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The map label for this gene is pheS [H]

Identifier: 83313480

GI number: 83313480

Start: 4784626

End: 4785357

Strand: Reverse

Name: pheS [H]

Synonym: amb4381

Alternate gene names: 83313480

Gene position: 4785357-4784626 (Counterclockwise)

Preceding gene: 83313481

Following gene: 83313479

Centisome position: 96.34

GC content: 63.25

Gene sequence:

>732_bases
ATGAGCGCCATCTTCGCCCAGATGGGCTTCGATGTGGCGGAAGGCCCGGACATCGAGGACGATTTCCACAATTTCAACGC
GCTGAACATTCCGCCCGAGCATCCGGCGCGGCAGATGCACGACACCTTCTATTTCGGCGAGAAGCCCGACGGGGGGCGCC
ATCTGCTGCGCACCCATACCAGCCCGGTGCAGATCCGCACCATGCTGGCCAACAAGCCGCCGATCCGCATCATCGCCCCG
GGCCGTACCTATCGCTGCGATTCGGATATGACCCACACGCCCATGTTCCATCAGATCGAGGGCCTGGTCATCGACGAGAT
CACCCATATGGGCCATCTCAAGGGCTGCCTGCTGGAATTCGTCCGCGCCTTCTTTGAGGTGGACGACGTGCCGGTGCGCT
TCCGCCCCAGCTTCTTCCCCTTCACCGAGCCCTCGGCCGAGGTGGATATCGGCTGTTCGCGCGAAGGCGGCGAACTGAAG
ATCGGACCCGGCGCCGGCTGGCTGGAAATCGGCGGCTCGGGCATGGTCCATCCCAATGTGCTGCGCAACTGCGGCATCGA
CCCCGACCGCTACCAGGGCTTCGCCTTCGGCATGGGCGTCGAGCGCATGGCCATGCTGAAATACGGCATTCCCGACCTGC
GCACCTTCTTTGATTCCGATCTGCGCTGGCTGAAGCATTACGGCTTTGTGCCGCTGGACGTTCCCACGCTTTCGGGAGGG
CTGACCCGATGA

Upstream 100 bases:

>100_bases
CCTGGACGAGCGTCTGGCCCGTGAACGCATCGACGTCACCCTGCCGTCGCGGCCCGAATCCCTGGGGCGTATTCACCCCA
TTTCCCAGGTCATGGACGAG

Downstream 100 bases:

>100_bases
AGTTCACCCTTGATTGGCTGAAAAGCCATCTGGACACCGACGCGACCCTGGAACAGATCGAGGTGGGTCTGACCGCCATC
GGCCTGGAGGTCGAGGGCAT

Product: phenylalanyl-tRNA synthetase alpha subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSAIFAQMGFDVAEGPDIEDDFHNFNALNIPPEHPARQMHDTFYFGEKPDGGRHLLRTHTSPVQIRTMLANKPPIRIIAP
GRTYRCDSDMTHTPMFHQIEGLVIDEITHMGHLKGCLLEFVRAFFEVDDVPVRFRPSFFPFTEPSAEVDIGCSREGGELK
IGPGAGWLEIGGSGMVHPNVLRNCGIDPDRYQGFAFGMGVERMAMLKYGIPDLRTFFDSDLRWLKHYGFVPLDVPTLSGG
LTR

Sequences:

>Translated_243_residues
MSAIFAQMGFDVAEGPDIEDDFHNFNALNIPPEHPARQMHDTFYFGEKPDGGRHLLRTHTSPVQIRTMLANKPPIRIIAP
GRTYRCDSDMTHTPMFHQIEGLVIDEITHMGHLKGCLLEFVRAFFEVDDVPVRFRPSFFPFTEPSAEVDIGCSREGGELK
IGPGAGWLEIGGSGMVHPNVLRNCGIDPDRYQGFAFGMGVERMAMLKYGIPDLRTFFDSDLRWLKHYGFVPLDVPTLSGG
LTR
>Mature_242_residues
SAIFAQMGFDVAEGPDIEDDFHNFNALNIPPEHPARQMHDTFYFGEKPDGGRHLLRTHTSPVQIRTMLANKPPIRIIAPG
RTYRCDSDMTHTPMFHQIEGLVIDEITHMGHLKGCLLEFVRAFFEVDDVPVRFRPSFFPFTEPSAEVDIGCSREGGELKI
GPGAGWLEIGGSGMVHPNVLRNCGIDPDRYQGFAFGMGVERMAMLKYGIPDLRTFFDSDLRWLKHYGFVPLDVPTLSGGL
TR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=256, Percent_Identity=35.15625, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI5729820, Length=242, Percent_Identity=29.7520661157025, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1788007, Length=227, Percent_Identity=60.7929515418502, Blast_Score=282, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI32563657, Length=258, Percent_Identity=31.0077519379845, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=258, Percent_Identity=31.0077519379845, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32566635, Length=262, Percent_Identity=29.0076335877863, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321087, Length=255, Percent_Identity=31.7647058823529, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325304, Length=100, Percent_Identity=33, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18858079, Length=253, Percent_Identity=31.2252964426877, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 27295; Mature: 27163

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIFAQMGFDVAEGPDIEDDFHNFNALNIPPEHPARQMHDTFYFGEKPDGGRHLLRTHT
CCCHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHCCC
SPVQIRTMLANKPPIRIIAPGRTYRCDSDMTHTPMFHQIEGLVIDEITHMGHLKGCLLEF
CCCEEEEECCCCCCEEEECCCCEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VRAFFEVDDVPVRFRPSFFPFTEPSAEVDIGCSREGGELKIGPGAGWLEIGGSGMVHPNV
HHHHHCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCEEEECCCCCEEEECCCCCCCHHH
LRNCGIDPDRYQGFAFGMGVERMAMLKYGIPDLRTFFDSDLRWLKHYGFVPLDVPTLSGG
HHHCCCCHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
LTR
CCC
>Mature Secondary Structure 
SAIFAQMGFDVAEGPDIEDDFHNFNALNIPPEHPARQMHDTFYFGEKPDGGRHLLRTHT
CCHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHEECCCCCCCCCHHHHCCC
SPVQIRTMLANKPPIRIIAPGRTYRCDSDMTHTPMFHQIEGLVIDEITHMGHLKGCLLEF
CCCEEEEECCCCCCEEEECCCCEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VRAFFEVDDVPVRFRPSFFPFTEPSAEVDIGCSREGGELKIGPGAGWLEIGGSGMVHPNV
HHHHHCCCCCCEEECCCCCCCCCCCCCEECCCCCCCCEEEECCCCCEEEECCCCCCCHHH
LRNCGIDPDRYQGFAFGMGVERMAMLKYGIPDLRTFFDSDLRWLKHYGFVPLDVPTLSGG
HHHCCCCHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCC
LTR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA