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The map label for this gene is pheT

Identifier: 83313479

GI number: 83313479

Start: 4782233

End: 4784629

Strand: Reverse

Name: pheT

Synonym: amb4380

Alternate gene names: 83313479

Gene position: 4784629-4782233 (Counterclockwise)

Preceding gene: 83313480

Following gene: 83313478

Centisome position: 96.33

GC content: 68.17

Gene sequence:

>2397_bases
ATGAAGTTCACCCTTGATTGGCTGAAAAGCCATCTGGACACCGACGCGACCCTGGAACAGATCGAGGTGGGTCTGACCGC
CATCGGCCTGGAGGTCGAGGGCATCGACGACCCGTCCAAGCATCTGGGCGGCTTCGTGGTCGGCCATATCCTGGAAGCCG
AGAAGCATCCGGATGCCGACAAGCTGAAGCTGTGCAAGGTGGACAGCGGCGCAGGGATTCTTCAAGTGGTGTGCGGCGCC
CCCAATGCGCGCGCCGGGCTGAAAGTCATCCTGGCCCAGCCGGGCACCTACATCCCCATCACCGGCGACGTCCTGAAGAA
GGGCAAGGTCCGCGGCGTGGAAAGCCAGGGCATGATGTGCTCGTGGCGCGAACTCAAGCTGGGCGAGGACCACGACGGCA
TCGCCGAGCTGGATGTCACCCTTGCGGTGGGCGCCAGCCTGCTCGACATCATGAGCTTCGATCCCATGATCGAGATCTCG
GTCACTCCCAACCGCGTCGATTGCCTGGGCGTGCGCGGCATCGCCCGCGATCTGGCGGCCTTCGGTCTGGGCAACCTCAA
GCCCCTGAAGGTCGAGCCGGTGCCCGGCGCCTTCAAAAGCTCCATCGGCGTGCGCCTGGAGTTCGCGCCCGAGGACTCCA
ACGCTTGCCCGCTGTTCGCCGGCCGCCTGATCCGGGGCGTCAAGAACGGCGACAGCCCGCAATGGCTGAAGGACCGCCTG
ACCGCCATCGGGCTTCGCCCCATCTCGGCCCTGGTGGACATCACCAATTTCTTCGCCTACGACCTGTGCCGCCCGCTGCA
CGTCTTCGACGCCGCCAAGGTCAAGGGCGACATCCGCGCCCGTCTGGCCCGTGACGGCGAGACCCTGGCCGGTCTCAACG
GCAAGACCTACACGCTGGAGTCCGGCATGACGGTCATCGCTGACGAGAACGGACCCGAGGCCCTGGCCGGCATCCTGGGC
GGCGAGCATTCCGGCTGCACCGAGGCCACCACCGAGGTGTTCCTGGAAGCCGCCTATTTCGACCCCATCCGTACCGCCGC
CACCGGGCGCAAGCTGGATATCCTGTCGGACGCCCGCTTCCGCTTCGAGCGCGGCGTCGATCCGGCCTTCGTGGTGCCGT
CCATGGAGCTGGCCACCCGCATGATCCTCGATCTGTGCGGCGGCGAGGCCTCGGAGGCGGTGATCGCCGGCACCGAGCCC
GATTGGCAGAAATCCATCGTGCTGCGCCCCAATCGCGTCGCCGAACTGGGCGGCGTCGAGGTGTCGACCCAGCGCATGGA
GACCATCCTCAACGACCTGGGCTGCGCCGTGGCCGAGCATGCGGACGGCCTGCTGGTCAATCCGCCGTCATGGCGCGGCG
ACATCACCGCCGAGCACGATCTGGTGGAAGAGGTCATCCGCATCAACGGCTATGACAACATCCCCGCCACTCCCATGCCG
CGTCCGGCCATGCCCAAGCCGGTGCTGACCCCCGGCCAGCGCCGTTCCGGCTGGGTGCGCCGCCAGTTGGCCACCCGTGG
GCTGGTGGAAACGGTCACCTGGTCGTTCCTGCCCGAGGCCCAGGCCGTGATGTTCGGCGGCGGCGCCCCGGAAATGCACT
TGGCCAATCCCATCTCGTCCGATCTCGACGTGATGCGTCCCTCGGTGCTGCCCAATCTGGTGACGGCGGCCGGGCGCAAT
GCCGATCGCGGCATGAAGGACCTGGGCCTGTTCGAACTGGGGCCGCAATTCGACGGACCCGAGCCGGGGCAGCAGCGTCT
GGTGGCGGCCGGCATTCGAGCCGGCAGGGCGCGCGGCCGTCACTGGGCCGATCCGGCCCGCGCCGTGGATGCTTTCGACG
CCAAGGCCGACATCCTGTCCGCCGTGGCGGCCGCCGGGGCCAATCCCGACTCGCTGCAGGTGGTTGCCGAGGCGCCCGTC
TGGTACCACCCCGGCCGCTCGGGCACGCTCAAGCTGGGCAACAAGCCGGTGGGCTTCTTCGGCGAGATCCACCCCGGCCT
TCTGGCCAAGCTGGACGTCAAGGGTCCGGTGGCCGGCTTCGAGCTGTTCCTGGAGGCCCTGCCGGCGCAGAAGGCCAAGG
CCACCAAGGCCAAGCCGCTGCTCAAGGCCTCGGCCTTGCAGCCCCTGGACCGCGACTTCGCCTTCACCCTGGATACGGGC
GTGGCGGCGGATGCGGTGGTGCGGGCCGCCCGCAATGCCGACAAGGTTCTGATCAGCGACGTGGCGGTGTTCGACCTCTA
CGAGGGCGACAAGATGGCGGCGGGCAAGAAGTCCCTGGCCATCACCGTGACGCTGCAGCCCACCGACAAGACCCTGACCG
ACGAGGACATCGAGGCGGTGGGGGCGAAGATCGTCGCGGCGGTGGCCAAGGCCACCGGCGGCGAGCTTCGGGGCTGA

Upstream 100 bases:

>100_bases
TTCCCGACCTGCGCACCTTCTTTGATTCCGATCTGCGCTGGCTGAAGCATTACGGCTTTGTGCCGCTGGACGTTCCCACG
CTTTCGGGAGGGCTGACCCG

Downstream 100 bases:

>100_bases
TCCCAGGCCGTGCCGACCGTCATCCTGGTCCGCCACGGCGAGACTGTCTGGAACCGCGAGGGCCGCGTCCAGGGTCACGG
CGATTCGCCGCTGACCCCCA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 798; Mature: 798

Protein sequence:

>798_residues
MKFTLDWLKSHLDTDATLEQIEVGLTAIGLEVEGIDDPSKHLGGFVVGHILEAEKHPDADKLKLCKVDSGAGILQVVCGA
PNARAGLKVILAQPGTYIPITGDVLKKGKVRGVESQGMMCSWRELKLGEDHDGIAELDVTLAVGASLLDIMSFDPMIEIS
VTPNRVDCLGVRGIARDLAAFGLGNLKPLKVEPVPGAFKSSIGVRLEFAPEDSNACPLFAGRLIRGVKNGDSPQWLKDRL
TAIGLRPISALVDITNFFAYDLCRPLHVFDAAKVKGDIRARLARDGETLAGLNGKTYTLESGMTVIADENGPEALAGILG
GEHSGCTEATTEVFLEAAYFDPIRTAATGRKLDILSDARFRFERGVDPAFVVPSMELATRMILDLCGGEASEAVIAGTEP
DWQKSIVLRPNRVAELGGVEVSTQRMETILNDLGCAVAEHADGLLVNPPSWRGDITAEHDLVEEVIRINGYDNIPATPMP
RPAMPKPVLTPGQRRSGWVRRQLATRGLVETVTWSFLPEAQAVMFGGGAPEMHLANPISSDLDVMRPSVLPNLVTAAGRN
ADRGMKDLGLFELGPQFDGPEPGQQRLVAAGIRAGRARGRHWADPARAVDAFDAKADILSAVAAAGANPDSLQVVAEAPV
WYHPGRSGTLKLGNKPVGFFGEIHPGLLAKLDVKGPVAGFELFLEALPAQKAKATKAKPLLKASALQPLDRDFAFTLDTG
VAADAVVRAARNADKVLISDVAVFDLYEGDKMAAGKKSLAITVTLQPTDKTLTDEDIEAVGAKIVAAVAKATGGELRG

Sequences:

>Translated_798_residues
MKFTLDWLKSHLDTDATLEQIEVGLTAIGLEVEGIDDPSKHLGGFVVGHILEAEKHPDADKLKLCKVDSGAGILQVVCGA
PNARAGLKVILAQPGTYIPITGDVLKKGKVRGVESQGMMCSWRELKLGEDHDGIAELDVTLAVGASLLDIMSFDPMIEIS
VTPNRVDCLGVRGIARDLAAFGLGNLKPLKVEPVPGAFKSSIGVRLEFAPEDSNACPLFAGRLIRGVKNGDSPQWLKDRL
TAIGLRPISALVDITNFFAYDLCRPLHVFDAAKVKGDIRARLARDGETLAGLNGKTYTLESGMTVIADENGPEALAGILG
GEHSGCTEATTEVFLEAAYFDPIRTAATGRKLDILSDARFRFERGVDPAFVVPSMELATRMILDLCGGEASEAVIAGTEP
DWQKSIVLRPNRVAELGGVEVSTQRMETILNDLGCAVAEHADGLLVNPPSWRGDITAEHDLVEEVIRINGYDNIPATPMP
RPAMPKPVLTPGQRRSGWVRRQLATRGLVETVTWSFLPEAQAVMFGGGAPEMHLANPISSDLDVMRPSVLPNLVTAAGRN
ADRGMKDLGLFELGPQFDGPEPGQQRLVAAGIRAGRARGRHWADPARAVDAFDAKADILSAVAAAGANPDSLQVVAEAPV
WYHPGRSGTLKLGNKPVGFFGEIHPGLLAKLDVKGPVAGFELFLEALPAQKAKATKAKPLLKASALQPLDRDFAFTLDTG
VAADAVVRAARNADKVLISDVAVFDLYEGDKMAAGKKSLAITVTLQPTDKTLTDEDIEAVGAKIVAAVAKATGGELRG
>Mature_798_residues
MKFTLDWLKSHLDTDATLEQIEVGLTAIGLEVEGIDDPSKHLGGFVVGHILEAEKHPDADKLKLCKVDSGAGILQVVCGA
PNARAGLKVILAQPGTYIPITGDVLKKGKVRGVESQGMMCSWRELKLGEDHDGIAELDVTLAVGASLLDIMSFDPMIEIS
VTPNRVDCLGVRGIARDLAAFGLGNLKPLKVEPVPGAFKSSIGVRLEFAPEDSNACPLFAGRLIRGVKNGDSPQWLKDRL
TAIGLRPISALVDITNFFAYDLCRPLHVFDAAKVKGDIRARLARDGETLAGLNGKTYTLESGMTVIADENGPEALAGILG
GEHSGCTEATTEVFLEAAYFDPIRTAATGRKLDILSDARFRFERGVDPAFVVPSMELATRMILDLCGGEASEAVIAGTEP
DWQKSIVLRPNRVAELGGVEVSTQRMETILNDLGCAVAEHADGLLVNPPSWRGDITAEHDLVEEVIRINGYDNIPATPMP
RPAMPKPVLTPGQRRSGWVRRQLATRGLVETVTWSFLPEAQAVMFGGGAPEMHLANPISSDLDVMRPSVLPNLVTAAGRN
ADRGMKDLGLFELGPQFDGPEPGQQRLVAAGIRAGRARGRHWADPARAVDAFDAKADILSAVAAAGANPDSLQVVAEAPV
WYHPGRSGTLKLGNKPVGFFGEIHPGLLAKLDVKGPVAGFELFLEALPAQKAKATKAKPLLKASALQPLDRDFAFTLDTG
VAADAVVRAARNADKVLISDVAVFDLYEGDKMAAGKKSLAITVTLQPTDKTLTDEDIEAVGAKIVAAVAKATGGELRG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788006, Length=817, Percent_Identity=36.4749082007344, Blast_Score=461, Evalue=1e-131,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_MAGSA (Q2VYZ1)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_423743.1
- ProteinModelPortal:   Q2VYZ1
- SMR:   Q2VYZ1
- STRING:   Q2VYZ1
- GeneID:   3804417
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb4380
- NMPDR:   fig|342108.5.peg.3935
- eggNOG:   COG0072
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- PhylomeDB:   Q2VYZ1
- ProtClustDB:   PRK00629
- BioCyc:   MMAG342108:AMB4380-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 84830; Mature: 84830

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLDWLKSHLDTDATLEQIEVGLTAIGLEVEGIDDPSKHLGGFVVGHILEAEKHPDAD
CCEEHHHHHHHCCCCHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
KLKLCKVDSGAGILQVVCGAPNARAGLKVILAQPGTYIPITGDVLKKGKVRGVESQGMMC
CEEEEEECCCCCEEEEEECCCCCCCCEEEEEECCCCEEEECHHHHCCCCCCCCCCCCCEE
SWRELKLGEDHDGIAELDVTLAVGASLLDIMSFDPMIEISVTPNRVDCLGVRGIARDLAA
EHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHHHH
FGLGNLKPLKVEPVPGAFKSSIGVRLEFAPEDSNACPLFAGRLIRGVKNGDSPQWLKDRL
HCCCCCCCEEECCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
TAIGLRPISALVDITNFFAYDLCRPLHVFDAAKVKGDIRARLARDGETLAGLNGKTYTLE
HHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEC
SGMTVIADENGPEALAGILGGEHSGCTEATTEVFLEAAYFDPIRTAATGRKLDILSDARF
CCCEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCEEEEHHHHHH
RFERGVDPAFVVPSMELATRMILDLCGGEASEAVIAGTEPDWQKSIVLRPNRVAELGGVE
HHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCHHHHCCCE
VSTQRMETILNDLGCAVAEHADGLLVNPPSWRGDITAEHDLVEEVIRINGYDNIPATPMP
EHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
RPAMPKPVLTPGQRRSGWVRRQLATRGLVETVTWSFLPEAQAVMFGGGAPEMHLANPISS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCCCC
DLDVMRPSVLPNLVTAAGRNADRGMKDLGLFELGPQFDGPEPGQQRLVAAGIRAGRARGR
CHHHHCHHHHHHHHHHCCCCCCCCHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
HWADPARAVDAFDAKADILSAVAAAGANPDSLQVVAEAPVWYHPGRSGTLKLGNKPVGFF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCCCCCEEEECCCCCCCC
GEIHPGLLAKLDVKGPVAGFELFLEALPAQKAKATKAKPLLKASALQPLDRDFAFTLDTG
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCCCCEEEEECCC
VAADAVVRAARNADKVLISDVAVFDLYEGDKMAAGKKSLAITVTLQPTDKTLTDEDIEAV
CHHHHHHHHHCCCCEEEEEHEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHH
GAKIVAAVAKATGGELRG
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFTLDWLKSHLDTDATLEQIEVGLTAIGLEVEGIDDPSKHLGGFVVGHILEAEKHPDAD
CCEEHHHHHHHCCCCHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCC
KLKLCKVDSGAGILQVVCGAPNARAGLKVILAQPGTYIPITGDVLKKGKVRGVESQGMMC
CEEEEEECCCCCEEEEEECCCCCCCCEEEEEECCCCEEEECHHHHCCCCCCCCCCCCCEE
SWRELKLGEDHDGIAELDVTLAVGASLLDIMSFDPMIEISVTPNRVDCLGVRGIARDLAA
EHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHHHH
FGLGNLKPLKVEPVPGAFKSSIGVRLEFAPEDSNACPLFAGRLIRGVKNGDSPQWLKDRL
HCCCCCCCEEECCCCCHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
TAIGLRPISALVDITNFFAYDLCRPLHVFDAAKVKGDIRARLARDGETLAGLNGKTYTLE
HHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEC
SGMTVIADENGPEALAGILGGEHSGCTEATTEVFLEAAYFDPIRTAATGRKLDILSDARF
CCCEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCEEEEHHHHHH
RFERGVDPAFVVPSMELATRMILDLCGGEASEAVIAGTEPDWQKSIVLRPNRVAELGGVE
HHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEECCCCHHHHCCCE
VSTQRMETILNDLGCAVAEHADGLLVNPPSWRGDITAEHDLVEEVIRINGYDNIPATPMP
EHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
RPAMPKPVLTPGQRRSGWVRRQLATRGLVETVTWSFLPEAQAVMFGGGAPEMHLANPISS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEECCCCCC
DLDVMRPSVLPNLVTAAGRNADRGMKDLGLFELGPQFDGPEPGQQRLVAAGIRAGRARGR
CHHHHCHHHHHHHHHHCCCCCCCCHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
HWADPARAVDAFDAKADILSAVAAAGANPDSLQVVAEAPVWYHPGRSGTLKLGNKPVGFF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEECCCCCCEEEECCCCCCCC
GEIHPGLLAKLDVKGPVAGFELFLEALPAQKAKATKAKPLLKASALQPLDRDFAFTLDTG
CCCCCCEEEEEECCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHCCCCCCCEEEEECCC
VAADAVVRAARNADKVLISDVAVFDLYEGDKMAAGKKSLAITVTLQPTDKTLTDEDIEAV
CHHHHHHHHHCCCCEEEEEHEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHH
GAKIVAAVAKATGGELRG
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA