCCCTGGGGCCGGCGGCCTTGCATGCCAAACTGGCCGAGCTGGATCGGGCGATGGCCGAGCGC
CTGCGGCCCAGCGACGCCCAGCGCATCGCCCGGGCCTATGAGGTGGTGCGCGGCACCGGGCGTTCGCTGATGGACTGGTG
GAGCGATCCGGTCGCCACCCCGGCCCTGCCCGGACGGCCGTTGCTGCTGGTGATCGATCCGCCGCGCGCCGCGCAGCGGG
CGGCCTGTGACGCGCGGCTTTTGGCGATGGTCGCCGCCGGGGCGCTGGACGAACTGGCGCTTCTGCTGGCGCGCCGGCTT
GATCCGGCCCTGCCGGTGATGCGGGCGGTCGGGGTTCCCGAACTGGCCCGGACCCTGCGCGGCGAGGTCGGTCTGGCCGA
GGCGGTGGAGCGGGCGCAGGGCGCGACCCGGCGCTATGCCAAGCGTCAGGGCACCTGGATCCGTACCCAGCTCAGGCCGG
ACCGGGTTCTTTCGGAATCCCTGGTCACGCAATTATCGGAAAGCCAGCTAACGGACATTGATAATTTTCTGTATGCCTTT
TTATTGACCGGCGCATCTTGA

Upstream 100 bases:

>100_bases
GGCGTTGCTCCCCCAGGATGATGCCGCGCCTTCCTAGCCGGTTCGGGGGGGCAGGGACAAGGGGCTTTGGCTTGGCGAGG
CGAACCGTTAGCTTAAGGCC

Downstream 100 bases:

>100_bases
CGGACTAGGCTGCCCTCCCTTCCGTCCTTGGATGGAAGGCCGTTTCCCGATCCCTGGCCGCTCCCCGGGCAACCGCGGGG
GGACGCGGGGGGAAAGAACA

Product: tRNA isopentenyltransferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MIDPLASPGSAPLIVIAGPTASGKSALALRVARRFNGVVINADSMQVYRDLRILSARPSVEDEAAAPHALYGVLDGAEVC
SVGRWLALADGAVAAARAAGRLPILCGGTGLYLKAALEGLASVPEVAPEVRAEARELLAALGPAALHAKLAELDRAMAER
LRPSDAQRIARAYEVVRGTGRSLMDWWSDPVATPALPGRPLLLVIDPPRAAQRAACDARLLAMVAAGALDELALLLARRL
DPALPVMRAVGVPELARTLRGEVGLAEAVERAQGATRRYAKRQGTWIRTQLRPDRVLSESLVTQLSESQLTDIDNFLYAF
LLTGAS

Sequences:

>Translated_326_residues
MIDPLASPGSAPLIVIAGPTASGKSALALRVARRFNGVVINADSMQVYRDLRILSARPSVEDEAAAPHALYGVLDGAEVC
SVGRWLALADGAVAAARAAGRLPILCGGTGLYLKAALEGLASVPEVAPEVRAEARELLAALGPAALHAKLAELDRAMAER
LRPSDAQRIARAYEVVRGTGRSLMDWWSDPVATPALPGRPLLLVIDPPRAAQRAACDARLLAMVAAGALDELALLLARRL
DPALPVMRAVGVPELARTLRGEVGLAEAVERAQGATRRYAKRQGTWIRTQLRPDRVLSESLVTQLSESQLTDIDNFLYAF
LLTGAS
>Mature_326_residues
MIDPLASPGSAPLIVIAGPTASGKSALALRVARRFNGVVINADSMQVYRDLRILSARPSVEDEAAAPHALYGVLDGAEVC
SVGRWLALADGAVAAARAAGRLPILCGGTGLYLKAALEGLASVPEVAPEVRAEARELLAALGPAALHAKLAELDRAMAER
LRPSDAQRIARAYEVVRGTGRSLMDWWSDPVATPALPGRPLLLVIDPPRAAQRAACDARLLAMVAAGALDELALLLARRL
DPALPVMRAVGVPELARTLRGEVGLAEAVERAQGATRRYAKRQGTWIRTQLRPDRVLSESLVTQLSESQLTDIDNFLYAF
LLTGAS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=25.8064516129032, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1790613, Length=277, Percent_Identity=38.2671480144404, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI25144712, Length=303, Percent_Identity=23.4323432343234, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=29.2929292929293, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24649830, Length=236, Percent_Identity=33.8983050847458, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_RHORT (Q2RX74)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_425558.1
- ProteinModelPortal:   Q2RX74
- SMR:   Q2RX74
- STRING:   Q2RX74
- GeneID:   3834069
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0466
- NMPDR:   fig|1085.1.peg.1869
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK745172
- BioCyc:   RRUB269796:RRU_A0466-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34475; Mature: 34475

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDPLASPGSAPLIVIAGPTASGKSALALRVARRFNGVVINADSMQVYRDLRILSARPSV
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCC
EDEAAAPHALYGVLDGAEVCSVGRWLALADGAVAAARAAGRLPILCGGTGLYLKAALEGL
CCHHCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCHHHHHHHHHHH
ASVPEVAPEVRAEARELLAALGPAALHAKLAELDRAMAERLRPSDAQRIARAYEVVRGTG
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
RSLMDWWSDPVATPALPGRPLLLVIDPPRAAQRAACDARLLAMVAAGALDELALLLARRL
HHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPALPVMRAVGVPELARTLRGEVGLAEAVERAQGATRRYAKRQGTWIRTQLRPDRVLSES
CHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
LVTQLSESQLTDIDNFLYAFLLTGAS
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDPLASPGSAPLIVIAGPTASGKSALALRVARRFNGVVINADSMQVYRDLRILSARPSV
CCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCC
EDEAAAPHALYGVLDGAEVCSVGRWLALADGAVAAARAAGRLPILCGGTGLYLKAALEGL
CCHHCCCHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCHHHHHHHHHHH
ASVPEVAPEVRAEARELLAALGPAALHAKLAELDRAMAERLRPSDAQRIARAYEVVRGTG
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
RSLMDWWSDPVATPALPGRPLLLVIDPPRAAQRAACDARLLAMVAAGALDELALLLARRL
HHHHHHHCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPALPVMRAVGVPELARTLRGEVGLAEAVERAQGATRRYAKRQGTWIRTQLRPDRVLSES
CHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHH
LVTQLSESQLTDIDNFLYAFLLTGAS
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA