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The map label for this gene is hydN [H]

Identifier: 83591663

GI number: 83591663

Start: 392138

End: 392668

Strand: Direct

Name: hydN [H]

Synonym: Rru_A0323

Alternate gene names: 83591663

Gene position: 392138-392668 (Clockwise)

Preceding gene: 83591662

Following gene: 83591664

Centisome position: 9.01

GC content: 64.6

Gene sequence:

>531_bases
GTGAACAAGTTCGTCATCGCCGACCCCAAGAAATGCATTGGGTGTCGCACCTGCGAAGTCGCCTGCGTCGTCGCCCATTC
CAACGGCAAGGGTGGCGCCAATTCGATGTCGGAGGAGAGCTTTTCTCCCCGGCTGCGGGTGATCAAGACCGCCACCGTGA
CCACGCCGATCCAGTGCCACCACTGCGACGACGCTCCCTGCCTCAACGCCTGCCCGAACGGTGCGATCGTTTATTCGCAT
GACTCCGTGCAGGTCAATCAGGCCCGCTGCATGGGCTGCAAGAACTGCGTCATGGCCTGCCCCTTCGGCGCCATGCAGGT
GGTGAAGGCGTCGGCCCATGGCAACGACGGCGATAGCCGCCGGGTCGAGGCCCATAAGTGCGACCTGTGCATCGAGCGCG
CCGCCGGTCCGGCCTGTATCGACGCCTGTCCGACCAAATCCCTTCATCTTGTCGATCGCGAGCGGGTTCAGGAGGTGCTG
CGCAAGCGCCAGCTCCAGGCCGCCCTCGACGCCTCGGGCATGTCCCTCTGA

Upstream 100 bases:

>100_bases
AGCGCGGCCGTGAGCCGGACGCTGGCCTCCCTGTCTGACCTACTCAAGGAGCTTTTGAAATGACGAGCGCAAACCAAAGG
GCGCTAACTGGAGGGCAACC

Downstream 100 bases:

>100_bases
GCGCGACAAGCACATCCATCGCCTAAAGGAGTAGAGAAGATGGAAAAGGTTTTGACCGTCTGCCCGTATTGCGGCTCGGG
GTGCAAGATCAACCTGCTCG

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MNKFVIADPKKCIGCRTCEVACVVAHSNGKGGANSMSEESFSPRLRVIKTATVTTPIQCHHCDDAPCLNACPNGAIVYSH
DSVQVNQARCMGCKNCVMACPFGAMQVVKASAHGNDGDSRRVEAHKCDLCIERAAGPACIDACPTKSLHLVDRERVQEVL
RKRQLQAALDASGMSL

Sequences:

>Translated_176_residues
MNKFVIADPKKCIGCRTCEVACVVAHSNGKGGANSMSEESFSPRLRVIKTATVTTPIQCHHCDDAPCLNACPNGAIVYSH
DSVQVNQARCMGCKNCVMACPFGAMQVVKASAHGNDGDSRRVEAHKCDLCIERAAGPACIDACPTKSLHLVDRERVQEVL
RKRQLQAALDASGMSL
>Mature_176_residues
MNKFVIADPKKCIGCRTCEVACVVAHSNGKGGANSMSEESFSPRLRVIKTATVTTPIQCHHCDDAPCLNACPNGAIVYSH
DSVQVNQARCMGCKNCVMACPFGAMQVVKASAHGNDGDSRRVEAHKCDLCIERAAGPACIDACPTKSLHLVDRERVQEVL
RKRQLQAALDASGMSL

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=174, Percent_Identity=49.4252873563218, Blast_Score=168, Evalue=2e-43,
Organism=Escherichia coli, GI2367245, Length=164, Percent_Identity=50, Blast_Score=163, Evalue=7e-42,
Organism=Escherichia coli, GI1788811, Length=168, Percent_Identity=44.6428571428571, Blast_Score=160, Evalue=4e-41,
Organism=Escherichia coli, GI87082179, Length=163, Percent_Identity=42.9447852760736, Blast_Score=150, Evalue=6e-38,
Organism=Escherichia coli, GI87082180, Length=169, Percent_Identity=40.8284023668639, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI87082114, Length=201, Percent_Identity=35.8208955223881, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789079, Length=201, Percent_Identity=39.8009950248756, Blast_Score=125, Evalue=1e-30,
Organism=Escherichia coli, GI1787749, Length=189, Percent_Identity=31.2169312169312, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI1790326, Length=177, Percent_Identity=31.0734463276836, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787122, Length=185, Percent_Identity=30.2702702702703, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787872, Length=167, Percent_Identity=29.940119760479, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI2367345, Length=169, Percent_Identity=28.4023668639053, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI226510944, Length=178, Percent_Identity=27.5280898876405, Blast_Score=63, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 18830; Mature: 18830

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
12.5 %Cys+Met (Translated Protein)
9.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
12.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFVIADPKKCIGCRTCEVACVVAHSNGKGGANSMSEESFSPRLRVIKTATVTTPIQCH
CCCEEECCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECCEEEE
HCDDAPCLNACPNGAIVYSHDSVQVNQARCMGCKNCVMACPFGAMQVVKASAHGNDGDSR
CCCCCCCCCCCCCCCEEEECCCEEECHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCCCCC
RVEAHKCDLCIERAAGPACIDACPTKSLHLVDRERVQEVLRKRQLQAALDASGMSL
EEHHHHHHHHHHHCCCCCEECCCCCCCEEHHCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKFVIADPKKCIGCRTCEVACVVAHSNGKGGANSMSEESFSPRLRVIKTATVTTPIQCH
CCCEEECCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEEECCEEEE
HCDDAPCLNACPNGAIVYSHDSVQVNQARCMGCKNCVMACPFGAMQVVKASAHGNDGDSR
CCCCCCCCCCCCCCCEEEECCCEEECHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCCCCC
RVEAHKCDLCIERAAGPACIDACPTKSLHLVDRERVQEVLRKRQLQAALDASGMSL
EEHHHHHHHHHHHCCCCCEECCCCCCCEEHHCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]