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The map label for this gene is gyrB [H]

Identifier: 83591344

GI number: 83591344

Start: 4362

End: 6770

Strand: Direct

Name: gyrB [H]

Synonym: Rru_A0004

Alternate gene names: 83591344

Gene position: 4362-6770 (Clockwise)

Preceding gene: 83591343

Following gene: 83591345

Centisome position: 0.1

GC content: 64.96

Gene sequence:

>2409_bases
ATGACCCAGCAGGCCTATACATCCGAATCCATCAAGGTTCTCAAAGGACTGGAGGCGGTGCGCAAGCGCCCCGGCATGTA
CATCGGCGATACCGACGACGGGTCAGGCCTGCACCACATGGTCTATGAGGTGGTTGATAACGCCATCGACGAGGCGCTCG
CCGGTTACTGCGATCACTCCGAGGTCATTCTCAACGGCGATGGCTCGGTGACGGTCTGTGACAATGGCCGCGGCATCCCC
GTCGATATCCACCGCGAGGAGGGGGTGAGCGCCGCCGAGGTGATCATGACCCAGCTGCATGCCGGCGGAAAGTTCGACCA
GAATTCCTACAAGGTGTCGGGCGGGCTGCACGGCGTGGGCGTATCGGTGGTCAACGCCCTGTCGGACTGGCTTGATCTGC
GCATCTGGCGCGACGGCAAGGAACACTACGTGCGCTTCGAGCACGGCGAGGTGGTGGCGCCGCTGAAGGTGGTGGGCGAC
TGCGGCGACCGCACCGGCACCGAGATCACCTTTTTGGCCTCGCCGGCGATCTTCGCCATCACCACCTATGATTACGCCAC
CCTGGAGCATCGCCTGCGCGAGCTGGCCTTCCTCAATTCCGGGGTGCGGCTGCTGTTGACCGACAAGCGCGGCACCGAGC
CGCGCACCACCAAATTCCACTACGACGGCGGCATCGAGGAATTCGTCCGCTATCTCGACAAGTCGAAAACCGTTCTGCAC
AGCCCGCCGGTCACGGCGATCGGCGAACGCGACGGCATGATCGTGGAACTGTCGCTGGAATGGACCGACAGCTACCACGA
GACGGTGCTGTGCTTCACCAACAACATCCCCCAGCGCGATGGCGGCACCCATATGGCCGGCTTCCGCGGCGGTCTGACCC
GCACCCTCAACAGCTACGCCCAAGACAGCGGGCTGATGAAAAAGGAAAAGGTGACGCTGACCGGCGATGACATGCGCGAG
GGGTTGACCTGCGTGCTGTCGGTCAAGGTTCCCGATCCCAAGTTCTCCAGCCAGACCAAGGAAAAGCTGGTTTCAAGCGA
GGTTCGGCCGGTGGTCGAGGCCGTGGTCGTCGAGCGTCTGGGCCAGTGGCTGGAGGAACACCCTCAGGAGGCCCGCAAGA
TCATCGGCAAGGTGGTGGAAGCCGCCGCCGCCCGCGAGGCCGCCCGCCGCGCCCGCGAACTGACCCGGCGCAAGGGCGCG
CTCGACATGGCCAGCCTGCCCGGCAAGCTGGCCGACTGTCAGAACCGCGATCCGGCCCAGTCCGAACTGTTCATCGTCGA
GGGAGACTCCGCCGGCGGCTCGGCCAAGCAGGGGCGCGATCGCGGCTATCAGGCGATCTTGCCGTTGCGCGGCAAGATCC
TCAACGTCGAGCGGGCGCGCTTTGACAAGATGCTATCCTCGGCGGAAATCGGCACGCTGATCGCCGCGCTTGGCACCGGC
ATCGGCAAGGATGATTTCGATGTCGGCAAGGCCCGCTACCACAAGATCATCATCATGACCGACGCCGACGTCGACGGCAG
CCATATCCGCACCCTGCTGTTGACCTTCTTCTTCCGCCAGATGCCCGAGGTCGTCGAGAAGGGCTATCTTTATATCGCCC
AGCCGCCGCTGTATCGGGCCAAGCGCGGCAATTCCGAGCAGTACCTCAAGGACGACCGCGAGCTTGAGGACTATCTGCTG
GATACCGGCCTGGATGAGACCGTCTACACCACCGCCGCCGGCGTGCAGATGGCCGGTCCCGATCTGCGCGCCCTGGTCGA
ACGCGCCCGCCTTGCGCGCAACGCCATCGCCGCCATGGGCCGCAAGGTGCCGTGGGTCTTGCTTGAACAGGCGGCGGTCG
CCGGGGCCTTCCATACCGAGATCGTCAATGACGACGCCCGCGCCAGCGCCGCGGCCGCCGATGTCGCCCGCCGGATCGAC
GCCCTGGAAAGCCCGCTGGAACGCGGCTGGACCGGCACCCTGGCCGCCGGGGGCGGCATGGTGTTCCAGCGCACGCTGCG
CGGGGTGACCGAGACCCATGTCATCGATCCCCAGGTCATCCACAGCACCGAGGCCCGCCGGCTTGACTCCATGGCGGCCG
CCTTGCGCGAATTCTATGAGCAGCCCGGTCGCCTGCGGATCAAGGACGGCGACCAGATCATCCCCGGCCCGATGACCTTG
ATCAACGCCGTGATGGCCGCCGGCCGCAAGGGCATCGCCATCCAGCGCTATAAGGGCCTGGGCGAGATGAACCCCGAGCA
GCTGTGGGAAACCACCCTTGATCCCAATGTGCGCCGCCTGCTGCAGGTGAAGGTCAATCAGGTCAGCGACGCCGAGGATG
TGTTCAGCACCCTGATGGGCGACATCGTCGAACCCCGCCGCGACTTTATCCAAGACAACGCCCTGAACGTCCAGAACCTG
GACGTTTAA

Upstream 100 bases:

>100_bases
TTCCTGCGCATCGCCGAGGGGGCCGTGCTAGACTAGCAAAGCCCCCGTTGCCGGCTTTCGGGCCGGACCTTTCCACGTAG
ACAACAAGAAGACCGCTCCC

Downstream 100 bases:

>100_bases
GGCAAATCCCGAGCGATCTGAACCAGATCGCGACGACGATTTGCTTCGCGATCAAGATCTTAGAGCGGTGTGCGGGGACC
ATATTTCCCCCACACCGCCC

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 802; Mature: 801

Protein sequence:

>802_residues
MTQQAYTSESIKVLKGLEAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYCDHSEVILNGDGSVTVCDNGRGIP
VDIHREEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLDLRIWRDGKEHYVRFEHGEVVAPLKVVGD
CGDRTGTEITFLASPAIFAITTYDYATLEHRLRELAFLNSGVRLLLTDKRGTEPRTTKFHYDGGIEEFVRYLDKSKTVLH
SPPVTAIGERDGMIVELSLEWTDSYHETVLCFTNNIPQRDGGTHMAGFRGGLTRTLNSYAQDSGLMKKEKVTLTGDDMRE
GLTCVLSVKVPDPKFSSQTKEKLVSSEVRPVVEAVVVERLGQWLEEHPQEARKIIGKVVEAAAAREAARRARELTRRKGA
LDMASLPGKLADCQNRDPAQSELFIVEGDSAGGSAKQGRDRGYQAILPLRGKILNVERARFDKMLSSAEIGTLIAALGTG
IGKDDFDVGKARYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVVEKGYLYIAQPPLYRAKRGNSEQYLKDDRELEDYLL
DTGLDETVYTTAAGVQMAGPDLRALVERARLARNAIAAMGRKVPWVLLEQAAVAGAFHTEIVNDDARASAAAADVARRID
ALESPLERGWTGTLAAGGGMVFQRTLRGVTETHVIDPQVIHSTEARRLDSMAAALREFYEQPGRLRIKDGDQIIPGPMTL
INAVMAAGRKGIAIQRYKGLGEMNPEQLWETTLDPNVRRLLQVKVNQVSDAEDVFSTLMGDIVEPRRDFIQDNALNVQNL
DV

Sequences:

>Translated_802_residues
MTQQAYTSESIKVLKGLEAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYCDHSEVILNGDGSVTVCDNGRGIP
VDIHREEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLDLRIWRDGKEHYVRFEHGEVVAPLKVVGD
CGDRTGTEITFLASPAIFAITTYDYATLEHRLRELAFLNSGVRLLLTDKRGTEPRTTKFHYDGGIEEFVRYLDKSKTVLH
SPPVTAIGERDGMIVELSLEWTDSYHETVLCFTNNIPQRDGGTHMAGFRGGLTRTLNSYAQDSGLMKKEKVTLTGDDMRE
GLTCVLSVKVPDPKFSSQTKEKLVSSEVRPVVEAVVVERLGQWLEEHPQEARKIIGKVVEAAAAREAARRARELTRRKGA
LDMASLPGKLADCQNRDPAQSELFIVEGDSAGGSAKQGRDRGYQAILPLRGKILNVERARFDKMLSSAEIGTLIAALGTG
IGKDDFDVGKARYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVVEKGYLYIAQPPLYRAKRGNSEQYLKDDRELEDYLL
DTGLDETVYTTAAGVQMAGPDLRALVERARLARNAIAAMGRKVPWVLLEQAAVAGAFHTEIVNDDARASAAAADVARRID
ALESPLERGWTGTLAAGGGMVFQRTLRGVTETHVIDPQVIHSTEARRLDSMAAALREFYEQPGRLRIKDGDQIIPGPMTL
INAVMAAGRKGIAIQRYKGLGEMNPEQLWETTLDPNVRRLLQVKVNQVSDAEDVFSTLMGDIVEPRRDFIQDNALNVQNL
DV
>Mature_801_residues
TQQAYTSESIKVLKGLEAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYCDHSEVILNGDGSVTVCDNGRGIPV
DIHREEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSDWLDLRIWRDGKEHYVRFEHGEVVAPLKVVGDC
GDRTGTEITFLASPAIFAITTYDYATLEHRLRELAFLNSGVRLLLTDKRGTEPRTTKFHYDGGIEEFVRYLDKSKTVLHS
PPVTAIGERDGMIVELSLEWTDSYHETVLCFTNNIPQRDGGTHMAGFRGGLTRTLNSYAQDSGLMKKEKVTLTGDDMREG
LTCVLSVKVPDPKFSSQTKEKLVSSEVRPVVEAVVVERLGQWLEEHPQEARKIIGKVVEAAAAREAARRARELTRRKGAL
DMASLPGKLADCQNRDPAQSELFIVEGDSAGGSAKQGRDRGYQAILPLRGKILNVERARFDKMLSSAEIGTLIAALGTGI
GKDDFDVGKARYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVVEKGYLYIAQPPLYRAKRGNSEQYLKDDRELEDYLLD
TGLDETVYTTAAGVQMAGPDLRALVERARLARNAIAAMGRKVPWVLLEQAAVAGAFHTEIVNDDARASAAAADVARRIDA
LESPLERGWTGTLAAGGGMVFQRTLRGVTETHVIDPQVIHSTEARRLDSMAAALREFYEQPGRLRIKDGDQIIPGPMTLI
NAVMAAGRKGIAIQRYKGLGEMNPEQLWETTLDPNVRRLLQVKVNQVSDAEDVFSTLMGDIVEPRRDFIQDNALNVQNLD
V

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=575, Percent_Identity=25.0434782608696, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI19913406, Length=592, Percent_Identity=23.4797297297297, Blast_Score=113, Evalue=6e-25,
Organism=Escherichia coli, GI48994957, Length=812, Percent_Identity=55.4187192118227, Blast_Score=845, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=559, Percent_Identity=41.1449016100179, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535065, Length=590, Percent_Identity=23.8983050847458, Blast_Score=125, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=515, Percent_Identity=23.495145631068, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=161, Percent_Identity=31.055900621118, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=581, Percent_Identity=24.9569707401033, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI17136538, Length=599, Percent_Identity=25.3756260434057, Blast_Score=133, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 88341; Mature: 88210

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQAYTSESIKVLKGLEAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYCDHS
CCCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EVILNGDGSVTVCDNGRGIPVDIHREEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVG
CEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSDWLDLRIWRDGKEHYVRFEHGEVVAPLKVVGDCGDRTGTEITFLASPAIFAI
HHHHHHHHHHHHEEEEECCHHHEEEEECCCEEEHHHHHCCCCCCCCCEEEEEECCCEEEE
TTYDYATLEHRLRELAFLNSGVRLLLTDKRGTEPRTTKFHYDGGIEEFVRYLDKSKTVLH
EECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCEEEE
SPPVTAIGERDGMIVELSLEWTDSYHETVLCFTNNIPQRDGGTHMAGFRGGLTRTLNSYA
CCCCCEECCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHH
QDSGLMKKEKVTLTGDDMREGLTCVLSVKVPDPKFSSQTKEKLVSSEVRPVVEAVVVERL
HHCCCCCCCEEEECCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GQWLEEHPQEARKIIGKVVEAAAAREAARRARELTRRKGALDMASLPGKLADCQNRDPAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCC
SELFIVEGDSAGGSAKQGRDRGYQAILPLRGKILNVERARFDKMLSSAEIGTLIAALGTG
CEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGKDDFDVGKARYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVVEKGYLYIAQPPLYRA
CCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
KRGNSEQYLKDDRELEDYLLDTGLDETVYTTAAGVQMAGPDLRALVERARLARNAIAAMG
CCCCCHHHHHCHHHHHHHHHHCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHC
RKVPWVLLEQAAVAGAFHTEIVNDDARASAAAADVARRIDALESPLERGWTGTLAAGGGM
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
VFQRTLRGVTETHVIDPQVIHSTEARRLDSMAAALREFYEQPGRLRIKDGDQIIPGPMTL
HHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCHHHH
INAVMAAGRKGIAIQRYKGLGEMNPEQLWETTLDPNVRRLLQVKVNQVSDAEDVFSTLMG
HHHHHHCCCCCCEEHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
DIVEPRRDFIQDNALNVQNLDV
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TQQAYTSESIKVLKGLEAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAGYCDHS
CCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EVILNGDGSVTVCDNGRGIPVDIHREEGVSAAEVIMTQLHAGGKFDQNSYKVSGGLHGVG
CEEEECCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHH
VSVVNALSDWLDLRIWRDGKEHYVRFEHGEVVAPLKVVGDCGDRTGTEITFLASPAIFAI
HHHHHHHHHHHHEEEEECCHHHEEEEECCCEEEHHHHHCCCCCCCCCEEEEEECCCEEEE
TTYDYATLEHRLRELAFLNSGVRLLLTDKRGTEPRTTKFHYDGGIEEFVRYLDKSKTVLH
EECHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCEEEE
SPPVTAIGERDGMIVELSLEWTDSYHETVLCFTNNIPQRDGGTHMAGFRGGLTRTLNSYA
CCCCCEECCCCCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCHHCCCHHHHHHHHH
QDSGLMKKEKVTLTGDDMREGLTCVLSVKVPDPKFSSQTKEKLVSSEVRPVVEAVVVERL
HHCCCCCCCEEEECCCHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
GQWLEEHPQEARKIIGKVVEAAAAREAARRARELTRRKGALDMASLPGKLADCQNRDPAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCCCC
SELFIVEGDSAGGSAKQGRDRGYQAILPLRGKILNVERARFDKMLSSAEIGTLIAALGTG
CEEEEEECCCCCCCCCCCCCCCCEEEECCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGKDDFDVGKARYHKIIIMTDADVDGSHIRTLLLTFFFRQMPEVVEKGYLYIAQPPLYRA
CCCCCCCCCCHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHH
KRGNSEQYLKDDRELEDYLLDTGLDETVYTTAAGVQMAGPDLRALVERARLARNAIAAMG
CCCCCHHHHHCHHHHHHHHHHCCCCCHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHC
RKVPWVLLEQAAVAGAFHTEIVNDDARASAAAADVARRIDALESPLERGWTGTLAAGGGM
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHH
VFQRTLRGVTETHVIDPQVIHSTEARRLDSMAAALREFYEQPGRLRIKDGDQIIPGPMTL
HHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEECCCHHHH
INAVMAAGRKGIAIQRYKGLGEMNPEQLWETTLDPNVRRLLQVKVNQVSDAEDVFSTLMG
HHHHHHCCCCCCEEHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
DIVEPRRDFIQDNALNVQNLDV
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]