Click here to switch to the map view.

The map label for this gene is hycF [H]

Identifier: 83591658

GI number: 83591658

Start: 388291

End: 388818

Strand: Direct

Name: hycF [H]

Synonym: Rru_A0318

Alternate gene names: 83591658

Gene position: 388291-388818 (Clockwise)

Preceding gene: 83591657

Following gene: 83591659

Centisome position: 8.92

GC content: 63.07

Gene sequence:

>528_bases
ATGCTGAGCAAAATCAAAGAAGCCCTGATCTGCCTGCGGGCCGGGCGCGTCACCCTTCCTTATCCCTTCGTGCCGCTGAA
GGCCCCGCCGCGCTTTCGCGGCCGGCCGACCATCGATGGCGCCAAATGCATCGGCTGCGGCGCCTGCGCCGAGGTCTGTC
CGCCCCGGCTGATCGAGGTCAACGACGCGGCGAGCACCCGCACGGTCGAGTTGAACTATTCGCGCTGCACCTATTGCGCG
CGGTGCCAGGAAATCTGCCCGACCGGGGCGATGACCTGCACCGAGGACTTCGAGATGGCGACGGCCGACCGCAAGAACCT
GACGGTCTCGGTCCAGCTCGACATGGTCCACTGCGAGACCTGCGGCAAGGCCTTCATGACCAAGCGCATGCTCGACAAGA
TGCTGACCGAGTTCTGTCCGCCGTGGATGAACAAGAAGATCGATGTTCCCAAGTGGCTTTGGCAGTGCCCGCAATGCCGG
CTTGACAAGACCGGCTCCGTGATCGAAGGGAGCATCAAAAATGGCTAG

Upstream 100 bases:

>100_bases
CCCGTTTCAAAATCCATCAAAGCATCCGCTATTTCTACGCGGTGGTCGCTTTCTCGGTCGGGGGTCTGGTCTTGGCGGTC
ATGGGTCTGTAGGGGGCATC

Downstream 100 bases:

>100_bases
CGGCGCGACGATCTATATAAGTCTTCTTCTTATCGTAACCTCGTTTGTCGTTGTCGAATGGCGTTATCTGAAAGGGGCGA
TCTTCACCTATCAGTGCCAG

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 6; Hydrogenase-3 component F [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MLSKIKEALICLRAGRVTLPYPFVPLKAPPRFRGRPTIDGAKCIGCGACAEVCPPRLIEVNDAASTRTVELNYSRCTYCA
RCQEICPTGAMTCTEDFEMATADRKNLTVSVQLDMVHCETCGKAFMTKRMLDKMLTEFCPPWMNKKIDVPKWLWQCPQCR
LDKTGSVIEGSIKNG

Sequences:

>Translated_175_residues
MLSKIKEALICLRAGRVTLPYPFVPLKAPPRFRGRPTIDGAKCIGCGACAEVCPPRLIEVNDAASTRTVELNYSRCTYCA
RCQEICPTGAMTCTEDFEMATADRKNLTVSVQLDMVHCETCGKAFMTKRMLDKMLTEFCPPWMNKKIDVPKWLWQCPQCR
LDKTGSVIEGSIKNG
>Mature_175_residues
MLSKIKEALICLRAGRVTLPYPFVPLKAPPRFRGRPTIDGAKCIGCGACAEVCPPRLIEVNDAASTRTVELNYSRCTYCA
RCQEICPTGAMTCTEDFEMATADRKNLTVSVQLDMVHCETCGKAFMTKRMLDKMLTEFCPPWMNKKIDVPKWLWQCPQCR
LDKTGSVIEGSIKNG

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789075, Length=165, Percent_Identity=26.0606060606061, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788833, Length=122, Percent_Identity=37.7049180327869, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788617, Length=108, Percent_Identity=37.037037037037, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19547; Mature: 19547

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
13.1 %Cys+Met (Translated Protein)
8.6 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
13.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKIKEALICLRAGRVTLPYPFVPLKAPPRFRGRPTIDGAKCIGCGACAEVCPPRLIEV
CHHHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCCCCCCCEECCCCHHHHCCCCEEEE
NDAASTRTVELNYSRCTYCARCQEICPTGAMTCTEDFEMATADRKNLTVSVQLDMVHCET
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEEEEHHH
CGKAFMTKRMLDKMLTEFCPPWMNKKIDVPKWLWQCPQCRLDKTGSVIEGSIKNG
HHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MLSKIKEALICLRAGRVTLPYPFVPLKAPPRFRGRPTIDGAKCIGCGACAEVCPPRLIEV
CHHHHHHHHHHHHCCCEECCCCCEECCCCCCCCCCCCCCCCCEECCCCHHHHCCCCEEEE
NDAASTRTVELNYSRCTYCARCQEICPTGAMTCTEDFEMATADRKNLTVSVQLDMVHCET
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEEEEHHH
CGKAFMTKRMLDKMLTEFCPPWMNKKIDVPKWLWQCPQCRLDKTGSVIEGSIKNG
HHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]