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The map label for this gene is manX [H]

Identifier: 83313494

GI number: 83313494

Start: 4797783

End: 4798253

Strand: Reverse

Name: manX [H]

Synonym: amb4395

Alternate gene names: 83313494

Gene position: 4798253-4797783 (Counterclockwise)

Preceding gene: 83313495

Following gene: 83313493

Centisome position: 96.6

GC content: 65.82

Gene sequence:

>471_bases
GTGCAAGACGCGCGGGATCGGGGCCGGTGGGGCTGGCCTTTTGCCCGTTCAAGGGGGATTGAGATGATCGGACTGGTACT
CGTCACCCACGGCCGGCTCGCCGACGAACTCGTCGCCGCGCTCGAGCACGTGGTGGGGCCGCAGCCCAATGTCGGGGCGG
TATGCATCGGGCCCGACGACGACATGGAGCAGCGGCGCAACGATATCCTGTCCAGCGTGGCGCGCTGTGACGACGGCGCC
GGCGTGGTGGTGCTGACCGACATGTTCGGCGGCACGCCGTCCAATCTGGCCATTTCCATCATGGACAAGGCCAAGGTCGA
GGTGATCGCAGGCGTCAACCTGCCCATGCTGATCAAACTGGCCAGCGTGCGCCATTCCGAGGCCCTGGCCGATGCCGTGG
CCAGCGCCCAGGAGGCCGGTCGCAAATACATCAACGTCGCCTCCAAGCTGCTGACTCAGGATCGCAAGTAA

Upstream 100 bases:

>100_bases
GTCGCCATCGCCGAGCACCTAGCGCAGTGGATGCAACGACGGGGCGGAAAGGTCGAGCTTCGCCATCGCGAACTCGACGA
ACGGGGTTCATGATGTCACT

Downstream 100 bases:

>100_bases
TGTCGGACGTGATCGGAACCTCGGGCGGGGCCGGCGCGGAGCTGGTCCGCACCGCCACCATCTCCAACCGACGCGGTCTG
CACGCCCGGGCCGCCGCCAA

Product: phosphotransferase system, mannose/fructose-specific component IIA

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MQDARDRGRWGWPFARSRGIEMIGLVLVTHGRLADELVAALEHVVGPQPNVGAVCIGPDDDMEQRRNDILSSVARCDDGA
GVVVLTDMFGGTPSNLAISIMDKAKVEVIAGVNLPMLIKLASVRHSEALADAVASAQEAGRKYINVASKLLTQDRK

Sequences:

>Translated_156_residues
MQDARDRGRWGWPFARSRGIEMIGLVLVTHGRLADELVAALEHVVGPQPNVGAVCIGPDDDMEQRRNDILSSVARCDDGA
GVVVLTDMFGGTPSNLAISIMDKAKVEVIAGVNLPMLIKLASVRHSEALADAVASAQEAGRKYINVASKLLTQDRK
>Mature_156_residues
MQDARDRGRWGWPFARSRGIEMIGLVLVTHGRLADELVAALEHVVGPQPNVGAVCIGPDDDMEQRRNDILSSVARCDDGA
GVVVLTDMFGGTPSNLAISIMDKAKVEVIAGVNLPMLIKLASVRHSEALADAVASAQEAGRKYINVASKLLTQDRK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=122, Percent_Identity=37.7049180327869, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 16715; Mature: 16715

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDARDRGRWGWPFARSRGIEMIGLVLVTHGRLADELVAALEHVVGPQPNVGAVCIGPDD
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCC
DMEQRRNDILSSVARCDDGAGVVVLTDMFGGTPSNLAISIMDKAKVEVIAGVNLPMLIKL
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHH
ASVRHSEALADAVASAQEAGRKYINVASKLLTQDRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQDARDRGRWGWPFARSRGIEMIGLVLVTHGRLADELVAALEHVVGPQPNVGAVCIGPDD
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCC
DMEQRRNDILSSVARCDDGAGVVVLTDMFGGTPSNLAISIMDKAKVEVIAGVNLPMLIKL
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCHHHHHH
ASVRHSEALADAVASAQEAGRKYINVASKLLTQDRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]