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The map label for this gene is hyfB [H]

Identifier: 83591655

GI number: 83591655

Start: 383654

End: 385678

Strand: Direct

Name: hyfB [H]

Synonym: Rru_A0315

Alternate gene names: 83591655

Gene position: 383654-385678 (Clockwise)

Preceding gene: 83591654

Following gene: 83591656

Centisome position: 8.81

GC content: 64.99

Gene sequence:

>2025_bases
ATGAGCACAACGCAAGTTTTCCTCGGCGCCTTCGCCGTCCTCGCCGCCCTTCCGGTCCTCACCCTGCTGGCCGGAAACCG
CAAGTCCCTGGCGGGGTCGATCACGCTGGTCGGCGTCGGTGTCGCCTGCCTCGCCATGCTCGGGCTGGCCGTCACCGCTC
TGACCGTAGGGCCGATCGCCTTCGATCTGGGAACCGTGCCGCTGTTTGGCCTGACCTCGAGCCTCGCCTTCACCATCGAC
GGCCTGAGCGCGATCTTCGTCATCTTGATTTCCGTGCTCGGCACCGCCTCGGCCCTCTATTCGGTGGGCTATATCGCCCA
TTACCCCGATGAGGACGCCCGGCGCTATTACGTGCCTTTCCCGCTGTTCATCGCCGGCATGCTGATGGTGGCGACGACCT
CGGACTGGTTTTGTTTCTTCTTCGGCTGGGAGTTCATGACCCTGGTTTCCTATTTCCTGGTGACGTTCGAGAACCGGGAT
AAGGAGAACCTGTCGGCGGGCTTCATCTATTTCTTCATGACCCAGCTCACCTCGATGGGGCTGATGTTGGCTATCATCGT
GCTGGGCACCTGGGGCGGGTCGACCTCCTTCGCCGCCGTCGCCAAGACCCTGGGCGCCCTTCAGGCCGAGAATCCGGTCG
CCCTTTACGGGCTGCTTAGCCTGTTCTTCATCGGCTTCGCCACCAAGGCCGGCATGTTCCCGCTGGGCATCTGGCTGCCC
AAGGCCCATCCGGCCGCCCCGGCCAGCGTGTCGGCGCTGCTGTCGGGCGTGATGATCAAGCTTGGCATCTACGGCATGCT
GCGCATCTTCCTGTGGGCCCTGCCGCTGGGCGAGGCCACCCATGTCTGGGGCATGGTGATCACCCTGTTTGGCGTGCTGT
CGATGCTGGTCGGCACCCTGCGCGCCCTGGGCGAACACGATTGCAAGCGCCTGCTGGCCCAGCATTCCATCGGCCAGATG
GGCTATGTGCTGCTTGGCCTCGGCCTGGGTCTGACCTTCCTTGGCAGCAATCCGCTGTTCGCCGCGCTTGGCTTTCTTGG
CTGCCTCTATCATCTGATCAACCACGCCTGCTTCAAGTCGCTGCTGTTTTTCAACACCGGCTCGATCCTCTACCGCGCCG
GCACCCGCGACCTTGATTCGCTGGGTGGCCTGTCGGCGGTGATGCCGCTGACCGCCGGCTGCGCCCTGGTCGGCGCCCTG
TCGATCGCCGGCACCCCGCCGTTCAACGGCTTCGTCAGCAAGTGGCTGCTTTATCAAAGCGCTATCTTCGGCGACGTCAG
CACCCCCGTTTACATCCTGGCCGCCGTCGTCGCCATTTTCATCGGCACCGTCACCCTGGCCTCCTTCGTCAAATACATGG
GCACCGCCTATCTTGGCACCTTGCCCAAGCGCTTCGTCGGCAGCGGCTTTGGCTGCCCGCGGTCGATGGAGGCGGTCGAG
GTGTTCCTGGCCGTGGTCTGTCTGGTGATGGGCGTTTTCCCCGGCCCGGTGGTCGCCACCTTGCTTGGCGTGCTGGATCC
GGCCTTCGCCGGCGCCGGCGTCGATCTTGGCCCCAGCGTGCTGAGCGCCCTGCCGTGGTCGGGGCTGTCGGTCACCTCGG
CCTCGGGCACGACCCTCACCGTCTTCGCGCCGCTGGTGGTGATCGGTGGACTGGCCGTCAGCTTCGCCCTGTCGGCGCTG
ATCCACGGCGCGGTCAAGGTGCCCTCGCGCCCGGCGCAGATCTGGAACTGCGGCGAGGAGATCGACGACGAACTGGTGCG
CTACCGCGCTTCAAGCTTCTACAGCCCGTTTAAGAAGCTGATCGCCCCGGTCTATCGCCAGCACCCGTTCCCCACCCTGT
CGCTGCCGGCGCTGCTGCCCCGGCTCCTCGATCTCGACAAGTGGCTCTATTTCCCGATCGGCGCGCTTTTTCGCAAATCC
AGCAAGGCGATCAGCCGGGCCCATACGGGTGTTCCCCAGCTCTACCTGTCCTGGCAGGTGGCGGGTGGCGCCCTGGCCAT
CGTCTTCGCCCTCTGGCTGATGTGA

Upstream 100 bases:

>100_bases
GCCATGCTGATCCCCCTCATCGTCCTGATGGCCTTCAGCGTGCTCTCCACCGTTTTCGCGCTGCCCATGATCCAACAGTC
GTTCGGGGGGTGAGATCATC

Downstream 100 bases:

>100_bases
GGTCTTGAAAACCATGACGACGCCACAAACCATTCTCGACGACATCAACGCCCGCTTTCCCGGATGCGTGACCCGCGGCC
TCGCCCCCCAGCCCGACCGG

Product: NADH dehydrogenase (quinone)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 674; Mature: 673

Protein sequence:

>674_residues
MSTTQVFLGAFAVLAALPVLTLLAGNRKSLAGSITLVGVGVACLAMLGLAVTALTVGPIAFDLGTVPLFGLTSSLAFTID
GLSAIFVILISVLGTASALYSVGYIAHYPDEDARRYYVPFPLFIAGMLMVATTSDWFCFFFGWEFMTLVSYFLVTFENRD
KENLSAGFIYFFMTQLTSMGLMLAIIVLGTWGGSTSFAAVAKTLGALQAENPVALYGLLSLFFIGFATKAGMFPLGIWLP
KAHPAAPASVSALLSGVMIKLGIYGMLRIFLWALPLGEATHVWGMVITLFGVLSMLVGTLRALGEHDCKRLLAQHSIGQM
GYVLLGLGLGLTFLGSNPLFAALGFLGCLYHLINHACFKSLLFFNTGSILYRAGTRDLDSLGGLSAVMPLTAGCALVGAL
SIAGTPPFNGFVSKWLLYQSAIFGDVSTPVYILAAVVAIFIGTVTLASFVKYMGTAYLGTLPKRFVGSGFGCPRSMEAVE
VFLAVVCLVMGVFPGPVVATLLGVLDPAFAGAGVDLGPSVLSALPWSGLSVTSASGTTLTVFAPLVVIGGLAVSFALSAL
IHGAVKVPSRPAQIWNCGEEIDDELVRYRASSFYSPFKKLIAPVYRQHPFPTLSLPALLPRLLDLDKWLYFPIGALFRKS
SKAISRAHTGVPQLYLSWQVAGGALAIVFALWLM

Sequences:

>Translated_674_residues
MSTTQVFLGAFAVLAALPVLTLLAGNRKSLAGSITLVGVGVACLAMLGLAVTALTVGPIAFDLGTVPLFGLTSSLAFTID
GLSAIFVILISVLGTASALYSVGYIAHYPDEDARRYYVPFPLFIAGMLMVATTSDWFCFFFGWEFMTLVSYFLVTFENRD
KENLSAGFIYFFMTQLTSMGLMLAIIVLGTWGGSTSFAAVAKTLGALQAENPVALYGLLSLFFIGFATKAGMFPLGIWLP
KAHPAAPASVSALLSGVMIKLGIYGMLRIFLWALPLGEATHVWGMVITLFGVLSMLVGTLRALGEHDCKRLLAQHSIGQM
GYVLLGLGLGLTFLGSNPLFAALGFLGCLYHLINHACFKSLLFFNTGSILYRAGTRDLDSLGGLSAVMPLTAGCALVGAL
SIAGTPPFNGFVSKWLLYQSAIFGDVSTPVYILAAVVAIFIGTVTLASFVKYMGTAYLGTLPKRFVGSGFGCPRSMEAVE
VFLAVVCLVMGVFPGPVVATLLGVLDPAFAGAGVDLGPSVLSALPWSGLSVTSASGTTLTVFAPLVVIGGLAVSFALSAL
IHGAVKVPSRPAQIWNCGEEIDDELVRYRASSFYSPFKKLIAPVYRQHPFPTLSLPALLPRLLDLDKWLYFPIGALFRKS
SKAISRAHTGVPQLYLSWQVAGGALAIVFALWLM
>Mature_673_residues
STTQVFLGAFAVLAALPVLTLLAGNRKSLAGSITLVGVGVACLAMLGLAVTALTVGPIAFDLGTVPLFGLTSSLAFTIDG
LSAIFVILISVLGTASALYSVGYIAHYPDEDARRYYVPFPLFIAGMLMVATTSDWFCFFFGWEFMTLVSYFLVTFENRDK
ENLSAGFIYFFMTQLTSMGLMLAIIVLGTWGGSTSFAAVAKTLGALQAENPVALYGLLSLFFIGFATKAGMFPLGIWLPK
AHPAAPASVSALLSGVMIKLGIYGMLRIFLWALPLGEATHVWGMVITLFGVLSMLVGTLRALGEHDCKRLLAQHSIGQMG
YVLLGLGLGLTFLGSNPLFAALGFLGCLYHLINHACFKSLLFFNTGSILYRAGTRDLDSLGGLSAVMPLTAGCALVGALS
IAGTPPFNGFVSKWLLYQSAIFGDVSTPVYILAAVVAIFIGTVTLASFVKYMGTAYLGTLPKRFVGSGFGCPRSMEAVEV
FLAVVCLVMGVFPGPVVATLLGVLDPAFAGAGVDLGPSVLSALPWSGLSVTSASGTTLTVFAPLVVIGGLAVSFALSALI
HGAVKVPSRPAQIWNCGEEIDDELVRYRASSFYSPFKKLIAPVYRQHPFPTLSLPALLPRLLDLDKWLYFPIGALFRKSS
KAISRAHTGVPQLYLSWQVAGGALAIVFALWLM

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=350, Percent_Identity=26.2857142857143, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI251831116, Length=289, Percent_Identity=28.0276816608997, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1788827, Length=428, Percent_Identity=36.9158878504673, Blast_Score=243, Evalue=4e-65,
Organism=Escherichia coli, GI2367154, Length=616, Percent_Identity=29.0584415584416, Blast_Score=171, Evalue=2e-43,
Organism=Escherichia coli, GI1788831, Length=379, Percent_Identity=31.9261213720317, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788829, Length=439, Percent_Identity=30.5239179954442, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1788613, Length=370, Percent_Identity=29.7297297297297, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788614, Length=403, Percent_Identity=28.7841191066998, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI145693160, Length=432, Percent_Identity=26.3888888888889, Blast_Score=99, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 71386; Mature: 71254

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTQVFLGAFAVLAALPVLTLLAGNRKSLAGSITLVGVGVACLAMLGLAVTALTVGPIA
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLGTVPLFGLTSSLAFTIDGLSAIFVILISVLGTASALYSVGYIAHYPDEDARRYYVPF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEECCH
PLFIAGMLMVATTSDWFCFFFGWEFMTLVSYFLVTFENRDKENLSAGFIYFFMTQLTSMG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LMLAIIVLGTWGGSTSFAAVAKTLGALQAENPVALYGLLSLFFIGFATKAGMFPLGIWLP
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECC
KAHPAAPASVSALLSGVMIKLGIYGMLRIFLWALPLGEATHVWGMVITLFGVLSMLVGTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
RALGEHDCKRLLAQHSIGQMGYVLLGLGLGLTFLGSNPLFAALGFLGCLYHLINHACFKS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLFFNTGSILYRAGTRDLDSLGGLSAVMPLTAGCALVGALSIAGTPPFNGFVSKWLLYQS
HHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AIFGDVSTPVYILAAVVAIFIGTVTLASFVKYMGTAYLGTLPKRFVGSGFGCPRSMEAVE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHH
VFLAVVCLVMGVFPGPVVATLLGVLDPAFAGAGVDLGPSVLSALPWSGLSVTSASGTTLT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEECCCCCCHH
VFAPLVVIGGLAVSFALSALIHGAVKVPSRPAQIWNCGEEIDDELVRYRASSFYSPFKKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IAPVYRQHPFPTLSLPALLPRLLDLDKWLYFPIGALFRKSSKAISRAHTGVPQLYLSWQV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
AGGALAIVFALWLM
CCHHHHHHHHHHHC
>Mature Secondary Structure 
STTQVFLGAFAVLAALPVLTLLAGNRKSLAGSITLVGVGVACLAMLGLAVTALTVGPIA
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDLGTVPLFGLTSSLAFTIDGLSAIFVILISVLGTASALYSVGYIAHYPDEDARRYYVPF
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEECCH
PLFIAGMLMVATTSDWFCFFFGWEFMTLVSYFLVTFENRDKENLSAGFIYFFMTQLTSMG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LMLAIIVLGTWGGSTSFAAVAKTLGALQAENPVALYGLLSLFFIGFATKAGMFPLGIWLP
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECC
KAHPAAPASVSALLSGVMIKLGIYGMLRIFLWALPLGEATHVWGMVITLFGVLSMLVGTL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
RALGEHDCKRLLAQHSIGQMGYVLLGLGLGLTFLGSNPLFAALGFLGCLYHLINHACFKS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLFFNTGSILYRAGTRDLDSLGGLSAVMPLTAGCALVGALSIAGTPPFNGFVSKWLLYQS
HHHHCCCCEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AIFGDVSTPVYILAAVVAIFIGTVTLASFVKYMGTAYLGTLPKRFVGSGFGCPRSMEAVE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHH
VFLAVVCLVMGVFPGPVVATLLGVLDPAFAGAGVDLGPSVLSALPWSGLSVTSASGTTLT
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCEEECCCCCCHH
VFAPLVVIGGLAVSFALSALIHGAVKVPSRPAQIWNCGEEIDDELVRYRASSFYSPFKKL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IAPVYRQHPFPTLSLPALLPRLLDLDKWLYFPIGALFRKSSKAISRAHTGVPQLYLSWQV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEE
AGGALAIVFALWLM
CCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]