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The map label for this gene is nodE [H]

Identifier: 83591385

GI number: 83591385

Start: 46946

End: 48160

Strand: Direct

Name: nodE [H]

Synonym: Rru_A0045

Alternate gene names: 83591385

Gene position: 46946-48160 (Clockwise)

Preceding gene: 83591384

Following gene: 83591386

Centisome position: 1.08

GC content: 64.77

Gene sequence:

>1215_bases
ATGCGGCGCGTCGTGGTTACGGGCATGGGGGCGGTTTCGGCGATCGGAGCCAATGTTGGGGAATTCGCCGAGGCCCTGCG
CCAGGGGCGGAGTGGGATCGGTCCGATCGAAGCCATCTCCACCGACCGGCTGACCGTCAAGATCGCCGCCGAAGTGAAGG
GCTTCGATCCCAAGGCCCATTTCACCTCTGACAAGCTGGCGATGCTCGATCGCTTCGCCCAGCTCGCCCTGGTCGCCGCC
CGCGAGGCGATGGCCGATAGCGCGTTGACCATCGACGAGGATCTGGCGCCGCGCTGCGCCTGCATCATTGGCAGCGGTGG
CAGCGGTCTGATCACCCTCGAAGAGGGCTACCGCCGCCTCTATGAAGAGGGCAAGCACCGGGTGCCGCCCTTTACCGTGC
CCAAATACATGAACAATTCGGCGACCAGTCAGGTGACTATGGAATTCGGGCTGAAGGGCCCGGCTTTCTCGGTATCGAGC
GCCTGCGCCACCGCCACCCATTCCATCGGCCTCGGCTTTCACATGGTGCGGTCGGGCATGGCCGATGTCGCCGTGGTCGG
CGGCGCCGACGCCCTGATCACCATGGGCCATATCAAGGCCTGGGAAGCCCTGCGCGTCGTCGCCAAGGATACCTGCCGGC
CCTTCTGCAAGGATCGCAGCGGCATGGTCCTGGCCGAAGGCGCGGCGGTTTTCGTGCTGGAAACCCTGGAAAGCGCCCAG
AAGCGCGGGGCGACCATCCATGCCGAAATGGCTGGATTTGGCATGACCGCCGATGCCGGCGATCTGCTCAGCCCCTCGGT
CGACGGGGCGAGCCGCGCCGTTCGCATGGCTTTGGCCGATGGCGGCTTGGCCCCGGAAGACATCGCCTATGTGAACGCCC
ATGGAACCGGAACGGTGGCCAATGACGCGACCGAAACCAAATGCCTGCACGAGGTTTTCGGCGACCATGCCCACAAGCTG
ATGATTTCGGCGACGAAATCGTTCCACGGTCATTCGCTGGGGGCGTCTGGCGCGGTGGAACTGGTGGCGACGATTCTGGC
GTTGCGCGATGGCCTGATCGCTCCGACGCTGAATTTCACCGAGCCCGATCCCGAATGCGATCTGGACTACGTGCCCAATG
TGGCTCGTCAGGTCACGGTTGACGCGGGGATTTCCAACAGCTTCGCCTTCGGTGGCCTGAACGCGGTGCTGGCCCTGCGG
CGGTTCGTCGGCTGA

Upstream 100 bases:

>100_bases
GCCGGATCGGGCCCGCTTGAGTTTCGACCCGGCGACGGGTTCGGGCTTGGGGTGTCGGGGAAGGCTTTTACTTGGAAACA
TGGGCAAGGCGGGTATGAGC

Downstream 100 bases:

>100_bases
CGGAGATCGGTTCTTGGCTCCGCTACCGGTAACCCCCCGTATCCTCGTCAGCGGTTGTCTGCTGGGCGAGCCCATCCGTT
ATAACGCGACCGCCAAGCCG

Product: 3-oxoacyl-[acyl-carrier-protein] synthase II

Products: [acyl carrier protein]; acetoacetyl-ACP; CO2; [acyl carrier protein]; beta-ketoacyl-ACP [C]

Alternate protein names: Host-specificity of nodulation protein B [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MRRVVVTGMGAVSAIGANVGEFAEALRQGRSGIGPIEAISTDRLTVKIAAEVKGFDPKAHFTSDKLAMLDRFAQLALVAA
REAMADSALTIDEDLAPRCACIIGSGGSGLITLEEGYRRLYEEGKHRVPPFTVPKYMNNSATSQVTMEFGLKGPAFSVSS
ACATATHSIGLGFHMVRSGMADVAVVGGADALITMGHIKAWEALRVVAKDTCRPFCKDRSGMVLAEGAAVFVLETLESAQ
KRGATIHAEMAGFGMTADAGDLLSPSVDGASRAVRMALADGGLAPEDIAYVNAHGTGTVANDATETKCLHEVFGDHAHKL
MISATKSFHGHSLGASGAVELVATILALRDGLIAPTLNFTEPDPECDLDYVPNVARQVTVDAGISNSFAFGGLNAVLALR
RFVG

Sequences:

>Translated_404_residues
MRRVVVTGMGAVSAIGANVGEFAEALRQGRSGIGPIEAISTDRLTVKIAAEVKGFDPKAHFTSDKLAMLDRFAQLALVAA
REAMADSALTIDEDLAPRCACIIGSGGSGLITLEEGYRRLYEEGKHRVPPFTVPKYMNNSATSQVTMEFGLKGPAFSVSS
ACATATHSIGLGFHMVRSGMADVAVVGGADALITMGHIKAWEALRVVAKDTCRPFCKDRSGMVLAEGAAVFVLETLESAQ
KRGATIHAEMAGFGMTADAGDLLSPSVDGASRAVRMALADGGLAPEDIAYVNAHGTGTVANDATETKCLHEVFGDHAHKL
MISATKSFHGHSLGASGAVELVATILALRDGLIAPTLNFTEPDPECDLDYVPNVARQVTVDAGISNSFAFGGLNAVLALR
RFVG
>Mature_404_residues
MRRVVVTGMGAVSAIGANVGEFAEALRQGRSGIGPIEAISTDRLTVKIAAEVKGFDPKAHFTSDKLAMLDRFAQLALVAA
REAMADSALTIDEDLAPRCACIIGSGGSGLITLEEGYRRLYEEGKHRVPPFTVPKYMNNSATSQVTMEFGLKGPAFSVSS
ACATATHSIGLGFHMVRSGMADVAVVGGADALITMGHIKAWEALRVVAKDTCRPFCKDRSGMVLAEGAAVFVLETLESAQ
KRGATIHAEMAGFGMTADAGDLLSPSVDGASRAVRMALADGGLAPEDIAYVNAHGTGTVANDATETKCLHEVFGDHAHKL
MISATKSFHGHSLGASGAVELVATILALRDGLIAPTLNFTEPDPECDLDYVPNVARQVTVDAGISNSFAFGGLNAVLALR
RFVG

Specific function: Proposed to synthesize NOD factor fatty acyl chain. Involved in the synthesis of a highly unsaturated fatty acid moiety, which forms part of a lipo-oligosaccharide that is responsible for host specificity [H]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

Organism=Homo sapiens, GI8923559, Length=420, Percent_Identity=37.1428571428571, Blast_Score=245, Evalue=6e-65,
Organism=Homo sapiens, GI223972627, Length=147, Percent_Identity=44.8979591836735, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI41872631, Length=433, Percent_Identity=26.5588914549654, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1787337, Length=410, Percent_Identity=40.9756097560976, Blast_Score=274, Evalue=6e-75,
Organism=Escherichia coli, GI1788663, Length=419, Percent_Identity=34.8448687350835, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI71983541, Length=414, Percent_Identity=31.8840579710145, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI71983535, Length=263, Percent_Identity=34.6007604562738, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI212642053, Length=357, Percent_Identity=27.1708683473389, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17550940, Length=402, Percent_Identity=26.6169154228856, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6320904, Length=445, Percent_Identity=33.7078651685393, Blast_Score=209, Evalue=4e-55,
Organism=Drosophila melanogaster, GI24644341, Length=421, Percent_Identity=35.6294536817102, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19920632, Length=373, Percent_Identity=26.2734584450402, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI221330659, Length=271, Percent_Identity=29.520295202952, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24581345, Length=438, Percent_Identity=25.1141552511416, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 42164; Mature: 42164

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00606 B_KETOACYL_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRVVVTGMGAVSAIGANVGEFAEALRQGRSGIGPIEAISTDRLTVKIAAEVKGFDPKAH
CCEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCHHEECCCCEEEEEEEEECCCCCCCC
FTSDKLAMLDRFAQLALVAAREAMADSALTIDEDLAPRCACIIGSGGSGLITLEEGYRRL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCEEEEHHHHHHHH
YEEGKHRVPPFTVPKYMNNSATSQVTMEFGLKGPAFSVSSACATATHSIGLGFHMVRSGM
HHCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHHCCC
ADVAVVGGADALITMGHIKAWEALRVVAKDTCRPFCKDRSGMVLAEGAAVFVLETLESAQ
CCEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCEEHHHHHHHHH
KRGATIHAEMAGFGMTADAGDLLSPSVDGASRAVRMALADGGLAPEDIAYVNAHGTGTVA
HCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHEEEHHHCCCCCCCCEEEEECCCCCCCC
NDATETKCLHEVFGDHAHKLMISATKSFHGHSLGASGAVELVATILALRDGLIAPTLNFT
CCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
EPDPECDLDYVPNVARQVTVDAGISNSFAFGGLNAVLALRRFVG
CCCCCCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRRVVVTGMGAVSAIGANVGEFAEALRQGRSGIGPIEAISTDRLTVKIAAEVKGFDPKAH
CCEEEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCHHEECCCCEEEEEEEEECCCCCCCC
FTSDKLAMLDRFAQLALVAAREAMADSALTIDEDLAPRCACIIGSGGSGLITLEEGYRRL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCEEEEEECCCCEEEEHHHHHHHH
YEEGKHRVPPFTVPKYMNNSATSQVTMEFGLKGPAFSVSSACATATHSIGLGFHMVRSGM
HHCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCHHHHHHHCCC
ADVAVVGGADALITMGHIKAWEALRVVAKDTCRPFCKDRSGMVLAEGAAVFVLETLESAQ
CCEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCHHHCCCCCEEEECCCCEEHHHHHHHHH
KRGATIHAEMAGFGMTADAGDLLSPSVDGASRAVRMALADGGLAPEDIAYVNAHGTGTVA
HCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHEEEHHHCCCCCCCCEEEEECCCCCCCC
NDATETKCLHEVFGDHAHKLMISATKSFHGHSLGASGAVELVATILALRDGLIAPTLNFT
CCCHHHHHHHHHHCCHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
EPDPECDLDYVPNVARQVTVDAGISNSFAFGGLNAVLALRRFVG
CCCCCCCCCCCCCHHHEEEEECCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: None [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} [C]

Substrates: acetyl-ACP; malonyl-ACP; acyl-ACP [C]

Specific reaction: acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 [C]

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl-ACP-decarboxylation, Cerulenin [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9100384 [H]