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The map label for this gene is hycE [H]

Identifier: 83591656

GI number: 83591656

Start: 385692

End: 387377

Strand: Direct

Name: hycE [H]

Synonym: Rru_A0316

Alternate gene names: 83591656

Gene position: 385692-387377 (Clockwise)

Preceding gene: 83591655

Following gene: 83591657

Centisome position: 8.86

GC content: 64.12

Gene sequence:

>1686_bases
ATGACGACGCCACAAACCATTCTCGACGACATCAACGCCCGCTTTCCCGGATGCGTGACCCGCGGCCTCGCCCCCCAGCC
CGACCGGCTTTATCTGCGCACGACCACCGAGCACATCCGGACGCTCGCCGCCTATGTGTTCAACGACCTCAAAGGCCGGC
TGGTCACCGGGGTTTGCACCGACATGGGGCCGGTGACCGGCGAGTACGACATCATCTATGCCTTCTCGTTGGACGCCGAG
GGCATCCTTCTCACCCTCACCGAGCCGACCGACCCCAAGGATCCCAAGGTCCCGACCCTGACCGATCTGATCCCGGGCGC
CGATTGGCACGAGCGCGAGAACTACGACATGCTGGGCGTGGTCGCCGAAGGCCATCCCAATCCGCGCCGCCTGCTGCTGT
CGGAAGACTGGCCCGAAGACCTGTTTCCGATGCGCAAGGACTTCCCCCACGACTTCAAGCCGCCCAAGGCCGAGAAGATC
ACCACGCCTTTGCGCGACCCGTTGGGCAACGAAAGCAAGGCGACGATCATCAGCGTCGGCCCGTTCTTTCCCACCCTGGA
TGAGCCGGCCTATTTCCGCCTGTTCTGCGAGGGCGAGGAGATCATCGGCTCGGATTACCGGGGGTTCTTCTCGCACCGCG
GCATCGAAAAGCTCAGCGATACGGTCCTTGATTATAATCAGGTGCCGTTCATGGCCGAGCGGGTCTGCGGCATCTGCGGC
TTCGTTCACAGCGCCTGCTATTGCATGGCGGTGGAAGACGCCGCCGGCATCGAGATCCCGCCGCGCGCCAAATACATCCG
CTCGATCATGATGGAACTGGAGCGCCTGCACAGCCATCTGCTGTGGCTGGGGCTTGCCGGCCATTACCTGGGCTTCGACA
CGGTGCTCATGCAGTCGTGGCGCATCCGCGAGCCGATCATGTGGCTGGTCGAGGAGATCACCGGCAACCGCAAGACCTAT
GGCATGAATCTGGTGGGCGGCGTGCGCCGCGATCTTGATAGCGCCATCTGCGACAAGATCATGGCCGCCGTCACCAAGAT
CGGCATCGAATGCGAAGAGCTGATCTCGGCGGTGGCCGGCGATGAGTCGCTGAAGATGCGCATGGTCAAGGTCGGCGTGC
TCAGCCACGAGGACGCGCGCGGCATCTGCGTGGTCGGCCCGACCGCCCGCGCCTCGGGCGTCCATATCGACGCCCGCGCC
GATTACCCCTATGCCGCCTTCCCCGATCTCGATTTCAAGCCGTCGTTCCACGAGGGCGGCGACATCTGGGCCAGAACCCT
GGTGCGGGTCGACGAGGTGCGCTCCTCGGTCGATCTGATCAAGCAGATGATCACCAAGCTGCCCGAGGGCGAGATCATGG
CCGGCTTCGGCCCGATCCCGGCCTGGCGCGAGGGCTATGGCATCGCCGAGGCGCCGCGCGGCGAATGCGTCCATTACGTC
CAGACCGGCAAGGACAACCGTCCCTACCGCTGGCGGGTGCGCGCGGCGACCTATCCGCAGCTTCAGGCGGTGCCCTTGAT
GCTCAAGGGCATGAGCATCGGTGATTTCCCGATCATCATCGGCAGCATCGATCCCTGCTTCTCGTGCACCGAACGCGTGC
TCACGGTCGATACGAAAAGCAAAGCCATCCGCACCTACACCGAAAAAGACCTGCTGGCGCTGACCCGTCGGGCGGGGGGC
AGGTAA

Upstream 100 bases:

>100_bases
GCCCATACGGGTGTTCCCCAGCTCTACCTGTCCTGGCAGGTGGCGGGTGGCGCCCTGGCCATCGTCTTCGCCCTCTGGCT
GATGTGAGGTCTTGAAAACC

Downstream 100 bases:

>100_bases
TCGTGGAAAACGTCGGACTCGCGATCTTCAACGTTCTTTTGGTGGTCTGCGCCGCGCCGCTGCTTGATGGCGTTCTGCGT
CAGGTCAAGGCCCGCATCCA

Product: NADH-ubiquinone oxidoreductase

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MTTPQTILDDINARFPGCVTRGLAPQPDRLYLRTTTEHIRTLAAYVFNDLKGRLVTGVCTDMGPVTGEYDIIYAFSLDAE
GILLTLTEPTDPKDPKVPTLTDLIPGADWHERENYDMLGVVAEGHPNPRRLLLSEDWPEDLFPMRKDFPHDFKPPKAEKI
TTPLRDPLGNESKATIISVGPFFPTLDEPAYFRLFCEGEEIIGSDYRGFFSHRGIEKLSDTVLDYNQVPFMAERVCGICG
FVHSACYCMAVEDAAGIEIPPRAKYIRSIMMELERLHSHLLWLGLAGHYLGFDTVLMQSWRIREPIMWLVEEITGNRKTY
GMNLVGGVRRDLDSAICDKIMAAVTKIGIECEELISAVAGDESLKMRMVKVGVLSHEDARGICVVGPTARASGVHIDARA
DYPYAAFPDLDFKPSFHEGGDIWARTLVRVDEVRSSVDLIKQMITKLPEGEIMAGFGPIPAWREGYGIAEAPRGECVHYV
QTGKDNRPYRWRVRAATYPQLQAVPLMLKGMSIGDFPIIIGSIDPCFSCTERVLTVDTKSKAIRTYTEKDLLALTRRAGG
R

Sequences:

>Translated_561_residues
MTTPQTILDDINARFPGCVTRGLAPQPDRLYLRTTTEHIRTLAAYVFNDLKGRLVTGVCTDMGPVTGEYDIIYAFSLDAE
GILLTLTEPTDPKDPKVPTLTDLIPGADWHERENYDMLGVVAEGHPNPRRLLLSEDWPEDLFPMRKDFPHDFKPPKAEKI
TTPLRDPLGNESKATIISVGPFFPTLDEPAYFRLFCEGEEIIGSDYRGFFSHRGIEKLSDTVLDYNQVPFMAERVCGICG
FVHSACYCMAVEDAAGIEIPPRAKYIRSIMMELERLHSHLLWLGLAGHYLGFDTVLMQSWRIREPIMWLVEEITGNRKTY
GMNLVGGVRRDLDSAICDKIMAAVTKIGIECEELISAVAGDESLKMRMVKVGVLSHEDARGICVVGPTARASGVHIDARA
DYPYAAFPDLDFKPSFHEGGDIWARTLVRVDEVRSSVDLIKQMITKLPEGEIMAGFGPIPAWREGYGIAEAPRGECVHYV
QTGKDNRPYRWRVRAATYPQLQAVPLMLKGMSIGDFPIIIGSIDPCFSCTERVLTVDTKSKAIRTYTEKDLLALTRRAGG
R
>Mature_560_residues
TTPQTILDDINARFPGCVTRGLAPQPDRLYLRTTTEHIRTLAAYVFNDLKGRLVTGVCTDMGPVTGEYDIIYAFSLDAEG
ILLTLTEPTDPKDPKVPTLTDLIPGADWHERENYDMLGVVAEGHPNPRRLLLSEDWPEDLFPMRKDFPHDFKPPKAEKIT
TPLRDPLGNESKATIISVGPFFPTLDEPAYFRLFCEGEEIIGSDYRGFFSHRGIEKLSDTVLDYNQVPFMAERVCGICGF
VHSACYCMAVEDAAGIEIPPRAKYIRSIMMELERLHSHLLWLGLAGHYLGFDTVLMQSWRIREPIMWLVEEITGNRKTYG
MNLVGGVRRDLDSAICDKIMAAVTKIGIECEELISAVAGDESLKMRMVKVGVLSHEDARGICVVGPTARASGVHIDARAD
YPYAAFPDLDFKPSFHEGGDIWARTLVRVDEVRSSVDLIKQMITKLPEGEIMAGFGPIPAWREGYGIAEAPRGECVHYVQ
TGKDNRPYRWRVRAATYPQLQAVPLMLKGMSIGDFPIIIGSIDPCFSCTERVLTVDTKSKAIRTYTEKDLLALTRRAGGR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=408, Percent_Identity=26.7156862745098, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI260898743, Length=398, Percent_Identity=26.6331658291457, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1789076, Length=554, Percent_Identity=40.2527075812274, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1788832, Length=551, Percent_Identity=38.2940108892922, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI145693162, Length=484, Percent_Identity=27.6859504132231, Blast_Score=189, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17555284, Length=386, Percent_Identity=28.4974093264249, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17568379, Length=386, Percent_Identity=28.4974093264249, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI221459469, Length=395, Percent_Identity=28.1012658227848, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24638644, Length=406, Percent_Identity=26.3546798029557, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 62707; Mature: 62576

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00542 COMPLEX1_30K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPQTILDDINARFPGCVTRGLAPQPDRLYLRTTTEHIRTLAAYVFNDLKGRLVTGVCT
CCCHHHHHHHHCCCCCCHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEHH
DMGPVTGEYDIIYAFSLDAEGILLTLTEPTDPKDPKVPTLTDLIPGADWHERENYDMLGV
CCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEE
VAEGHPNPRRLLLSEDWPEDLFPMRKDFPHDFKPPKAEKITTPLRDPLGNESKATIISVG
EECCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCHHCCCCHHCCCCCCCCEEEEEEC
PFFPTLDEPAYFRLFCEGEEIIGSDYRGFFSHRGIEKLSDTVLDYNQVPFMAERVCGICG
CCCCCCCCCCEEEEEECCCHHHCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
FVHSACYCMAVEDAAGIEIPPRAKYIRSIMMELERLHSHLLWLGLAGHYLGFDTVLMQSW
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RIREPIMWLVEEITGNRKTYGMNLVGGVRRDLDSAICDKIMAAVTKIGIECEELISAVAG
CCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
DESLKMRMVKVGVLSHEDARGICVVGPTARASGVHIDARADYPYAAFPDLDFKPSFHEGG
CHHHEEEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCH
DIWARTLVRVDEVRSSVDLIKQMITKLPEGEIMAGFGPIPAWREGYGIAEAPRGECVHYV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHCCCCCCCCCCCCEEEHH
QTGKDNRPYRWRVRAATYPQLQAVPLMLKGMSIGDFPIIIGSIDPCFSCTERVLTVDTKS
HCCCCCCCEEEEEEECCCCCHHHCHHHEECCCCCCCEEEEECCCHHHHHHHHEEEECCCH
KAIRTYTEKDLLALTRRAGGR
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTPQTILDDINARFPGCVTRGLAPQPDRLYLRTTTEHIRTLAAYVFNDLKGRLVTGVCT
CCHHHHHHHHCCCCCCHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEHH
DMGPVTGEYDIIYAFSLDAEGILLTLTEPTDPKDPKVPTLTDLIPGADWHERENYDMLGV
CCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEEE
VAEGHPNPRRLLLSEDWPEDLFPMRKDFPHDFKPPKAEKITTPLRDPLGNESKATIISVG
EECCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCHHCCCCHHCCCCCCCCEEEEEEC
PFFPTLDEPAYFRLFCEGEEIIGSDYRGFFSHRGIEKLSDTVLDYNQVPFMAERVCGICG
CCCCCCCCCCEEEEEECCCHHHCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
FVHSACYCMAVEDAAGIEIPPRAKYIRSIMMELERLHSHLLWLGLAGHYLGFDTVLMQSW
HHHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RIREPIMWLVEEITGNRKTYGMNLVGGVRRDLDSAICDKIMAAVTKIGIECEELISAVAG
CCCHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
DESLKMRMVKVGVLSHEDARGICVVGPTARASGVHIDARADYPYAAFPDLDFKPSFHEGG
CHHHEEEEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCH
DIWARTLVRVDEVRSSVDLIKQMITKLPEGEIMAGFGPIPAWREGYGIAEAPRGECVHYV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHCCCCCCCCCCCCEEEHH
QTGKDNRPYRWRVRAATYPQLQAVPLMLKGMSIGDFPIIIGSIDPCFSCTERVLTVDTKS
HCCCCCCCEEEEEEECCCCCHHHCHHHEECCCCCCCEEEEECCCHHHHHHHHEEEECCCH
KAIRTYTEKDLLALTRRAGGR
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]