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The map label for this gene is yfcG [H]

Identifier: 83591672

GI number: 83591672

Start: 404156

End: 404845

Strand: Direct

Name: yfcG [H]

Synonym: Rru_A0332

Alternate gene names: 83591672

Gene position: 404156-404845 (Clockwise)

Preceding gene: 83591671

Following gene: 83591674

Centisome position: 9.28

GC content: 63.77

Gene sequence:

>690_bases
ATGTATGATCTGTATTACTGGCCGACACCCAATGGCTTCAAGATTTCCATCCTGCTTGAAGAACTGGGGCAGCCCTATAC
CCTGAAGCCGGTCAATATCGCCAAGGGCGAGCAGTTCGCCCCCGAGTTCCTGGCGATCAGCCCGAACAATCGCATGCCGG
CCCTGGTCGACCATGCGCCAAGCGACGGGGGGGCGGCGATCGCGCTGTTCGAATCCGGGGCGATCTTGGAGTATCTGGCG
ACCAAGCATGGCGCCTTCCTGCCCAGCGATATCCGCGGCCGCACCGAAACCATGGTCTGGTTGTTCTGGCAGGTCGGCGG
CCTGGGGCCGATGGCCGGTCAGGCCCACCATTTCCGCCAATACGCTCCCGAACCGGTCCCTTACGCCATCGAGCGCTATA
CCAACGAGGTCAACCGTCTTTATGGGGTGATGAACAAGCGCTTGGCCGATCGCGACTATCTGGCCGGGGCCTATTCGATC
GCCGATATGGCGTCCTGGCCCTGGGTGGTGTCCCATGCCAATCAGGGGCAGTCGCTCGACGACTTCCCCAACCTCAAGCG
CTGGTTCGACGCCATCGGCGCCCGGCCGGCGGTGCAACGCGGCATGGCGCTGGGGGCGGACCTGCGCGAAGCCAATGCCA
ATACCACGCCCGAGGCGCGGGCCGTGCTGTTTGGCCAGAAGGCCCGTTGA

Upstream 100 bases:

>100_bases
GCTTAGGCGCGGCCGGCGGGCCGAGGCTTGATTGGCGGCGCAGGCCACCAAATATCGGGCGCGTTCCCTCTTGTCCGTTT
CCGCTCCAGGAGCCGCCCCG

Downstream 100 bases:

>100_bases
TCAAGGCCCGTTGATCAAGGCCCGTTGATCAAGGTCCGTTGATCAAGGCCCGCTGACCGGGGCCGACGCCCGCCCTTTTG
GCGGAAACGACCCCGGGCCG

Product: glutathione S-transferase-like protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MYDLYYWPTPNGFKISILLEELGQPYTLKPVNIAKGEQFAPEFLAISPNNRMPALVDHAPSDGGAAIALFESGAILEYLA
TKHGAFLPSDIRGRTETMVWLFWQVGGLGPMAGQAHHFRQYAPEPVPYAIERYTNEVNRLYGVMNKRLADRDYLAGAYSI
ADMASWPWVVSHANQGQSLDDFPNLKRWFDAIGARPAVQRGMALGADLREANANTTPEARAVLFGQKAR

Sequences:

>Translated_229_residues
MYDLYYWPTPNGFKISILLEELGQPYTLKPVNIAKGEQFAPEFLAISPNNRMPALVDHAPSDGGAAIALFESGAILEYLA
TKHGAFLPSDIRGRTETMVWLFWQVGGLGPMAGQAHHFRQYAPEPVPYAIERYTNEVNRLYGVMNKRLADRDYLAGAYSI
ADMASWPWVVSHANQGQSLDDFPNLKRWFDAIGARPAVQRGMALGADLREANANTTPEARAVLFGQKAR
>Mature_229_residues
MYDLYYWPTPNGFKISILLEELGQPYTLKPVNIAKGEQFAPEFLAISPNNRMPALVDHAPSDGGAAIALFESGAILEYLA
TKHGAFLPSDIRGRTETMVWLFWQVGGLGPMAGQAHHFRQYAPEPVPYAIERYTNEVNRLYGVMNKRLADRDYLAGAYSI
ADMASWPWVVSHANQGQSLDDFPNLKRWFDAIGARPAVQRGMALGADLREANANTTPEARAVLFGQKAR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=216, Percent_Identity=53.7037037037037, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI87082195, Length=212, Percent_Identity=43.3962264150943, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI87081789, Length=169, Percent_Identity=28.9940828402367, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324100, Length=233, Percent_Identity=33.4763948497854, Blast_Score=125, Evalue=7e-30,
Organism=Drosophila melanogaster, GI28571670, Length=175, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17864598, Length=194, Percent_Identity=29.3814432989691, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17864592, Length=195, Percent_Identity=26.6666666666667, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24646249, Length=195, Percent_Identity=28.7179487179487, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 25443; Mature: 25443

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDLYYWPTPNGFKISILLEELGQPYTLKPVNIAKGEQFAPEFLAISPNNRMPALVDHAP
CCCEEEECCCCCEEEEEEHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCHHEECCC
SDGGAAIALFESGAILEYLATKHGAFLPSDIRGRTETMVWLFWQVGGLGPMAGQAHHFRQ
CCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCCCCCCCHHHHHH
YAPEPVPYAIERYTNEVNRLYGVMNKRLADRDYLAGAYSIADMASWPWVVSHANQGQSLD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
DFPNLKRWFDAIGARPAVQRGMALGADLREANANTTPEARAVLFGQKAR
CCHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCCHHEEEECCCCC
>Mature Secondary Structure
MYDLYYWPTPNGFKISILLEELGQPYTLKPVNIAKGEQFAPEFLAISPNNRMPALVDHAP
CCCEEEECCCCCEEEEEEHHHHCCCEEECCCCCCCCCCCCCCEEEECCCCCCCHHEECCC
SDGGAAIALFESGAILEYLATKHGAFLPSDIRGRTETMVWLFWQVGGLGPMAGQAHHFRQ
CCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCHHEEEEEEEECCCCCCCCCCHHHHHH
YAPEPVPYAIERYTNEVNRLYGVMNKRLADRDYLAGAYSIADMASWPWVVSHANQGQSLD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
DFPNLKRWFDAIGARPAVQRGMALGADLREANANTTPEARAVLFGQKAR
CCHHHHHHHHHHCCCHHHHHHHHHCCCHHHCCCCCCCCHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]