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The map label for this gene is hyfE [H]

Identifier: 83591659

GI number: 83591659

Start: 388811

End: 389476

Strand: Direct

Name: hyfE [H]

Synonym: Rru_A0319

Alternate gene names: 83591659

Gene position: 388811-389476 (Clockwise)

Preceding gene: 83591658

Following gene: 83591660

Centisome position: 8.93

GC content: 60.96

Gene sequence:

>666_bases
ATGGCTAGCGGCGCGACGATCTATATAAGTCTTCTTCTTATCGTAACCTCGTTTGTCGTTGTCGAATGGCGTTATCTGAA
AGGGGCGATCTTCACCTATCAGTGCCAGTCGGTGCTGATGGCCCTGATGTTCGTTCTCTACGGCACGGCGCTTGATAACC
ACGCCCTTTATTATTGGGCCGGCACCGCGCTGGTCAGCAAGGGGGTGATCATTCCCTGGCTGCTGCGCCAATACGTGAAC
AAGGTCGGCGCCGAGGAGACCCGGCCGATGATGGGGGCGGCGCTGTCGTTCATCCTCGCCGTGGTCGTCGCCGCCCTGGC
CTTCTGGTGGACCTTCAACCACCACGACGATCTGGTCATCACCGCCGAGCTGATCGGCGAGCCCTACCGGCTCAATCTGG
CGGTGGCGGCGGTGGTTCTGGTGATCGGCTTCTACGCCCTGCTCACCCGCCGCGACGCCTTCAAGATCGTCATCGGTCTG
TGCCTTCTGGAAAACGCCGTCCACCTGAGCCTCGCCAGCCTGGCGCCGACCATCGCCGAGACGGCGATGATCGGGGTGGT
CACCGATGTCGTGATCTCGGTGTGGATGATGCTTTACGTCGTCCGCTCGATCTACGGCGCCGTCGGCTCCACCGACACCA
CCAATATTTCGCAGCTGAATGGCTGA

Upstream 100 bases:

>100_bases
CCGTGGATGAACAAGAAGATCGATGTTCCCAAGTGGCTTTGGCAGTGCCCGCAATGCCGGCTTGACAAGACCGGCTCCGT
GATCGAAGGGAGCATCAAAA

Downstream 100 bases:

>100_bases
GGGAGCCGGGACAATGGCGAATTCATCCTTACTACTTCTGTTATTCCTGATTCCCGCCCTGGGGGCGGTCGTGGAATATC
CCTTGGGCCGCGCCCGCTCG

Product: NADH-ubiquinone oxidoreductase, chain 4L

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MASGATIYISLLLIVTSFVVVEWRYLKGAIFTYQCQSVLMALMFVLYGTALDNHALYYWAGTALVSKGVIIPWLLRQYVN
KVGAEETRPMMGAALSFILAVVVAALAFWWTFNHHDDLVITAELIGEPYRLNLAVAAVVLVIGFYALLTRRDAFKIVIGL
CLLENAVHLSLASLAPTIAETAMIGVVTDVVISVWMMLYVVRSIYGAVGSTDTTNISQLNG

Sequences:

>Translated_221_residues
MASGATIYISLLLIVTSFVVVEWRYLKGAIFTYQCQSVLMALMFVLYGTALDNHALYYWAGTALVSKGVIIPWLLRQYVN
KVGAEETRPMMGAALSFILAVVVAALAFWWTFNHHDDLVITAELIGEPYRLNLAVAAVVLVIGFYALLTRRDAFKIVIGL
CLLENAVHLSLASLAPTIAETAMIGVVTDVVISVWMMLYVVRSIYGAVGSTDTTNISQLNG
>Mature_220_residues
ASGATIYISLLLIVTSFVVVEWRYLKGAIFTYQCQSVLMALMFVLYGTALDNHALYYWAGTALVSKGVIIPWLLRQYVNK
VGAEETRPMMGAALSFILAVVVAALAFWWTFNHHDDLVITAELIGEPYRLNLAVAAVVLVIGFYALLTRRDAFKIVIGLC
LLENAVHLSLASLAPTIAETAMIGVVTDVVISVWMMLYVVRSIYGAVGSTDTTNISQLNG

Specific function: Unknown

COG id: COG4237

COG function: function code C; Hydrogenase 4 membrane component (E)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788830, Length=219, Percent_Identity=23.7442922374429, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001133 [H]

Pfam domain/function: PF00420 Oxidored_q2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24186; Mature: 24055

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGATIYISLLLIVTSFVVVEWRYLKGAIFTYQCQSVLMALMFVLYGTALDNHALYYWA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
GTALVSKGVIIPWLLRQYVNKVGAEETRPMMGAALSFILAVVVAALAFWWTFNHHDDLVI
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TAELIGEPYRLNLAVAAVVLVIGFYALLTRRDAFKIVIGLCLLENAVHLSLASLAPTIAE
EHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAMIGVVTDVVISVWMMLYVVRSIYGAVGSTDTTNISQLNG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASGATIYISLLLIVTSFVVVEWRYLKGAIFTYQCQSVLMALMFVLYGTALDNHALYYWA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
GTALVSKGVIIPWLLRQYVNKVGAEETRPMMGAALSFILAVVVAALAFWWTFNHHDDLVI
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TAELIGEPYRLNLAVAAVVLVIGFYALLTRRDAFKIVIGLCLLENAVHLSLASLAPTIAE
EHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAMIGVVTDVVISVWMMLYVVRSIYGAVGSTDTTNISQLNG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]